BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0305.0016.p (391 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value MA2382 [G] COG2152 Predicted glycosylase 120 5e-27 STM1225 [E] COG1176 ABC-type spermidine/putrescine transport sys... 116 5e-26 TM1225 [G] COG2152 Predicted glycosylase 116 5e-26 MA2834 [G] COG2152 Predicted glycosylase 106 8e-23 MA2831 [G] COG2152 Predicted glycosylase 101 2e-21 >MA2382 [G] COG2152 Predicted glycosylase Length = 335 Score = 120 bits (300), Expect = 5e-27, Method: Compositional matrix adjust. Identities = 104/354 (29%), Positives = 159/354 (44%), Gaps = 49/354 (13%) Query: 41 LLTAEHAPLIWRYDFNP----ETNPYFAERIGVNGIFNPGAIELDGKFYIVARVEGNDRK 96 ++ +H L R+ NP E PY A N +FNP A ++ K ++ RVE + Sbjct: 1 MIWKDHGELFVRHKKNPILTVEDWPYQA-----NSVFNPAAAMVESKTLLLVRVEDHRGF 55 Query: 97 SFFAVAESDSGVDGFRFWDHPVVLPETEDPDINVY---DMRLVKHADGWIYGLFCTERKD 153 S F VA S++G+DG+ P + P+T + +Y D R+ + + + T D Sbjct: 56 SHFTVARSNNGIDGWEIDTEPTLTPDTVNYPEEIYGIEDPRITYIDEMGRWAVAYTAFSD 115 Query: 154 PDAPHGDLSSAVAQCGITRTKDLKTWERLADLKTGSAQQRNVVLHPEFVDGKYAFYTRPQ 213 G L+S + TKD + +ER+ T + ++ L P +GK+A RP Sbjct: 116 S----GPLTS------LALTKDFRNFERIG--ATMPPENKDSALFPVRFNGKWAMLHRP- 162 Query: 214 XXXXXXXXXXXXXXXLSDTIENAVITSETIMDQRYYHTI----------KEVKNGQGPAP 263 +S N I+ M H + K G P P Sbjct: 163 ------------VSSMSGAKANIWISFSPDMRHWGDHQVLLYAREGGWWDARKIGLSPQP 210 Query: 264 IKTPRGWLHIAHGVRNTAAGLRYVLYAFLSDLEEPNKVTHTPGGHFIAP-DGEERVGDVS 322 I+T GWL + HGVR T + L Y L L DLE+P KV H G P + ER GDV+ Sbjct: 211 IRTYDGWLIMYHGVRPTTSKLSYRLGMALLDLEDPRKVLHRSEGWVFGPRELYERSGDVN 270 Query: 323 NVVFCNGVIARDNGDIYIYYASSDTRIHVATTTVDQMLDYVLNTPEDPLRSYAC 376 +VVF G + D+ I +YY S+DT + +A+ + +L+Y+ PE+ + C Sbjct: 271 DVVFPCGWVLHDD-QIRLYYGSADTSVSLASAKLHDVLEYIHECPEEQCPAEFC 323 >STM1225 [E] COG1176 ABC-type spermidine/putrescine transport system, permease component I Length = 287 Score = 116 bits (291), Expect = 5e-26, Method: Compositional matrix adjust. Identities = 0/330 (0%), Positives = 0/330 (0%), Gaps = 48/330 (14%) Query: 50 IWRYDFNPETNPYFAERIGVNGI------FNPGAIELDGKFYIVARVEGNDRKSFFAVAE 103 ++ + E A+ Sbjct: 24 FLPNLMII--------GTSFLTRDDASFVKMVFTLDNYARLLDPLYFEVLLHSLNMALIA 75 Query: 104 SDSGVDGFRFWDHPVVLPETEDPDIN--------VYDMRLVKHADGWIYGLFCTERKDPD 155 + S + + + ++ Sbjct: 76 TLSCLV----LG--YPFAWFLAKLPEKIRPLLLFLLIVPFWTNSLIR-IYGLKIFLSTK- 127 Query: 156 APHGDLSSAVAQCGITRTKDLKTWERLADLKTGSAQQRNVVLHPEFVDGKYAFYTRPQXX 215 L + + ++ + Sbjct: 128 -----------GYLNEFLLWLGVIDTPIRIMF--TPSAVIIGLVYILLPFMVMPLYSSIE 174 Query: 216 XXXXXXXXXXXXXLSDTIENAVITSETIMDQRYYHTIKEVKNGQGPAPIKTPRGWLHIAH 275 + I+ + PA L Sbjct: 175 KLDKPLLEAARDL-GASKMQT--FIRIIIPLTMPGIVAGCLLVMLPAMGLFYVSDLMGGA 231 Query: 276 GVRNTAAGLRYVLYAFLSDLEEPNKVTHTPGGHFIAPDGE-ERVGDVSNVVF-CNGVIAR 333 ++ + ++ V Sbjct: 232 KNLLIGNVIKVQFLNIRDWPFGAATSITLTIVMGLMLLIYWRASRLLNKKVSD 291 Query: 334 DNGDIYIYYASSDTRIHVATTTVDQMLDYV 363 Sbjct: 292 321 >TM1225 [G] COG2152 Predicted glycosylase Length = 326 Score = 116 bits (291), Expect = 5e-26, Method: Compositional matrix adjust. Identities = 99/330 (30%), Positives = 147/330 (44%), Gaps = 48/330 (14%) Query: 50 IWRYDFNPETNPYFAERIGVNGI------FNPGAIELDGKFYIVARVEGNDRKSFFAVAE 103 +WRY NP IG N + FN + +G+F V R++ + + F Sbjct: 24 VWRYSKNP--------IIGRNPVPKGARVFNSAVVPYNGEFVGVFRIDHKNTRPFLHFGR 75 Query: 104 SDSGVDGFRFWDHPVVLPETEDPDIN--------VYDMRLVKHADGWIYGLFCTERKDPD 155 S G++ W+ + E + D+N YD R+VK D + Y FCT+ P Sbjct: 76 SKDGIN----WE--IEPEEIQWVDVNGEPFQPSYAYDPRVVKIEDTY-YITFCTDDHGP- 127 Query: 156 APHGDLSSAVAQCGITRTKDLKTWERLADLKTGSAQQRNVVLHPEFVDGKYAFYTRPQXX 215 G+ TKD KT+ RL + RN VL P ++GKY RP Sbjct: 128 -----------TIGVGMTKDFKTFVRLPNAYV--PFNRNGVLFPRKINGKYVMLNRPSDN 174 Query: 216 XXXXXXXXXXXXXLSDTIENAVITSETIMDQRYYHTIKEVKNGQGPAPIKTPRGWLHIAH 275 D I ++ + Y+ + +K G GP PI+T GWL I H Sbjct: 175 GHTPFGDIFLSES-PDMIHWG--NHRFVLGRSSYNWWENLKIGAGPYPIETSEGWLLIYH 231 Query: 276 GVRNTAAGLRYVLYAFLSDLEEPNKVTHTPGGHFIAPDGE-ERVGDVSNVVF-CNGVIAR 333 GV T G Y A L DL++P+KV + + + P+ E E VG V NVVF C + Sbjct: 232 GVTLTCNGYVYSFGAALLDLDDPSKVLYRSRYYLLTPEEEYETVGFVPNVVFPCAALCDA 291 Query: 334 DNGDIYIYYASSDTRIHVATTTVDQMLDYV 363 D G + IYY ++DT + +A +D+++D+V Sbjct: 292 DTGRVAIYYGAADTHVALAFGYIDEIVDFV 321 >MA2834 [G] COG2152 Predicted glycosylase Length = 331 Score = 106 bits (264), Expect = 8e-23, Method: Compositional matrix adjust. Identities = 96/338 (28%), Positives = 142/338 (42%), Gaps = 65/338 (19%) Query: 52 RYDFNP----ETNPYFAERIGVNGIFNPGAIELDGKFYIVARVEGNDRKSFFAVAESDSG 107 R+ NP E PY A N +FNP A +DGK ++ RVE + S VA S++G Sbjct: 4 RHKKNPILTVEDWPYHA-----NSVFNPAAAIVDGKILLLVRVEDHRGISHLTVARSNNG 58 Query: 108 VDGFRFWDHPVVLPE----------TEDPDINVYDMRLVKHADGWIYGLFCTERKDPDAP 157 +DG+ P +P+ EDP I D W+ Sbjct: 59 IDGWEIDPEPTFVPDPVNYPEEIYGIEDPRITYID-----ELGKWVVA------------ 101 Query: 158 HGDLSSAVAQCGITRTKDLKTWERLADLKTGSAQQRNVVLHPEFVDGKYAFYTRPQXXXX 217 + S + + T+D + +ER+ + ++ L P +GK+A RP Sbjct: 102 YTAFSDSGPLPALAFTEDFRNFERIG--PALPPENKDAALFPVRFNGKWAMIHRP----- 