BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0301.0014.p (2165 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value BH3697 [G] COG1523 Type II secretory pathway, pullulanase PulA a... 827 0.0 SP0268 [G] COG1523 Type II secretory pathway, pullulanase PulA a... 666 0.0 BH3264 [G] COG1523 Type II secretory pathway, pullulanase PulA a... 248 6e-65 SP1118 [G] COG1523 Type II secretory pathway, pullulanase PulA a... 195 6e-49 DR0405 [G] COG1523 Type II secretory pathway, pullulanase PulA a... 177 2e-43 >BH3697 [G] COG1523 Type II secretory pathway, pullulanase PulA and related glycosidases Length = 1072 Score = 827 bits (2135), Expect = 0.0, Method: Compositional matrix adjust. Identities = 437/846 (51%), Positives = 559/846 (66%), Gaps = 28/846 (3%) Query: 1 VDVPLKTDAQKIGFLVVNRSSGDKDGGDKTFRLLDRYNQLWIKQGDDHVYVSPFGEQPVS 60 +DV L DA +IGFLVVNR +GDKDG DKTF L + QL+I+QGDD VY +P+ + Sbjct: 199 LDVELADDANEIGFLVVNRENGDKDGDDKTFADLANHPQLFIRQGDDTVYTNPYYQTIEG 258 Query: 61 LVSAEILSTRKILLTFTLTDGLDAAGLKSLITVTDKEGTPVPVNAAAIKSTTSVEVDTAA 120 LVS E++S ++ L FT T L L I +TD G V + I +V + + Sbjct: 259 LVSGELISLTEMELRFTSTIWLSEDDLAEQIHLTDTNGASVAFDHVTIHDEKTV-ILHGS 317 Query: 121 FALEKAPLSVAYSGKNVSASAGWRMIDEMYNYTGDDLGATYHPADQSATLKLWAPMASSV 180 F E AP V ++ K V A AGWR+ID +Y Y G+ LGA H D S+TL W+P+A V Sbjct: 318 FDAELAPYHVTFADKTVVARAGWRLIDALYGYDGE-LGAVLH-EDGSSTLTFWSPLADHV 375 Query: 181 VANVYSKTDAAQQV-GQVSLTLGDKGVWSAELKPSDLSKAAAAEDVRGYYYQYEVTNNGV 239 +Y K D + + + +T GD+GVW L S+ +++ GYYY Y++ +G Sbjct: 376 SVVLYDKDDQYRIIKDDIDMTKGDQGVWQVTLH----SENTGLDNLAGYYYHYKIERDGD 431 Query: 240 TKPVLDPYAKSMAVFTVDSTGAAGPDGDTVGKAAIVDLSGTNPPDYRAGDIPGYEKREDA 299 TK LDPYAKSMA + + + + +GKAAIVD S P + +I G+EKREDA Sbjct: 432 TKLGLDPYAKSMAAWNNEGSYS-------IGKAAIVDPSSIGP-ELDFAEIEGFEKREDA 483 Query: 300 VIYEVHVRDFTSDVSIESELGGERWGSYAAFAKKLDYIKSLGITHIQLMPVMAWYNGDET 359 +IYEVHVRDFTSD IE EL + +G++A+F KLDYI+ LG+THIQL+PVM++Y GDE Sbjct: 484 IIYEVHVRDFTSDPGIEEELTAQ-FGTFASFVDKLDYIEDLGVTHIQLLPVMSYYWGDEL 542 Query: 360 RMTHRESGYSTQNNEYNWGYDPHSYFSPDGAYSQQPADPEERIKELKGLIDAVHEAGMGV 419 RE YS+ N YNWGYDPHSYFS G YS+ P DPE RIKE K LID +H GMGV Sbjct: 543 ANHERELAYSSSGNNYNWGYDPHSYFSLSGMYSENPEDPELRIKEFKHLIDEIHRRGMGV 602 Query: 420 ILDVVYTHMAKKDFLDDIVPNYYAFQDAX-XXXXXXXXXXLATSHKMAEKLMVDSVKYWF 478 ILDVVY H A + +D++PNYY F DA L T+HKM+ +++VDS+ YW Sbjct: 603 ILDVVYNHTAAVEIFEDLMPNYYHFMDADGTPRTSFGGGRLGTTHKMSRRILVDSITYWV 662 Query: 479 SEYKIDGMRWDMMGXXXXXXXXXXXXXXXXINPKALFIGEGWKTFAGDASDPALAGKGAD 538 EYK+DG R+DMMG +NP + IGEGW+T+ GD + AD Sbjct: 663 EEYKVDGFRFDMMGDHDAETIQIAYDRAKELNPNIVMIGEGWRTYVGDEHGGDIM--PAD 720 Query: 539 QAWMDKTDSVGVFSDEFRNELKSGYGSEGEPRFITGGARSIAAIFNNITARPSNIPADDP 598 Q WM T+SVGVFSDEFRNELKSG+GSEG+PRFITGGAR+I IF+N+TA+P N A +P Sbjct: 721 QDWMQHTESVGVFSDEFRNELKSGFGSEGQPRFITGGARNIEQIFDNLTAQPHNFTATNP 780 Query: 599 GDVVPYIEAHDNLTLHDVIAQTIKKDPSIPANELEIQQRIRLGNLLEMTSQGTAFIQAGQ 658 GDVVPYIEAHDNLTLHDVIAQ+I+KDP ++ EI +RIRLGN + +T+QGTAF+ AGQ Sbjct: 781 GDVVPYIEAHDNLTLHDVIAQSIRKDPEY--HQEEIHKRIRLGNTMVLTAQGTAFLHAGQ 838 Query: 659 EYGRTKQWKA--AGVPEQKYTEMKDANGQSF--GYFIHDSYDSSDAVNMFDWAKATDEVN 714 E+GRTKQ++A G K T M D NG+ F YFIHDSYDSSDA+N FDW KAT++ Sbjct: 839 EFGRTKQFRADTGGQAPYKSTYMTDENGKPFVYPYFIHDSYDSSDAINKFDWGKATNKEK 898 Query: 715 YPVQNTTRAYTSGLIQLRKSTDAFRLGDKSLVDTNVNLITAPEIKATDLVIGYKNKATDG 774 YP+ + TR YT+GLI+LR+ST+AF G V NV L+ PEI TDLVIGY+ +TD Sbjct: 899 YPIHHLTRTYTAGLIELRRSTNAFTHGTLVDVQANVRLLDIPEISETDLVIGYRATSTDN 958 Query: 775 TGLYYVFMNGDDAARTLTLSEDLTGGQVLVDNDQAGVTAIPAESQSGFTLTSTSITLDPL 834 TG YYVF+N D RTL+L++DLT G V+VD+D+AG+ A+ SGF LT IT+DPL Sbjct: 959 TGTYYVFVNADTKERTLSLTDDLTEGVVIVDSDEAGIVAV--SDPSGFDLTPNEITIDPL 1016 Query: 835 TAVVIR 840 TAV+IR Sbjct: 1017 TAVIIR 1022 Score = 57.0 bits (136), Expect = 3e-07, Method: Compositional matrix adjust. Identities = 29/62 (46%), Positives = 42/62 (67%), Gaps = 5/62 (8%) Query: 1 VDVPLKTDAQKIGFLVVNRSSGDKDGGDKTFRLLD-RYNQLWIKQGDDHVYVSPFGEQPV 59 VD+ LK +A+ IGFLV+N ++GDKDG DK +LL ++WIK+G D V++ +PV Sbjct: 89 VDIELKENAENIGFLVLNTATGDKDGNDKHVQLLSPEMKEIWIKEGSDDVFLY----EPV 144 Query: 60 SL 61 L Sbjct: 145 DL 146 >SP0268 [G] COG1523 Type II secretory pathway, pullulanase PulA and related glycosidases Length = 1280 Score = 666 bits (1718), Expect = 0.0, Method: Compositional matrix adjust. Identities = 367/865 (42%), Positives = 498/865 (57%), Gaps = 42/865 (4%) Query: 1 VDVPLKTDAQKIGFLVVNRSSGDKDGGDKTFRL-------LDRYNQLWIKQGDDHVYVSP 53 +D+PL A++ GFL+++ S K G D R L ++Q+++K D+ +Y +P Sbjct: 292 IDIPLNEAAREFGFLLLDES---KQGDDVKIRKENYKFTDLKNHSQIFLKDDDESIYTNP 348 Query: 54 FGEQPVSLVSAEILSTRKILLTFTLTDGLDAAGLKSLITVTDKEGTPVPVNAAAIKSTTS 113 + + + A+ + T I +F+ G + +T+ G V + AI