BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0305.0009.p (310 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value BH1273 [O] COG0826 Collagenase and related proteases 296 2e-80 BS_yrrN [O] COG0826 Collagenase and related proteases 295 6e-80 SA1441 [O] COG0826 Collagenase and related proteases 255 6e-68 CAC1687 [O] COG0826 Collagenase and related proteases 210 3e-54 CAC2974 [O] COG0826 Collagenase and related proteases 179 4e-45 >BH1273 [O] COG0826 Collagenase and related proteases Length = 309 Score = 296 bits (759), Expect = 2e-80, Method: Compositional matrix adjust. Identities = 144/288 (50%), Positives = 197/288 (68%), Gaps = 2/288 (0%) Query: 24 GADALLIGDDRFGMRLAGHFSLEDTAEAVKLAHEQGCKVYASLGGLMPNRLLDELPAYVK 83 GADA+LIG+ R+G+RLAG F+ +D +A +A E G KVY ++ + N +D L YV+ Sbjct: 23 GADAILIGEQRYGLRLAGEFTKQDVQKATVIAQELGAKVYVAMNAIFHNEKVDMLAEYVR 82 Query: 84 AIGELGVDGVEFGDPAVLAAVKREAPLLKLHWNAEMTSTNYATANYWGRKGASRVVLARE 143 + + G+DG+ FGDPAVL V+ AP +KLHWN E T+TN+ TANYWGRKGA R VLARE Sbjct: 83 FLRDTGIDGIVFGDPAVLMTVREVAPNMKLHWNTETTATNWYTANYWGRKGAKRAVLARE 142 Query: 144 LNMDEMTEMVPLLEVEAQVQVHGMTNIYHSKRKLVASYMSHQGRPSDGGSLGKERGLFLI 203 ++MD + E+ EVE +VQVHGMT ++ SKRKLV +YM QG+ + + K+R +FL Sbjct: 143 ISMDAIVEIKEHAEVEIEVQVHGMTAMFQSKRKLVGNYMEFQGKNLEVENRDKKRNMFLY 202 Query: 204 EAERPNEKFPIYEDENGTHIMSSDDICILEDLHFLLKAGVHSLKIEGLLKPVAYNAAVVK 263 + ER K+PI+EDENGTHIMS +DICI+++L ++ AG+ S KI+G+LK Y V Sbjct: 203 DPER-EAKYPIFEDENGTHIMSPNDICIIDELEDMIDAGIDSFKIDGILKTTEYMEKVTS 261 Query: 264 AYRHAIDLYAADPEGY-AFQESWMDGIRALQDPERELSFGFFYKEQVY 310 YR AIDL A D E Y +E +++ I A+Q R + GFF+KE VY Sbjct: 262 LYRQAIDLCADDREAYEEKKEDFIEEIEAIQPVNRSIDTGFFFKETVY 309 >BS_yrrN [O] COG0826 Collagenase and related proteases Length = 309 Score = 295 bits (755), Expect = 6e-80, Method: Compositional matrix adjust. Identities = 145/288 (50%), Positives = 196/288 (68%), Gaps = 2/288 (0%) Query: 24 GADALLIGDDRFGMRLAGHFSLEDTAEAVKLAHEQGCKVYASLGGLMPNRLLDELPAYVK 83 GA A L+G+ R+G+RLAG FS ED +AV++AH++G KVY ++ + N + EL Y+ Sbjct: 23 GATAFLVGEQRYGLRLAGEFSREDVTKAVEIAHKEGAKVYVAVNAIFHNDKVGELGEYLA 82 Query: 84 AIGELGVDGVEFGDPAVLAAVKREAPLLKLHWNAEMTSTNYATANYWGRKGASRVVLARE 143 + E GVD FGDPAVL A + AP LKLHW+ E T TNY T NYWGRKGA+R VLARE Sbjct: 83 FLAEAGVDAAVFGDPAVLMAARESAPDLKLHWSTETTGTNYYTCNYWGRKGAARSVLARE 142 Query: 144 LNMDEMTEMVPLLEVEAQVQVHGMTNIYHSKRKLVASYMSHQGRPSDGGSLGKERGLFLI 