BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0305.0030.p (459 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value alr1793 [G] COG0726 Predicted xylanase/chitin deacetylase 109 9e-24 BH1917 [G] COG0726 Predicted xylanase/chitin deacetylase 105 1e-22 DR1077_1 [G] COG0726 Predicted xylanase/chitin deacetylase 104 3e-22 SPy1370_2 [G] COG0726 Predicted xylanase/chitin deacetylase 102 1e-21 all4345 [G] COG0726 Predicted xylanase/chitin deacetylase 100 4e-21 >alr1793 [G] COG0726 Predicted xylanase/chitin deacetylase Length = 359 Score = 109 bits (273), Expect = 9e-24, Method: Compositional matrix adjust. Identities = 8/212 (3%), Positives = 25/212 (11%), Gaps = 16/212 (7%) Query: 27 GYRVFRKGIGRSE--FALTFDDGPDSLYTPXXXXXXKRYGAKATFFVVGSHAEQNPEIIK 84 + + + SL P G + + Sbjct: 87 LPTISAQHFHTAVHNEEQLAALEEASLDEPVTVWMKLDTGMHRLGVRPEQAEAFYHRLTQ 146 Query: 85 RMHDEGHLIGIHNYVHKTNWL--MRPATVKRQIQHTDDIIFSITGERSTYYRPPWGIVN- 141 + + + ++ AT K+ Sbjct: 147 CKNVRQPVNIVSHFAR---ADEPKCGATEKQLAIFNTFCEGKPGQRSIAASGGILLWPQS 203 Query: 142 -LFDFSKRRQVQIVLWSAMFGDWKEKLGADRLTEKLLAKLNPGEVMLLHDCGTTLGADPN 200 + V + ++ Sbjct: 204 HFDWVRPGIILYGVSPLEDRSTGADFGCQPVMSLTSSLIAVREHKAGEPVGY-------G 256 Query: 201 APEHMLVALERMLQEAEGRGLRSIRIDEMIRM 232 + + Sbjct: 257 GTWVSERDTRLGVVAMGYGDGYPRAAPSGTPV 288 >BH1917 [G] COG0726 Predicted xylanase/chitin deacetylase Length = 276 Score = 105 bits (263), Expect = 1e-22, Method: Compositional matrix adjust. Identities = 61/197 (30%), Positives = 106/197 (53%), Gaps = 10/197 (5%) Query: 38 SEFALTFDDGPDSLYTPXXXXXXKRYGAKATFFVVGSHAEQNPEIIKRMHDEGHLIGIHN 97 + ALTFDDGPD +TP + Y ATFFV+G AE+ P+++ RM EGH+IG H+ Sbjct: 80 NRIALTFDDGPDPRFTPQVLDVLQEYNVPATFFVMGRRAERFPDLVTRMAAEGHVIGNHS 139 Query: 98 YVHKTNWLMRP--ATVKRQIQHTDDIIFSITGERSTYYRPPWGIV--NLFDFSKRRQVQI 153 Y H N+++ A +++++ T+ II ++ G R ++R P+G + L + + Sbjct: 140 YSHP-NFVVEDDVALLEQEVLQTEAIIQNLVGYRPRFFRAPYGFLYNELVEKLAELNNTV 198 Query: 154 VLWSAMFGDWKEKLGADRLTEKLLAKLNPGEVMLLHDCGTTLGADPNAPEHMLVALERML 213 V WS DW+E+ A+ + +++ PG ++L+HD G E AL +++ Sbjct: 199 VAWSIDTLDWRER-PAEEVAFSVISSAQPGSIVLMHDGAEADGDRTQTIE----ALRQII 253 Query: 214 QEAEGRGLRSIRIDEMI 230 + +G + + E+I Sbjct: 254 PTLQEQGFEFVTVSELI 270 >DR1077_1 [G] COG0726 Predicted xylanase/chitin deacetylase Length = 225 Score = 104 bits (260), Expect = 3e-22, Method: Compositional matrix adjust. Identities = 69/225 (30%), Positives = 107/225 (47%), Gaps = 10/225 (4%) Query: 8 LFYISTFYAFIPGMISRIFGYRVFRKGI-GRSEFALTFDDGPDSLYTPXXXXXXKRYGAK 66 L + Y +P ++ ++ + R+G R E ALTFDDGPD TP + Sbjct: 8 LLLGAALYIGLPYLLVQVGNLGLVREGRRARREVALTFDDGPDPQTTPAVLAALREADMH 67 Query: 67 ATFFVVGSHAEQNPEIIKRMHDEGHLIGIHNYVHKTNWLMRPATVKRQIQHTDDIIFSIT 126 ATFFV+ A+ +P++I++M +EGH + H H W+ P + ++T Sbjct: 68 ATFFVIAGKAQAHPDLIRQMLEEGHEVEAHADKHVHAWIRTPWGAALDPLRAVRAVGAMT 