BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0305.0020.p (361 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value lin0851 [K] COG1609 Transcriptional regulators 90 6e-18 TM1856 [K] COG1609 Transcriptional regulators 84 3e-16 mll4964 [K] COG1609 Transcriptional regulators 75 1e-13 TM1200 [K] COG1609 Transcriptional regulators 71 2e-12 SMb20014 [K] COG1609 Transcriptional regulators 71 3e-12 >lin0851 [K] COG1609 Transcriptional regulators Length = 341 Score = 90.1 bits (222), Expect = 6e-18, Method: Compositional matrix adjust. Identities = 84/340 (24%), Positives = 153/340 (45%), Gaps = 22/340 (6%) Query: 5 KKVTLQHLATELGLTVHTVSKALRGLPGMSEYTRNEVLLLAQKLGY-HTKEQERSLLFEN 63 KKVT+Q +A L +T ++VS+AL G+SE T+ V +A +LGY + KE R ++ E Sbjct: 2 KKVTMQDIADRLNITKNSVSQALGNKNGVSEQTKLAVFKMADELGYKYKKEIAREVVKE- 60 Query: 64 TPVYSGPSRRFIFLIAARQGLQSPLHHILLESVQHRLGEAGHKVELLFVPEGLQPAKAFT 123 +F L + Q ++++++ + H+ EL+ P + A+ Sbjct: 61 ---------KFALLATSFALSQRSFFGEIIDNMKQSI--IAHQAELIIFPVTDEEAEKNQ 109 Query: 124 GWENKHNLSYADGIFISPVIEESVEAHLIQLKMPRILLNFPPPGANVDSVIWNVHDAMQQ 183 E ++ GIF+ I + +I+L MP +L++ P D+VI D Sbjct: 110 LPEQLTTENWT-GIFLLSHINTAYSKKVIELGMPVVLIDHHDPHLKADAVISQNKDGAFT 168 Query: 184 SARHLIAMGHRRIMYIGNPWLSRGYQLRWQAFCMTLEAAGLQVEPDSHIFEMNLPQEEWT 243 + LI H++I ++G+ S Y+ R++ + L+ + P + + + +EE T Sbjct: 169 AVEFLIENKHQKIGFLGDTTFSPSYEERFEGYKKALQYHQI---PFNEKYAITRIKEEQT 225 Query: 244 ALWKRQTEAYS-PTAFICASEQA--LTRVYLACQTTGKHIPGDYSLMGLEPEPSLRNIIP 300 L++ E PTA+ C + + YL Q+ G +IP D S++ + P Sbjct: 226 TLYRTLDELDELPTAWFCVNSGLGFILNTYL--QSKGYNIPKDLSIICFDNTEFTVLSNP 283 Query: 301 DIARPTLPVKETGGRAADRMLWRIANPSLPYEHILLQGGL 340 + + G +A + M R+ P + H+ L L Sbjct: 284 PLTTMCTNLSFMGEKAVELMYNRVRKPDEGFVHLALATNL 323 >TM1856 [K] COG1609 Transcriptional regulators Length = 328 Score = 84.3 bits (207), Expect = 3e-16, Method: Compositional matrix adjust. Identities = 73/317 (23%), Positives = 137/317 (43%), Gaps = 22/317 (6%) Query: 8 TLQHLATELGLTVHTVSKALRGLPGMSEYTRNEVLLLAQKLGYHTKEQERSLLFENT--P 65 T++ +A G+++ TVS+ + G +SE TR +V ++LGY + + L + Sbjct: 3 TIEDVAKLAGVSIATVSRVINGSGYVSEKTRYKVWKAIEELGYKPEISAKLLASKGKLFN 62 Query: 66 VYSGPSRRFIFLIAARQGLQSPLHHILLESVQHRLGEAGHKVELLFVPEGLQPAKAFTGW 125 +Y S+R I G+ + +++ +++ G G VEL + Sbjct: 63 IYVFASKR-ILSPLQNNGILGEFYGVIIRAIEENCGRLGMNVELKML------------- 108 Query: 126 ENKHNLSYADG-IFISPVIEESVEAHLIQLKMPRILLNFPPPGANVDSVIWNVHDAMQQS 184 EN + ADG IFI + E + K P +L++ PG VD VI + +D + Sbjct: 109 