BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0305.0011.p (765 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value BS_mcpC [NT] COG0840 Methyl-accepting chemotaxis protein 106 1e-22 BH1827 [NT] COG0840 Methyl-accepting chemotaxis protein 97 1e-19 BS_mcpB [NT] COG0840 Methyl-accepting chemotaxis protein 96 3e-19 BH0876_2 [NT] COG0840 Methyl-accepting chemotaxis protein 85 5e-16 BS_tlpA [NT] COG0840 Methyl-accepting chemotaxis protein 84 1e-15 >BS_mcpC [NT] COG0840 Methyl-accepting chemotaxis protein Length = 654 Score = 106 bits (265), Expect = 1e-22, Method: Compositional matrix adjust. Identities = 99/423 (23%), Positives = 192/423 (45%), Gaps = 10/423 (2%) Query: 346 KDANGEDVLAVYSPMVKADWKLAGIVPTGELVKAATPXXXXXXXXXXXXXXXXXXVGFVM 405 +D NG+ + VY + + WK+ T +L+ + + + + Sbjct: 238 QDVNGK--MVVYQTIGETGWKVGTQFDTDQLMWISDKMNRANLWISLIALIITIILSYFL 295 Query: 406 VRMIARPLARLKDLMVEGAKGDLSVRTEYVSKDEIGELSASFNTMMERITELVAQTTDTA 465 + I P+ +L + GDL+VR E SKDE+G L+ FN M+E + E+V Q ++ Sbjct: 296 AKTITGPIQQLIVKTKAVSAGDLTVRAESKSKDEVGILTRDFNLMVENMKEMVEQVRLSS 355 Query: 466 REVLETAGELGDASRXXXXXXXXXXXXXXXXXXXXXXLAQEAERGNELTDLIGTQMQSVI 525 +V +T+ +L + A E E NE ++ + T+++ + Sbjct: 356 GKVSDTSEQLTAVAAETNERSGQIAKAIEEVAAGASEQASEVETINEKSESLSTKIRQIA 415 Query: 526 AANAEMDEAARGVGEASGQGAKQLEELLTQTSR----TGEMTTALVDRVNNLKETVSSVI 581 + E ++ +AS +G L +LL +++ T + T L+D N K ++ Sbjct: 416 EEAGGIKERSKSSEDASYKGLHALGQLLMKSNEANMETKKEETMLLDLENQTK----NIE 471 Query: 582 KVLDVMKNITQQTNILSLNATIXXXXXXXXXXXFMVVADEVRQLADQSKQSIAVVAGITD 641 +V+ + NI+ QTN+L+LN +I F VVADEVR+LA+QS S ++ Sbjct: 472 EVVTAISNISDQTNLLALNRSIEAARAGESGRGFAVVADEVRKLAEQSALSTKHISETVK 531 Query: 642 KIITEMNETVAVLSEVAPLFKQQMNSVKSTSEIFVSVQGQMDDFISSLESVTGAIDSLNH 701 I E E + E + + +Q +++ T E+ ++ +M + ++ + I ++ Sbjct: 532 LIQLETKEASHAMVEASRMNDEQNSAIHETGEVLNTITAEMQSLVQGIDHIYAEIQRMSE 591 Query: 702 SQGVLSDAMSNXXXXXXXXXXTSEEVASLSNEQQNVSDQLVALSGKLEGASTQLKDKLSL 761 Q +S+A+ + +EEV + ++EQ D++ + L+ AS + + + Sbjct: 592 EQLAISEAIQSISAISQESAAAAEEVNASTDEQLVTLDKVKHSTETLKHASQDVINTIRK 651 Query: 762 FTI 764 FT+ Sbjct: 652 FTL 654 >BH1827 [NT] COG0840 Methyl-accepting chemotaxis protein Length = 678 Score = 97.1 bits (240), Expect = 1e-19, Method: Compositional matrix adjust. Identities = 104/495 (21%), Positives = 202/495 (40%), Gaps = 23/495 (4%) Query: 282 IMLELKNSLLEEAFESVTLGDGSRIQLVSPDGTVVASSNPEEDGKASELGFIKESKINNK 341 I+L+ + LL E +G G + L+ G V+A +P+ D + L ES I Sbjct: 189 ILLDDLSQLLNE----TEVGMGGSLALIHESGMVIA--HPDADLRGENLS--DESFI--- 237 Query: 342 SQDAKDANG-----------EDVLAVYSPMVKADWKLAGIVPTGELVKAATPXXXXXXXX 390 QD + A G E ++ M + DW + E+ Sbjct: 238 -QDLQQAQGKQGQIEYEFENEARTLFFNKMDQMDWNIVAFYRDKEVTAPVRQLRNYFILV 296 Query: 391 XXXXXXXXXXVGFVMVRMIARPLARLKDLMVEGAKGDLSVRTEYVSKDEIGELSASFNTM 450 + R +A P+ L D M + KGDL+ + S+DEIG+LS FN M Sbjct: 297 