BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0305.0022.p (457 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SP1897 [G] COG1653 ABC-type sugar transport system, periplasmic ... 62 2e-09 PAE1324 [G] COG1653 ABC-type sugar transport system, periplasmic... 57 7e-08 VCA1033 [G] COG1653 ABC-type sugar transport system, periplasmic... 56 1e-07 PH1214 [G] COG1653 ABC-type sugar transport system, periplasmic ... 54 4e-07 alr2722 [G] COG1653 ABC-type sugar transport system, periplasmic... 54 6e-07 >SP1897 [G] COG1653 ABC-type sugar transport system, periplasmic component Length = 419 Score = 62.0 bits (149), Expect = 2e-09, Method: Compositional matrix adjust. Identities = 80/307 (26%), Positives = 133/307 (43%), Gaps = 14/307 (4%) Query: 57 KDIKGEITV--ITQRTDIVDTVFKDFAAKFNEKYPDVKVNFEALSSYEDQIKIRMSTNDY 114 K G +T+ Q+ ++ T+ ++ F ++ P +KV + + + +K R+ D Sbjct: 28 KSADGTVTIEYFNQKKEMTKTL-EEITRDFEKENPKIKVKVVNVPNAGEVLKTRVLAGDV 86 Query: 115 GDVL-LLPTSVAIKDLPD--FFEPLGKMSDLEKQYTGLEER-AVDGIAYGIPTTVNYSGV 170 DV+ + P S+ +++ FE L L++ G E+ AV+ Y +P T N G+ Sbjct: 87 PDVVNIYPQSIELQEWAKAGVFEDLSNKDYLKRVKNGYAEKYAVNEKVYNVPFTANAYGI 146 Query: 171 IYNKQVFKDAGITEIPRTFDEFNAALKSIKDKTDAIPLYTNYAAGWTLTQW-EADLATVA 229 YNK F++ G+ ++P T+DEF +K I K P A WTL + + AT Sbjct: 147 YYNKDKFEELGL-KVPETWDEFEQLVKDIVAKGQT-PFGIAGADAWTLNGYNQLAFATAT 204 Query: 230 GDRDYVNIGQVASDDNFVK-GQP-HYDLYKLMYDAAKNGLIEEDPTTTDWESSKADLANG 287 G N S N +K P D K+M NG +++ + A G Sbjct: 205 GGGKEANQYLRYSQPNAIKLSDPIMKDDIKVMDILRINGSKQKNWEGAGYTDVIGAFARG 264 Query: 288 KIGTMMLGSWAIGQIKGVAANPDDIGFMPFPTNAK-KILVPLSDDYTLGVSIHSKNKEAA 346 + GSWAI I N IG P K + L + D +S +K+ + A Sbjct: 265 DVLMTPNGSWAITAINEQKPN-FKIGTFMIPGKEKGQSLTVGAGDLAWSISATTKHPKEA 323 Query: 347 RAWVDWF 353 A+V++ Sbjct: 324 NAFVEYM 330 >PAE1324 [G] COG1653 ABC-type sugar transport system, periplasmic component Length = 437 Score = 57.0 bits (136), Expect = 7e-08, Method: Compositional matrix adjust. Identities = 56/203 (27%), Positives = 85/203 (41%), Gaps = 21/203 (10%) Query: 155 DGIAYGIP-TTVNYSGVIYNKQVFKDAGITEIPRTFDEFNAALKSIKDKTDAIPLYTNYA 213 +G + P T V G Y K F+ G++E P+T+DEF LK +K+ P+ + Sbjct: 163 EGKIWATPFTIVLKPGFWYRKSFFQQHGLSE-PQTWDEFLQLLKRLKE-IGVTPIASGDG 220 Query: 214 AGWTLTQW-EADLATVAGDRDYVNIGQ--VASDDNFVKGQPHYDLYKLMYDAAKNGLIEE 270 GW L+ EA + AG + + + V DD + D L+ G EE Sbjct: 221 VGWPLSDVTEAFIIAYAGPDVFRGLTEKKVKFDDPRINAV-FRDRISLLLREGYFGEPEE 279 Query: 271 DPTTTDWESSKADLANGKIGTMMLGSWAIGQIKGVAANPDDIGFMPFPTNAKKILVPLSD 330 W + NG G +G+W +G + NPDD+GF P P + Sbjct: 280 ------WTQAVEKWWNGTYGIYFMGTWILGMVD----NPDDVGFFPMPETKG---ITGGT 326 Query: 331 DYTLGVSIHSKNKEAARAWVDWF 353 DY V ++S + A +V W Sbjct: 327 DYIF-VPVYSPVRNEAVEFVQWL 348 >VCA1033 [G] COG1653 ABC-type sugar transport system, periplasmic component Length = 458 Score = 56.2 bits (134), Expect = 1e-07, Method: Compositional matrix adjust. Identities = 53/206 (25%), Positives = 87/206 (42%), Gaps = 27/206 (13%) Query: 159 YGIPTTVNYSGVIYNKQVFKDAGITEIPRTFDEFNAALKSIKDKTDAIPLYTNYAAGWTL 218 + +P G YNK++F + G++ +P+T ++F L +K IPL + + W Sbjct: 125 FCVPMASVIHGFFYNKKIFNELGLS-VPQTREQFFTVLDKVKADGRYIPLSMSGSESW-- 181 Query: 219 TQWEADLATVAGDRDYVNIGQ--VASDDN---FVKGQPHYD---LYKLMYDAAK-NGLIE 269 VA + Y NIG +D + GQ H D K+ + A+ + Sbjct: 182 ---------VASELGYQNIGPNYWKGEDGRLALINGQEHLDDSQYVKVFEELARWRAYLG 232 Query: 270 EDPTTTDWESSKADLANGKIGTMMLGSWAIGQIKGVAANPDDIGFMPFPTNAKKILVPLS 