BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0303.0013.p (376 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value lin0980 [GEPR] COG0477 Permeases of the major facilitator superf... 246 4e-65 PA0246 [GEPR] COG0477 Permeases of the major facilitator superfa... 196 3e-50 DR0258 [GEPR] COG0477 Permeases of the major facilitator superfa... 182 6e-46 BS_mdr [GEPR] COG0477 Permeases of the major facilitator superfa... 169 5e-42 VC1597 [GEPR] COG0477 Permeases of the major facilitator superfa... 158 1e-38 >lin0980 [GEPR] COG0477 Permeases of the major facilitator superfamily Length = 491 Score = 246 bits (628), Expect = 4e-65, Method: Compositional matrix adjust. Identities = 128/305 (41%), Positives = 181/305 (59%) Query: 3 GVLLATFLAAIEGTVTGPAGPAIVGDFQGMQWLSWIFTAYLLAMAVTTPIFGKLSDLMGR 62 V +ATF+AAIEGT+ A P IV G++ ++WIF+ YLL AVT PI+GKLSDL GR Sbjct: 12 AVFVATFMAAIEGTIVSTAMPTIVSQLDGIELMNWIFSVYLLTSAVTVPIYGKLSDLYGR 71 Query: 63 KPVFIGGVAVFLLGSLLCGVAQSMEQLIVFRGLQGIGAGALIPMTFTIIGDIYSIQERAK 122 K +F+ +F++GS LCG AQ+MEQLI+FR +QGIGAG ++P T TII D+Y ++RAK Sbjct: 72 KNIFVIATIIFIIGSSLCGFAQNMEQLIIFRAIQGIGAGGILPSTMTIIADVYPFEKRAK 131 Query: 123 TQGLLSSVWGISSLVGPLLGGYVVDYLSWRWVFVFNLPFGLLSIVFISRFLKEENVRRKA 182 G + S WGI+ + GPL+GG++VD LSW W+F N+P GLL+I+ I +L+EE R K Sbjct: 132 VLGFMGSAWGIAGVFGPLVGGFLVDQLSWHWIFFINVPIGLLTILLILLYLREEVERVKL 191 Query: 183 KLDLPGVXXXXXXXXXXXXXXXXXXQNLPWTSPXXXXXXXXXXXXXXIFWRVERRASEPM 242 +D G ++L WT P F+ VE+RA +P+ Sbjct: 192 PIDYLGASLFTVALLGLLFALQRAGESLNWTEPLVVILFAVSILLFVAFYFVEKRAKDPI 251 Query: 243 LPLGLFSIRNITVSTGANLLVSTLIIGLTTYVPLWVQGVXXXXXXXXXXXXXPMSVGWVL 302 +P LF + + L+S +IG+ Y+P+W QG+ P+SV W++ Sbjct: 252 MPFVLFKNPVVLIGNLIGFLISAFLIGINVYIPMWAQGMLGHGATIAGFMLAPLSVTWII 311 Query: 303 GSFAG 307 GSF G Sbjct: 312 GSFIG 316 >PA0246 [GEPR] COG0477 Permeases of the major facilitator superfamily Length = 501 Score = 196 bits (499), Expect = 3e-50, Method: Compositional matrix adjust. Identities = 115/306 (37%), Positives = 168/306 (54%), Gaps = 1/306 (0%) Query: 1 LTGVLLATFLAAIEGTVTGPAGPAIVGDFQGMQWLSWIFTAYLLAMAVTTPIFGKLSDLM 60 L G++L+ FL+A++ T+ + PAI D+ + L+W+ +AY++AM V+ PI+GKL DL Sbjct: 15 LAGLMLSIFLSALDQTIVAVSLPAISADYADLDLLAWVISAYMVAMTVSMPIYGKLGDLY 74 Query: 61 GRKPVFIGGVAVFLLGSLLCGVAQSMEQLIVFRGLQGIGAGALIPMTFTIIGDIYSIQER 120 GR+ + + + VF SLLCG+AQSM QL++ R LQGIGAG L+ ++ IIGDI +ER Sbjct: 75 GRRRLMLFAIVVFTAASLLCGLAQSMGQLVLARVLQGIGAGGLMAVSQAIIGDIVPPRER 134 Query: 121 AKTQGLLSSVWGISSLVGPLLGGYVVDYLSWRWVFVFNLPFGLLSIVFISRFLKEENV-R 