BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0303.0018.p (367 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value BH1086 [G] COG0448 ADP-glucose pyrophosphorylase 398 e-111 CAC2238 [G] COG0448 ADP-glucose pyrophosphorylase 273 4e-73 BS_glgD [G] COG0448 ADP-glucose pyrophosphorylase 243 3e-64 TM0239 [G] COG0448 ADP-glucose pyrophosphorylase 238 1e-62 FN0854 [G] COG0448 ADP-glucose pyrophosphorylase 218 1e-56 >BH1086 [G] COG0448 ADP-glucose pyrophosphorylase Length = 368 Score = 398 bits (1023), Expect = e-111, Method: Compositional matrix adjust. Identities = 187/364 (51%), Positives = 264/364 (72%), Gaps = 1/364 (0%) Query: 1 MKELMGVINLDHELDQLNELTYFRCGAAVPFASRYRLIDFVLSNMMRAELESVGLFVRRK 60 MK LMGV+NLDH+ D L+ELTYFR A VPF RYR+IDFVLSNM+ + ++ + +F R K Sbjct: 1 MKSLMGVVNLDHDQDLLHELTYFRSVAGVPFGGRYRMIDFVLSNMVNSGIQDIAVFARNK 60 Query: 61 YRSLMDHLGDGKSWDMNRKHGGLFILPPDWNDPTDTSLGDLQHYHNNLDFFKRASAKYIV 120 YRSLMDHLG G WD+NRK GGLFILPPDWNDPTD S GDL+H+HN+ DFF R +A +++ Sbjct: 61 YRSLMDHLGTGAPWDLNRKRGGLFILPPDWNDPTDISKGDLRHFHNHRDFFHRGNADHVL 120 Query: 121 FSGSQHINTADLQEVYQYHLEKGADVTLVYKKIEELQPEHDPCLRVEVDEDSNVTEIHQE 180 SGSQ I+ D ++ ++ H E ADVT++YKK+++L+PE +++E D NV I + Sbjct: 121 VSGSQFISKVDYEDAFKRHQETKADVTVLYKKVDQLEPEDRYRMKLEFDSFGNVKSITND 180 Query: 181 KDHPNVYLDIFIMEKKLFLEQVEYCIAHGESYFFRDAIQKNRHKFKIAAYEYTGYHAVIN 240 + + N+Y+ ++I+ K+L LE + +CI + + F D I N ++ I AYEY GY ++I+ Sbjct: 181 EMNSNLYMQMYIIRKELLLELLNHCIGYHKQDLFVDGIMSNLNELAIRAYEYKGYLSIIS 240 Query: 241 SLESYYKNSLNLLQQDNYFGLF-KENPVQTKIKYEAPTRYLDSAEVSNSLVANGCIIAGT 299 S+ SYYK+S++LL+ + Y LF +P+ TKIK E P +Y++ +EVSNSLVANGC+I G Sbjct: 241 SVASYYKHSMDLLKPEIYQSLFLAGSPIYTKIKDEPPAKYVNGSEVSNSLVANGCVIEGH 300 Query: 300 VENSVIFRGVQIRKGARIVNSVIMQKCVIEENAVIENVIMDKDVHLSRERILVGDSKRPF 359 VENS++FRGV + KG I NS+IMQ+C I E+ +++VI+DKD+ +S R G ++PF Sbjct: 301 VENSILFRGVYVAKGVSIKNSIIMQRCYIHEDTHLDHVILDKDILISPNRQFAGAPEQPF 360 Query: 360 VIAK 363 VIAK Sbjct: 361 VIAK 364 >CAC2238 [G] COG0448 ADP-glucose pyrophosphorylase Length = 372 Score = 273 bits (697), Expect = 4e-73, Method: Compositional matrix adjust. Identities = 143/365 (39%), Positives = 226/365 (61%), Gaps = 7/365 (1%) Query: 5 MGVINLDHELDQLNELTYFRCGAAVPFASRYRLIDFVLSNMMRAELESVGLFVRRKYRSL 64 M +++L+ + + LT R A++P A RYR+IDF+LSN++ + ++++G+ + K RSL Sbjct: 6 MSILSLNENDNDIKSLTKARPLASIPIAGRYRIIDFILSNLVNSGVKNIGILTQCKSRSL 65 Query: 65 MDHLGDGKSWDMNRKHGGLFILPPDWNDPTDTSLGDLQHYHNNLDFFKRASAKYIVFSGS 124 +DHLG GK WD++RK GLFI +++ T T L D++ +N+++ R++ + ++ S S Sbjct: 66 LDHLGSGKPWDLDRKLNGLFIF--NFSGITST-LSDIEMLKSNMEYLYRSTQEKVILSSS 122 Query: 125 QHINTADLQEVYQYHLEKGADVTLVYKKIEELQPEHDPCLRVEVDEDSNVTEIHQEK--- 181 I D Q ++H + G D+TL+YK+ + + C + +D+++NV I + Sbjct: 123 AVICNIDFQRAAEFHEKSGKDITLIYKRTNNGKRKFLNCSVLNMDKNNNVISIGKNTGLD 182 Query: 182 DHPNVYLDIFIMEKKLFLEQVEYCIAHGESYFFRDAIQKNRHKFKIAAYEYTGYHAVINS 241 D N+ L +FIM KKL + + CI G S + I+K+ + F + AYE+ GY I+S Sbjct: 183 DVQNISLGMFIMNKKLLINILNNCIRTGFSLSLKQCIRKHMNNFTVNAYEFNGYAEYIDS 242 Query: 242 LESYYKNSLNLLQQD-NYFGLFKENPVQTKIKYEAPTRYLDSAEVSNSLVANGCIIAGTV 300 ++SYYK ++++L D N FK + TK+K EAPT+Y + +EV NSL+ANG +I G + Sbjct: 243 VKSYYKVNMDMLNLDINREVFFKNGLIYTKVKDEAPTKYFNDSEVCNSLIANGSLIHGQI 302 Query: 301 ENSVIFRGVQIRKGARIVNSVIMQKCVIEENAVIENVIMDKDVHLSRERILVGDSKRPFV 360 ENS+I R V I KGARI N VIMQ C I+EN + NVI+DK+V + +L G + P V Sbjct: 303 ENSIIGRRVTISKGARIKNCVIMQNCTIKENVDLTNVIVDKNVIIDENTVLKGGKEFPLV 362 Query: 361 IAKSS 365 I K S Sbjct: 363 IEKKS 367 >BS_glgD [G] COG0448 ADP-glucose pyrophosphorylase Length = 343 Score = 243 bits (621), Expect = 3e-64, Method: Compositional matrix adjust. Identities = 136/366 (37%), Positives = 203/366 (55%), Gaps = 40/366 (10%) Query: 3 ELMGVINLDHELDQLNELTYFRCGAAVPFASRYRLIDFVLSNMMRAELESVGLFVRRKYR 62 +++GVI+ L +LT R A+PFA RYRLIDF+LSNM+ A++ SV +F + +YR Sbjct: 5 QMLGVIDETTYKHSLQDLTAQRSLGAIPFAGRYRLIDFMLSNMVNADIRSVAIFPKYRYR 64 Query: 63 SLMDHLGDGKSWDMNRKHGGLFILP-PDWNDPTDTSLGDLQHYHNNLDFFKRASAKYIVF 121 SLMDHLG GK WD++RK GLF P P + D G + + ++LD+F R++ +Y V Sbjct: 65 SLMDHLGAGKEWDLHRKKDGLFFFPSPHLHHEYD-EFGSFRQFSDHLDYFHRSTQQYAVI 123 Query: 122 SGSQHINTADLQEVYQYHLEKGADVTLVYKKIEELQPEHDPCLRVEVDEDSNVTEIHQEK 181 S S + Q V + H E G DVT V++ + LQ Sbjct: 124 SNSHTVCNIQFQYVLKRHQEVGCDVTEVFQDGQSLQ------------------------ 