329 ED D+ +S +GK + GSW I + D G M P + + Sbjct: 233 EDGELRDYGTSNELFTSGKAALYVAGSWEIAPF----TDKVDFGVMRPPVAKQGDGCFFT 288 Query: 330 D--DYTLGVSIHSKNKEAARAWVDWF 353 D D +G++ SKN +AA A++ W Sbjct: 289 DHTDIGMGMNPASKNPQAAMAFLQWL 314 >PH1214 [G] COG1653 ABC-type sugar transport system, periplasmic component Length = 441 Score = 54.3 bits (129), Expect = 4e-07, Method: Compositional matrix adjust. Identities = 75/328 (22%), Positives = 131/328 (39%), Gaps = 55/328 (16%) Query: 77 FKDFAAKFNEKYPDVKVNFEALSSYEDQIKIRMSTNDYGDVLLLPTSVAIKDLPDFFE-- 134 F A KF +KYP++K+ +S + ++M +L + + PD F+ Sbjct: 59 FDALAKKFEKKYPNIKIKANPVSG-GGGVNLKM---------VLQSLMLAGKPPDTFQVH 108 Query: 135 ---------------PLGKM--SDLEKQYTGLEERAV--DGIAYGIPTTVNYSGVI-YNK 174 P+ + D++K Y + ++ V + Y +P V+ + VI YNK Sbjct: 109 AGYEMARYIKANQLTPIDDIWTEDMKKNYPKVIQQMVIFNNHYYAVPINVHRANVIWYNK 168 Query: 175 QVFKDAGITEIP-RTFDEFNAALKSIKDKTDAIPLYTNYAAGWTLTQ-WEADLATVAGDR 232 Q+FK GI +T D+ A + +K+ P W TQ +E L V G Sbjct: 169 QIFKKYGIDPSSIKTIDDLFAVAEKLKE-NGVTPFALGDRNKWPATQVFEVMLVAVGGVD 227 Query: 233 DYVNI--GQVASDDNFVKGQPHYDLYKLMYDAAKNGLIEEDPTTTDWESSKADLANGKIG 290 Y G+++++D +K Y + + Y D W+ + A + G+ Sbjct: 228 YYEKFINGEISANDPTLKKVLEYFVKYVEYS-------NPDHAAKTWDEACALVYKGEAA 280 Query: 291 TMMLGSWAIGQIKGVAANPD-DIGFMPFPTNAKKILVPLSDDYTLGVSIHSKNKEAARAW 349 ++G WA G K P+ D G +P P +++ D + L N AA+ W Sbjct: 281 MTLMGDWANGYFKAKGWKPNVDYGAIPIPAGVYDLVI---DTFVLPAKAAHPN--AAKKW 335 Query: 350 VDWFINESGYPTNDGGGMSPVKGAELPE 377 + + T +P+KG+ P Sbjct: 336 LSFIGTVEAQNT-----FNPIKGSIPPR 358 >alr2722 [G] COG1653 ABC-type sugar transport system, periplasmic component Length = 359 Score = 53.5 bits (127), Expect = 6e-07, Method: Compositional matrix adjust. Identities = 0/349 (0%), Positives = 0/349 (0%), Gaps = 55/349 (15%) Query: 76 VFKDFAAKFNEKYPDVKVNFEALSSYEDQIKIRMST---NDYGDVLLLPTSV-------- 124 + A + ++ F+A + + + L + Sbjct: 63 ALRLRAGGITKPVLLLEGFFDARDLPTISAQHFHTAVHNEEQLAALEEASLDEPVTVWMK 122 Query: 125 AIKDLPDFFEPLGKMSDLEKQYTGLEERAVDGIAYGIPTTVNYSGVIYNKQVFKDAGITE 184 + + + T + V + + A Sbjct: 123 LDTGMHRLGVRPEQAEAFYHRLTQ--CKNVRQPVNIVSHFARADEPKCGATEKQLAIFNT 180 Query: 185 IPRTFDEFNAALK--SIKDKTDAIP-----LYTNYAAGWTLTQWEADLATVAG------D 231 + I + Y + Sbjct: 181 FCEGKPGQRSIAASGGILLWPQSHFDWVRPGIILYGVSPLEDRSTGADFGCQPVMSLTSS 240 Query: 232 RDYVNIGQVASDDNFVKGQPHYDLYKLMYDAAKNGLIEEDPTTTDWESSKADLANGKIGT 291 V + G + D Sbjct: 241 LIAVREHKAGE-PVGYGGTWVSERDTR--LGVVAMG-YGD-----------GYPRAAPSG 285 Query: 292 MMLGSWAIGQIKGVAANPDDIGFMPFPTNAKKILVPLSDDYTLGVSIHSKNKEAARAWVD 351 + + + + + E Sbjct: 286 TPVLVNG-----REVPIVGRVAMDMICVDLGPQAQ-DKAGDPVILWGEGLPVERIAEMTK 339 Query: 352 WFINESGYPT--NDGGGMSPV--KGAELP---EMLKQFEGTDVTFDTLA 393 E + Sbjct: 340 VSAYEL-ITRLTSRVAMKYVD 387 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.315 0.134 0.394 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 33,579,167 Number of extensions: 1461604 Number of successful extensions: 3035 Number of sequences better than 1.0e-05: 10 Number of HSP's gapped: 3039 Number of HSP's successfully gapped: 10 Length of query: 457 Length of database: 38,378,431 Length adjustment: 110 Effective length of query: 347 Effective length of database: 25,691,691 Effective search space: 8915016777 Effective search space used: 8915016777 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (22.0 bits) S2: 117 (49.7 bits)