LLVSMTIATATSYYFGRKMANPILALNDRMQQVIKGDLTASADVKSQDEIGQLSDGFNQM 356 Query: 451 MERITELVAQTTDTAREVLETAGELGDASRXXXXXXXXXXXXXXXXXXXXXXLAQEAERG 510 ++ + E++ T + A EL + A+E E Sbjct: 357 VQTLREMIHALRQTGATSNKEAVELRSMLQEAIASSEEVATSSTAVANGAVDQAEEIEHA 416 Query: 511 NELTDLIGTQMQSVIAANAEMDEAARGVGEASGQGAKQLEELLTQTSRTGEMTTALVDRV 570 + + Q+ + ++++E A S G +Q+E L +T + + V Sbjct: 417 KSDIEKLSAQIDWMDQTASQINELAASTANVSASGLEQVETLREKTEEHERIIQKMEGIV 476 Query: 571 NNLKETVSSVIKVLDVMKNITQQTNILSLNATIXXXXXXXXXXXFMVVADEVRQLADQSK 630 N+ ++ +++++++ ++QTN+L+LNA+I F VVA+E+R+LA+QS Sbjct: 477 NHFVHQTEEILSIVELIQSFSEQTNLLALNASIEAARAGEYGKGFSVVAEEIRKLAEQST 536 Query: 631 QSIAVVAGITDKIITEMNETVAVLSEVAPLFKQQMNSVKSTSEIFVSVQGQMDDFISSLE 690 ++ + KI +++ A +Q+ V + F + ++ ++S++ Sbjct: 537 KATNNIQSAVQKIKESGAHATEEMTKTAAQSAEQVKRVDQSIASFTHISSNIEQVVNSIK 596 Query: 691 SVTGAIDSLNHSQGVLSDAMSNXXXXXXXXXXTSEEVASLSNEQQNVSDQLVALSGKLEG 750 +V + ++N S+ A++N +E++++ E NV + + S +L Sbjct: 597 TVVKQLQTMNESRNQAVLAINNIAGTSQDTAAAAEQISASIEELVNVLNTIGTSSERLNT 656 Query: 751 ASTQLKDKLSLFTIK 765 S L + F ++ Sbjct: 657 LSDDLNKQAKAFKVQ 671 >BS_mcpB [NT] COG0840 Methyl-accepting chemotaxis protein Length = 662 Score = 95.5 bits (236), Expect = 3e-19, Method: Compositional matrix adjust. Identities = 111/512 (21%), Positives = 216/512 (42%), Gaps = 5/512 (0%) Query: 254 PTAVAGDNGKNFAMVRSLKNLGANSGYVIMLELKNSLLEEAFESVTLGDGSRIQLVSPDG 313 P A D + + LK+ SG V M + LLE+ + + +G + + + Sbjct: 156 PYVAASDGSMVITIAQELKD---GSGVVAMDITIDKLLEQ-MKQIKVGKEGYAFIATKNK 211 Query: 314 TVVASSNPEEDGKASELGFIKESKINNKSQDAKDANGEDVLAVYSPMVKADWKLAGIVPT 373 T VA N + K S ++ + N+ + N ED Y+ WK+AG + Sbjct: 212 TYVAHKNHKAGEKLSG-DWVAKMYANDSGELQYTLNNEDKKMTYTTNELTGWKIAGTMYM 270 Query: 374 GELVKAATPXXXXXXXXXXXXXXXXXXVGFVMVRMIARPLARLKDLMVEGAKGDLSVRTE 433 E+ A+ + +VR I +PL RL ++GDL+ E Sbjct: 271 DEIKDASKSVLTTGMIVLIASIVAGGILILFIVRSITKPLKRLVQSSKTISRGDLTETIE 330 Query: 434 YVSKDEIGELSASFNTMMERITELVAQTTDTAREVLETAGELGDASRXXXXXXXXXXXXX 493 SKDE+GEL SFN M + + L++ ++ V ++ +L ++ Sbjct: 331 IHSKDELGELGESFNEMGQSLRSLISAIQNSVNNVAASSEQLTASAGQTSKATEHITMAI 390 Query: 494 XXXXXXXXXLAQEAERGNELTDLIGTQMQSVIAANAEMDEAARGVGEASGQGAKQLEELL 553 +++ E + +L+ +Q V ++++ +A+ E +G G K +++ Sbjct: 391 EQFSNGNEEQSRKVESSSHQLNLMNEGLQQVSQTSSDITKASIQSTEIAGTGEKFVQQTG 450 Query: 554 TQTSRTGEMTTALVDRVNNLKETVSSVIKVLDVMKNITQQTNILSLNATIXXXXXXXXXX 613 Q + + V L+ + +L V+ I QTN+L+LNA I Sbjct: 451 GQMNSINQSVQQAEAVVKGLEGKSKDITSILRVINGIADQTNLLALNAAIEAARAGESGR 510 Query: 614 XFMVVADEVRQLADQSKQSIAVVAGITDKIITEMNETVAVLSEVAPLFKQQMNSVKSTSE 673 F VVA+EVR+LA QS S + + +I+ E++ ++ + EV + + +T E Sbjct: 511 GFSVVAEEVRKLAVQSADSAKEIEKLIQEIVAEIDTSLHMFKEVNQEVQSGLVVTDNTKE 570 Query: 674 IFVSVQGQMDDFISSLESVTGAIDSLNHSQGVLSDAMSNXXXXXXXXXXTSEEVASLSNE 733 F S+ ++ L+++ ++ L+ +S A+S + +++A+ + E Sbjct: 571 SFQSIFSMTNEIAGKLQTMNSTVEQLSDRSQHVSAAVSGIADVSKESSASIQDIAASAEE 630 Query: 734 