ENFSESNGADGYIFIGGDLSEKTLKEIKSRKKPFVLVDHYIPGERVDCVISDGYDGAVYA 168 Query: 185 ARHLIAMGHRRIMYIGNPWLSRGYQLRWQAFCMTLEAAGLQVEPDSHIFEMNLPQEEWTA 244 +LI+ G +RI+++ P G+++R+ + +E AGL +E + +E Sbjct: 169 TNYLISKGFKRIVHVHGPLHPYGFKMRYDGYKDAMEKAGLM----PRFYECDDTEEGTRK 224 Query: 245 LWKRQTEAY-SPTAFICASEQALTRVYLACQTTGKHIPGDYSLMGLEPEPSLRNIIPDIA 303 + +Y +P A +++ V + +P D S++G + N P + Sbjct: 225 VIDIMLNSYGTPEAIFTSNDSIAFWVIKRLKEHNIKVPDDVSVIGFDDIVDAENFDPPLT 284 Query: 304 RPTLPVKETGGRAADRM 320 + E G A R+ Sbjct: 285 TLRVFKYEMGCLACKRL 301 >mll4964 [K] COG1609 Transcriptional regulators Length = 341 Score = 75.5 bits (184), Expect = 1e-13, Method: Compositional matrix adjust. Identities = 80/308 (25%), Positives = 128/308 (41%), Gaps = 19/308 (6%) Query: 9 LQHLATELGLTVHTVSKALRGLPG--MSEYTRNEVLLLAQKLGYHTKEQERSLLFENTPV 66 + +A G + TVS L PG +S+ TR V+ A+ LGY + PV Sbjct: 1 MTDIARAAGCSQATVSFVLNNSPGIKLSQQTRERVIEAARALGYSAP----AFSALRKPV 56 Query: 67 YSGPSRRFIFLIAARQGLQSPLHHILLESVQHRLGEAGHKVELLFVPEGLQPA----KAF 122 + + A Q SP + +E + AG+ +L V + + A +A Sbjct: 57 AAFDGLDGVIGFAVDQLATSPEAVVAIEGARQASWNAGN---VLLVAQTMGDAVMEPRAI 113 Query: 123 TGWENKHNLSYADGIFISPVIEE-SVEAHLIQLKMPRILLNFPPPGANVDSVIWNVHDAM 181 + + I+++ E + +L L +P ILLN +V+ + Sbjct: 114 QALTRRGISAL---IYMTIFTREITAPDYLYSLDIPVILLNCYTADYAFPAVVPSEIAGG 170 Query: 182 QQSARHLIAMGHRRIMYI-GNPWLSRGYQLRWQAFCMTLEAAGLQVEPDSHIFEMNLPQE 240 Q S RHLI+ GHRRI I G PW+ + Q R + + L A + +PD + Sbjct: 171 QSSTRHLISHGHRRIATITGEPWM-QAAQDRLKGYRRALATADIPFDPDLVVEGDWSASA 229 Query: 241 EWTALWKRQTEAYSPTAFICASEQALTRVYLACQTTGKHIPGDYSLMGLEPEPSLRNIIP 300 + A K PTA C +++ Y A + G HIP D S++G + E R++ P Sbjct: 230 GYAATVKLLALKDRPTAIFCQNDRTAIGCYEALKEAGLHIPQDISVVGYDDEEIARHLFP 289 Query: 301 DIARPTLP 308 + LP Sbjct: 290 PLTTSILP 297 >TM1200 [K] COG1609 Transcriptional regulators Length = 333 Score = 71.2 bits (173), Expect = 2e-12, Method: Compositional matrix adjust. Identities = 74/325 (22%), Positives = 143/325 (44%), Gaps = 23/325 (7%) Query: 4 RKKVTLQHLATELGLTVHTVSKALRGLPGMSEYTRNEVLLLAQKLGYHTKEQERSLLFEN 63 +K VT++ +A + G++++TVS+AL P +SE TR ++L +AQ+LGY SL + Sbjct: 3 KKYVTIRDIAEKAGVSINTVSRALNNKPDISEETRRKILKIAQELGYVKNATASSLRSKQ 62 Query: 64 TPVYSGPSRRFIFLIAARQGLQSPLHHILLESVQHRLGEAGHKVELLFVPEGLQPAKAFT 123 T + + +I A +P + +L+ ++ + G+++ L + + + Sbjct: 63 TNI--------VGVIIADSA--NPFYAEVLKGIEAASRKYGYQIML------MNTERIYE 106 Query: 124 GWENKHNL---SYADGIFISPVIEESVEAH-LIQLKMPRILLNFPPPGANVDSVIWNVHD 179 E + DG+ I+PV + S + LI+ K+P +++ VD + + Sbjct: 107 NEEKAIEVLLQRRVDGLLITPVQDRSDDIKTLIERKVPFVIVGRHFEELEVDEIHSDEVK 