BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0305.0027.p (694 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value BS_mcpC [NT] COG0840 Methyl-accepting chemotaxis protein 112 2e-24 BH2275 [NT] COG0840 Methyl-accepting chemotaxis protein 108 3e-23 BS_tlpA [NT] COG0840 Methyl-accepting chemotaxis protein 105 3e-22 BS_mcpB [NT] COG0840 Methyl-accepting chemotaxis protein 94 9e-19 BS_mcpA [NT] COG0840 Methyl-accepting chemotaxis protein 92 3e-18 >BS_mcpC [NT] COG0840 Methyl-accepting chemotaxis protein Length = 654 Score = 112 bits (280), Expect = 2e-24, Method: Compositional matrix adjust. Identities = 93/374 (24%), Positives = 174/374 (46%), Gaps = 2/374 (0%) Query: 264 TEASGSLPTQDEKGKSILAVYGTLQSSGWRVLGVVPSENLVKDAGRILGTTYXXXXXXXX 323 T AS QD GK + VY T+ +GW+V ++ L+ + ++ Sbjct: 229 TIASEKKGIQDVNGK--MVVYQTIGETGWKVGTQFDTDQLMWISDKMNRANLWISLIALI 286 Query: 324 XXXXXGLWMVRMVSRPLSRLRDLMFQGAEGDLRVRTSVASRDEIGQLSSSFNVMMERITE 383 ++ + ++ P+ +L + GDL VR S+DE+G L+ FN+M+E + E Sbjct: 287 ITIILSYFLAKTITGPIQQLIVKTKAVSAGDLTVRAESKSKDEVGILTRDFNLMVENMKE 346 Query: 384 LVVHTNETAREVLETADALGNASRKTXXXXXXXXXXXXXXXXXXXSLALEADRGNEMTAQ 443 +V ++ +V +T++ L + +T A E + NE + Sbjct: 347 MVEQVRLSSGKVSDTSEQLTAVAAETNERSGQIAKAIEEVAAGASEQASEVETINEKSES 406 Query: 444 ISERMDMVISAAREMGSMAHHVGQSSEEGVVKLQELLHQTYTTGTMTDKLVMKVNDLKDT 503 +S ++ + A + + +S +G+ L +LL ++ T K + DL++ Sbjct: 407 LSTKIRQIAEEAGGIKERSKSSEDASYKGLHALGQLLMKSNEANMETKKEETMLLDLENQ 466 Query: 504 ASSVMKVLEAMQSITQQTNILSLNASIXXXXXXXXXXXFTVVADEIRQLSEQSKRSIAMA 563 ++ +V+ A+ +I+ QTN+L+LN SI F VVADE+R+L+EQS S Sbjct: 467 TKNIEEVVTAISNISDQTNLLALNRSIEAARAGESGRGFAVVADEVRKLAEQSALSTKHI 526 Query: 564 AEITDKIMTDMHETVAALSEVSPLFDEQIVSVRNTSEIFVSVQGQMNHFISRLDSVGASI 623 +E I + E A+ E S + DEQ ++ T E+ ++ +M + +D + A I Sbjct: 527 SETVKLIQLETKEASHAMVEASRMNDEQNSAIHETGEVLNTITAEMQSLVQGIDHIYAEI 586 Query: 624 EGLNQSQYVLSDAI 637 + +++ Q +S+AI Sbjct: 587 QRMSEEQLAISEAI 600 >BH2275 [NT] COG0840 Methyl-accepting chemotaxis protein Length = 671 Score = 108 bits (270), Expect = 3e-23, Method: Compositional matrix adjust. Identities = 103/466 (22%), Positives = 190/466 (40%), Gaps = 17/466 (3%) Query: 165 WFKQLKEKP-HSLWITQGLKQGDTSGVFHFAKSMADESGGTGYIAVCDISTKELDSQLSK 223 W++ P +W + + D V AK++ DE G +A D++ + L Sbjct: 140 WYELAMASPTEVVWTSPYVSIEDGEIVMTVAKAILDERGQAQGVAGIDMTLNDFSDILGS 199 Query: 224 