BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0307.0006.p (317 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value CAC0076 [GER] COG0697 Permeases of the drug/metabolite transport... 110 3e-24 PAB2381 [GER] COG0697 Permeases of the drug/metabolite transport... 108 8e-24 PH0593 [GER] COG0697 Permeases of the drug/metabolite transporte... 99 1e-20 FN1038 [GER] COG0697 Permeases of the drug/metabolite transporte... 96 7e-20 AF0788 [GER] COG0697 Permeases of the drug/metabolite transporte... 96 9e-20 >CAC0076 [GER] COG0697 Permeases of the drug/metabolite transporter (DMT) superfamily Length = 303 Score = 110 bits (275), Expect = 3e-24, Method: Compositional matrix adjust. Identities = 90/302 (29%), Positives = 140/302 (46%), Gaps = 14/302 (4%) Query: 14 LLLVAMMWGCTFLIVQSAVKVLPPLAFNSIRFIGAALLLAPITAVFYRREWKMLSWRMAG 73 LL+ A++WG F +K + P FN IRFI A+ + P+ +F++ + + + A Sbjct: 12 LLITALIWGLAFTAQSVGMKYVGPFTFNGIRFILGAICIVPVM-LFFKEDKIEENSKYAR 70 Query: 74 HAXXXX----XXXXXXXXXQTMGLLYTTTSNTGFITGLSVVLVPFLSLALLKTAISRYTW 129 A Q +GL+YTT GFITGL +V+VP + + LK + +W Sbjct: 71 AALVGGIICGMVNFLGTTLQQIGLMYTTVGKAGFITGLYIVIVPIIGI-FLKHHMGINSW 129 Query: 130 FSAGLAVAGLYLLTFTGSALSLNKGDALVLICAVAFALQVAYTGVYAPRYXXXXXXXXXX 189 A+ GLYLL TGS S+ G+ L L A FA+Q+ ++ + Sbjct: 130 IGVLFALVGLYLLCNTGS-FSVGYGETLELSGAFFFAVQIFIIDHFSKKANCYRLAFFQY 188 Query: 190 XCVGLLSAGASLLVDGPAPLAHSGTLILKPDVLWALLVSIGPTSAFAFWIQTACQKYTTP 249 G +S +L + A G I +L+ L S+G A+ +Q QK P Sbjct: 189 VTCGAVSLIIALFTEKITFNALYGAAI---PILYGGLGSVG----IAYTLQIIGQKNAKP 241 Query: 250 SRVAVIFATEPVFAALTGLVFGGETLGVSALLGCLCILAAMLMAELSPGPAPAAQKDEEP 309 S A+I + E VF AL G + GE +G L+GC +L ML+A++ Q E+ Sbjct: 242 SHAAIIMSMESVFGALGGAIILGERMGAKNLIGCGLMLLGMLVAQIKLTKTSKKQIIEKK 301 Query: 310 SL 311 ++ Sbjct: 302 AV 303 >PAB2381 [GER] COG0697 Permeases of the drug/metabolite transporter (DMT) superfamily Length = 280 Score = 108 bits (271), Expect = 8e-24, Method: Compositional matrix adjust. Identities = 82/287 (28%), Positives = 138/287 (48%), Gaps = 19/287 (6%) Query: 4 VKRSRIAGLSLLLVAMMWGCTFLIVQSAVKVLPPLAFNSIRFIGAALLLAPITAVFYRRE 63 + +R A L L+L++ +WG TF +++ ++ PP+ F + RF+ A+LL+ +F R++ Sbjct: 1 MDETRKAELILILISAIWGSTFPVMKLGIEDYPPITFVAFRFLIASLLML----IFLRKD 56 Query: 64 WKMLSWRMAGHAXXXXXXXXXXXXXQTMGLLYTTTSNTGFITGLSVVLVPFLSLALLKTA 123 K A Q +GL YTT+SN+ FIT L +V PF+++ LLKT Sbjct: 57 IKT---SQALPGLLVGLSLFLGFSFQVVGLKYTTSSNSAFITSLYMVFTPFVAILLLKTR 