166 Query: 180 AMQQSARHLIAMGHRRIMYIGNPWLSRGYQLRWQAFCMTLEAAGLQVEPDSHIFEMNLPQ 239 + RHLI G + I+ I +R + + L+ G+ + I E ++ Sbjct: 167 GGYLATRHLIERGRKNIIMISGYLFKSAAYMRLEGYRKALKEYGIPF-SEKMIIETDIDI 225 Query: 240 EE-WTALWKRQTEAYSPTAFICASEQALTRVYLACQTTGKHIPGDYSLMGLEPEPSLRNI 298 E + A+ + + A C ++ V A + G IP D +++G + I Sbjct: 226 ESGYRAMREAIEKGLEFEAVFCYNDLLAFGVIKALKECGYRIPEDVAVVGYDDIVYSSFI 285 Query: 299 IPDIARPTLPVKETGGRAADRMLWR 323 P + + E G A RML + Sbjct: 286 CPSLTTVRIKKFEMGFEAF-RMLLQ 309 >SMb20014 [K] COG1609 Transcriptional regulators Length = 333 Score = 70.9 bits (172), Expect = 3e-12, Method: Compositional matrix adjust. Identities = 88/352 (25%), Positives = 157/352 (44%), Gaps = 27/352 (7%) Query: 3 IRKKVTLQHLATELGLTVHTVSKALRGLPGMSEYTRNEVLLLAQKLGYHTKEQERSLLFE 62 +R + ++ +A + G++V TVS+AL ++ TR VL LA++L Y R+L Sbjct: 1 MRDRPKIKDIAEKAGVSVATVSRALAASTLVTAETRQRVLDLARELDYRPNVSARNLR-- 58 Query: 63 NTPVYSGPSRRFIFLIAARQGLQSPLHHILLESVQHRLGEAGHKVELLFVPEGLQPAKAF 122 +RR + ++ + + +P + +L+ V+ EAG+ V L+ E PA+ Sbjct: 59 --------TRRSMTVLMVVRDVGNPFYLDILKGVEATAREAGYAV-LMGNTEN-DPARE- 107 Query: 123 TGWENKHNLSYADG-IFISPVIEESVE-AHLIQLKMPRILLNFPPPGANVDSVIWNVHDA 180 T + + +ADG I ++ + + + HL + ++ P VD+V A Sbjct: 108 TEYFSMLRDGHADGMILMTGKLPSAGDWNHLPVVVALEMIEGSGLPHVQVDNVA-----A 162 Query: 181 MQQSARHLIAMGHRRIMYIGNPWLSRGYQLRWQAFCMTLEAAGLQVEPDSHI---FEMNL 237 + + +HLI++GHRRI +I P R + F + + AGL + + F + Sbjct: 163 ARGAVQHLISLGHRRIAHISGPVPEPMSVYRREGFRLAMRDAGLTIPAGYEVRGDFLIES 222 Query: 238 PQEEWTALWKRQTEAYSPTAFICASEQALTRVYLACQTTGKHIPGDYSLMGLEPEPSLRN 297 +E +L+ PTA A+++ + G+ +PGD S++G + + Sbjct: 223 GEECCRSLFSLSD---PPTALFVANDEMAYGAVHELRRIGRDVPGDVSVVGFDDLYLSKA 279 Query: 298 IIPDIARPTLPVKETGGRAADRMLWRIANPSLPYEHILLQGGLH-NGSTVQP 348 P + P E G A +L +A S E I+L L GST P Sbjct: 280 FYPPLTTVGQPRSEIGRTAMAVLLGILAGGSEVAEPIVLPTVLKVRGSTAPP 331 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.320 0.135 0.415 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 28,491,717 Number of extensions: 1185947 Number of successful extensions: 2520 Number of sequences better than 1.0e-05: 43 Number of HSP's gapped: 2516 Number of HSP's successfully gapped: 43 Length of query: 361 Length of database: 38,378,431 Length adjustment: 108 Effective length of query: 253 Effective length of database: 25,922,359 Effective search space: 6558356827 Effective search space used: 6558356827 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 116 (49.3 bits)