179 + QG SS++ I+S+ GP+LGG + +YLSW WVF NLP GLL++ R L V R Sbjct: 135 GRYQGYFSSMYAIASVAGPVLGGLLTEYLSWHWVFWINLPIGLLALAISRRTLVGLPVPR 194 Query: 180 RKAKLDLPGVXXXXXXXXXXXXXXXXXXQNLPWTSPXXXXXXXXXXXXXXIFWRVERRAS 239 R+ +D G+ Q W+ P +F ERR Sbjct: 195 RQPVIDYLGIVLMVVGLTALLLAITQIGQGAAWSDPALLGLFALAALALLLFVAQERRFR 254 Query: 240 EPMLPLGLFSIRNITVSTGANLLVSTLIIGLTTYVPLWVQGVXXXXXXXXXXXXXPMSVG 299 EP++P+ LF++R T+S S I LT VPL Q V P+ +G Sbjct: 255 EPLVPMQLFAVRAATLSWLVAFFASFQAISLTVLVPLRYQSVTGAGADSAALHLLPLVMG 314 Query: 300 WVLGSF 305 LG++ Sbjct: 315 LPLGAY 320 >DR0258 [GEPR] COG0477 Permeases of the major facilitator superfamily Length = 544 Score = 182 bits (463), Expect = 6e-46, Method: Compositional matrix adjust. Identities = 112/305 (36%), Positives = 151/305 (49%), Gaps = 3/305 (0%) Query: 3 GVLLATFLAAIEGTVTGPAGPAIVGDFQGMQWLSWIFTAYLLAMAVTTPIFGKLSDLMGR 62 G+L FL+++ TV G A P ++ D G +W FTAY L VT PI G LSD GR Sbjct: 26 GILTVLFLSSLNMTVVGSAMPRVISDLGGFHLYAWAFTAYSLTTTVTIPIVGTLSDRYGR 85 Query: 63 KPVFIGGVAVFLLGSLLCGVAQSMEQLIVFRGLQGIGAGALIPMTFTIIGDIYSIQERAK 122 +P+ + G+ VF LGS+ G QSMEQLI R +QGIG G L+ M+FT I D+++ ER + Sbjct: 86 RPLILLGIVVFALGSMGLGFVQSMEQLIALRAIQGIGGGTLMAMSFTAIADLFTPIERGR 145 Query: 123 TQGLLSSVWGISSLVGPLLGGYVVDYLSWRWVFVFNLPFGLLSIVFISRFLK---EENVR 179 QG +VWGISS+VGPL+GG++ D+ WR VF NLPF LL++ FI RF + Sbjct: 146 YQGYTGAVWGISSVVGPLVGGFLTDHFGWRSVFFLNLPFALLALFFIWRFFRLPAPTPGS 205 Query: 180 RKAKLDLPGVXXXXXXXXXXXXXXXXXXQNLPWTSPXXXXXXXXXXXXXXIFWRVERRAS 239 K D G W SP + R Sbjct: 206 VKGGFDALGATLLAGAVTTLTLAVSWGGGTYAWDSPRILGLLGATLALGAWYAAHSARQE 265 Query: 240 EPMLPLGLFSIRNITVSTGANLLVSTLIIGLTTYVPLWVQGVXXXXXXXXXXXXXPMSVG 299 +L LGL R I +++ A LVS + Y+PL++QGV P+ G Sbjct: 266 RAILDLGLLRDRGILLASLAGFLVSAGMYAAILYLPLYMQGVRGSSASGSGLALAPLMGG 325 Query: 300 WVLGS 304 +L S Sbjct: 326 MILTS 330 >BS_mdr [GEPR] COG0477 Permeases of the major facilitator superfamily Length = 426 Score = 169 bits (429), Expect = 5e-42, Method: Compositional matrix adjust. Identities = 101/303 (33%), Positives = 153/303 (50%), Gaps = 9/303 (2%) Query: 10 LAAIEGTVTGPAGPAIVGDFQGMQWLSWIFTAYLLAMAVTTPIFGKLSDLMGRKPVFIGG 69 ++A++ T+ A IV D +W+ +Y++A+ PI+GKLSD+ GRK Sbjct: 24 MSAMDNTIVATAMGNIVADLGSFDKFAWVTASYMVAVMAGMPIYGKLSDMYGRKRFSCLD 83 Query: 70 VAVFLLGSLLCGVAQSMEQLIVFRGLQGIGAGALIPMTFTIIGDIYSIQERAKTQGLLSS 129 + L+GS LCG+AQ+M QLI+FR +QGIG GAL+P+ FTII D++ ++R K G+ + Sbjct: 84 LFFSLIGSALCGIAQTMNQLIIFRAIQGIGGGALLPIAFTIIFDLFPPEKRGKMSGMFGA 143 Query: 130 