BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0303.0006.p (582 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value BS_kinB [T] COG0642 Signal transduction histidine kinase 158 2e-38 BS_kinA_3 [T] COG0642 Signal transduction histidine kinase 155 2e-37 BS_kinC_2 [T] COG0642 Signal transduction histidine kinase 144 4e-34 atoS_3 [T] COG0642 Signal transduction histidine kinase 132 1e-30 TM1359_4 [T] COG0642 Signal transduction histidine kinase 117 4e-26 >BS_kinB [T] COG0642 Signal transduction histidine kinase Length = 429 Score = 158 bits (400), Expect = 2e-38, Method: Compositional matrix adjust. Identities = 85/241 (35%), Positives = 139/241 (57%), Gaps = 11/241 (4%) Query: 327 VLIVQDLTEEEKFRSELDNVERLTLVGQMAAGITHEIRNPMAVVRGFLQLM------REK 380 + I++ + E RS+L + E++T+V ++AA + HE+RNP+ VVRGF+QL+ + K Sbjct: 188 IYIIESIAENIALRSQLIHSEKMTIVSELAASVAHEVRNPLTVVRGFVQLLFNDETLQNK 247 Query: 381 SPGEMDSYYQIVIEELDRANGIINDFLSLAQSRISDKEPVQLHHIIKELAPLLWADANLR 440 S + Y ++V+ ELDRA GII ++L +A+ ++ +KE L +IKE + L+ + AN + Sbjct: 248 SSAD---YKKLVLSELDRAQGIITNYLDMAKQQLYEKEVFDLSALIKETSSLMVSYANYK 304 Query: 441 GQSVEXXXXXXXXXXXXXXREIKQLILNLARNGMEAM-EPKGELLLETHFNENKVELIIQ 499 +VE + +KQ ++NL +N +EA+ KG + + N + + + I Sbjct: 305 SVTVEAETEPDLLIYGDATK-LKQAVINLMKNSIEAVPHGKGMIHISAKRNGHTIMINIT 363 Query: 500 DTGSGIPQSQLEKLFVPFYTTKNQGTGLGLPLCLSIAERHHGSIRVESQVGKGTAFIVSF 559 D G G+ Q++KL P+Y+ K GTGLGL + SI E HHG+I S KGT + Sbjct: 364 DNGVGMTDHQMQKLGEPYYSLKTNGTGLGLTVTFSIIEHHHGTISFNSSFQKGTTVTIKL 423 Query: 560 P 560 P Sbjct: 424 P 424 >BS_kinA_3 [T] COG0642 Signal transduction histidine kinase Length = 236 Score = 155 bits (392), Expect = 2e-37, Method: Compositional matrix adjust. Identities = 87/234 (37%), Positives = 138/234 (58%), Gaps = 2/234 (0%) Query: 328 LIVQDLTEEEKFRSELDNVERLTLVGQMAAGITHEIRNPMAVVRGFLQLMREKSPGEMDS 387 +I++D++E ++ + E+L++ GQ+AAGI HEIRNP+ ++GFLQLM+ G + Sbjct: 2 VILRDISERKQTEELMLKSEKLSIAGQLAAGIAHEIRNPLTAIKGFLQLMKPTMEGN-EH 60 Query: 388 YYQIVIEELDRANGIINDFLSLAQSRISD-KEPVQLHHIIKELAPLLWADANLRGQSVEX 446 Y+ IV EL R I+++ L LA+ + + KE + L +I E++ LL ANL G + Sbjct: 61 YFDIVFSELSRIELILSELLMLAKPQQNAVKEYLNLKKLIGEVSALLETQANLNGIFIRT 120 Query: 447 XXXXXXXXXXXXXREIKQLILNLARNGMEAMEPKGELLLETHFNENKVELIIQDTGSGIP 506 ++KQ+ +NL +N +E+M G + + +E+ V + ++D G GIP Sbjct: 121 SYEKDSIYINGDQNQLKQVFINLIKNAVESMPDGGTVDIIITEDEHSVHVTVKDEGEGIP 180 Query: 507 QSQLEKLFVPFYTTKNQGTGLGLPLCLSIAERHHGSIRVESQVGKGTAFIVSFP 560 + L ++ PF TTK +GTGLGL + +I E H G I V+S KGTAF +SFP Sbjct: 181 EKVLNRIGEPFLTTKEKGTGLGLMVTFNIIENHQGVIHVDSHPEKGTAFKISFP 234 >BS_kinC_2 [T] COG0642 Signal transduction histidine kinase Length = 248 Score = 144 bits (363), Expect = 4e-34, Method: Compositional matrix adjust. Identities = 80/238 (33%), Positives = 140/238 (58%), Gaps = 10/238 (4%) Query: 332 DLTEEEKFRSELDNV--------ERLTLVGQMAAGITHEIRNPMAVVRGFLQLMREKSPG 383 D+TE ++ EL + ++L++ ++AAGI HE+RNP+ V GFLQ+M+ + P Sbjct: 7 DITERKEAEDELIEINERLARESQKLSITSELAAGIAHEVRNPLTSVSGFLQIMKTQYPD 66 Query: 384 EMDSYYQIVIEELDRANGIINDFLSLAQSRISDKEPVQLHHIIKELAPLLWADANLRGQS 443 D Y+ I+ E+ R + ++++ L LA+ + + QL+ I+K++ LL +A L Sbjct: 67 RKD-YFDIIFSEIKRIDLVLSELLLLAKPQAITFKTHQLNEILKQVTTLLDTNAILSNIV 125 Query: 