BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0305.0010.p (441 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value BH1274 [O] COG0826 Collagenase and related proteases 517 e-146 BS_yrrO [O] COG0826 Collagenase and related proteases 514 e-146 SA1440 [O] COG0826 Collagenase and related proteases 487 e-137 lin0960 [O] COG0826 Collagenase and related proteases 440 e-123 SP1429 [O] COG0826 Collagenase and related proteases 401 e-111 >BH1274 [O] COG0826 Collagenase and related proteases Length = 420 Score = 517 bits (1331), Expect = e-146, Method: Compositional matrix adjust. Identities = 257/440 (58%), Positives = 304/440 (69%), Gaps = 22/440 (5%) Query: 1 MGTMTKPQFKGKRYRLDKPELLAPAGNLEKLKFAVHYGADAVYIGGQKYGLRSGADNFSF 60 M + +GKR KPELLAPAG+LEKLK A+HYGADAVYIGGQ++GLRS ADNFS Sbjct: 1 MQAIATKTAEGKRVITKKPELLAPAGSLEKLKVAIHYGADAVYIGGQEFGLRSNADNFSI 60 Query: 61 EEMREGVEFAKKYGAKVFVATNIYAHNEDIAGIEEYLRNLYEXXXXXXXXXXXXXXXXXR 120 EEMREGVEFA KYGAKV+V TNIYAHNE++ G+EEYL L E + Sbjct: 61 EEMREGVEFANKYGAKVYVTTNIYAHNENMDGLEEYLSALQEVGVTGIIVADPLIIETCK 120 Query: 121 RLVPGLEVHLSTQQSTLNWQAVSFWKEEGLPRVVLGRETSLEEIAEIKQHVDIEIESFIH 180 R+ P +EVHLSTQQS NW AV FWKEEGL RVVL RE LEE+ E+K+HVDIEIE+F+H Sbjct: 121 RVAPKVEVHLSTQQSLSNWLAVKFWKEEGLHRVVLAREVGLEEMLEMKKHVDIEIETFVH 180 Query: 181 GAMCSSYSGRCVLSNHFTDRDSNRGGCCQSCRWKYDLFEDARPEGTWVSEEDXXXXXXXX 240 GAMC SYSGRCVLSNH T RDSNRGGCCQSCRW YDL+E + E Sbjct: 181 GAMCISYSGRCVLSNHMTARDSNRGGCCQSCRWDYDLYEQQDSAEIPLFAEG-------- 232 Query: 241 XXXXXXXXXXXXXEDNPFSMGSKDLCMLESIPELIEAGIDSFKIEGRMKSIHYVATVVNA 300 D P++M KDL ++++IP+LIEAGIDS K+EGRMKSIHYVATV + Sbjct: 233 --------------DVPYTMSPKDLNLIQAIPQLIEAGIDSLKVEGRMKSIHYVATVTSV 278 Query: 301 YRKAIDAYMADPEGYVLKPEWLEELQKAANRPLNTGFFYDTPDHEDHIYEPEEKAAPYDF 360 YRK IDAY +DP+ + +K EWLEEL+K ANR FF TP +++ +Y K YDF Sbjct: 279 YRKVIDAYCSDPDNFKIKREWLEELEKCANRDFAPQFFEGTPTYKEQMYGIHPKRTKYDF 338 Query: 361 AGLVLEYDAAGGTALIQQRNHFKPGQEVEFFGPDNTFFKQTVGEIWDEEGQPLDAARHPL 420 GLVL+Y+ G +QQRNHFK G EVEFFGP+ F QTV +IWDE+G LDAARHPL Sbjct: 339 VGLVLDYNEKTGIVTLQQRNHFKQGDEVEFFGPEINRFTQTVEKIWDEDGNELDAARHPL 398 Query: 421 QRVRMKVDQPVAYFDMMRKK 440 Q V+ KVDQ V +MMRK+ Sbjct: 399 QIVKFKVDQKVYPQNMMRKE 418 >BS_yrrO [O] COG0826 Collagenase and related proteases Length = 422 Score = 514 bits (1325), Expect = e-146, Method: Compositional matrix adjust. Identities = 255/431 (59%), Positives = 299/431 (69%), Gaps = 22/431 (5%) Query: 11 GKRYRLDKPELLAPAGNLEKLKFAVHYGADAVYIGGQKYGLRSGADNFSFEEMREGVEFA 70 GKR KPELLAPAGNLEKLK AVHYGADAV+IGGQ+YGLRS ADNF+ EE+ EGVEFA Sbjct: 14 GKRVITKKPELLAPAGNLEKLKIAVHYGADAVFIGGQEYGLRSNADNFTIEEIAEGVEFA 73 Query: 71 KKYGAKVFVATNIYAHNEDIAGIEEYLRNLYEXXXXXXXXXXXXXXXXXRRLVPGLEVHL 130 KKYGAK++V TNI+AHNE++ G+E+YL+ L + RR+ P +EVHL Sbjct: 74 KKYGAKIYVTTNIFAHNENMDGLEDYLKALGDANVAGIIVADPLIIETCRRVAPNVEVHL 133 Query: 131 STQQSTLNWQAVSFWKEEGLPRVVLGRETSLEEIAEIKQHVDIEIESFIHGAMCSSYSGR 190 STQQS NW+AV FWKEEGL RVVL RETS EI E+K+ VDIEIESFIHGAMC +YSGR Sbjct: 134 STQQSLSNWKAVQFWKEEGLDRVVLARETSALEIREMKEKVDIEIESFIHGAMCIAYSGR 193 Query: 191 CVLSNHFTDRDSNRGGCCQSCRWKYDLFEDARPEGTWVSEEDXXXXXXXXXXXXXXXXXX 250 CVLSNH T RDSNRGGCCQSCRW YDL++ + E Sbjct: 194 CVLSNHMTARDSNRGGCCQSCRWDYDLYQTDGANAVALYGE------------------- 234 Query: 251 XXXEDNPFSMGSKDLCMLESIPELIEAGIDSFKIEGRMKSIHYVATVVNAYRKAIDAYMA 310 ED PF+M KDL ++ESIP++IE GIDS KIEGRMKSIHYVATVV+ YRK IDAY A Sbjct: 235 ---EDAPFAMSPKDLKLIESIPKMIEMGIDSLKIEGRMKSIHYVATVVSVYRKVIDAYCA 291 Query: 311 DPEGYVLKPEWLEELQKAANRPLNTGFFYDTPDHEDHIYEPEEKAAPYDFAGLVLEYDAA 370 DPE +V++ EWLEEL K ANR T FF TP +E+ ++ K YDF GLVL YD Sbjct: 292 DPENFVIQKEWLEELDKCANRDTATAFFEGTPGYEEQMFGEHAKKTTYDFVGLVLNYDED 351 Query: 371 GGTALIQQRNHFKPGQEVEFFGPDNTFFKQTVGEIWDEEGQPLDAARHPLQRVRMKVDQP 430 +QQRN FK G EVEFFGP+ F T+ IWDE+G LDAARHPLQ V+ K+D+ Sbjct: 352 TQMVTLQQRNFFKKGDEVEFFGPEIENFTHTIETIWDEDGNELDAARHPLQIVKFKLDKK 411 Query: 431 VAYFDMMRKKK 441 + +MMRK K Sbjct: 412 IYPSNMMRKGK 422 >SA1440 [O] COG0826 Collagenase and related proteases Length = 422 Score = 487 bits (1254), Expect = e-137, Method: Compositional matrix adjust. Identities = 238/426 (55%), Positives = 296/426 (69%), Gaps = 21/426 (4%) Query: 16 LDKPELLAPAGNLEKLKFAVHYGADAVYIGGQKYGLRSGADNFSFEEMREGVEFAKKYGA 75 + KPELLAPAGNLEKLK AVHYGADAV++GGQ+YGLRS ADNF+ EE+ EGVEFA +YGA Sbjct: 15 MKKPELLAPAGNLEKLKIAVHYGADAVFLGGQEYGLRSNADNFTMEEIAEGVEFANRYGA 74 Query: 76 KVFVATNIYAHNEDIAGIEEYLRNLYEXXXXXXXXXXXXXXXXXRRLVPGLEVHLSTQQS 135 K++V TNI AH+E+I G+E YLRNL + + + P LE+HLSTQQS Sbjct: 75 KIYVTTNIIAHDENIEGLESYLRNLEKTGATGIIVADPLIIETCKEVAPKLEIHLSTQQS 134 Query: 136 TLNWQAVSFWKEEGLPRVVLGRETSLEEIAEIKQHVDIEIESFIHGAMCSSYSGRCVLSN 195 N++AV +WKEEGL RVVL RET E+ E+K+ VDIEIE+FIHGAMC +YSGRC LSN Sbjct: 135 LSNYKAVEYWKEEGLDRVVLARETGAMEMREMKEKVDIEIEAFIHGAMCIAYSGRCTLSN 194 Query: 196 HFTDRDSNRGGCCQSCRWKYDLFEDARPEGTWVSEEDXXXXXXXXXXXXXXXXXXXXXED 