VNLGLDSYIQLLTSKDELIASSQQQTDSYLRLGGT-----LFKGLTEASGSLPTQDEKGK 278 + + D I L + I LG T L+ + +A+ G+ Sbjct: 200 IEMNYDGAIFLFDREGTAIVHPT-------LLGETVTEDELYGQVIQATSDSENGRTDGE 252 Query: 279 SILAVYGTLQSSGWRVLGVVPSENLVKDAGRILGTTYXXXXXXXXXXXXXGLWMVRMVSR 338 S + + T+ +GW V +N++KD + + + R +++ Sbjct: 253 STILFFQTVPLTGWIVGMEFERDNMLKDVNTVRNGIILVSVIALIVGMMIVILVARSITK 312 Query: 339 PLSRLRDLMFQGAEGDLRVRTSVASRDEIGQLSSSFNVMMERITELVVHTNETAREVLET 398 P+SRL + A GDL V A DE+GQL+ SF M+ ++ ++ + V E Sbjct: 313 PISRLGTHVGTVASGDLTVDVRAAGNDEVGQLTESFKEMLVQLRGIITTVKGSTAHVQEA 372 Query: 399 ADALGNASRKTXXXXXXXXXXXXXXXXXXXSLALEADRGNEMTAQISERMDMVISAAREM 458 AD L S +T D Q++ +M+ + + E+ Sbjct: 373 ADHLTAISEETIASSEEIALAVRDVAIGSTKQVEHLDEAKRQIDQLARQMEQMSLSMTEV 432 Query: 459 GSMAHHVGQSSEEGVVKLQELLHQTYTTGTMTDKLVMKVNDLKDTASSVMKVLEAMQSIT 518 GS+++ + + G K+Q L +T T+ D++ + L D + +V+ + ++ Sbjct: 433 GSLSNETRLAGQSGSEKVQHLKTKTLEANTVFDQMEDGLLKLSDHIMEINEVIHTINHLS 492 Query: 519 QQTNILSLNASIXXXXXXXXXXXFTVVADEIRQLSEQSKRSIAMAAEITDKIMTDMHETV 578 +QTN+L+LNASI F VVA+E+R+L+EQS + I+ + + Sbjct: 493 EQTNLLALNASIEAARAGEHGKGFAVVANEVRRLAEQSSHETVRVGDTITGIVNRSKQVI 552 Query: 579 AALSEVSPLFDEQIVSVRNTSEIFVSVQGQMNHFISRLDSVGASIE 624 + + + EQ SV T F+ M I +++ AS++ Sbjct: 553 VDMKQARSILSEQSDSVDETENTFL----HMASLIKQMNETLASVQ 594 >BS_tlpA [NT] COG0840 Methyl-accepting chemotaxis protein Length = 83 Score = 105 bits (261), Expect = 3e-22, Method: Compositional matrix adjust. Identities = 0/532 (0%), Positives = 0/532 (0%), Gaps = 24/532 (4%) Query: 104 STASSAERNSQTERINSELDRWITSVKGVQAVYLVPLKNEF---PVSATGTKDNAFMKGI 160 Sbjct: 81 ----- 135 Query: 161 REASWFKQLKEKPHSLWITQGLKQGDTSG--VFHFAKSMADESGGTGYIAVCDISTKELD 218 Sbjct: 136 ------ 189 Query: 219 SQLSKVNLGLDSYIQLLTSKDELIASSQQQTDSYLRLGGTLFKGLTEASGSLPTQDEKGK 278 Sbjct: 190 -- 247 Query: 279 SILAVYGTLQSSGWRVLGVVPSENLVKDAGRILGTTYXXXXXXXXXXXXXGLWMVRMVSR 338 Sbjct: 248 307 Query: 339 PLSRLRDLMFQGAEGDLRVRTSVASRDEIGQLSSSFNVMMERITELVVHTNETAREVLET 398 Sbjct: 308 367 Query: 399 ADALGNASRKTXXXXXXXXXXXXXXXXXXXSLALEADRGNEMTAQISERMDMVISAAREM 458 Sbjct: 368 --- 424 Query: 459 GSMAHHVGQSSE---EGVVKLQELLHQTYTTGTMTDKLVMKVNDLKDTASSVMKVLEAMQ 515 Sbjct: 425 484 Query: 516 SITQQTNILSLNASIXXXXXXXXXXXFTVVADEIRQLSEQSKRSIAMAAEITDKIMTDMH 575 Sbjct: 485 544 Query: 576 ETVAALSEVSPLFDEQIVSVRNTSEIFVSVQGQMNHFISRLDSVGASIEGLN 627 Sbjct: 545 596 >BS_mcpB [NT] COG0840 