154 Query: 218 XXXXXXXXXXXLSDTIENAVITSETIMDQRYYHTI----------KEVKNGQGPAPIKTP 267 + N I+ T M+ H + K G P PI+ P Sbjct: 155 --------VPAIHGMKANMWISFSTEMEAWGGHQVLLYAREGGWWDAYKIGLCPQPIRIP 206 Query: 268 RGWLHIAHGVRNTAAGLRYVLYAFLSDLEEPNKVTHTPGGHFIAP-DGEERVGDVSNVVF 326 GWL + HGVR T + Y L L D E+P KV G P + ER GDV+ VVF Sbjct: 207 DGWLIMYHGVRPTMPTISYRLGLALLDPEDPRKVLRRSEGWVFGPLELYERSGDVNYVVF 266 Query: 327 -CNGVIARDNGDIYIYYASSDTRIHVATTTVDQMLDYV 363 C V+ +D ++ IYY S+DT I +A+ + ++L+YV Sbjct: 267 PCGWVVVKD--ELRIYYGSADTSISMASAKMSEILEYV 302 >MA2831 [G] COG2152 Predicted glycosylase Length = 319 Score = 101 bits (251), Expect = 2e-21, Method: Compositional matrix adjust. Identities = 89/340 (26%), Positives = 144/340 (42%), Gaps = 57/340 (16%) Query: 52 RYDFNP----ETNPYFAERIGVNGIFNPGAIELDGKFYIVARVEGNDRKSFFAVAESDSG 107 R+ NP E PY A N +FNP A +DG+ ++ RVE + S +A S++G Sbjct: 4 RHKKNPILTIEDWPYHA-----NAVFNPAAAIVDGETLLMVRVEDHRGFSHLTIARSNNG 58 Query: 108 VDGFRFWDHPVVLPETEDPDINVYDMRLVKHADGWIYGL------FCTERKDPDAPHGDL 161 +DG+ P ++P DP H IYG+ + E + Sbjct: 59 IDGWEIDPEPTLVP---DP----------AHYPEEIYGIEDPRITYIEEMGKWAVAYTAF 105 Query: 162 SSAVAQCGITRTKDLKTWERLADLKTGSAQQRNVVLHPEFVDGKYAFYTRPQXXXXXXXX 221 S + + T+D + +ER+ + + ++ L P +GK+A RP Sbjct: 106 SDSGPLASLAFTEDFRNFERIGAIM--PPENKDAALFPVRFNGKWALLHRP--------- 154 Query: 222 XXXXXXXLSDTIENAVITSETIMDQRYYHTI----------KEVKNGQGPAPIKTPRGWL 271 ++ T N I+ M H + K G P P++ P GWL Sbjct: 155 ----VSGMTGTKTNIWISFSPDMKHWGGHQVLLYAREGGWWDARKIGLSPHPMRVPEGWL 210 Query: 272 HIAHGVRNTAAGLRYVLYAFLSDLEEPNKVTHTPGGHFIAP-DGEERVGDVSNVVF-CNG 329 + +G R T Y L L D E+P KV G P + ER GDV++V+ C Sbjct: 211 IMYYGARQTTGKTSYRLGLALLDPEDPRKVLRRSEGWVFGPRELYERSGDVNDVILPCGW 270 Query: 330 VIARDNGDIYIYYASSDTRIHVATTTVDQMLDYVLNTPED 369 V+ D +I IYY S++ + +A+ ++ +L+Y+ PE+ Sbjct: 271 VLVED--EIRIYYGSAEVSVSMASAKINDILEYIRGCPEE 308 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.320 0.138 0.424 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 30,330,783 Number of extensions: 1294015 Number of successful extensions: 2443 Number of sequences better than 1.0e-05: 6 Number of HSP's gapped: 2434 Number of HSP's successfully gapped: 12 Length of query: 391 Length of database: 38,378,431 Length adjustment: 108 Effective length of query: 283 Effective length of database: 25,922,359 Effective search space: 7336027597 Effective search space used: 7336027597 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 117 (49.7 bits)