BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0303.0009.p (261 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value BS_ecsA [V] COG1131 ABC-type multidrug transport system, ATPase ... 168 7e-42 L58125 [V] COG1131 ABC-type multidrug transport system, ATPase c... 155 6e-38 SP0522 [V] COG1131 ABC-type multidrug transport system, ATPase c... 151 1e-36 SA1655 [V] COG1131 ABC-type multidrug transport system, ATPase c... 145 9e-35 SPy1729 [V] COG1131 ABC-type multidrug transport system, ATPase ... 138 7e-33 >BS_ecsA [V] COG1131 ABC-type multidrug transport system, ATPase component Length = 247 Score = 168 bits (426), Expect = 7e-42, Method: Compositional matrix adjust. Identities = 90/222 (40%), Positives = 121/222 (54%), Gaps = 9/222 (4%) Query: 49 ISNISFQVXXXXXXXXXXPNGAGKSTTIKTLLGLLKHAKARVKLGGA---------NKSY 99 + N+SF + NGAGKSTTI+ ++GL+ K ++L G + Sbjct: 19 LKNVSFTLEPNQIVGLIGLNGAGKSTTIRHIIGLMDPHKGSIELNGKTFAEDPEGYRSQF 78 Query: 100 AYVPEQPVFYEDLTLWEHLDLAAAAYGLSYETFGATAEKLLSQFGMEQVRNDLPAGFSXX 159 Y+PE PV YE+LTL EHL+L A AYGLS ET LL +F ME+ PA FS Sbjct: 79 TYIPETPVLYEELTLMEHLELTAMAYGLSKETMEKRLPPLLKEFRMEKRLKWFPAHFSKG 138 Query: 160 XXXXXXXXXXFLVQPDIYIVDEPFIGLDPRATKDFXXXXXXXXXXXXXVLMSTHVLDTAE 219 FL +P +YI+DEPF+GLDP A VLMSTH+L TAE Sbjct: 139 MKQKVMIMCAFLAEPALYIIDEPFLGLDPLAINALLERMNEAKKGGASVLMSTHILATAE 198 Query: 220 KICDSFVLISGGRMTATGTLEDIRAQAGLPEASLFDCFDELT 261 + CDSF+++ G + A GTL ++R Q G+ +A+L D + ELT Sbjct: 199 RYCDSFIILHNGEVRARGTLSELREQFGMKDAALDDLYLELT 240 >L58125 [V] COG1131 ABC-type multidrug transport system, ATPase component Length = 256 Score = 155 bits (392), Expect = 6e-38, Method: Compositional matrix adjust. Identities = 85/231 (36%), Positives = 125/231 (54%), Gaps = 10/231 (4%) Query: 40 AGYEAGDRRISNISFQVXXXXXXXXXXPNGAGKSTTIKTLLGLLKHAKARVKLGGA---- 95 GY G + +++F++ NGAGKSTTI+ ++GLL +++L G Sbjct: 9 GGY-LGHPVLKDLNFEIKEGELVGLIGLNGAGKSTTIQEIIGLLTPYSGQIELDGLTLQQ 67 Query: 96 -----NKSYAYVPEQPVFYEDLTLWEHLDLAAAAYGLSYETFGATAEKLLSQFGMEQVRN 150 + ++PE P YE+LTL EH+++ A AYG+S + AEKLL F + + Sbjct: 68 DLEGYRRKIGFIPETPSLYEELTLREHIEVTALAYGISVDLAFERAEKLLETFRLSDKLD 127 Query: 151 DLPAGFSXXXXXXXXXXXXFLVQPDIYIVDEPFIGLDPRATKDFXXXXXXXXXXXXXVLM 210 P FS FLV+P +YIVDEPF+GLDP A +D +LM Sbjct: 128 WFPVNFSKGMKQKVMIICAFLVEPSLYIVDEPFMGLDPLAIQDLIDLMIDMKKSGSSILM 187 Query: 211 STHVLDTAEKICDSFVLISGGRMTATGTLEDIRAQAGLPEASLFDCFDELT 261 STH+L TAEK CD F+++ G++ A GT+E++R Q P+ASL + + LT Sbjct: 188 STHILSTAEKFCDKFIVLHEGQVVAFGTVEELRTQFNQPKASLDEIYLALT 238 >SP0522 [V] COG1131 ABC-type multidrug transport system, ATPase component Length = 243 Score = 151 bits (381), Expect = 1e-36, Method: Compositional matrix adjust. Identities = 83/222 (37%), Positives = 113/222 (50%), Gaps = 9/222 (4%) Query: 49 ISNISFQVXXXXXXXXXXPNGAGKSTTIKTLLGLLKHAKARVKLGGA---------NKSY 99 + ++SF V NGAGKSTTI ++GLL + + G K Sbjct: 17 LKDVSFTVESGQLVGLIGLNGAGKSTTINEIIGLLAPYSGSININGLTLQGDATSYRKQI 76 Query: 100 AYVPEQPVFYEDLTLWEHLDLAAAAYGLSYETFGATAEKLLSQFGMEQVRNDLPAGFSXX 159 Y+PE P YE+LTL