BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0305.0019.p (276 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value BH3679 [T] COG4753 Response regulator containing CheY-like recei... 134 2e-31 SP0661 [T] COG4753 Response regulator containing CheY-like recei... 126 3e-29 SPy1556 [T] COG4753 Response regulator containing CheY-like rece... 124 2e-28 SPy1062 [T] COG4753 Response regulator containing CheY-like rece... 120 3e-27 BH1910 [T] COG4753 Response regulator containing CheY-like recei... 92 1e-18 >BH3679 [T] COG4753 Response regulator containing CheY-like receiver domain and AraC-type DNA-binding domain Length = 257 Score = 134 bits (336), Expect = 2e-31, Method: Compositional matrix adjust. Identities = 86/252 (34%), Positives = 139/252 (55%), Gaps = 10/252 (3%) Query: 27 VMIADDEEVIRRGLEKITSRMDLEVQVIGSYGNGLEAWNHLRELSQDDIDLLITDIKMPR 86 V+I DDE RRGL++ ++ + + + EA + L+++ IDL+ITDI+MP Sbjct: 6 VLIVDDEPRSRRGLKRTLEAWSPKLLNVQTAASAKEA---ITRLNEETIDLIITDIRMPE 62 Query: 87 MDGFKLIEEVRGHLKQLPIAVLSGFSEFEYARRAMRHGVLDYLLKPIEKAQLYDLLKSVE 146 M G L++ ++ + V+S +SEFEYA A+ GV YLLKP++K +L + ++S Sbjct: 63 MSGLHLVKALQEQNPKPVCIVISAYSEFEYAHTALELGVRHYLLKPVKKEKLIEAVESAL 122 Query: 147 ENKKLRPVAAEPEEAPHQAGEGGEHYVVEQTKSI------LEKEYGQNFELERLAETVGM 200 + KK + + E+ + + E + + SI ++ Q L+ +A++V + Sbjct: 123 QMKKEQDRFGQVEKVWDEKIDAIEKERLSSSDSIHEALHYIDAHLDQKIGLKDVAKSVHL 182 Query: 201 NASYISRLFKYKTGQTITDYLIGIRIAKAKELLIGQPDLKNYEIAERVGYSDPVYFNKLF 260 N SY+S LFK KT T ++YL R+ AK LL+ D +IAE+ GY YF K+F Sbjct: 183 NPSYLSALFKEKTNMTFSEYLTRKRLQHAKHLLVST-DRPISDIAEKCGYQTAKYFIKIF 241 Query: 261 KKMCGMTPKDYK 272 K+ GMTP Y+ Sbjct: 242 KEHEGMTPTQYR 253 >SP0661 [T] COG4753 Response regulator containing CheY-like receiver domain and AraC-type DNA-binding domain Length = 245 Score = 126 bits (317), Expect = 3e-29, Method: Compositional matrix adjust. Identities = 82/247 (33%), Positives = 142/247 (57%), Gaps = 12/247 (4%) Query: 27 VMIADDEEVIRRGLEKITSRMDLEVQVIGSYGNGLEAWNHLRELSQDDI-DLLITDIKMP 85 ++I +DE ++R+GL K+ + ++++IG NG +AW EL Q + D+++TDI MP Sbjct: 5 ILIVEDEYLVRQGLTKLVNVAAYDMEIIGQAENGRQAW----ELIQKQVPDIILTDINMP 60 Query: 86 RMDGFKLIEEVRGHLKQLPIAVLSGFSEFEYARRAMRHGVLDYLLKPIEKAQLYDLLKSV 145 ++G +L VR Q+ + L+G+ +F+YA A++ GV DYLLKP + + ++L + Sbjct: 61 HLNGIQLASLVRETYPQVHLVFLTGYDDFDYALSAVKLGVDDYLLKPFSRQDIEEMLGKI 120 Query: 146 EENKKLRPVAAEPEEAPHQAGEGGEHYVVEQTKSILEKEYGQNFELERLAETVGMNASYI 205 ++ + ++ EG + ++ +S L F L+ LA +G + +Y+ Sbjct: 121 KQKLDKEEKEEQLQDLLTNRFEGN---MAQKIQSHLADS---QFSLKSLASDLGFSPTYL 174 Query: 206 SRLFKYKTGQTITDYLIGIRIAKAKELLIGQPDLKNYEIAERVGYSDPVYFNKLFKKMCG 265 S L K + G DYL+ R+ +AK LL+ LK YEIAE+VG+ D YF + FK++ G Sbjct: 175 SSLIKKELGLPFQDYLVRERVKQAKLLLLTTD-LKIYEIAEKVGFEDMNYFTQRFKQIAG 233 Query: 266 MTPKDYK 272 +TP+ +K Sbjct: 234 VTPRQFK 240 >SPy1556 [T] COG4753 Response regulator containing CheY-like receiver domain and AraC-type DNA-binding domain Length = 246 Score = 124 bits (311), Expect = 2e-28, Method: Compositional matrix adjust. Identities = 79/246 (32%), Positives = 139/246 (56%), Gaps = 8/246 (3%) Query: 27 VMIADDEEVIRRGLEKITSRMDLEVQVIGSYGNGLEAWNHLRELSQDDIDLLITDIKMPR 86 ++I +DE ++R+G+ + ++ + NG AW+ + ++ D+++TDI MP+ Sbjct: 4 LLIVEDEYLVRQGIRSLVDFSQFKIDRVNEAENGQLAWDLFQ---KEPYDIVLTDINMPK 60 Query: 87 MDGFKLIEEVRGHLKQLPIAVLSGFSEFEYARRAMRHGVLDYLLKPIEKAQLYDLLKSVE 146 ++G +L E ++ Q + L+G+ +F YA A++ G DYLLKP KA + D+L + Sbjct: 61 