113 Query: 124 ISRYTWFSAGLAVAGLYLLTFTGSALSLNKGDALVLICAVAFALQVAYTGVYAPRYXXXX 183 + W + GLA+ G YL++ G + N GD L ++ A++FA Q+ + Sbjct: 114 VKLIDWVALGLALLGTYLISNVG---NFNYGDMLTVLAALSFAFQIVLVEYFGN--LGIG 168 Query: 184 XXXXXXXCVGLLSAGASLLVDGPAPLAHSGTLILKPDVLWALLVSIGPTSAFAFWIQTAC 243 + S + + +G P+ G+ L+A++ + +A AF +Q Sbjct: 169 LAFWQVFWNSMFSLAYAAIFEG-LPMPREGS------TLFAIIYTAIMATAIAFAVQVKY 221 Query: 244 QKYTTPSRVAVIFATEPVFAALTGLVFGGETLGVSALLGCLCILAAM 290 Q R A+I++ EPVF + GE L +G L IL A+ Sbjct: 222 QPKIDSHRAAIIYSAEPVFGHFFSFLILGEVLSWKGYIGALLILIAV 268 >PH0593 [GER] COG0697 Permeases of the drug/metabolite transporter (DMT) superfamily Length = 284 Score = 98.6 bits (244), Expect = 1e-20, Method: Compositional matrix adjust. Identities = 83/290 (28%), Positives = 141/290 (48%), Gaps = 18/290 (6%) Query: 1 MTSVKRSRIAGLSLLLVAMMWGCTFLIVQSAVKVLPPLAFNSIRFIGAALLLAPITAVFY 60 M + +R A L LL ++ +WG TF +++ +K PP++F + RF A+++LA + Sbjct: 1 MKLMDETRKAELILLGLSAIWGTTFPVMKIGIKDFPPVSFIAFRFFLASIILAIVLKDKI 60 Query: 61 RREWKMLSWRMAGHAXXXXXXXXXXXXXQTMGLLYTTTSNTGFITGLSVVLVPFLSLALL 120 ++ ++ + G + Q +GL YTT SN+ FIT L +V PF++ ALL Sbjct: 61 TKD-QIFPGFLIGLSLFAGFGF------QVVGLKYTTASNSAFITSLYMVFTPFVAFALL 113 Query: 121 KTAISRYTWFSAGLAVAGLYLLTFTGSALSLNKGDALVLICAVAFALQVAYTGVYAPRYX 180 K+ I+R + +AV G YL+ +G++L+L+ GD L ++ A++FA Q+ Y + Sbjct: 114 KSKITRVDALALAIAVLGTYLI--SGASLNLSYGDLLTILAALSFAFQIVLIEYY--KDL 169 Query: 181 XXXXXXXXXXCVGLLSAGASLLVDGPAPLAHSGTLILKPDVLWALLVSIGPTSAFAFWIQ 240 + S S+ + PL G+ + +A+L + +A AF Q Sbjct: 170 GLGLAFWQIFWNFIFSLVYSIYFES-LPLPKEGSTV------FAILYTGIVATALAFLGQ 222 Query: 241 TACQKYTTPSRVAVIFATEPVFAALTGLVFGGETLGVSALLGCLCILAAM 290 Q R AV+++ EPVF + GE L +G IL A+ Sbjct: 223 VKYQPKIESHRAAVLYSAEPVFGYFFSFLILGEILPFEGYVGAFLILVAI 272 >FN1038 [GER] COG0697 Permeases of the drug/metabolite transporter (DMT) superfamily Length = 303 Score = 95.9 bits (237), Expect = 7e-20, Method: Compositional matrix adjust. Identities = 76/292 (26%), Positives = 125/292 (42%), Gaps = 7/292 (2%) Query: 5 KRSRIAGLSLLLVAMMWGCTFLIVQSAVKVLPPLAFNSIRFIGAALLLAPITAVFYRREW 64 K+ L L L A +WG F+ + + + P FN R I A + L + + Sbjct: 4 KKRYFGDLMLFLAAFIWGTAFVAQVTGMDRIGPFTFNMARSIIAIICLGAYLIITKSKLP 63 Query: 65 KMLSWRMAGHAXXXXXXXXXXXXXQTMGLLYTTTSNTGFITGLSVVLVPFLSLALLKTAI 124 K + + G Q +GL YTT TGFIT ++++PFL++ LK I Sbjct: 64 KDIGVLLQG-GLVCGFFIFMGTSLQQIGLQYTTAGKTGFITSFYILIIPFLTMIFLKHKI 122 Query: 125 