Sbjct: 349 YYVHDIRMTGAQHVGTSSIESSFSTLVGAKKEDILKHSNITNHLGNKVTITDVAIDEAGK 408 Query: 114 VEVDTAAFALEKAPLSVAYSGKNVSASAGWRMIDEMYNYTGDDLGATYHPADQSATLKLW 173 + F+ K P +V+Y+ + WR+ DE Y+Y G LGA + L LW Sbjct: 409 KVTYSGDFSDTKHPYTVSYNSDQFTTKTSWRLKDETYSYDGK-LGADLKEEGKQVDLTLW 467 Query: 174 APMASSVVANVYSKTDAAQQVGQVSLTLGDKGVWSAELKPSDLSKAAAAEDVRGYYYQYE 233 +P A V VY K D + VG V+L G++G W L D + D GYYYQY+ Sbjct: 468 SPSADKVSVVVYDKNDPDKVVGTVALEKGERGTWKQTL---DSTNKLGITDFTGYYYQYQ 524 Query: 234 VTNNGVTKPVLDPYAKSMAVFTVDSTGAAGPDGDTVGKAAIVDLSGTNPPDYRAGDIPGY 293 + G T LDPYAKS+A + D + D V KAA VD + P D G I + Sbjct: 525 IERQGKTVLALDPYAKSLAAWNSDDSKID--DAHKVAKAAFVDPAKLGPQDLTYGKIHNF 582 Query: 294 EKREDAVIYEVHVRDFTSDVSIESELGGERWGSYAAFAKKLDYIKSLGITHIQLMPVMAW 353 + REDAVIYE HVRDFTSD +I +L + +G++ AF +KLDY+K LG+THIQL+PV+++ Sbjct: 583 KTREDAVIYEAHVRDFTSDPAIAKDLT-KPFGTFEAFIEKLDYLKDLGVTHIQLLPVLSY 641 Query: 354 YNGDETRMTHRESGYSTQNNEYNWGYDPHSYFSPDGAYSQQPADPEERIKELKGLIDAVH 413 Y +E + R S Y++ N+ YNWGYDP +YFS G YS P +PE+RI E K LI+ +H Sbjct: 642 YFVNELKNHERLSDYASSNSNYNWGYDPQNYFSLTGMYSSDPKNPEKRIAEFKNLINEIH 701 Query: 414 EAGMGVILDVVYTHMAKKDFLDDIVPNYYAFQDAXXXXXXXXXX-XLATSHKMAEKLMVD 472 + GMG ILDVVY H AK D +D+ PNYY F DA L T+H M ++L++D Sbjct: 702 KRGMGAILDVVYNHTAKVDLFEDLEPNYYHFMDADGTPRTSFGGGRLGTTHHMTKRLLID 761 Query: 473 SVKYWFSEYKIDGMRWDMMGXXXXXXXXXXXXXXXXINPKALFIGEGWKTFAGDASDPAL 532 S+KY YK+DG R+DMMG +NP + +GEGW+T+AGD + P Sbjct: 762 SIKYLVDTYKVDGFRFDMMGDHDAASIEEAYKAARALNPNLIMLGEGWRTYAGDENMPT- 820 Query: 533 AGKGADQAWMDKTDSVGVFSDEFRNELKSGYGSEGEPRFITGGARSIAAIFNNITARPSN 592 K ADQ WM TD+V VFSD+ RN LKSGY +EG+P FITGG R + IF N+ A+P+N Sbjct: 821 --KAADQDWMKHTDTVAVFSDDIRNNLKSGYPNEGQPAFITGGKRDVNTIFKNLIAQPTN 878 Query: 593 IPADDPGDVVPYIEAHDNLTLHDVIAQTIKKDPSIPANELEIQQRIRLGNLLEMTSQGTA 652 AD PGDV+ YI AHDNLTL D+IAQ+IKKDPS N EI +R+RLGNL+ +T+QGT Sbjct: 879 FEADSPGDVIQYIAAHDNLTLFDIIAQSIKKDPSKAENYAEIHRRLRLGNLMVLTAQGTP 938 Query: 653 FIQAGQEYGRTKQWKAAG---------VPEQKYTEMKDANGQSFG--YFIHDSYDSSDAV 701 FI +GQEYGRTKQ++ VP + + ++D +G F YFIHDSYDSSDAV Sbjct: 939 FIHSGQEYGRTKQFRDPAYKTPVAEDKVPNKSHL-LRDKDGNPFDYPYFIHDSYDSSDAV 997 Query: 702 NMFDWAKATDEVNYPVQNTTRAYTSGLIQLRKSTDAFRLGDKSLVDTNVNLITAP---EI 758 N FDW KATD YP +R Y GLI LR+STDAFRL + V+LIT P + Sbjct: 998 NKFDWTKATDGKAYPENVKSRDYMKGLIALRQSTDAFRLKSLQDIKDRVHLITVPGQNGV 1057 Query: 759 