BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0305.0033.p (438 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value BS_yurO [G] COG1653 ABC-type sugar transport system, periplasmic... 144 3e-34 SMa2305 [G] COG1653 ABC-type sugar transport system, periplasmic... 78 3e-14 PH1214 [G] COG1653 ABC-type sugar transport system, periplasmic ... 76 1e-13 BMEII0542 [G] COG1653 ABC-type sugar transport system, periplasm... 74 3e-13 VCA1033 [G] COG1653 ABC-type sugar transport system, periplasmic... 66 1e-10 >BS_yurO [G] COG1653 ABC-type sugar transport system, periplasmic component Length = 422 Score = 144 bits (363), Expect = 3e-34, Method: Compositional matrix adjust. Identities = 118/404 (29%), Positives = 190/404 (47%), Gaps = 28/404 (6%) Query: 46 VTLNMMHPWTSPNVDNEVYKARIAK-FEEEHPNIVIKQDGVPSAQYKTKLRTLAAANNLA 104 VTL H W P + Y + K FE++HP+I I+ + V + YK K++ + + Sbjct: 32 VTLKFFHRW--PKEPEKSYFEEVVKEFEKDHPDIDIQIEAVLNDSYKDKIKVMLGTTSPP 89 Query: 105 DINVVWPGADLEPLVEGSLVEPIDSLMDN---WKAILPETALAGFNIGGKQYAIPTKQTF 161 DI W + G+ + S N W + L ++ + F KQY +P Q Sbjct: 90 DIYFSWSDEFAFKFIRGNKALDLSSYYKNDTDWSSQLVQSQITPFTYENKQYGVPW-QMD 148 Query: 162 VDIIYYNKDMFAQVGYTEFPDTYDKLIDAVKKLKAAGITPISLGNKEQWPLQSSYLSIIG 221 +YNKD+F ++ + P T+D+LID KKLK G TPIS G K W + S Y+ + Sbjct: 149 AKSFFYNKDIFQKLN-LDPPKTWDELIDVSKKLKEHGYTPISFGTKATWTI-SHYIGTLN 206 Query: 222 DRFTGSDFLSK-VMKKEAKFTDPDFVKAISVIDELTKLDAFNTDANNMDSVQGQDYFIQG 280 R K K +FTD +VKA+ + EL + FN N++D + F G Sbjct: 207 QRMVDEKTREKDYNAKTGEFTDEGYVKALEKLQEL--MPYFNKHVNSVDHEYVRQQFKSG 264 Query: 281 KAAMHISSSTVDARIRINNDEGAKFGIALFPSVNGGKGDPVKSAGVVQYGIAIKSGLDEK 340 K+AM I + T + ++ E G+ FP ++G KG G + G I S + Sbjct: 265 KSAM-IYAETAEIKLV----EPVNLGMFPFPEISGQKGSSEALTGAPE-GFMISS----R 314 Query: 341 TKAAAE--EFMKYFVSEDLYKELIRNGVVVPAT--VEIPEDASPYLKEMLE--LTSKGTA 394 TK E EF+++ S+ + ++L+++ A E+A+ +E ++ + +K Sbjct: 315 TKHPKEAMEFLQFLTSKRMGEKLVKDVGKYSAVQGTATEENATAIQREAVQHIVDAKSMV 374 Query: 395 PVFDSIIPTQVVDVVQNGIQALTVGRGTPEQVAKDAQEAVDQLN 438 P FD + +V D G+Q + G TP+QV K Q+A Q+ Sbjct: 375 PWFDMDVDVEVADAYLTGVQQMLGGDMTPQQVMKAVQKAAKQVR 418 >SMa2305 [G] COG1653 ABC-type sugar transport system, periplasmic component Length = 459 Score = 77.8 bits (190), Expect = 3e-14, Method: Compositional matrix adjust. Identities = 80/340 (23%), Positives = 137/340 (40%), Gaps = 19/340 (5%) Query: 90 YKTKLRTLAAANNLADINVVWPGADLEPLVEGSLVEPIDSLMDNWKAILPETALAG-FNI 148 +KT +R A D+ + G + V L + I L + K A AG + Sbjct: 117 HKTAIRNYLVAG-APDVCFWFSGNRMRAFVTRGLFDDISDLFEKEKYADVLGATAGSVTV 175 Query: 149 GGKQYAIPTKQTFVDIIYYNKDMFAQVGYTEFPDTYDKLIDAVKKLKAAGITPISLGNKE 208 GKQY +PT T + +Y KD+F + G + P ++D L+ +K K+A +TP+++G K+ Sbjct: 176 