BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0303.0010.p (432 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value PAB0724 [GEPR] COG0477 Permeases of the major facilitator superf... 91 5e-18 CAC0458 [GEPR] COG0477 Permeases of the major facilitator superf... 87 6e-17 AGc1397 [C] COG1622 Heme/copper-type cytochrome/quinol oxidases,... 59 2e-08 AGc1396 [R] COG5342 Invasion protein B, involved in pathogenesis 59 2e-08 AGc1392 [R] COG0312 Predicted Zn-dependent proteases and their i... 59 2e-08 >PAB0724 [GEPR] COG0477 Permeases of the major facilitator superfamily Length = 418 Score = 90.5 bits (223), Expect = 5e-18, Method: Compositional matrix adjust. Identities = 98/384 (25%), Positives = 176/384 (45%), Gaps = 22/384 (5%) Query: 17 NKPFVLLITAQLVSNIGDWLHILALLTMVGLKWQASPWEITAVSLCMAVPMLLGGPFAGY 76 N+ F L + + +S +G + +AL V L S +T L +P ++ P AG Sbjct: 2 NRNFWLFVAGRFISQLGWAIQEVALPLYV-LDVTHSAKIMTLFVLADLIPSIITTPIAGV 60 Query: 77 LSDKVDRKGIMIGSDLVRAGIVACLVFAGSLWQVYVLLLAK---GVMDVLFSPAKSGKLK 133 D+ DRK IM+G DL+R G++ LV A + + LL+ + +M+ FS Sbjct: 61 FGDRYDRKKIMVGFDLIR-GVLLFLVIAFNFLTIRCLLVVQVIMAIMNAFFSAGTGAMYP 119 Query: 134 ELVPPGQMDQAVALSSSIEQITKIIGPALGGLLVAAVGISACYFIDSVSYIASAVILLGL 193 +LV P ++++A ++ S I++++GPA+GG + GI ++++S+ S + + + Sbjct: 120 DLVKPEELERANSIVMSASIISRLVGPAIGGFVYGFGGIKLAILMNAISFFGSGLFEIFI 179 Query: 194 PRVGMFKTGRPNEDGDTARPSFRKEAGAGLRLIGSMPVV--LSGVVMIVAALLVLQIADS 251 ++ N +G + F ++ GL+ I S + L G+V+++ AL A Sbjct: 180 K----YEWKTRNIEGFS---DFVRDIKEGLKFIMSNSFISTLIGLVILLNALGA-PFASI 231 Query: 252 QTVTLFREIPGVNADLLGWCVAASGFGTLLSALAVGRL---GSGKHPL-VFMGTGTLLMG 307 REI ++ G +A G L+ L VG +G++ +F+ G + + Sbjct: 232 IYPYAMREILKFSSQQFGLVESAFMLGALIGNLIVGAFLGAKAGRYIFRLFLVNGAVELI 291 Query: 308 SVFAGAALVTVTGQAGFWMDTMLFGSFVLAGVGAGFVIIPFNSMLQRRTPEAYTGRVFGI 367 + + + + + F + +L + +AG + +P S LQR P GRVF Sbjct: 292 FILIISPFLALPRNSAFMLLLVLGATMGMAG---AMINVPLASKLQRAVPSEIRGRVFST 348 Query: 368 VGSLTSAAVILGPVAGGALVTASG 391 + L A L V G LV G Sbjct: 349 MEMLARIANPLSLVVVGPLVDTIG 372 >CAC0458 [GEPR] COG0477 Permeases of the major facilitator superfamily Length = 409 Score = 86.7 bits (213), Expect = 6e-17, Method: Compositional matrix adjust. Identities = 103/405 (25%), Positives = 186/405 (45%), Gaps = 48/405 (11%) Query: 5 QENVTTAVKLTSNKPFVLLITAQLVSNIGDWLHILALLTMVGLKWQASPWEITAVSLCMA 64 +N T++ ++ F I Q +S +G WL A + ++ SP + ++S+ Sbjct: 5 NKNTFTSLH---HRDFKCFIIGQSISLMGTWLQRTAEVWLL-YTLTKSPLLLGSLSVFQF 60 Query: 65 VPMLLGGPFAGYLSDKVDRKGIMIGSD---LVRAGIVACLVFAGSLWQVYVLLLAK--GV 119 P L+ F+G + D+ +K ++ + +++A + LV+ + + + +LA G+ Sbjct: 61 GPTLIFSVFSGAIVDRFPKKKLLYLTQTVGMLQALALFILVYTNHITAILIFILATIGGL 120 Query: 120 MDVLFSPAKSGKLKELVPPGQMDQAVALSSSIEQITKIIGPALGGLLVAAVGISACYFID 179 L P + ELV + AV+L+SSI + KIIGP++ G+++ G+S C+FI+ Sbjct: 121 STTLDMPTRQSYFMELVGKKDLPNAVSLNSSIFNLAKIIGPSIAGIIMGKFGMSVCFFIN 180 Query: 180 SVSYIASAVILLGLPRVGMFKTGRPNEDGDTARPSFRKEAGAGLRLIGSMPVVLSGVVMI 239 +SY +AV+ G+F + + + + + KE GL+ I ++L Sbjct: 181 FLSY--AAVL------TGLFFIKQKSYAANISSDNIFKEIKEGLKYIYENKILLK----- 227 Query: 240 VAALLVLQIADSQTVTLFREIPGVNADLLGWCVAASGFGTLLSALAVGRL-------GSG 292 AL+ + I + + + +P + +L + G+ LLS++ VG L G Sbjct: 228 --ALIFMAIICTFAMNVDVIVPVFSKTVLHK--GSHGYTFLLSSMGVGSLFGTMKMAGLR 283 Query: 293 KHPLVFMGTGTLLMGSVFAGAALVTVTGQAGFWMDTMLFGSFVLAGVGAGFVIIPF---- 348 + L F LL F+ A V +G L+ + VL +G+GF + F Sbjct: 284 RKNLNF----KLLKIVGFSSAIFQIVAAFSG-----NLYFTAVLV-LGSGFSNLCFLNGS 333 Query: 349 NSMLQRRTPEAYTGRVFGIVGSLTSAAVILGPV-AGGALVTASGP 392 NS+LQ T + Y GRV I L + +G + G A+ GP Sbjct: 334 NSILQLNTSDKYRGRVMSIYTLLNAGTTPIGNLFVGAAMNNLGGP 378 >AGc1397 [C] COG1622 Heme/copper-type cytochrome/quinol oxidases, subunit 2 Length = 542 Score = 58.5 bits (140), Expect = 2e-08, Method: Compositional matrix adjust. Identities = 106/406 (26%), Positives = 172/406 (42%), Gaps = 46/406 (11%) Query: 1 MKENQENVTTAVKLTSNKPFVLLITAQLVSNIGDWLHILALLTMVGLKW-----QASPWE 55 M + + + LT + ++ A L SN G L+ VG W +S Sbjct: 1 MPDRKSPLAPLRHLTYRRIWI----ASLASNFG------GLIQAVGAAWMMTSLSSSENM 50 Query: 56 ITAVSLCMAVPMLLGGPFAGYLSDKVDRKGIMIGSDLVR-AGIVACLVFAGSLWQVYVLL 114 I V ++P+++ +G L+D DR+ IMI + L+ V VFA S W LL Sbjct: 51 IALVQASTSLPIMMFSLISGALADSFDRRRIMISAQLLMLTASVLLTVFAWSGWLTPWLL 110 Query: 115 LAKGVM----DVLFSPAKSGKLKELVPPGQMDQAVALSSSIEQITKIIGPALGGLLVAAV 170 L M L +P+ + E+VP + AV L+S IT+ +GPA+GG++VAA Sbjct: 111 LFFTFMIGCGTALNNPSWQASVGEMVPREDLPAAVTLNSVGFNITRSVGPAIGGIIVAAA 170 Query: 171 