LNGIQLAELIKQESPQTHLVFLTGYDDFNYALSALKLGADDYLLKPFSKADVEDMLGKLR 120 Query: 147 ENKKLRPVAAEPEEAPHQAGEGGEHYVVEQTKSILEKEYGQNFELERLAETVGMNASYIS 206 + +L ++ E + E + V +I E+ + L+ LA+ +G + +Y+S Sbjct: 121 KKLEL----SKKTETIQELVEQPQKEVSAIAMAIHERLADSDLTLKSLAQQLGFSPNYLS 176 Query: 207 RLFKYKTGQTITDYLIGIRIAKAKELLIGQPDLKNYEIAERVGYSDPVYFNKLFKKMCGM 266 L K + G DYL+ R+ KAK LL+ +LK YEIAE+VG+ D YF++ FK++ G+ Sbjct: 177 VLIKKELGMPFQDYLVQERLKKAKLLLLTS-NLKIYEIAEQVGFEDMNYFSQRFKQLVGV 235 Query: 267 TPKDYK 272 TP YK Sbjct: 236 TPSQYK 241 >SPy1062 [T] COG4753 Response regulator containing CheY-like receiver domain and AraC-type DNA-binding domain Length = 262 Score = 120 bits (300), Expect = 3e-27, Method: Compositional matrix adjust. Identities = 84/255 (32%), Positives = 133/255 (52%), Gaps = 17/255 (6%) Query: 26 RVMIADDEEVIRRGLEKITSRMDLEVQVIGSYGNGLEAWNHLRELSQDDIDLLITDIKMP 85 +++I +DE +IR+ L L++ VIG +G E ++E SQ DI ++TDI MP Sbjct: 3 KLVIIEDEHIIRKWLRYAIDYKALDILVIGEAKDGKEGAVLIKE-SQPDI--VLTDINMP 59 Query: 86 RMDGFKLIEEVRGHLKQLPIAVLSGFSEFEYARRAMRHGVLDYLLKPIEKAQLYDLLKSV 145 M F + E + + +LSG+++F AR A+ +GVL++L KPIEKA L++ L+++ Sbjct: 60 IMTAFDMFEVTKD--QTYAKIILSGYADFPNARSAIHYGVLEFLTKPIEKAALWECLQTI 117 Query: 146 --------EENKKLRPVAAEPEEAPHQAGEGGEHYVVEQTKSILEKEYGQNFELERLAET 197 N+K P P + E VV+ + + RLA Sbjct: 118 IAKIEKQKGSNQKTDACVYIP--LPQMTDQIPE--VVKDILEWVHAHFQDKISTSRLAHD 173 Query: 198 VGMNASYISRLFKYKTGQTITDYLIGIRIAKAKELLIGQPDLKNYEIAERVGYSDPVYFN 257 +G + SYI + K ++DY+ RI +A +L+ +PDL YEIA+ VG D YF+ Sbjct: 174 LGYSESYIYQNIKKHLQMPLSDYINQYRINQAIQLMQQEPDLMVYEIAQAVGIYDYRYFD 233 Query: 258 KLFKKMCGMTPKDYK 272 ++FKK G T K +K Sbjct: 234 RVFKKYLGQTVKAFK 248 >BH1910 [T] COG4753 Response regulator containing CheY-like receiver domain and AraC-type DNA-binding domain Length = 506 Score = 91.7 bits (226), Expect = 1e-18, Method: Compositional matrix adjust. Identities = 50/119 (42%), Positives = 73/119 (61%), Gaps = 3/119 (2%) Query: 27 VMIADDEEVIRRGLEKITSRMDLEVQVIGSYGNGLEAWNHLRELSQDDIDLLITDIKMPR 86 VMI DDE ++R GL+ + S + +VI + NG E + R+LS D L+I DI+MP Sbjct: 4 VMIVDDEAMVREGLKALISWEEYGFRVIDTARNGKEGFTKYRQLSPD---LMIVDIRMPE 60 Query: 87 MDGFKLIEEVRGHLKQLPIAVLSGFSEFEYARRAMRHGVLDYLLKPIEKAQLYDLLKSV 145 +DG LIE +R + +LSG S+F YA++A+ DYLLKP+++ +L D LK V Sbjct: 61 VDGLSLIESIRAEDSAIHFIILSGHSDFNYAKKALGCQCDDYLLKPLDEDELIDSLKKV 119 Score = 77.4 bits (189), Expect = 2e-14, Method: Compositional matrix adjust. Identities = 37/96 (38%), Positives = 58/96 (60%), Gaps = 2/96 (2%) Query: 179 SILEKEYGQNFELERLAETVGMNASYISRLFKYKTGQTITDYLIGIRIAKAKELLIGQPD 238 +++ K+Y +N LE LA N +Y+ +LFK TG+ YL +RI K LL Q Sbjct: 404 ALIHKDYAKNLRLETLASVFNYNRAYLGKLFKNYTGEYFNTYLDKVRIENGKRLL--QKG 461 Query: 239 LKNYEIAERVGYSDPVYFNKLFKKMCGMTPKDYKSR 274 K YE+AE++GY++ YF++ FKK G++P ++ R Sbjct: 462 YKVYEVAEKIGYANVDYFHRKFKKYVGVSPSTFRKR 497 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.319 0.138 0.393 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 21,299,178 Number of extensions: 905891 Number of successful extensions: 3780 Number of sequences better than 1.0e-05: 293 Number of HSP's gapped: 4677 Number of HSP's successfully gapped: 301 Length of query: 276 Length of database: 38,378,431 Length adjustment: 105 Effective length of query: 171 Effective length of database: 26,268,361 Effective search space: 4491889731 Effective search space used: 4491889731 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 115 (48.9 bits)