SRYTWFSAGLAVAGLYLLTFTG-SALSLNKGDALVLICAVAFALQVAYTGVYAPRYXXXX 183 TW S + GLYLL S ++N+GD +V + + + + Y+ + Sbjct: 123 DLLTWISIIIGFIGLYLLAIPNLSDFTINRGDFIVFLGSFCWGGHILIIDYYSKKVSPVQ 182 Query: 184 XXXXXXXCVGLLSAGASLLVDGPAPLAHSGTLILKPDVLWALLVSIGPTSAFAFWIQTAC 243 + +LS +LL + ++ L K L +S A+ +Q Sbjct: 183 LSFLQFVMLTILSGICALLFENETATMNNIFLSWKSIAYAGFL-----SSGIAYTLQMVG 237 Query: 244 QKYTTPSRVAVIFATEPVFAALTGLVFGGETLGVSALLGCLCILAAMLMAEL 295 QKYT P ++I + E VFAAL G + E + LGC + A++ +++ Sbjct: 238 QKYTNPIIASLILSLEAVFAALAGYIMLDEVMTSREFLGCSIVFLAIIFSQI 289 >AF0788 [GER] COG0697 Permeases of the drug/metabolite transporter (DMT) superfamily Length = 308 Score = 95.5 bits (236), Expect = 9e-20, Method: Compositional matrix adjust. Identities = 89/293 (30%), Positives = 127/293 (43%), Gaps = 25/293 (8%) Query: 4 VKRSRIAGLSLLLVAMMWGCTFLIVQSAVKVLPPLAFNSIRFIGAALLLAPITAVFYRRE 63 VKR A L L LVA++WG TF +V+ A+ + P AFN++RF A L P + + Sbjct: 38 VKR-LYADLGLALVALIWGSTFPVVKIALDSMSPFAFNTVRFFIACLFFLP-----FLKG 91 Query: 64 WKMLSWRMAGHAXXXXXXXXXXXXXQTMGLLYTTTSNTGFITGLSVVLVPFLSLALLKTA 123 W G A QT+GL YTT +N GFIT VVL P +S + K Sbjct: 92 WDFKDGFKIGIA------SFLGYTFQTVGLDYTTATNAGFITSTYVVLAPIISWLVYKDV 145 Query: 124 ISRYTWFSAGLAVAGLYLLT-FTGSALSLNKGDALVLICAVAFALQVAYTGVYAPRYXXX 182 + LA G Y L+ ++G N GD L+L CA+ F ++A Y+ Sbjct: 146 FDKRDVSGVLLAFVGFYFLSGYSG----FNIGDILMLFCALFFGAEIAMISHYSRLSNPT 201 Query: 183 XXXXXXXXCVGLLSAGASLLVDGPAPLAHSGTLILKPDVLWALLVSIGPTSAFAFWIQTA 242 + +LSA P + + + V+ LL++ + A +Q Sbjct: 202 MLAFWQSFAIFILSA--------PFAVFTTTKFEINTTVILCLLITAFFATFVAKMLQNW 253 Query: 243 CQKYTTPSRVAVIFATEPVFAALTGLVFGGETLGVSALLGCLCILAAMLMAEL 295 Q YT S AVI + E VFA L + E L G IL A+++ L Sbjct: 254 LQSYTKSSDAAVILSLEGVFAHLFSVAVLAEILTPVQYFGAFLILLAVIIVSL 306 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.325 0.137 0.415 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 19,187,721 Number of extensions: 652062 Number of successful extensions: 2218 Number of sequences better than 1.0e-05: 26 Number of HSP's gapped: 2163 Number of HSP's successfully gapped: 27 Length of query: 317 Length of database: 38,378,431 Length adjustment: 106 Effective length of query: 211 Effective length of database: 26,153,027 Effective search space: 5518288697 Effective search space used: 5518288697 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.0 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.6 bits) S2: 116 (49.3 bits)