BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0307.0003.p (336 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value BS_yhfJ [H] COG0095 Lipoate-protein ligase A 394 e-109 BH0683 [H] COG0095 Lipoate-protein ligase A 392 e-109 lin0931 [H] COG0095 Lipoate-protein ligase A 369 e-102 SA0884 [H] COG0095 Lipoate-protein ligase A 367 e-101 lin0758 [H] COG0095 Lipoate-protein ligase A 328 1e-89 >BS_yhfJ [H] COG0095 Lipoate-protein ligase A Length = 331 Score = 394 bits (1012), Expect = e-109, Method: Compositional matrix adjust. Identities = 194/334 (58%), Positives = 245/334 (73%), Gaps = 4/334 (1%) Query: 1 MLFIDNTGITDASINLAIEEFALKNLPMDDSYLLFYINSPSIIIGKHQNTIEEINQEYVK 60 MLFIDN I D INLAIEE+ +K+L + YLLFY+N PSIIIGK+QNTIEEIN +YV+ Sbjct: 1 MLFIDNQNINDPRINLAIEEYCVKHLDPEQQYLLFYVNQPSIIIGKNQNTIEEINTKYVE 60 Query: 61 DHNIQVVRRLSGGGAVYHDLGNLNFSFITKDDGQSFHNFLKFTQPVIDYLQSMGVNAELS 120 ++ I VVRRLSGGGAVYHDLGNLNFSFITKDDG SFHNF KFT+PVI L +GV AELS Sbjct: 61 ENGIIVVRRLSGGGAVYHDLGNLNFSFITKDDGDSFHNFKKFTEPVIQALHQLGVEAELS 120 Query: 121 GRNDXXXXQVGEQKISGNAQFSTRGRMFSHGTLMFDLNLDDVQASLNVNPEXXXXXXXXX 180 GRND V +KISGNAQF+T+GR+FSHGTLMFD +D V ++L V + Sbjct: 121 GRNDIV---VDGRKISGNAQFATKGRIFSHGTLMFDSAIDHVVSALKVKKDKIESKGIKS 177 Query: 181 XXXXXANIKDLLGKEMTIEEFRAGLLRSIFGM-EPSEVPQYKLTMDDWVRINEISREHYQ 239 ANI + L +MT EEFR+ LLR IF + VP+YKLT DW I++IS+E YQ Sbjct: 178 IRSRVANISEFLDDKMTTEEFRSHLLRHIFNTNDVGNVPEYKLTEKDWETIHQISKERYQ 237 Query: 240 NWDWNYGLSPKSNVKHTRKFPAGLVDIRMDIEDSYIRDIKIYGDFFGVGDVADVENALRG 299 NWDWNYG SPK N+ H++++P G +D+ ++++ I D KI+GDFFGVGDV+++EN L G Sbjct: 238 NWDWNYGRSPKFNLNHSKRYPVGSIDLHLEVKKGKIEDCKIFGDFFGVGDVSEIENLLVG 297 Query: 300 KRYENAEVREALAGLDLKHYFGRIEPEDFIGLIF 333 K+YE + + + L G++LKHYFG I EDF+ LI+ Sbjct: 298 KQYERSVIADVLEGVNLKHYFGNITKEDFLDLIY 331 >BH0683 [H] COG0095 Lipoate-protein ligase A Length = 330 Score = 392 bits (1007), Expect = e-109, Method: Compositional matrix adjust. Identities = 192/333 (57%), Positives = 249/333 (74%), Gaps = 3/333 (0%) Query: 1 MLFIDNTGITDASINLAIEEFALKNLPMDDSYLLFYINSPSIIIGKHQNTIEEINQEYVK 60 MLFIDN GITD INLAIEE+ALKNL ++++YLLFYIN PSIIIG++QNTIEEIN EYV+ Sbjct: 1 MLFIDNQGITDPRINLAIEEYALKNLDINETYLLFYINEPSIIIGRNQNTIEEINTEYVE 60 Query: 61 DHNIQVVRRLSGGGAVYHDLGNLNFSFITKDDGQSFHNFLKFTQPVIDYLQSMGVNAELS 120 + I VVRRLSGGGAVYHD GNLNFSFITKDDG+SF NF KFT PVI L +GV AEL Sbjct: 61 SNGIHVVRRLSGGGAVYHDHGNLNFSFITKDDGESFSNFQKFTDPVIKALAKLGVTAELK 120 Query: 121 GRNDXXXXQVGEQKISGNAQFSTRGRMFSHGTLMFDLNLDDVQASLNVNPEXXXXXXXXX 180 