203 LNMD + E+ EVE ++QVHGMT ++ SKR L+ +Y +QG+ D KE G+FL Sbjct: 143 LNMDSIVEIKENAEVEIEIQVHGMTCMFQSKRSLIGNYFEYQGKVMDIERKKKESGMFLH 202 Query: 204 EAERPNEKFPIYEDENGTHIMSSDDICILEDLHFLLKAGVHSLKIEGLLKPVAYNAAVVK 263 + ER N K+PI+EDENGTHIMS +D+CI+++L L+ AG+ S KI+G+LK Y V K Sbjct: 203 DKERDN-KYPIFEDENGTHIMSPNDVCIIDELEELIDAGIDSFKIDGVLKMPEYLIEVTK 261 Query: 264 AYRHAIDLYAADPEGY-AFQESWMDGIRALQDPERELSFGFFYKEQVY 310 YR AIDL + + Y A +E W++ I ++Q R++ GFF+KE VY Sbjct: 262 MYREAIDLCVENRDEYEAKKEDWIERIESIQPVNRKIDTGFFFKETVY 309 >SA1441 [O] COG0826 Collagenase and related proteases Length = 307 Score = 255 bits (652), Expect = 6e-68, Method: Compositional matrix adjust. Identities = 133/289 (46%), Positives = 179/289 (61%), Gaps = 4/289 (1%) Query: 24 GADALLIGDDRFGMRLAGHFSLEDTAEAVKLAHEQGCKVYASLGGLMPNRLLDELPAYVK 83 GADA +IG+ +FG+RL G F+ + EAV LAH+ KVYA++ GL N LD + Y+ Sbjct: 21 GADAFVIGEQKFGLRLPGEFNRQQMTEAVALAHKNDKKVYAAVNGLFHNYHLDAVEDYIN 80 Query: 84 AIGELGVDGVEFGDPAVLAAVKREAPLLKLHWNAEMTSTNYATANYWGRKGASRVVLARE 143 + E+ VD + FGDPAV+ VK + + LHWNAE TN+ NYWG++GASR VLARE Sbjct: 81 FLHEIRVDRIIFGDPAVVMYVKAQENPIPLHWNAETLVTNHFQCNYWGKRGASRAVLARE 140 Query: 144 LNMDEMTEMVPLLEVEAQVQVHGMTNIYHSKRKLVASYMSHQGRPSDGGSLGKERGLFLI 203 LN+DE+ + VE +VQV GMT ++ SKR L+ +Y + Q R E+ L L Sbjct: 141 LNLDEIINIKENSNVEIEVQVQGMTCMFQSKRMLLGNYYTFQDRQMKIERRNDEQSLLLY 200 Query: 204 EAERPNEKFPIYEDENGTHIMSSDDICILEDLHFLLKAGVHSLKIEGLLKPVAYNAAVVK 263 + ER N +P+YED NGTHIMS +DIC++E+L +AG+ S KI+G+L+ Y V + Sbjct: 201 DEERQN-NYPVYEDYNGTHIMSPNDICLIEELAPFFEAGIDSFKIDGILQTEEYINVVTE 259 Query: 264 AYRHAIDLYAADPEGYAFQESWM--DGIRALQDPERELSFGFFYKEQVY 310 YR AIDLY DPE Y E +M D I +Q R GF YK+ VY Sbjct: 260 QYRQAIDLYNEDPEIYE-DEKFMLIDPIEEIQPDHRPFDEGFLYKQTVY 307 >CAC1687 [O] COG0826 Collagenase and related proteases Length = 406 Score = 210 bits (534), Expect = 3e-54, Method: Compositional matrix adjust. Identities = 116/289 (40%), Positives = 174/289 (60%), Gaps = 13/289 (4%) Query: 24 GADALLIGDDRFGMR-LAGHFSLEDTAEAVKLAHEQGCKVYASLGGLMPNRLLDELPAYV 82 GADA+ +G + +R A +FSL++ E ++ AH + KVY +L N L+ L Y+ Sbjct: 23 GADAVYLGGSKLNLRAFADNFSLDELKEGIEFAHSRNKKVYITLNVFPHNEDLNGLEEYL 82 Query: 83 KAIGELGVDGVEFGDPAVLAAVKREAPLLKLHWNAEMTSTNYATANYWGRKGASRVVLAR 142 K + E+ D V DP ++ + P L++H + + S NY TA +W ++G R+V+AR Sbjct: 83 KDLYEIKADAVIVSDPGIIMTAREVVPNLEIHLSTQANSVNYKTAIFWHKQGVKRIVVAR 142 Query: 143 