BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0305.0003.p (878 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value PH1969 [R] COG2872 Predicted metal-dependent hydrolases related ... 85 5e-16 PAB0066 [R] COG2872 Predicted metal-dependent hydrolases related... 84 8e-16 PH0108 [R] COG2872 Predicted metal-dependent hydrolases related ... 84 1e-15 Ta0124m [R] COG2872 Predicted metal-dependent hydrolases related... 62 4e-09 SSO0450 [R] COG2872 Predicted metal-dependent hydrolases related... 56 2e-07 >PH1969 [R] COG2872 Predicted metal-dependent hydrolases related to alanyl-tRNA synthetase HxxxH domain Length = 346 Score = 85.1 bits (209), Expect = 5e-16, Method: Compositional matrix adjust. Identities = 26/342 (7%), Positives = 57/342 (16%), Gaps = 25/342 (7%) Query: 475 VDGELAETAGEGTDCQVILESTPFYAESGGQVSDTGLLTGGSVTAKVTGLFKAPHGQHVH 534 G T GE + G + D + + GL + + + Sbjct: 15 TLGLGTMTFGEQNSEADAHAQLDYAVAQGINLIDVAEMYPVPPRPETQGLTET-YVGNWL 73 Query: 535 LVTVEAGELRVGDSVRAEVNREQREDIVKNHTATHLLHKALKEVLGGHVNQAGSLVEGAR 594 +L + V + + +AL + L L + Sbjct: 74 AKHGSREKLIIASKVSGPSRNNDKGIRPDQALDRKNIREALHDSLKRLQTDYLDLYQVHW 133 Query: 595 LRFDFSHFGAITPEELSDIEHRVNAQIWRGLDVVIENKPIDEAKAMGAMALFGE--KYGN 652 + + A + A + + + E Sbjct: 134 PQRP----TNCFGKLGYSWTDSAPAVSLLDTLDALAE--YQRAGKIRYIGVSNETAFGVM 187 Query: 653 IVRVVQVGDYSLELCGGIHVLNTSQIGIFKLVSESGIGSGVRRIEAVTG-RYAYQFTE-- 709 + + + + ++E GV + Sbjct: 188 RYLHLADKHDLPRIVTIQNPYSLLNRSFEVGLAEVSQYEGVELLAYSCLGFGTLTGKYLN 247 Query: 710 ----SQLDLLKQSAGLLKSSLNDVPKRIEALHAQVRELSRENESLQSMLSATFAAELTSS 765 + S S + R A Sbjct: 248 GAKPAGARNTLFSRFTRYSGEQTQKAVAAYVDIARRHGLDP------AQMALAFVRRQPF 301 Query: 766 VKTVGSGTQLLAVAVQAGNMDALRSTADELKSKLPDAVLVLG 807 V + Q + Sbjct: 302 VAS---TLLGATTMDQLKTNIESLHLELSEDVLAEIEAVHQV 340 >PAB0066 [R] COG2872 Predicted metal-dependent hydrolases related to alanyl-tRNA synthetase HxxxH domain Length = 346 Score = 84.3 bits (207), Expect = 8e-16, Method: Compositional matrix adjust. Identities = 6/214 (2%), Positives = 29/214 (13%), Gaps = 26/214 (12%) Query: 485 EGTDCQVILESTPFYAESGGQVSDTGLLTGGSVTAKVTGLFKAPHGQHVHLVTVEAGELR 544 Q + + + + Sbjct: 21 MTFGEQNSEADAHAQLDYAVAQGINLIDVA----EMYPVPPRPETQGLTETYV-GNWLAK 75 Query: 545 VGDSVRAEVNREQREDIVKNHTATHLLHKALKEVLG-GHVNQAGSLVEGARLRFDFSHFG 603 G + + + N ++ + + L + Sbjct: 76 HGSREKLIIASKVSGPSRNNDKGIRPDQALDRKNIREALHDSLKRLQTDYLDLYQVHW-- 133 Query: 604 AITPEELSDIEHRVNAQIWRGLDVVIENKPIDEAKAMGAMALFGEKYGNIVRVVQVGDYS 663 P+ ++ ++ + Sbjct: 134 ---PQRPTNCFGKL---------GYSWTDSAPAV---SLLDTLDA---LAEYQRAGKIRY 175 Query: 664 LELCGGIHVLNTSQIGIFKLVSESGIGSGVRRIE 697 + + + + I + Sbjct: 176 IGVSNETAFGVMRYLHLADKHDLPRIVTIQNPYS 209 >PH0108 [R] COG2872 Predicted metal-dependent hydrolases related to alanyl-tRNA synthetase HxxxH domain Length = 346 Score = 83.6 bits (205), Expect = 1e-15, Method: Composition-based stats. Identities = 11/226 (4%), Positives = 28/226 (12%), Gaps = 29/226 (12%) Query: 476 DGELAETAG---EGTDCQVILESTPFYAESGGQVSDTGLLTGGSVTAKVTGLFKAPHGQH 