BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0303.0003.p (272 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value CAC3665 [R] COG1073 Hydrolases of the alpha/beta superfamily 50 5e-06 >CAC3665 [R] COG1073 Hydrolases of the alpha/beta superfamily Length = 265 Score = 49.7 bits (117), Expect = 5e-06, Method: Compositional matrix adjust. Identities = 56/251 (22%), Positives = 102/251 (40%), Gaps = 25/251 (9%) Query: 1 MPRNFELPAGEDAVLRCSHFPAQGEAK-SLVVVAHGYKGFKDWGMFPYVAEALSDEH-EV 58 M ++ E+ + + H P + K +VV+ HG+ G K F +V A E + Sbjct: 7 MQKSVEIKSKSLTLRGVLHMPLEAREKLPIVVIYHGFCGNKMGPHFIFVKLARELEKLGI 66 Query: 59 ISFNFSHAGIGEDLQNFTELEKFARNTYQREIKDMEILLSYLSQHHRFGSLPVFLLGHSR 118 + F AG GE F ++ T+ E+ D ++L Y+ + +LG S Sbjct: 67 ATIRFDFAGTGESDGEFVDM------TFSNEVYDANVILDYVKTLEFVDKDRISILGFSM 120 Query: 119 GGGDCLLYALDHPAELAGVISWNGTTNL------DLFTEEQKREMRETGRTHVLNGRTGQ 172 GG + A D E+ + W N+ D + ++ E+ E G V G+ Sbjct: 121 GGAIASVIAGDRKDEINTLCLWAPAGNMEQIILSDTYIGDKYDEIMEKGIYDVEGLLLGK 180 Query: 173 QMPLDAIIIEDLEQQAERYNILERMKQAAFPAVLIQGSEDGERLRRGSEQLIRFRPDIEW 232 + +ED+ ++ NI +R +++I G+ D SE+ + + Sbjct: 181 KF------LEDI----KKVNIFDRASAYNKQSLIIHGTSDEIVPLSTSERYLEMYGENTS 230 Query: 233 IQ-IPGGNHTF 242 ++ + G NH F Sbjct: 231 LELVEGANHIF 241 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.320 0.138 0.411 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 21,726,850 Number of extensions: 958125 Number of successful extensions: 1651 Number of sequences better than 1.0e-05: 1 Number of HSP's gapped: 1653 Number of HSP's successfully gapped: 1 Length of query: 272 Length of database: 38,378,431 Length adjustment: 105 Effective length of query: 167 Effective length of database: 26,268,361 Effective search space: 4386816287 Effective search space used: 4386816287 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 115 (48.9 bits)