159 Query: 182 DHPNVYLDIFIMEKKLFLEQVEYCIAHGESYF--FRDAIQKNRHKFKIAAYEYTGYHAVI 239 I+IM L + + H E + ++A++K I YEY+GY AVI Sbjct: 160 --------IYIMSTTLLKDLI---YGHSEKGYKTIQEAVEKESSALTICPYEYSGYAAVI 208 Query: 240 NSLESYYKNSLNLLQQDNYFGLF-KENPVQTKIKYEAPTRYLDSAEVSNSLVANGCIIAG 298 +S+E YY +S+ L+Q + +F + P+ TK+K E PT+Y + V NSLVANGC++ G Sbjct: 209 DSVEKYYTHSMELIQPRFWQQVFLPQQPIYTKVKDEPPTKYGKHSTVKNSLVANGCVLEG 268 Query: 299 TVENSVIFRGVQIRKGARIVNSVIMQKCVIEENAVIENVIMDKDVHLSRERILVGDSKRP 358 VEN ++FR V + KG ++ N +IMQK I E+ ++E VI DKDV + + G +++P Sbjct: 269 EVENCILFRAVHVGKGTKLKNCIIMQKTQIGEDCLLEQVISDKDVKIGKATEAAGTAEQP 328 Query: 359 FVIAKS 364 V+ K Sbjct: 329 LVLRKG 334 >TM0239 [G] COG0448 ADP-glucose pyrophosphorylase Length = 370 Score = 238 bits (606), Expect = 1e-62, Method: Compositional matrix adjust. Identities = 133/362 (36%), Positives = 211/362 (58%), Gaps = 9/362 (2%) Query: 4 LMGVINLDHELDQLNELTYFRCGAAVPFASRYRLIDFVLSNMMRAELESVGLFVRRKYRS 63 ++G+I + D+L LT R AAVP +YR IDF LSNM+ + + VG+ + RS Sbjct: 3 VLGLILAGGKSDRLWPLTKVRASAAVPVFGKYRAIDFTLSNMVNSGIRKVGILTQYNPRS 62 Query: 64 LMDHLGDGKSWDMNRKHGGLFILPPDWNDPTDTSL--GDLQHYHNNLDFFKRASAKYIVF 121 LMDHLG GK WD++RK GGLFIL P + P G N+ +R +++ Sbjct: 63 LMDHLGSGKEWDLDRKSGGLFILQP-YVGPNREVWYRGTADAIFQNMTILRRGEEDHVLI 121 Query: 122 SGSQHINTADLQEVYQYHLEKGADVTLVYKKIEELQPEHDPCLRVEVDEDSNVTEIHQEK 181 HI ++++ YHL+KGAD+TLV K+++E + + V++D+D V EI ++ Sbjct: 122 GSGDHIYKMIYRDLFNYHLKKGADITLVVKELDETYNLSEYGI-VQLDDDMRVVEIEEKP 180 Query: 182 DHPN---VYLDIFIMEKKLFLEQVEYCIAHGESYFFRDAIQKNRHKFKIAAYEYTGY-HA 237 HP +L ++ M K+L E + + G+ D + N + K+ AY + GY Sbjct: 181 AHPKGNIAFLGVYFMNKELLKELLYATVPQGKYDLLLDIVIPNLDRLKVYAYRFDGYWRN 240 Query: 238 VINSLESYYKNSLNLLQQDNYFGLFKEN-PVQTKIKYEAPTRYLDSAEVSNSLVANGCII 296 V ++ YY+ ++++L+++ LF N V TK+K P ++ +A V NSL+A+G I+ Sbjct: 241 VKKGIKEYYRINMDILKKEVRDELFYRNGKVYTKLKDLPPPKFTTTAVVENSLIADGSIV 300 Query: 297 AGTVENSVIFRGVQIRKGARIVNSVIMQKCVIEENAVIENVIMDKDVHLSRERILVGDSK 356 +GTV NSVIFR V+I+ GA + NS+IM+ V+EE AV+ NVI+DK+ + R++ GD++ Sbjct: 301 SGTVRNSVIFRNVRIKVGAVVENSIIMENTVVEEGAVLRNVILDKNCIVREGRVIEGDTE 360 Query: 357 RP 358 