Methyl-accepting chemotaxis protein Length = 662 Score = 94.0 bits (232), Expect = 9e-19, Method: Compositional matrix adjust. Identities = 114/498 (22%), Positives = 216/498 (43%), Gaps = 56/498 (11%) Query: 161 REASWFKQLKEKPHSLWITQG-LKQGDTSGVFHFAKSMADESGGTGYIAVCDISTKELDS 219 R+ W++ + + +T + D S V A+ + D G+G +A+ DI+ +L Sbjct: 136 RQRPWYQDAMKAGGEIVVTDPYVAASDGSMVITIAQELKD---GSGVVAM-DITIDKLLE 191 Query: 220 QLSKVNLGLDSYIQLLTSKDELIASSQQQTDSYLRLGGTLFKGLTEASG----SLPTQDE 275 Q+ ++ +G + Y + T +A + L G + K SG +L +D+ Sbjct: 192 QMKQIKVGKEGYAFIATKNKTYVAHKNHKAGEKLS-GDWVAKMYANDSGELQYTLNNEDK 250 Query: 276 KGKSILAVYGTLQSSGWRVLGVVPSENLVKDAGR-ILGTTYXXXXXXXXXXXXXGLWMVR 334 K Y T + +GW++ G + + +KDA + +L T L++VR Sbjct: 251 K-----MTYTTNELTGWKIAGTMYMDE-IKDASKSVLTTGMIVLIASIVAGGILILFIVR 304 Query: 335 MVSRPLSRLRDLMFQGAEGDLRVRTSVASRDEIGQLSSSFNVMMERITELVVHTNETARE 394 +++PL RL + GDL + S+DE+G+L SFN M + + L+ + Sbjct: 305 SITKPLKRLVQSSKTISRGDLTETIEIHSKDELGELGESFNEMGQSLRSLISAIQNSVNN 364 Query: 395 VLETADALGNASRKTXXXXXXXXXXXXXXXXXXXSLALEA-DRGNEMTAQISERMDMVIS 453 V +++ L ++ +T ++A+E GNE E+ V S Sbjct: 365 VAASSEQLTASAGQTSKATEHI------------TMAIEQFSNGNE------EQSRKVES 406 Query: 454 AAREMGSMAHHVGQSSEEGVVKLQELLHQTYTTGTMTDKLVMK----------------- 496 ++ ++ M + Q S+ + + T GT +K V + Sbjct: 407 SSHQLNLMNEGLQQVSQTSSDITKASIQSTEIAGT-GEKFVQQTGGQMNSINQSVQQAEA 465 Query: 497 -VNDLKDTASSVMKVLEAMQSITQQTNILSLNASIXXXXXXXXXXXFTVVADEIRQLSEQ 555 V L+ + + +L + I QTN+L+LNA+I F+VVA+E+R+L+ Q Sbjct: 466 VVKGLEGKSKDITSILRVINGIADQTNLLALNAAIEAARAGESGRGFSVVAEEVRKLAVQ 525 Query: 556 SKRSIAMAAEITDKIMTDMHETVAALSEVSPLFDEQIVSVRNTSEIFVSVQGQMNHFISR 615 S S ++ +I+ ++ ++ EV+ +V NT E F S+ N + Sbjct: 526 SADSAKEIEKLIQEIVAEIDTSLHMFKEVNQEVQSGLVVTDNTKESFQSIFSMTNEIAGK 585 Query: 616 LDSVGASIEGL-NQSQYV 632 L ++ +++E L ++SQ+V Sbjct: 586 LQTMNSTVEQLSDRSQHV 603 >BS_mcpA [NT] COG0840 Methyl-accepting chemotaxis protein Length = 661 Score = 92.0 bits (227), Expect = 3e-18, Method: Compositional matrix adjust. Identities = 147/624 (23%), Positives = 268/624 (42%), Gaps = 64/624 (10%) Query: 27 RLFLVFFIATMGI-VLSLGYTSYSVARQTIENNALSSNQQTVEQTAEKLDVILLRFEDSL 85 R LV F++ + I V+ L +Y A +++ + S + V+Q E ++ + E+S Sbjct: 15 RKLLVSFLSILIIPVVILAIFAYQSASSSLDRQMMGSALENVQQLNEIINTSIGEKENS- 73 Query: 86 GQLFYNRDIQEAVRQGSISTASSAERNSQTERINSELDRWITSVKGVQAVYLVPLKNEFP 145 F +E + + S AS E+ SQ IN