GISACYFIDSVSYIASAVILLGLPRVGMFKTGRPNEDGDTARPSFRKEAGAGLRLIGSMP 230 G +A +F++++SY +F+ P R AG+R + P Sbjct: 171 GAAAAFFVNALSYFTLI--------YALFRWQPPKYASTLPREQLLAAISAGMRYVAMSP 222 Query: 231 VVLSGVVMIVAALLVLQIADSQTVTLFREIPGVNADLLGWCVAASGFGTLLSALAVGRLG 290 + G V++ L L + + +P V DL+ +G +L A VG +G Sbjct: 223 NI--GKVLVRGFLFGLSAS-----AILALMPIVARDLVE--GGPLTYGVMLGAFGVGAIG 273 Query: 291 SG--KHPLVFMGTGTLLMGSVFAGAALVT-VTGQAGFWMDTMLFGSFVLAGVGAGFVII- 346 L + + ++ F G A + VT + + + S VLA GA +V+ Sbjct: 274 GALVSARLREVMSSEWIVRVAFLGFAFSSGVTAVS----TSSIITSLVLAIAGACWVLAL 329 Query: 347 -PFNSMLQRRTPEAYTGRVFGIVGSLTSAAVILGPVAGGALVTASG 391 FN+++Q TP GR + +LT + +G G L G Sbjct: 330 SLFNTIVQLSTPRWVVGRALSLYQTLTFGGIAVGSWLWGELAEDYG 375 >AGc1396 [R] COG5342 Invasion protein B, involved in pathogenesis Length = 542 Score = 58.5 bits (140), Expect = 2e-08, Method: Compositional matrix adjust. Identities = 106/406 (26%), Positives = 172/406 (42%), Gaps = 46/406 (11%) Query: 1 MKENQENVTTAVKLTSNKPFVLLITAQLVSNIGDWLHILALLTMVGLKW-----QASPWE 55 M + + + LT + ++ A L SN G L+ VG W +S Sbjct: 1 MPDRKSPLAPLRHLTYRRIWI----ASLASNFG------GLIQAVGAAWMMTSLSSSENM 50 Query: 56 ITAVSLCMAVPMLLGGPFAGYLSDKVDRKGIMIGSDLVR-AGIVACLVFAGSLWQVYVLL 114 I V ++P+++ +G L+D DR+ IMI + L+ V VFA S W LL Sbjct: 51 IALVQASTSLPIMMFSLISGALADSFDRRRIMISAQLLMLTASVLLTVFAWSGWLTPWLL 110 Query: 115 LAKGVM----DVLFSPAKSGKLKELVPPGQMDQAVALSSSIEQITKIIGPALGGLLVAAV 170 L M L +P+ + E+VP + AV L+S IT+ +GPA+GG++VAA Sbjct: 111 LFFTFMIGCGTALNNPSWQASVGEMVPREDLPAAVTLNSVGFNITRSVGPAIGGIIVAAA 170 Query: 171 GISACYFIDSVSYIASAVILLGLPRVGMFKTGRPNEDGDTARPSFRKEAGAGLRLIGSMP 230 G +A +F++++SY +F+ P R AG+R + P Sbjct: 171 GAAAAFFVNALSYFTLI--------YALFRWQPPKYASTLPREQLLAAISAGMRYVAMSP 222 Query: 231 VVLSGVVMIVAALLVLQIADSQTVTLFREIPGVNADLLGWCVAASGFGTLLSALAVGRLG 290 + G V++ L L + + +P V DL+ +G +L A VG +G Sbjct: 223 NI--GKVLVRGFLFGLSAS-----AILALMPIVARDLVE--GGPLTYGVMLGAFGVGAIG 273 Query: 291 SG--KHPLVFMGTGTLLMGSVFAGAALVT-VTGQAGFWMDTMLFGSFVLAGVGAGFVII- 346 L + + ++ F G A + VT + + + S VLA GA +V+ Sbjct: 274 GALVSARLREVMSSEWIVRVAFLGFAFSSGVTAVS----TSSIITSLVLAIAGACWVLAL 329 Query: 347 -PFNSMLQRRTPEAYTGRVFGIVGSLTSAAVILGPVAGGALVTASG 391 FN+++Q TP GR + +LT + +G G L G Sbjct: 330 