GRND +KISGNAQFST+GRMFSHGTL+FD +D V ++LNV+ + Sbjct: 121 GRNDIIASD--GRKISGNAQFSTKGRMFSHGTLLFDSEIDHVVSALNVSKDKIESKGIKS 178 Query: 181 XXXXXANIKDLLGKEMTIEEFRAGLLRSIFGMEPSEVPQYKLTMDDWVRINEISREHYQN 240 ANI + L ++++I++FR+ LL SIF + + + +YKLT DDW I+E+S+E YQN Sbjct: 179 IRSRVANISEFLTEKISIDQFRSLLLESIFDGQAN-IQEYKLTADDWAEIHELSKERYQN 237 Query: 241 WDWNYGLSPKSNVKHTRKFPAGLVDIRMDIEDSYIRDIKIYGDFFGVGDVADVENALRGK 300 WDWNYG SP N++H+ +FP G +DIR++++ I+ KI+GDFFG GDV D+E+ L G Sbjct: 238 WDWNYGKSPAFNLQHSHRFPVGNIDIRLEVKGGTIQQCKIFGDFFGTGDVRDLEDRLVGI 297 Query: 301 RYENAEVREALAGLDLKHYFGRIEPEDFIGLIF 333 RYE A++ +ALA +D+K YFG++E +D + L + Sbjct: 298 RYERADIEQALADVDVKTYFGQVEKDDILNLFY 330 >lin0931 [H] COG0095 Lipoate-protein ligase A Length = 671 Score = 369 bits (947), Expect = e-102, Method: Compositional matrix adjust. Identities = 20/334 (5%), Positives = 54/334 (16%), Gaps = 4/334 (1%) Query: 1 MLFIDNTGITDASINLAIEEFALKNLPMDDSYLLFYINSPSIIIGKHQNTIEEINQEYVK 60 M I+ + E + + + + ++ + Sbjct: 1 MESIEQQLTELRTTLRHHEYLYHVMDAPEIPDAEYDRLMRELRELETKHPELITPDSPTQ 60 Query: 61 DHNIQVVRRLSGGGAVYHDLGNLNFSFITKDDGQSFHNFLKFTQPVIDYLQSMGVNAELS 120 + S L N + + L+ Sbjct: 61 RVGAAPLAAFSQIRHEVPMLSLDNVFDEESFLAFNKRVQDRLKNNEKVTWCCELKLDGLA 120 Query: 121 GRNDXXXXQVGEQKISGNAQFSTRGRMFSHGTLMFDLNLDDVQASLNVNPEXXXXXXXXX 180 G + T G + + Sbjct: 121 VSILYE---NGVLVSAATRGDGTTGEDITSNVRTIRAIPLKLHGENIPARLEVRGEVFLP 177 Query: 181 XXXXXANIKDLLGKEMTIEEFRAGLLRSIFGMEPSE-VPQYKLTMDDWVRINEISREHYQ 239 +D + + LT + E Sbjct: 178 QAGFEKINEDARRTGGKVFANPRNAAAGSLRQLDPRITAKRPLTFFCYGVGVLEGGELPD 237 Query: 240 NWDWNYGLSPKSNVKHTRKFPAGLVDIRMDIEDSYIRDIKIYGDFFGVGDVADVENALRG 299 K + + + + + + + F G V V + + Sbjct: 238 THLGRLLQFKKWGLPVSDRVTLCESAEEVLAFYHKVEEDRPTLGFDIDGVVIKVNSLAQQ 297 Query: 300 KRYENAEVREALAGLDLKHYFGRIEPEDFIGLIF 333 ++ A ++ + Sbjct: 298 EQLGFVARAPRWAVAFKFPAQEQMTFVRDVEFQV 331 >SA0884 [H] COG0095 Lipoate-protein ligase A Length = 328 Score = 367 bits (943), Expect = e-101, Method: Compositional matrix adjust. Identities = 182/332 (54%), Positives = 232/332 (69%), Gaps = 5/332 (1%) Query: 1 MLFIDNTGITDASINLAIEEFALKNLPMDDSYLLFYINSPSIIIGKHQNTIEEINQEYVK 60 M FI N ITD ++NLA+EE+ LKNLP ++SY LFYIN PSII+GK+QNTIEE+NQ Y+ Sbjct: 1 MKFISNNNITDPTLNLAMEEYVLKNLPAEESYFLFYINRPSIIVGKNQNTIEEVNQTYID 60 Query: 61 DHNIQVVRRLSGGGAVYHDLGNLNFSFITKDDGQSFHNFLKFTQPVIDYLQSMGVNAELS 120 HNI VVRR+SGGGAVYHD GNLNFSFIT DDG SFHNF KFT+P++ LQS+GVNAEL+ Sbjct: 61 AHNIDVVRRISGGGAVYHDTGNLNFSFITDDDGNSFHNFQKFTEPIVQALQSLGVNAELT 120 Query: 121 