QQNVSDQLVALSGKLEGASTQLKDKLSLFTIK 765 Q +++ + + L + +L+D F I+ Sbjct: 631 QLASMEEISSSATTLAQMAEELRDLTKQFKIE 662 >BH0876_2 [NT] COG0840 Methyl-accepting chemotaxis protein Length = 359 Score = 84.7 bits (208), Expect = 5e-16, Method: Compositional matrix adjust. Identities = 79/314 (25%), Positives = 146/314 (46%), Gaps = 44/314 (14%) Query: 407 RMIARPLARLKDLMVEGAKGDLSV-RTEYVSKDEIGELSASFNTMMERITELVAQTTDTA 465 +MI +PLARL + A GDL+ R + ++DEIG+L+ SF M E + L+ Sbjct: 1 KMITKPLARLTKVARHVADGDLTTERVKIKNRDEIGQLNESFEAMKENLRGLI------- 53 Query: 466 REVLETAGELGDASRXXXXXXXXXXXXXXXXXXXXXXLAQEAERGNELTDLIGTQMQSVI 525 + +L+ A ++ +S ++ RG D+ + Q+ + Sbjct: 54 QRILQNADQVAVSSEQLSASAEQTSSATEQTTSAIESIS----RG---ADMQTSAAQTSV 106 Query: 526 AANAEMDEAARGVGEASGQGAKQLEE----LLTQTSRTGEMTTALVDRVNNLKETV---- 577 EM++ + + GAKQ+E LT + + V+ + ++ E+V Sbjct: 107 KTLEEMNQIVTEMTD----GAKQIESNANLTLTYANEGDTLVKETVENMQSIDESVKGSD 162 Query: 578 ----------SSVIKVLDVMKNITQQTNILSLNATIXXXXXXXXXXXFMVVADEVRQLAD 627 ++++LDV+++I +QTN+L+LNA I F VVA+EVR+LA+ Sbjct: 163 QAIQTLSKRSEDIVEILDVIQSIAEQTNLLALNAAIEAARAGEHGKGFAVVAEEVRKLAE 222 Query: 628 QSKQSIAVVAGITDKIITEMNETVAVLSEVAPLFKQQMNSVKSTSEIFVSVQGQMDDFIS 687 +S QS + + D+++ E N +V + V + + TSE F S+ ++ Sbjct: 223 ESSQSTKKIHDLIDEMVKETNNSVEKMGLVKEEVSVGLKTAAQTSEKFHSI-------VA 275 Query: 688 SLESVTGAIDSLNH 701 S++ VT ++++N Sbjct: 276 SIQGVTSQVEAMNR 289 >BS_tlpA [NT] COG0840 Methyl-accepting chemotaxis protein Length = 83 Score = 83.6 bits (205), Expect = 1e-15, Method: Compositional matrix adjust. Identities = 0/404 (0%), Positives = 0/404 (0%), Gaps = 8/404 (1%) Query: 365 WKLAGIVPTGELVKAATPXXXXXXXXXXXXXXXXXXVGFVMVRMIARPLARLKDLMVEGA 424 Sbjct: 261 320 Query: 425 KGDLSVRTEYVSKDEIGELSASFNTMMERITELVAQTTDTAREVLETAGEL----GDASR 480 Sbjct: 321 380 Query: 481 XXXXXXXXXXXXXXXXXXXXXXLAQEAERGNELTDLIGTQMQSVIAANAEMDEAARGVGE 540 Sbjct: 381 ---- 436 Query: 541 ASGQGAKQLEELLTQTSRTGEMTTALVDRVNNLKETVSSVIKVLDVMKNITQQTNILSLN 600 Sbjct: 437 496 Query: 601 ATIXXXXXXXXXXXFMVVADEVRQLADQSKQSIAVVAGITDKIITEMNETVAVLSEVAPL 660 Sbjct: 497 556 Query: 661 FKQQMNSVKSTSEIFVSVQGQMDDFISSLESVTGAIDSLNHSQGVLSDAMSNXXXXXXXX 720 Sbjct: 557 616 Query: 721 XXTSEEVASLSNEQQNVSDQLVALSGKLEGASTQLKDKLSLFTI 764 Sbjct: 617 660 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.308 0.124 0.319 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 40,044,807 Number of extensions: 1339632 Number of successful extensions: 5038 Number of sequences better than 1.0e-05: 795 Number of HSP's gapped: 3725 Number of HSP's successfully gapped: 797 Length of query: 765 Length of database: 38,378,431 Length adjustment: 114 Effective length of query: 651 Effective length of database: 25,230,355 Effective search space: 16424961105 Effective search space used: 16424961105 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (21.7 bits) S2: 120 (50.8 bits)