VWGISSLVGPLLGGYVVDYLSWRWVFVFNLPFGLLSIVFISRFLKEENVRRKAKLDLPGV 189 V+G+SS++GPLLG + D +SW WVF N+P G LS+ FI R+ KE RK K+D G Sbjct: 144 VFGLSSVLGPLLGAIITDSISWHWVFYINVPIGALSLFFIIRYYKESLEHRKQKIDWGGA 203 Query: 190 XXXXXXXXXXXXXXXXXXQNLPWTSPXXXXXXXXXXXXXXIFWRVERRASEPMLPLGLFS 249 + W S F+ VE++A +P +S Sbjct: 204 ITLVVSIVCLMFALELGGKTYDWNSIQIIGLFIVFAVFFIAFFIVEKKAEDP----ARYS 259 Query: 250 IRNITVSTGAN-----LLVSTLIIGLTTYVPLWVQGVXXXXXXXXXXXXXPMSVGWVLGS 304 R+ ++ L + I L ++P +VQ V PM +G V+ S Sbjct: 260 ARSSRMACLRQRQILAFLYEGIFIILAVFIPTFVQPVYASSAASAGFILTPMMIGSVIRS 319 Query: 305 FAG 307 G Sbjct: 320 MIG 322 >VC1597 [GEPR] COG0477 Permeases of the major facilitator superfamily Length = 477 Score = 158 bits (400), Expect = 1e-38, Method: Compositional matrix adjust. Identities = 93/303 (30%), Positives = 161/303 (53%), Gaps = 1/303 (0%) Query: 4 VLLATFLAAIEGTVTGPAGPAIVGDFQGMQWLSWIFTAYLLAMAVTTPIFGKLSDLMGRK 63 V L FLAA++ T+ A PAIV D +Q SWI Y+LA A + P++G L D GRK Sbjct: 17 VFLPMFLAAVDQTLLATATPAIVRDLGNLQLSSWIAIGYMLASAASVPVYGWLGDQYGRK 76 Query: 64 PVFIGGVAVFLLGSLLCGVAQSMEQLIVFRGLQGIGAGALIPMTFTIIGDIYSIQERAKT 123 P+ + +++F LGS++C A +M L+ R +QG+G+G L+ ++ +IG++ +ERA+ Sbjct: 77 PMLMIALSIFALGSVICAFAMNMPWLVAGRIVQGLGSGGLMSLSQALIGELVPPRERARF 136 Query: 124 QGLLSSVWGISSLVGPLLGGYVVDYLSWRWVFVFNLPFGLLSIVFISRFLKEENVRR-KA 182 QG SS++ ++S+ GP+LGG +V YLSW+W+F FNLP +++ ++RF ++ R+ +A Sbjct: 137 QGYFSSLFAVASIGGPVLGGVLVTYLSWQWLFWFNLPLVGIALYRLARFQFVQSSRKSEA 196 Query: 183 KLDLPGVXXXXXXXXXXXXXXXXXXQNLPWTSPXXXXXXXXXXXXXXIFWRVERRASEPM 242 +D+ G+ PW S +F ++R + Sbjct: 197 SIDVAGLILFPSMMTLIIYWLSAGGHYFPWFSVTSISLVSAFVLTSLLFIAQQKRVTASF 256 Query: 243 LPLGLFSIRNITVSTGANLLVSTLIIGLTTYVPLWVQGVXXXXXXXXXXXXXPMSVGWVL 302 LPL L + R I + + + + + L ++P+++Q P+S G + Sbjct: 257 LPLRLLARREIHIPLLSTFIFAACLFALIFFLPIFLQLGLRVNAAQSGLLLIPLSCGIIG 316 Query: 303 GSF 305 G++ Sbjct: 317 GAY 319 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.328 0.145 0.470 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 24,143,700 Number of extensions: 849753 Number of successful extensions: 3086 Number of sequences better than 1.0e-05: 142 Number of HSP's gapped: 3056 Number of HSP's successfully gapped: 145 Length of query: 376 Length of database: 38,378,431 Length adjustment: 108 Effective length of query: 268 Effective length of database: 25,922,359 Effective search space: 6947192212 Effective search space used: 6947192212 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.7 bits) S2: 117 (49.7 bits)