EGKQYGLPTGGTLWGM-FYRKDVFDEHGL-KVPTSWDDLLAFGEKCKSASLTPVAMGTKD 233 Query: 209 QWPLQSSYLSIIGDRFTGSDFLSKVMKKEAKFTDPDFVKAISVIDELTKLDAFNTDANNM 268 WP + + + R G D +M E +TD +EL K F D + Sbjct: 234 LWP-AAGWFDQMNLRINGLDKHFALMNGEMAYTDETLKPVFEHWEELIKQGFFTPDHTSF 292 Query: 269 DSVQGQDYFIQGKAAMHISSSTVDARIRINNDEGAKFGIALFPSVNGGKGDPVKSAGVVQ 328 + + Q +A M + V R + + A FP + G G + Sbjct: 293 GWQEAGAFLAQKRAGMMNLGAFV--RAAFPRQDLPQLTFAPFPVIEDGLGR------YEE 344 Query: 329 YGI-AIKSGLDEKTKAAAEEFMKYFVSEDLYKELIRNGVVVPATVEIPEDASPYLKEMLE 387 + + ++ + K K A EF+ +F + + G VP ++P P + +E Sbjct: 345 FSLNSVHIPTNAKNKPGAREFLTHFYKPENLAAYLEPGGNVPPRNDLPPSKDPLVNAAVE 404 Query: 388 LTS--KGTAPVFDSIIPTQVVDVVQNGIQALTVGRGTPEQ 425 GT+ +D D+ Q G+ PE+ Sbjct: 405 SLKAVAGTSQFYDRDTDP---DMAQAGLVGFQEFMARPER 441 >PH1214 [G] COG1653 ABC-type sugar transport system, periplasmic component Length = 441 Score = 75.9 bits (185), Expect = 1e-13, Method: Compositional matrix adjust. Identities = 63/247 (25%), Positives = 108/247 (43%), Gaps = 9/247 (3%) Query: 43 EETVTLNMMHPWTSPNVDNEVYKARIAKFEEEHPNIVIKQDGVPSA---QYKTKLRTLAA 99 ++ L + H WT+ + E + A KFE+++PNI IK + V K L++L Sbjct: 39 QQVQELEIYHWWTAGG-EKEAFDALAKKFEKKYPNIKIKANPVSGGGGVNLKMVLQSLML 97 Query: 100 ANNLADINVVWPGADLEPLVEGSLVEPIDSLM-DNWKAILPETALAGFNIGGKQYAIPTK 158 A D V G ++ ++ + + PID + ++ K P+ YA+P Sbjct: 98 AGKPPDTFQVHAGYEMARYIKANQLTPIDDIWTEDMKKNYPKVIQQMVIFNNHYYAVPIN 157 Query: 159 QTFVDIIYYNKDMFAQVGYTEFP-DTYDKLIDAVKKLKAAGITPISLGNKEQWPLQSSYL 217 ++I+YNK +F + G T D L +KLK G+TP +LG++ +WP + Sbjct: 158 VHRANVIWYNKQIFKKYGIDPSSIKTIDDLFAVAEKLKENGVTPFALGDRNKWP-ATQVF 216 Query: 218 SIIGDRFTGSDFLSKVMKKEAKFTDPDFVKAISVIDELTKLDAFNTDANNMDSVQGQDYF 277 ++ G D+ K + E DP K + + + + A D Y Sbjct: 217 EVMLVAVGGVDYYEKFINGEISANDPTLKKVLEYFVKYVEYSNPDHAAKTWDEACALVY- 275 Query: 278 IQGKAAM 284 +G+AAM Sbjct: 276 -KGEAAM 281 >BMEII0542 [G] COG1653 ABC-type sugar transport system, periplasmic component Length = 398 Score = 74.3 bits (181), Expect = 3e-13, Method: Compositional matrix adjust. Identities = 87/366 (23%), Positives = 157/366 (42%), Gaps = 28/366 (7%) Query: 71 FEEEHPNIVIKQDGVPSAQYKTKLRTLAAANNLADINVV---WPGADLEPLVEGSLVEPI 127 FE E+P I + + VP KL T +A AD++++ W L V+ + EP+ Sbjct: 37 FEAENPGITLNFEVVPWDVLLQKLTTDISAGTNADLSIIGTRW----LIDFVQQGIAEPL 92 Query: 128 DSLM-DNWKAILPETALAGFNIGGKQYAIPTKQTFVDIIYYNKDMFAQVGYTEFPDTYDK 186 D M D +K ET L+ + GK Y +P + V +YYNKD+F + G P +D+ Sbjct: 93 DGYMNDEFKGRFIETFLSPSVLDGKTYGLPIAAS-VRAMYYNKDLFEKAGIKNPPANWDE 151 Query: 187 LIDAVKKLKAAGITPISLGNKEQWPLQSSYLSIIGDRFTGSDFLSKVMKKEAKFTDPDFV 246 L K+KA G G + + +++ G++ L+K + + P + Sbjct: 152 LKADAAKIKALGGENYGFGLQGK-EIETDVYYYYAMWSYGTEILNK--DGTSGLSTPGAL 208 Query: 247 KAISVIDELTKLDAFNTDANNMDSVQGQDYFIQGKAAMHISSSTVDARIRINNDEGAKFG 