444 VEXX-XXXXXXXXXXXXREIKQLILNLARNGMEAMEPKGELLLETHFNENKVELIIQDTG 502 +E ++KQ+ +N+ +NG+EAM G + + T + + ++D G Sbjct: 126 IEKNFKETDGCMINGDENQLKQVFINIIKNGIEAMPKGGVVTISTAKTASHAVISVKDEG 185 Query: 503 SGIPQSQLEKLFVPFYTTKNQGTGLGLPLCLSIAERHHGSIRVESQVGKGTAFIVSFP 560 +G+PQ +L+++ PFY+TK +GTGLGLP+CL I + H G +++ES+ GKG+ F V P Sbjct: 186 NGMPQEKLKQIGKPFYSTKEKGTGLGLPICLRILKEHDGELKIESEAGKGSVFQVVLP 243 >atoS_3 [T] COG0642 Signal transduction histidine kinase Length = 278 Score = 132 bits (333), Expect = 1e-30, Method: Compositional matrix adjust. Identities = 76/244 (31%), Positives = 137/244 (56%), Gaps = 4/244 (1%) Query: 327 VLIVQDLTEEEKFRSELDNVERLTLVGQMAAGITHEIRNPMAVVRGFLQLMREKSPGEM- 385 ++I DLT ++ + + ERL +G++ AG+ HE+RNP+ +RG++Q++R+++ + Sbjct: 34 LVIFSDLTARKETQRRMAQAERLATLGELMAGVAHEVRNPLTAIRGYVQILRQQTSDPIH 93 Query: 386 DSYYQIVIEELDRANGIINDFLSLAQSRISDKEPVQLHHIIKELAPLLWADANLRGQSVE 445 Y +V++E+D N +I L ++ R S + V L+ +++E L+ A ++ + Sbjct: 94 QEYLSVVLKEIDSINKVIQQLLEFSRPRHSQWQQVSLNALVEETLVLVQT-AGVQARVDF 152 Query: 446 XXXXXXXXXXXXXXREI-KQLILNLARNGMEAMEPKGELLLET-HFNENKVELIIQDTGS 503 RE+ KQ++LN+ N ++A+ +G++ ++T +++++ + I+D G Sbjct: 153 ISELDNELSPINADRELLKQVLLNILINAVQAISARGKIRIQTWQYSDSQQAISIEDNGC 212 Query: 504 GIPQSQLEKLFVPFYTTKNQGTGLGLPLCLSIAERHHGSIRVESQVGKGTAFIVSFPYVQ 563 GI S +K+F PF+TTK GTGLGL L I H G IRV S G G F + P Sbjct: 213 GIDLSLQKKIFDPFFTTKASGTGLGLALSQRIINAHQGDIRVASLPGYGATFTLILPINP 272 Query: 564 EGRQ 567 +G Q Sbjct: 273 QGNQ 276 >TM1359_4 [T] COG0642 Signal transduction histidine kinase Length = 275 Score = 117 bits (294), Expect = 4e-26, Method: Compositional matrix adjust. Identities = 77/270 (28%), Positives = 132/270 (48%), Gaps = 7/270 (2%) Query: 302 QYDSRTYNVFSAPXXXXXXXXXXXRVLIVQDLTEEEKFRSELDNVERLTLVGQMAAGITH 361 ++ R +N+ +P ++ + D+TE K+ E ERL+++G+M A + H Sbjct: 8 KFGERYFNIRFSPFRNAKTQLLEGVIITIDDVTELYKYEEERKRRERLSILGEMTARVAH 67 Query: 362 EIRNPMAVVRGFLQLMRE--KSPGEMDSYYQIVIEELDRANGIINDFLSLAQSR-ISDKE 418 EIRNP+ ++ GF+ M++ P + Y I+ EL R I+ + L ++ R + + Sbjct: 68 EIRNPITIIGGFIMRMKKHLDDPETLKKYINIITNELSRLETIVKEILEYSKERQVLEFT 127 Query: 419 PVQLHHIIKELAPLLWADANLRGQSVEX--XXXXXXXXXXXXXREIKQLILNLARNGMEA 476 L+ +I+E+ L + +R +++ IKQ+++NL +N +EA Sbjct: 128 EFNLNELIREVYVLF--EEKIRKMNIDFCFETDNEDLRVEADRTRIKQVLINLVQNAIEA 185 Query: 477 MEPKGELLLETHFNENKVELIIQDTGSGIPQSQLEKLFVPFYTTKNQGTGLGLPLCLSIA 536 G++ + + KV + + ++G IP+ EK+F PF+TTK QGTGLGL +C I Sbjct: 186 TGENGKIKITSEDMYTKVRVSVWNSGPPIPEELKEKIFSPFFTTKTQGTGLGLSICRKII 245 Query: 537 ERHHGSIRVESQVGKGTAFIVSFPYVQEGR 566 E HG G FI P E R Sbjct: 246 EDEHGGKIWTENRENGVVFIFEIPKTPEKR 275 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.322 0.139 0.403 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 38,926,213 Number of extensions: 1544087 Number of successful extensions: 5433 Number of sequences better than 1.0e-05: 207 Number of HSP's gapped: 5225 Number of HSP's successfully gapped: 207 Length of query: 582 Length of database: 38,378,431 Length adjustment: 112 Effective length of query: 470 Effective length of database: 25,461,023 Effective search space: 11966680810 Effective search space used: 11966680810 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 119 (50.4 bits)