532 + + + + YA + G + Sbjct: 12 EVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGINLIDVAEMY----PVPPRPETQGLTET 67 Query: 533 VHLVTVEAGELRVGDSVRAEVNREQREDIVKNHTATHLLHKALKEVLG-GHVNQAGSLVE 591 A + A + K L + + + + Sbjct: 68 YVGNW-LAKHGSREKLIIASKVSGPSRNNDKGIRPDQALDRKNIREALHDSLKRLQTDYL 126 Query: 592 GARLRFDFSHFGAITPEELSDIEHRVNAQIWRGLDVVIENKPIDEAKAMGAMALFGEKYG 651 P + + E Sbjct: 127 DLYQVHWPQR-----PTNCFGKLGYS---------WTDSAPAVSLL---DTLDALAE--- 166 Query: 652 NIVRVVQVGDYSLELCGGIHVLNTSQIGIFKLVSESGIGSGVRRIE 697 + L I + + Sbjct: 167 YQRAGKIRYIGVSNETAFGVMRYLHLADKHDLPRIVTIQNPYSLLN 212 >Ta0124m [R] COG2872 Predicted metal-dependent hydrolases related to alanyl-tRNA synthetase HxxxH domain Length = 346 Score = 62.0 bits (149), Expect = 4e-09, Method: Compositional matrix adjust. Identities = 13/212 (6%), Positives = 35/212 (16%), Gaps = 8/212 (3%) Query: 491 VILESTPFYAESGGQVSDTGLLTGGSVTAKVTGLFKAPHGQHVHLVTVEAGELRVGDSVR 550 + + + ++ A + Sbjct: 27 NSEADAHAQLDYAVAQGINLIDVAEMYPVPPRPETQ--GLTETYVGNWLAKHGSREKLII 84 Query: 551 AEVNREQREDIVKNHTATHLLHKALKEVLGGHVNQAGSLVEGARLRFDFSHFGAITPEEL 610 A + K L + + + + Sbjct: 85 ASKVSGPSRNNDKGIRPDQALDRKNIREALHDSLKRLQTDYLDLYQVHWP-QRPTNCFGK 143 Query: 611 SDIEHRVNAQIWRGLDVVIENKPIDEAKAMGAMALF--GEKYGN---IVRVVQVGDYSLE 665 +A LD + A + + + + + Sbjct: 144 LGYSWTDSAPAVSLLDTLDALAEYQRAGKIRYIGVSNETAFGVMRYLHLADKHDLPRIVT 203 Query: 666 LCGGIHVLNTSQIGIFKLVSESGIGSGVRRIE 697 + +LN S VS+ + Sbjct: 204 IQNPYSLLNRSFEVGLAEVSQYEGVELLAYSC 235 >SSO0450 [R] COG2872 Predicted metal-dependent hydrolases related to alanyl-tRNA synthetase HxxxH domain Length = 346 Score = 56.2 bits (134), Expect = 2e-07, Method: Compositional matrix adjust. Identities = 7/215 (3%), Positives = 30/215 (13%), Gaps = 20/215 (9%) Query: 491 VILESTPFYAESGGQVSDTGLLTG-GSVTAKVTGLFKAPHGQHVHLVTVEAGELRVGDSV 549 + + + + + G Sbjct: 27 NSEADAHAQLDYAVAQGINLIDVAEMYPVPPRPETQGLTETYVGNWLA-KHGSREKLIIA 85 Query: 550 RAEVNREQREDIVKNHTATHLL-----HKALKEVLGGHVNQAGSLVEGARLRFDFSHFGA 604 + D + ++ + Sbjct: 86 SKVSGPSRNNDKGIRPDQALDRKNIREALHDSLKRLQTDYL-----DLYQVHWPQRP--- 137 Query: 605 ITPEELSDIEHRVNAQIWRGLDVVIENKPIDEAKAMGAMA-LFGEKYGNIV--RVVQVGD 661 + + + + + +A + V + Sbjct: 138 --TNCFGKLGYSWTDSAPAVSLLDTLDALAEYQRAGKIRYIGVSNETAFGVMRYLHLADK 195 Query: 662 YSLELCGGIHVLNTSQIGIFKLVSESGIGSGVRRI 696 + L I + F++ + Sbjct: 196 HDLPRIVTIQNPYSLLNRSFEVGLAEVSQYEGVEL 230 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.316 0.135 0.390 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 61,076,885 Number of extensions: 2700204 Number of successful extensions: 7405 Number of sequences better than 1.0e-05: 8 Number of HSP's gapped: 7393 Number of HSP's successfully gapped: 8 Length of query: 878 Length of database: 38,378,431 Length adjustment: 115 Effective length of query: 763 Effective length of database: 25,115,021 Effective search space: 19162761023 Effective search space used: 19162761023 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 120 (50.8 bits)