KATDLVIGYKNKATDGTGLYYVFMNGDDAARTLTLSE---DLTGGQVLVDNDQAGVTAIP 815 + D+VIGY+ A +G +Y VF+N D+ AR L L +VL D +QAG I Sbjct: 1058 EKEDVVIGYQITAPNG-DIYAVFVNADEKAREFNLGTAFAHLRNAEVLADENQAGPVGI- 1115 Query: 816 AESQSGFTLTSTSITLDPLTAVVIR 840 + G T + L+ LTA V+R Sbjct: 1116 -ANPKGLEWTEKGLKLNALTATVLR 1139 >BH3264 [G] COG1523 Type II secretory pathway, pullulanase PulA and related glycosidases Length = 717 Score = 248 bits (634), Expect = 6e-65, Method: Compositional matrix adjust. Identities = 228/809 (28%), Positives = 338/809 (41%), Gaps = 144/809 (17%) Query: 56 EQPVSLV--SAEILSTRKILLTFTLTDGLDAAGLKSLITVTDKEGTPVPVNAAAIKSTTS 113 EQ V + SAEI++ +++ FT+T + V +KEGT VP +IKS Sbjct: 6 EQAVKCLFHSAEIVTLHTVMIHFTVTKE------QVCPFVVEKEGTTVPF---SIKSWQE 56 Query: 114 VEVDTAAFALEKAPLSVAYSGK------------NVSASAGWRMIDEMYNYTGDDLGATY 161 E AF PL + K V D+ Y Y G+ LGA Y Sbjct: 57 WERGAIAFIQFDDPLELGVEYKLRQKNHEQTHLIKVGRVVRTEAFDQQYVYNGE-LGALY 115 Query: 162 HPADQSATLKLWAPMASSVVANVYSKTDAAQQVGQVSLTLGDKGVWSAELKPSDLSKAAA 221 + + +W P+A+ V +Y + + + + GVWS L Sbjct: 116 --SHERTVFAVWTPVATEVSLLLYENWHTLEAI-EYQMKRSPNGVWSVSLDG-------- 164 Query: 222 AEDVRGYYYQYEVTNNGVTKPVLDPYAKSMAVFTVDSTGAAGPDGDTVGKAAIVDLSGTN 281 D+ Y Y Y NG K +DPYAKS+ V +G+ K I DL T+ Sbjct: 165 --DLHQYCYLYRAKVNGKWKTTVDPYAKSVTV-----------NGE---KGVIFDLKRTD 208 Query: 282 PPDY-RAGDIPGYEKREDAVIYEVHVRDFTSDVSIESELGGERWGSYAAFAKK------- 333 P + IP E +IYE+H+RD T + G + G+Y + A++ Sbjct: 209 PASWPTVHPIPP----EHTIIYELHIRDAT----VYPGSGHHKKGTYLSLAEQNTCGPWG 260 Query: 334 ----LDYIKSLGITHIQLMPVMAWYNGDETRMTHRESGYSTQNNEYNWGYDPHSYFSPDG 389 L YI+ LG+TH++L+PV + + DE + + EYNWGYD YF+P+G Sbjct: 261 ESTGLSYIRELGVTHLELLPVADYGSVDE----------ANREKEYNWGYDVVHYFAPEG 310 Query: 390 AYSQQPADPEERIKELKGLIDAVHEAGMGVILDVVYTH--MAKKDFLDDIVPNYYAFQDA 447 +Y+ P D R+KELK LI + VILDVV+ H + K+ + +VP YY D Sbjct: 311 SYASDPYDGVTRVKELKQLIAQCQRMDLRVILDVVFNHVYVMKESPFEKLVPGYYFRYDG 370 Query: 448 XXXXX--XXXXXXLATSHKMAEKLMVDSVKYWFSEYKIDGMRWDMMGXXXXXXXXXXXXX 505 +A+ M K +VD V YW SEY++DG R+D+MG Sbjct: 371 EGNLADGTGVGNDIASERLMVRKFIVDCVTYWASEYRVDGFRFDLMGIIDQMTMKEVKRA 430 Query: 506 XXXINPKALFIGEGWKTFAGDASDPALAGKGADQAWMDKTDSVGVFSDEFRNELKSGYGS 565 IN + L +GEGW D P + A + F D FR+ +K Sbjct: 431 LAPINRQILLLGEGW-----DLETPLENDQKTTLAQARAVPFLHFFHDTFRDAVKGSTFD 485 Query: 566 EGEPRFITGGARSIAAIFNNITARPSNIPADDPGDVVPYIEAHDNLTLHDVIAQTIKKDP 