EH++ A AYG+ + E LL F +EQ + P FS Sbjct: 77 GYIPETPSLYEELTLREHIETVAMAYGIEQKVAFERVEPLLKMFRLEQKLDWFPVHFSKG 136 Query: 160 XXXXXXXXXXFLVQPDIYIVDEPFIGLDPRATKDFXXXXXXXXXXXXXVLMSTHVLDTAE 219 F+V P ++IVDEPF+GLDP A D +LMSTHVLD+AE Sbjct: 137 MKQKVMIICAFVVDPSLFIVDEPFLGLDPLAISDLIQLLEVEKQKGKSILMSTHVLDSAE 196 Query: 220 KICDSFVLISGGRMTATGTLEDIRAQAGLPEASLFDCFDELT 261 K+CD+FV++ G + + G L +R +PEASL D + LT Sbjct: 197 KMCDAFVILHKGEVRSKGNLLQLREAFDMPEASLNDIYLALT 238 >SA1655 [V] COG1131 ABC-type multidrug transport system, ATPase component Length = 246 Score = 145 bits (365), Expect = 9e-35, Method: Compositional matrix adjust. Identities = 79/222 (35%), Positives = 114/222 (51%), Gaps = 9/222 (4%) Query: 49 ISNISFQVXXXXXXXXXXPNGAGKSTTIKTLLGLLKHAKARVKLGGAN---------KSY 99 I +I+F++ NGAGKSTTIK +LGLL + + + N + Sbjct: 18 IKDINFELNKGEIVGLIGLNGAGKSTTIKHMLGLLTPMEGSLSISDININDDIEAYRRKL 77 Query: 100 AYVPEQPVFYEDLTLWEHLDLAAAAYGLSYETFGATAEKLLSQFGMEQVRNDLPAGFSXX 159 +Y+PE PV YE+LTL EH+++ A AY + + A LL F +E P+ FS Sbjct: 78 SYIPESPVIYEELTLEEHIEMTAMAYDIDRDEAMNRAMPLLKTFRLENELKVFPSHFSKG 137 Query: 160 XXXXXXXXXXFLVQPDIYIVDEPFIGLDPRATKDFXXXXXXXXXXXXXVLMSTHVLDTAE 219 F+V P++YI+DEPF+GLDP + VLMSTH+L TAE Sbjct: 138 MKQKVMIICAFIVNPELYIIDEPFLGLDPLGIQSMLDLMVEKKNEGRTVLMSTHILATAE 197 Query: 220 KICDSFVLISGGRMTATGTLEDIRAQAGLPEASLFDCFDELT 261 + CD F+++ G + A G LE +R Q GL +L D + +T Sbjct: 198 RYCDRFIILDEGEVVAFGDLEALRQQTGLHNQTLDDIYIHVT 239 >SPy1729 [V] COG1131 ABC-type multidrug transport system, ATPase component Length = 241 Score = 138 bits (348), Expect = 7e-33, Method: Compositional matrix adjust. Identities = 79/222 (35%), Positives = 113/222 (50%), Gaps = 9/222 (4%) Query: 49 ISNISFQVXXXXXXXXXXPNGAGKSTTIKTLLGLLKHAKARVKLGG---ANKSYAY---- 101 ++++SF V NGAGKSTTI ++G LK + + + G A + AY Sbjct: 17 LNDVSFSVDNGELVGLIGLNGAGKSTTINEIIGFLKPYQGSISIDGLTLAENAVAYRQKI 76 Query: 102 --VPEQPVFYEDLTLWEHLDLAAAAYGLSYETFGATAEKLLSQFGMEQVRNDLPAGFSXX 159 +PE P YE+LTL EH++ A AY + E A+ L F + P FS Sbjct: 77 GFIPETPSLYEELTLSEHINTVAMAYDIDLEVAQKRAQPFLEMFRLTDKLEWFPVNFSKG 136 Query: 160 XXXXXXXXXXFLVQPDIYIVDEPFIGLDPRATKDFXXXXXXXXXXXXXVLMSTHVLDTAE 219 F++ P ++I+DEPF+GLDP A D +LMSTHVLD+AE Sbjct: 137 MKQKVMIICAFVIDPSLFILDEPFLGLDPLAISDLIQTLEVEKAKGKSILMSTHVLDSAE 196 Query: 220 KICDSFVLISGGRMTATGTLEDIRAQAGLPEASLFDCFDELT 261 ++CD FV++ G++ A GTL D++ G ASL D + LT Sbjct: 197 RMCDRFVILHHGQVRAQGTLADLQEAFGDRSASLNDIYLALT 238 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.316 0.134 0.383 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 16,778,317 Number of extensions: 576043 Number of successful extensions: 4756 Number of sequences better than 1.0e-05: 334 Number of HSP's gapped: 4711 Number of HSP's successfully gapped: 349 Length of query: 261 Length of database: 38,378,431 Length adjustment: 104 Effective length of query: 157 Effective length of database: 26,383,695 Effective search space: 4142240115 Effective search space used: 4142240115 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 115 (48.9 bits)