127 Query: 127 GERSTYYRPPWGIVNLFDFSKRR--QVQIVLWSAMFGDWKEKLGADRLTEKLLAKLNPGE 184 G ++RPP G L + +R V+ WS DW + D + E+L A L PG Sbjct: 128 GRPVRFHRPPHGAYTLSTWLGQRLAGVRGAHWSIEGCDWHPESIPDTVRERLAALLVPGA 187 Query: 185 VMLLHDCGTTLGADPNAPEHMLVALERMLQEAEGRGLRSIRIDEM 229 V++LHD G GA P L +L + + RG RS+ + E+ Sbjct: 188 VIVLHDAGP--GARVTVP-----LLPSLLADLKARGYRSVTLAEL 225 >SPy1370_2 [G] COG0726 Predicted xylanase/chitin deacetylase Length = 223 Score = 102 bits (255), Expect = 1e-21, Method: Compositional matrix adjust. Identities = 58/180 (32%), Positives = 89/180 (49%), Gaps = 3/180 (1%) Query: 27 GYRVFRKGIGRSEFALTFDDGPDSLYTPXXXXXXKRYGAKATFFVVGSHAEQNPEIIKRM 86 YR +++ + ALTFDDGPD TP +Y AK TFF++GS N + KR+ Sbjct: 29 NYRTYKEQHPKKLVALTFDDGPDPTTTPQVLDILAKYQAKGTFFMIGSKVVNNENLTKRV 88 Query: 87 HDEGHLIGIHNYVHKTNWLMRPATVKRQIQHTDDIIFSITGERSTYYRPPWGIVNLFDFS 146 D GH I H + H + + ++ Q+ T+ I G++ Y RPP+G N Sbjct: 89 SDAGHEIANHTWDHPNLTNLSVSEIQHQVNMTNQAIEKACGKKPRYLRPPYGATNAT-VQ 147 Query: 147 KRRQVQIVLWSAMFGDWKEKLGADRLTEKLLAKLNPGEVMLLHDCG-TTLGADPNAPEHM 205 + + +LW+ DW E D + + +L PG V+L+HD TT+ A P E++ Sbjct: 148 QSSGLTQMLWTVDTRDW-ENHSTDGIMTNVKNQLQPGGVVLMHDIHQTTINALPTVMEYL 206 >all4345 [G] COG0726 Predicted xylanase/chitin deacetylase Length = 305 Score = 100 bits (250), Expect = 4e-21, Method: Compositional matrix adjust. Identities = 58/201 (28%), Positives = 101/201 (50%), Gaps = 15/201 (7%) Query: 36 GRSEFALTFDDGPDSLYTPXXXXXXKRYGAKATFFVVGSHAEQNPEIIKRMHDEGHLIGI 95 G+ ALTFDDGP T K+ KATFFVVG + + P++++R+ EGH IG Sbjct: 104 GQKVIALTFDDGPWPESTTQVLDILKQNNIKATFFVVGQNVKNYPDLLRRVAAEGHAIGN 163 Query: 96 HNYVHKTNW--LMRPATVKRQIQHTDDIIFSITGERSTYYRPPWGIVN--LFDFSKRRQV 151 H + H W M P ++ +T ++I+ ITG ++ +RPP G++N L +++ + Sbjct: 164 HTWHH---WYHFMNPQVAAYEVNNTTNLIYQITGVKTDLFRPPGGMMNNGLVSYARNNKY 220 Query: 152 QIVLWSAMFGDWKEKLGADRLTEKLLAKLNPGEVMLLHDCGTTLGADPNAPEHMLVALER 211 +++WS+ D+ + RL + PG ++L+HD G + AL Sbjct: 221 AVIMWSSDSIDY-SRPSVPRLINNVFRLAKPGGIVLMHDGGGNR-------TQTVQALPE 272 Query: 212 MLQEAEGRGLRSIRIDEMIRM 232 ++ +G + + E++ M Sbjct: 273 IIARFRKQGYSFVTVPELLEM 293 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.324 0.139 0.419 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 33,759,839 Number of extensions: 1384663 Number of successful extensions: 4224 Number of sequences better than 1.0e-05: 35 Number of HSP's gapped: 4195 Number of HSP's successfully gapped: 35 Length of query: 459 Length of database: 38,378,431 Length adjustment: 110 Effective length of query: 349 Effective length of database: 25,691,691 Effective search space: 8966400159 Effective search space used: 8966400159 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.0 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (22.0 bits) S2: 117 (49.7 bits)