GRNDXXXXQVGEQKISGNAQFSTRGRMFSHGTLMFDLNLDDVQASLNVNPEXXXXXXXXX 180 GRND QVG+ KISGNA + RMFSHGTLM + +LD+VQ +L VNP Sbjct: 121 GRND---IQVGQAKISGNAMVKVKNRMFSHGTLMLNSDLDEVQNALKVNPAKIKSKGIKS 177 Query: 181 XXXXXANIKDLLGKEMTIEEFRAGLLRSIFGMEPSEVPQYKLTMDDWVRINEISREHYQN 240 ANI++ L + IEEF+ +L++IFG +EV +YKLT +DW I ++S + Y+ Sbjct: 178 VRKRVANIQEFLNDPLEIEEFKKIILKTIFG--ETEVEEYKLTDEDWENIEKLSNDKYRT 235 Query: 241 WDWNYGLSPKSNVKHTRKFPAGLVDIRMDIEDSYIRDIKIYGDFFGVGDVADVENALRGK 300 W WNYG +PK N + KF G V I+ D++ I KI+GDFFGVGDV D+ENAL G Sbjct: 236 WAWNYGRNPKYNFEREEKFEKGFVQIKFDVKRGKIEHAKIFGDFFGVGDVTDLENALVGC 295 Query: 301 RYENAEVREALAGLDLKHYFGRIEPEDFIGLI 332 ++ + EAL+ DL HYFG I+ + I L+ Sbjct: 296 LHDFEHIEEALSEYDLYHYFGDIDRHELIRLM 327 >lin0758 [H] COG0095 Lipoate-protein ligase A Length = 671 Score = 328 bits (840), Expect = 1e-89, Method: Compositional matrix adjust. Identities = 20/333 (6%), Positives = 54/333 (16%), Gaps = 5/333 (1%) Query: 1 MLFIDNTGITDASINLAIEEFALKNLPMDDSYLLFYINSPSIIIGKHQNTIEEINQEYVK 60 M I+ + E + + + + ++ + Sbjct: 1 MESIEQQLTELRTTLRHHEYLYHVMDAPEIPDAEYDRLMRELRELETKHPELITPDSPTQ 60 Query: 61 DHNIQVVRRLSGGGAVYHDLGNLNFSFITKDDGQSFHNFLKFTQPVIDYLQSMGVNAELS 120 + S L N + + L+ Sbjct: 61 RVGAAPLAAFSQIRHEVPMLSLDNVFDEESFLAFNKRVQDRLKNNEKVTWCCELKLDGLA 120 Query: 121 GRNDXXXXQVGEQKISGNAQFSTRGRMFSHGTLMFDLNLDDVQASLNVNPEXXXXXXXXX 180 + G + T G + + Sbjct: 121 VSIL---YENGVLVSAATRGDGTTGEDITSNVRTIRAIPLKLHGENIPARLEVRGEVFLP 177 Query: 181 XXXXXANIKDLLGKEMTIEEFRAGLLRSIFGMEPSEVPQYKLTMDDWVRINEISREHYQN 240 +D + + + E + Sbjct: 178 QAGFEKINEDARRTGGKVFANPRNAAAGSL--RQLDPRITAKRPLTFFCYGVGVLEGGEL 235 Query: 241 WDWNYGLSPKSNVKHTRKFPAGLVDIRMDIEDSYIRDIKIYGDFFGVGDVADVENALRGK 300 D + G + + + ++ ++ G V Sbjct: 236 PDTHLGRLLQFKKWGLPVSDRVTLCESAEEVLAFYHKVEEDRPTLGFDIDGVVIKVNSLA 295 Query: 301 RYENAEVREALAGLDLKHYFGRIEPEDFIGLIF 333 + E + F E F+ + Sbjct: 296 QQEQLGFVARAPRWAVAFKFPAQEQMTFVRDVE 328 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.319 0.139 0.407 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 24,103,977 Number of extensions: 1001513 Number of successful extensions: 1506 Number of sequences better than 1.0e-05: 23 Number of HSP's gapped: 1467 Number of HSP's successfully gapped: 23 Length of query: 336 Length of database: 38,378,431 Length adjustment: 107 Effective length of query: 229 Effective length of database: 26,037,693 Effective search space: 5962631697 Effective search space used: 5962631697 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 116 (49.3 bits)