ELNMDE---MTEMVP-LLEVEAQVQVHGMTNIYHSKRKLVASYMSHQGRPSDGGSLGK-- 196 EL++ E M E +P E+EA V HG I +S R L+++YM+ GR S+ G+ + Sbjct: 143 ELSLKEIKSMKEKIPETCEIEAFV--HGSMCISYSGRCLLSNYMT--GRDSNRGACAQPC 198 Query: 197 ERGLFLIEAERPNEKFPIYEDENGTHIMSSDDICILEDLHFLLKAGVHSLKIEGLLKPVA 256 L+E +RPNE FP+YEDE GT+IM+S D+C++E + L+ AG+ SLKIEG +K Sbjct: 199 RYKYSLVEEKRPNEYFPVYEDEKGTYIMNSKDLCMIEHIPDLISAGIDSLKIEGRMKSSY 258 Query: 257 YNAAVVKAYRHAIDLYAADPEGYAFQESWMDGIRALQDPERELSFGFFY 305 Y A+VVKAYR AID Y +PE Y F E WM+ + + R + GF+Y Sbjct: 259 YVASVVKAYREAIDTYFENPEEYKFNEKWMEYL--TKPSHRRYTTGFYY 305 >CAC2974 [O] COG0826 Collagenase and related proteases Length = 406 Score = 179 bits (455), Expect = 4e-45, Method: Compositional matrix adjust. Identities = 98/291 (33%), Positives = 171/291 (58%), Gaps = 10/291 (3%) Query: 24 GADALLIGDDRFGMR-LAGHFSLEDTAEAVKLAHEQGCKVYASLGGLMPNRLLDELPAYV 82 GADA+ +G ++ +R A +F++E E +K AH++G KVY ++ ++ L L Y+ Sbjct: 23 GADAVYLGGNKLNLRAYADNFTIEQLKEGIKFAHDRGKKVYITVNIFPHDKDLVGLEEYL 82 Query: 83 KAIGELGVDGVEFGDPAVLAAVKREAPLLKLHWNAEMTSTNYATANYWGRKGASRVVLAR 142 K + E+ D + DP ++A K P L++H + + TNY +A +W + G RVV+AR Sbjct: 83 KELDEIDADAILAADPGIIAVAKEVVPNLEIHLSTQANCTNYKSALFWHKLGVKRVVMAR 142 Query: 143 ELNMDEMTEMVPLLEVEAQVQ--VHGMTNIYHSKRKLVASYMSHQGRPSDGGSLGK--ER 198 EL++++ ++ L +++ VHG + +S R +++YM+ GR ++ G + Sbjct: 143 ELSLNDFKKLREELPKSCEIEAFVHGSMCMAYSGRCALSNYMT--GRDANRGECSQPCRY 200 Query: 199 GLFLIEAERPNEKFPIYEDENGTHIMSSDDICILEDLHFLLKAGVHSLKIEGLLKPVAYN 258 L E E+ +K I E+ GT+IM+S D+C++E + L+ +G++SLKIEG +K Y Sbjct: 201 KYHLFEQEKGEQK-DITENGKGTYIMNSKDLCMIEHIPELISSGINSLKIEGRMKSAFYV 259 Query: 259 AAVVKAYRHAIDLYAADPEGYAFQESWMDGIRALQDPERELSFGFFYKEQV 309 A+VVKAYR A+D Y +P+ Y F WM+ ++ + R + GF++ E+V Sbjct: 260 ASVVKAYRQAVDSYVENPDKYVFNPKWMENLK--KPSHRPYTTGFYFGEEV 308 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.318 0.136 0.401 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 22,157,747 Number of extensions: 968671 Number of successful extensions: 2276 Number of sequences better than 1.0e-05: 52 Number of HSP's gapped: 2207 Number of HSP's successfully gapped: 60 Length of query: 310 Length of database: 38,378,431 Length adjustment: 106 Effective length of query: 204 Effective length of database: 26,153,027 Effective search space: 5335217508 Effective search space used: 5335217508 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 116 (49.3 bits)