SLFNTIVQLSTPRWVVGRALSLYQTLTFGGIAVGSWLWGELAEDYG 375 >AGc1392 [R] COG0312 Predicted Zn-dependent proteases and their inactivated homologs Length = 542 Score = 58.5 bits (140), Expect = 2e-08, Method: Compositional matrix adjust. Identities = 106/406 (26%), Positives = 172/406 (42%), Gaps = 46/406 (11%) Query: 1 MKENQENVTTAVKLTSNKPFVLLITAQLVSNIGDWLHILALLTMVGLKW-----QASPWE 55 M + + + LT + ++ A L SN G L+ VG W +S Sbjct: 1 MPDRKSPLAPLRHLTYRRIWI----ASLASNFG------GLIQAVGAAWMMTSLSSSENM 50 Query: 56 ITAVSLCMAVPMLLGGPFAGYLSDKVDRKGIMIGSDLVR-AGIVACLVFAGSLWQVYVLL 114 I V ++P+++ +G L+D DR+ IMI + L+ V VFA S W LL Sbjct: 51 IALVQASTSLPIMMFSLISGALADSFDRRRIMISAQLLMLTASVLLTVFAWSGWLTPWLL 110 Query: 115 LAKGVM----DVLFSPAKSGKLKELVPPGQMDQAVALSSSIEQITKIIGPALGGLLVAAV 170 L M L +P+ + E+VP + AV L+S IT+ +GPA+GG++VAA Sbjct: 111 LFFTFMIGCGTALNNPSWQASVGEMVPREDLPAAVTLNSVGFNITRSVGPAIGGIIVAAA 170 Query: 171 GISACYFIDSVSYIASAVILLGLPRVGMFKTGRPNEDGDTARPSFRKEAGAGLRLIGSMP 230 G +A +F++++SY +F+ P R AG+R + P Sbjct: 171 GAAAAFFVNALSYFTLI--------YALFRWQPPKYASTLPREQLLAAISAGMRYVAMSP 222 Query: 231 VVLSGVVMIVAALLVLQIADSQTVTLFREIPGVNADLLGWCVAASGFGTLLSALAVGRLG 290 + G V++ L L + + +P V DL+ +G +L A VG +G Sbjct: 223 NI--GKVLVRGFLFGLSAS-----AILALMPIVARDLVE--GGPLTYGVMLGAFGVGAIG 273 Query: 291 SG--KHPLVFMGTGTLLMGSVFAGAALVT-VTGQAGFWMDTMLFGSFVLAGVGAGFVII- 346 L + + ++ F G A + VT + + + S VLA GA +V+ Sbjct: 274 GALVSARLREVMSSEWIVRVAFLGFAFSSGVTAVS----TSSIITSLVLAIAGACWVLAL 329 Query: 347 -PFNSMLQRRTPEAYTGRVFGIVGSLTSAAVILGPVAGGALVTASG 391 FN+++Q TP GR + +LT + +G G L G Sbjct: 330 SLFNTIVQLSTPRWVVGRALSLYQTLTFGGIAVGSWLWGELAEDYG 375 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.322 0.138 0.402 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 29,978,423 Number of extensions: 1296002 Number of successful extensions: 3810 Number of sequences better than 1.0e-05: 46 Number of HSP's gapped: 3764 Number of HSP's successfully gapped: 46 Length of query: 432 Length of database: 38,378,431 Length adjustment: 109 Effective length of query: 323 Effective length of database: 25,807,025 Effective search space: 8335669075 Effective search space used: 8335669075 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 117 (49.7 bits)