K V+++Y K F Sbjct: 74 ADYFSEWLTKE--KYNAKSNASIREKFSQYISIN----------KDVESIYTSDTKGHF- 120 Query: 146 VSATGTKDNAFMKGIR--EASWFKQLKEKPHSLWITQGLKQGDTSG-VFHFAKSMADESG 202 T D G E W+K+ + IT K T+ V A+ D Sbjct: 121 ---TRYPDLPMPSGYNPVERDWYKKAVANKGKVVITDPYKTASTNTMVVTIAQQTKD--- 174 Query: 203 GTGYIAVCDISTKELDSQLSKVNLGLDSYIQLLTSKDELIASSQQQTDSYLRLGGTLFKG 262 G+G IA+ +++ + L KVN+G Y ++T +++A +Q+ + L+ G L K Sbjct: 175 GSGVIAI-NMTIENLLKTTKKVNIGTQGYAFIMTKDKKVVAHPNEQSGTELK-GDWLDKM 232 Query: 263 LTEASGSLPTQDEKGKSILAVYGTLQSSGWRVLGVVPSENLVKDAGRILGTTYXXXXXXX 322 L+ G + K +A + T + +GW++ G + + + + A +L Sbjct: 233 LSADKGDFQYTMDGDKKKMA-FDTNKLTGWKIGGTMYLDEIHEAAQPVLHLALIVLAAAI 291 Query: 323 XXXXXXGLWMVRMVSRPLSRLRDLMFQGAEGDLRVRTSVASRDEIGQLSSSFNVMMERIT 382 ++R ++ PL +L + +EGDL + S+DE+G+L SFN M + Sbjct: 292 IIGIIVMTLIIRSITTPLKQLVGSSKRISEGDLTETIDIRSKDELGELGKSFNNMASSLR 351 Query: 383 ELVVHTNETAREVLETADALGNASRKTXXXXXXXXXXXXXXXXXXXSLALEA-DRGNEMT 441 L+ ++ V +++ L ++ +T +LA+E GNE Sbjct: 352 SLIHAIQDSVDNVAASSEELTASAAQTSKATEHI------------TLAIEQFSNGNE-- 397 Query: 442 AQISERMDMVISAAREMGSMAHHVGQSSE---EGVVKLQE-------LLHQTYTTGTMTD 491 + +E ++ +M ++ Q+SE + V+ E L+HQT + D Sbjct: 398 -KQNENIETAAEHIYQMNDGLTNMAQASEVITDSSVQSTEIASEGGKLVHQTVGQMNVID 456 Query: 492 KLVMK----VNDLKDTASSVMKVLEAMQSITQQTNILSLNASIXXXXXXXXXXXFTVVAD 547 K V + V L+ + + +L + I QTN+L+LNA+I F+VVA+ Sbjct: 457 KSVKEAEQVVRGLETKSKDITNILRVINGIADQTNLLALNAAIEAARAGEYGRGFSVVAE 516 Query: 548 EIRQLSEQSKRSI----AMAAEITDKIMTDMHETVAALSEVSP---LFDEQIVSVRNTSE 600 E+R+L+ QS S + EI +I T + + EV + D+ +S + SE Sbjct: 517 EVRKLAVQSADSAKEIEGLIIEIVKEINTSLGMFQSVNQEVQTGLDITDKTEMSFKRISE 576 Query: 601 IFVSVQGQMNHFISRLDSVGASIE 624 + + G++ + + + + AS E Sbjct: 577 MTNQIAGELQNMSATVQQLSASSE 600 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.314 0.128 0.340 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 37,915,464 Number of extensions: 1359057 Number of successful extensions: 3944 Number of sequences better than 1.0e-05: 777 Number of HSP's gapped: 3079 Number of HSP's successfully gapped: 777 Length of query: 694 Length of database: 38,378,431 Length adjustment: 113 Effective length of query: 581 Effective length of database: 25,345,689 Effective search space: 14725845309 Effective search space used: 14725845309 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (22.0 bits) S2: 119 (50.4 bits)