255 H T RDSNRGGCCQSCRW Y+L E E Sbjct: 195 HMTARDSNRGGCCQSCRWDYELLE--------------------VDDNGELDVFYNQGEV 234 Query: 256 NPFSMGSKDLCMLESIPELIEAGIDSFKIEGRMKSIHYVATVVNAYRKAIDAYMADPEGY 315 PF+M KDL ++ESIP++++ G+DS KIEGRMKSIHY+ATVV+ YRK IDAY ADP+ + Sbjct: 235 TPFAMSPKDLKLIESIPQMMDIGVDSLKIEGRMKSIHYIATVVSVYRKVIDAYAADPDNF 294 Query: 316 VLKPEWLEELQKAANRPLNTGFFYDTPDHEDHIY-EPEEKAAPYDFAGLVLEYDAAGGTA 374 + PEWL EL K ANR FF TP +E+ ++ + + K +P+DF GLVL+Y+ A Sbjct: 295 KINPEWLIELDKCANRDTAPAFFEGTPGYEEQMFGQQQSKKSPFDFCGLVLDYNEDTKIA 354 Query: 375 LIQQRNHFKPGQEVEFFGPDNTFFKQTVGEIWDEEGQPLDAARHPLQRVRMKVDQPVAYF 434 IQQRN+FKPGQE+EFFGP+ F Q V I+DEEG LDAARHPLQ V++KVD+P+ Sbjct: 355 TIQQRNNFKPGQEIEFFGPEIETFTQVVEAIYDEEGNSLDAARHPLQIVQIKVDRPIYPN 414 Query: 435 DMMRKK 440 +MMRK+ Sbjct: 415 NMMRKE 420 >lin0960 [O] COG0826 Collagenase and related proteases Length = 408 Score = 440 bits (1131), Expect = e-123, Method: Compositional matrix adjust. Identities = 217/426 (50%), Positives = 278/426 (65%), Gaps = 22/426 (5%) Query: 16 LDKPELLAPAGNLEKLKFAVHYGADAVYIGGQKYGLRSGADNFSFEEMREGVEFAKKYGA 75 L KPE+LAPAGNLEKLK A+ YGADAVYIGGQ +GLRS A NFSF+EM+EG+ FA + A Sbjct: 5 LKKPEVLAPAGNLEKLKIAIRYGADAVYIGGQAFGLRSRAGNFSFDEMKEGIAFAHERNA 64 Query: 76 KVFVATNIYAHNEDIAGIEEYLRNLYEXXXXXXXXXXXXXXXXXRRLVPGLEVHLSTQQS 135 KV+VA N+ AH D G E+ R L + + PGL VHLSTQ S Sbjct: 65 KVYVAANMVAHAGDTEGAGEFFRTLRDIGIDAVIVSDPALISICFQDAPGLPVHLSTQAS 124 Query: 136 TLNWQAVSFWKEEGLPRVVLGRETSLEEIAEIKQHVDIEIESFIHGAMCSSYSGRCVLSN 195 N++ + FWK++GL RVVL RE S++EI EI + D+E+E+FIHGAMC SYSGRC LSN Sbjct: 125 ATNYKTLEFWKKQGLERVVLAREVSMQEIQEIGEKTDVEMEAFIHGAMCISYSGRCTLSN 184 Query: 196 HFTDRDSNRGGCCQSCRWKYDLFEDARPEGTWVSEEDXXXXXXXXXXXXXXXXXXXXXED 255 H +RD+NRGGC QSCRWKYDLFE + + D + Sbjct: 185 HMANRDANRGGCAQSCRWKYDLFEIDNGVSKNLVDTD----------------------E 222 Query: 256 NPFSMGSKDLCMLESIPELIEAGIDSFKIEGRMKSIHYVATVVNAYRKAIDAYMADPEGY 315 PFSM + DL M++ IP++++AG+DS KIEGRMKSIHYV+TV + YR+A+DAY ADPE Y Sbjct: 223 EPFSMSAVDLSMIKYIPDMVDAGVDSLKIEGRMKSIHYVSTVASVYRRAVDAYCADPENY 282 Query: 316 VLKPEWLEELQKAANRPLNTGFFYDTPDHEDHIYEPEEKAAPYDFAGLVLEYDAAGGTAL 375 P W EEL K A R L+TGFFY P ++ ++ K Y FA VLEYDA A Sbjct: 283 EFDPAWEEELWKVAQRELSTGFFYKEPTEDEQLFGKTRKIPQYAFAAQVLEYDAETKIAT 342 Query: 376 IQQRNHFKPGQEVEFFGPDNTFFKQTVGEIWDEEGQPLDAARHPLQRVRMKVDQPVAYFD 435 +QQRN+F G+E+EF+GP +T FKQ V +W+E+G+ +D A + + ++M VD+PV F Sbjct: 343 