306 +A + + + Q+ F QGK M I++ + +I+ K+G Sbjct: 209 EAAKLYKSMIDDGLTQPGVTSYAREDVQNLFKQGKVGMMITAPFLSNQIK-EEAPNLKYG 267 Query: 307 IALFPS-VNGGKGDPVKSAGVVQYGIAIKSGLDEKTKAAAEEFMKYFVSEDLYKELIRNG 365 +A P+ G +G + GV I + + K K A + + + +D + +N Sbjct: 268 VAAIPAGPTGARG----TYGVTDSVIMFQ---NSKNKEEAWKVLDFLFQKDWRAKFTQNE 320 Query: 366 VVVPATVEIPEDASPYL---KEMLELTSKGTAPVFDSIIP--TQVVDVVQNGIQALTVGR 420 +P E E Y ++ T+ F +IP ++ D+ N +Q++ +G+ Sbjct: 321 GFLPVNKE--EAKMDYYVNNADLAAFTALLPDARFAPVIPGWEEIADITSNAMQSIYLGK 378 Query: 421 GTPEQV 426 G P+ V Sbjct: 379 GEPDAV 384 >VCA1033 [G] COG1653 ABC-type sugar transport system, periplasmic component Length = 458 Score = 66.2 bits (160), Expect = 1e-10, Method: Compositional matrix adjust. Identities = 88/388 (22%), Positives = 158/388 (40%), Gaps = 42/388 (10%) Query: 68 IAKFEEEHPNIVIKQDGVPSAQYKTKLRTLAAANNLADINVVWPGADLEPLVEGSLVEPI 127 I FE +P I +K + VP+ Y L A D+ P D L + + I Sbjct: 40 IPAFEAANPGIKVKFNPVPNVNYTPTLWENLKAGKAGDLITCRPFDDSLALFKAGHLAEI 99 Query: 128 DSL--MDNWKAILPETALAGFNI--GGKQYAIPTKQTFVDIIYYNKDMFAQVGYTEFPDT 183 + M+N P A A + G + + +P + + +YNK +F ++G + P T Sbjct: 100 TEMAGMEN----FPSFAQAPWQTDSGAQTFCVPMA-SVIHGFFYNKKIFNELGLS-VPQT 153 Query: 184 YDKLIDAVKKLKAAG-ITPISLGNKEQW-PLQSSYLSIIGDRFTGSDFLSKVMKKEAKFT 241 ++ + K+KA G P+S+ E W + Y +I + + G D ++ + Sbjct: 154 REQFFTVLDKVKADGRYIPLSMSGSESWVASELGYQNIGPNYWKGEDGRLALINGQEHLD 213 Query: 242 DPDFVKAISVIDELTKLDAF-NTDANNMDSVQGQDYFIQGKAAMHISSSTVDARIRINND 300 D +VK V +EL + A+ D D + F GKAA++++ S A D Sbjct: 214 DSQYVK---VFEELARWRAYLGEDGELRDYGTSNELFTSGKAALYVAGSWEIAPFTDKVD 270 Query: 301 EGAKFGIALFPSVNGGKGDPVKSAGVVQYGIAIKSGLDEKTKAAAEEFMKYFVSEDLYKE 360 FG+ P G G + G+ S K AA F+++ + + + E Sbjct: 271 ----FGVMRPPVAKQGDGCFFTDHTDIGMGMNPAS----KNPQAAMAFLQWLTTPE-FAE 321 Query: 361 LIRNGVVVPATVEIPED----ASPYLKEMLELTSKGTAPVFDSIIPTQVVDVVQNGI--- 413 L N +P + +P +EM+E + + + + TQ++ Q + Sbjct: 322 LYTNS--LPGFFSLSNHFFDVTNPAAREMMEWRDQCDSTI---RVATQILSRGQPKLGDE 376 Query: 414 -----QALTVGRGTPEQVAKDAQEAVDQ 436 QA+ VG+ TP A+ ++ + + Sbjct: 377 LAEVSQAVLVGKMTPTAAAERLEQGLKR 404 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.314 0.133 0.375 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 30,547,506 Number of extensions: 1304041 Number of successful extensions: 3183 Number of sequences better than 1.0e-05: 28 Number of HSP's gapped: 3187 Number of HSP's successfully gapped: 28 Length of query: 438 Length of database: 38,378,431 Length adjustment: 109 Effective length of query: 329 Effective length of database: 25,807,025 Effective search space: 8490511225 Effective search space used: 8490511225 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (22.0 bits) S2: 117 (49.7 bits)