P Sbjct: 361 LP 362 >FN0854 [G] COG0448 ADP-glucose pyrophosphorylase Length = 387 Score = 218 bits (556), Expect = 1e-56, Method: Compositional matrix adjust. Identities = 127/373 (34%), Positives = 205/373 (54%), Gaps = 10/373 (2%) Query: 1 MKELMGVINLDHELDQLNELTYFRCGAAVPFASRYRLIDFVLSNMMRAELESVGLFV-RR 59 ++ M +I L ++ LT R A++P YR+IDF LSN++ A + +VGLF Sbjct: 2 IRSYMAIIYLGDSKKNISPLTKVRALASIPVGGSYRIIDFSLSNVVNAGIRNVGLFCGNE 61 Query: 60 KYRSLMDHLGDGKSWDMNRKHGGLFILPPDWNDPTDTSLGDLQHYHNNLDFFKRASAKYI 119 + SL DH+G G WD+ RK G+FI +D + + N+++F R++ + I Sbjct: 62 ELNSLTDHIGMGAEWDLARKKDGIFIFKRMLDDNFSLNQARISK---NMEYFFRSTQQNI 118 Query: 120 VFSGSQHINTADLQEVYQYHLEKGADVTLVYKKIEELQPEHDPCLRVEVDEDSNVTEIHQ 179 V + + D+ ++ + H G ++T+VYKK+++ + C V++DE++ V I Q Sbjct: 119 VVLNAHMVYNLDVSDLIEKHEASGKEITMVYKKVKKANEHFNHCSSVKIDENNRVIGIGQ 178 Query: 180 E---KDHPNVYLDIFIMEKKLFLEQVEYCIAHGESYFFRDAIQKNRHKFKIAAYEYTGYH 236 K+ N+ LD F++ K+L L+ + I G+ + I +N I AYE+ GY Sbjct: 179 NLFFKEEENISLDAFVLSKELMLKLLVDSIQEGKYNVLSELIARNLPSLNINAYEFKGYL 238 Query: 237 AVINSLESYYKNSLNLLQQ---DNYFGLFKENPVQTKIKYEAPTRYLDSAEVSNSLVANG 293 INS Y+ ++NLL Q D+ FG+ + TK+K PT + ++A+V NSLV+NG Sbjct: 239 QCINSTREYFNFNMNLLNQKIRDDVFGIKSGRKIFTKVKDTPPTLFKETADVENSLVSNG 298 Query: 294 CIIAGTVENSVIFRGVQIRKGARIVNSVIMQKCVIEENAVIENVIMDKDVHLSRERILVG 353 CII GTV+NS++ RG I K + VI+Q C I++ A ++NVI+DK+ + L Sbjct: 299 CIIEGTVKNSILSRGAIIEKDVVLEECVILQDCHIKKGAHLKNVIVDKNNIIHENEKLSA 358 Query: 354 DSKRPFVIAKSSK 366 + P VI KS K Sbjct: 359 SKEYPLVIEKSMK 371 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.321 0.137 0.401 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 26,656,362 Number of extensions: 1034545 Number of successful extensions: 1907 Number of sequences better than 1.0e-05: 22 Number of HSP's gapped: 1835 Number of HSP's successfully gapped: 22 Length of query: 367 Length of database: 38,378,431 Length adjustment: 108 Effective length of query: 259 Effective length of database: 25,922,359 Effective search space: 6713890981 Effective search space used: 6713890981 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 116 (49.3 bits)