LQQRNNFGVGEEIEFYGPGDTGFKQVVEVLWNEDGEEIDRAPNAMMTIKMPVDRPVKPFY 402 Query: 436 MMRKKK 441 MRKKK Sbjct: 403 FMRKKK 408 >SP1429 [O] COG0826 Collagenase and related proteases Length = 428 Score = 401 bits (1030), Expect = e-111, Method: Compositional matrix adjust. Identities = 202/427 (47%), Positives = 266/427 (62%), Gaps = 22/427 (5%) Query: 16 LDKPELLAPAGNLEKLKFAVHYGADAVYIGGQKYGLRSGADNFSFEEMREGVEFAKKYGA 75 L +PE+L+PAG LEKLK AV YGADAV+IGGQ YGLRS A NF+FE+M EGV+FA KYGA Sbjct: 5 LKRPEVLSPAGTLEKLKVAVQYGADAVFIGGQAYGLRSRAGNFTFEQMEEGVQFAAKYGA 64 Query: 76 KVFVATNIYAHNEDIAGIEEYLRNLYEXXXXXXXXXXXXXXXXXRRLVPGLEVHLSTQQS 135 KV+VA N+ H + AG E+ R L + PGLE+HLSTQ S Sbjct: 65 KVYVAANMVMHEGNEAGAGEWFRKLRDIGIAAVIVSDPALIMIAVTEAPGLEIHLSTQAS 124 Query: 136 TLNWQAVSFWKEEGLPRVVLGRETSLEEIAEIKQHVDIEIESFIHGAMCSSYSGRCVLSN 195 N++ + FWKE GL RVVL RE S+EE+AEI++ D+EIE+F+HGAMC SYSGRC LSN Sbjct: 125 ATNYETLEFWKELGLTRVVLAREVSMEELAEIRKRTDVEIEAFVHGAMCISYSGRCTLSN 184 Query: 196 HFTDRDSNRGGCCQSCRWKYDLFEDARPEGTWVSEEDXXXXXXXXXXXXXXXXXXXXXED 255 H + RD+NRGGC QSCRWKYDL++ P G Sbjct: 185 HMSMRDANRGGCSQSCRWKYDLYD--MPFG-------------------KERKSLQGEIP 223 Query: 256 NPFSMGSKDLCMLESIPELIEAGIDSFKIEGRMKSIHYVATVVNAYRKAIDAYMADPEGY 315 FSM + D+ M++ IP++IE G+DS KIEGRMKSIHYV+TV N Y+ A+DAY+ PE + Sbjct: 224 EEFSMSAVDMSMIDHIPDMIENGVDSLKIEGRMKSIHYVSTVTNCYKAAVDAYLESPEKF 283 Query: 316 -VLKPEWLEELQKAANRPLNTGFFYDTPDHEDHIYEPEEKAAPYDFAGLVLEYDAAGGTA 374 +K + ++E+ K A R L TGF+Y TP + ++ K Y F V+ YD A TA Sbjct: 284 EAIKQDLVDEMWKVAQRELATGFYYGTPSENEQLFGARRKIPEYKFVAEVVSYDDAAQTA 343 Query: 375 LIQQRNHFKPGQEVEFFGPDNTFFKQTVGEIWDEEGQPLDAARHPLQRVRMKVDQPVAYF 434 I+QRN G +VEF+GP F+ + ++ D +G +D A +P++ + +KV QPV Sbjct: 344 TIRQRNVINEGDQVEFYGPGFRHFETYIEDLHDAKGNKIDRAPNPMELLTIKVPQPVQSG 403 Query: 435 DMMRKKK 441 DM+R K Sbjct: 404 DMVRALK 410 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.318 0.136 0.414 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 32,753,238 Number of extensions: 1373249 Number of successful extensions: 7978 Number of sequences better than 1.0e-05: 52 Number of HSP's gapped: 7870 Number of HSP's successfully gapped: 83 Length of query: 441 Length of database: 38,378,431 Length adjustment: 109 Effective length of query: 332 Effective length of database: 25,807,025 Effective search space: 8567932300 Effective search space used: 8567932300 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 117 (49.7 bits)