625 + F G + ++ + D P + Y+EAHDN TL D K Sbjct: 486 LNKGGFCAGNIELKETLIASMLGHEAQF--DRPSQAIQYVEAHDNHTLWD------KLQF 537 Query: 626 SIPANELEIQQRI-RLGNLLEMTSQGTAFIQAGQEYGRTKQWKAAGVPEQKYTEMKDANG 684 P E E+ + RL + +TSQG F+ GQE+ RTK +G Sbjct: 538 VFPDEEEEVLRACHRLATAIVLTSQGIPFLHGGQEFFRTK------------------HG 579 Query: 685 QSFGYFIHDSYDSSDAVNMFDWAKATDEVNYPVQNTTRAYTSGLIQLRKSTDAFRLGDKS 744 + +SY+S D +N DW++ + Y GLI LRK+ AF S Sbjct: 580 EG------NSYNSPDWINQLDWSRRA------IYAADVEYVKGLIALRKAHRAFCFSTFS 627 Query: 745 LVDTNVNLITAPEIKATDLVIGYKNKATDGTGLY---YVFMN--GDDAARTLTLSEDLTG 799 V+T++ + P VIG+ G + V N GD L S + Sbjct: 628 EVNTHMCPLKTP-----SEVIGFHYHHIAKWGPWDEMVVLHNGGGDKVDIRLPASGEW-- 680 Query: 800 GQVLVDNDQAGVTAIPAESQSGFTLTSTS 828 +V+VD +AG+ +P + +G T++ S Sbjct: 681 -EVIVDGKKAGL--VPLYTFNGHTVSVQS 706 Score = 65.9 bits (159), Expect = 7e-10, Method: Compositional matrix adjust. Identities = 82/336 (24%), Positives = 142/336 (42%), Gaps = 49/336 (14%) Query: 1289 LDYLQKLGINTLWITPVVDNIDFNKGADFGGTQYGY---HGYWAKDFTQLDEHLG--DMA 1343 L Y+++LG+ L + PV D ++ +GY H + + D + G + Sbjct: 265 LSYIRELGVTHLELLPVADYGSVDEANREKEYNWGYDVVHYFAPEGSYASDPYDGVTRVK 324 Query: 1344 TFKELIEKAHDKGIKIMVDVVLNHAGYGLKAEDNYPGVTAEDKARFDGMLRKDGVSADTD 1403 K+LI + +++++DVV NH Y +K E + + R+DG +G AD Sbjct: 325 ELKQLIAQCQRMDLRVILDVVFNHV-YVMK-ESPFEKLVPGYYFRYDG----EGNLADGT 378 Query: 1404 PIKGELAGLPDFKTEDPAVRQTLIGWQTGWLDNARTERGDTIDYFRVDTVKHVDSTTWKA 1463 + ++A +E VR+ ++ T W R +D FR D + +D T K Sbjct: 379 GVGNDIA------SERLMVRKFIVDCVTYWASEYR------VDGFRFDLMGIIDQMTMKE 426 Query: 1464 FKNALTAIDPAFKLTGEYFG--GTLDNDGG-NLHSGQMDGLLDF---GFKNAAK------ 1511 K AL I+ L GE + L+ND L + L F F++A K Sbjct: 427 VKRALAPINRQILLLGEGWDLETPLENDQKTTLAQARAVPFLHFFHDTFRDAVKGSTFDL 486 Query: 1512 ---DFTDGKISAVDTYLQH---REAQMDNTGMMAQFLSSHDENGFLSN----YVDGDKSK 1561 F G I +T + EAQ D Q++ +HD + + D ++ Sbjct: 487 NKGGFCAGNIELKETLIASMLGHEAQFDRPSQAIQYVEAHDNHTLWDKLQFVFPDEEEEV 546 Query: 1562 L----KIAAALQITAKGQPVIYYGEELGRSGANSGD 1593 L ++A A+ +T++G P ++ G+E R+ G+ Sbjct: 547 LRACHRLATAIVLTSQGIPFLHGGQEFFRTKHGEGN 582 >SP1118 [G] COG1523 Type II secretory pathway, pullulanase PulA and related glycosidases Length = 759 Score = 195 bits (496), Expect = 6e-49, Method: Compositional matrix adjust. Identities = 180/622 (28%), Positives = 272/622 (43%), Gaps = 92/622 (14%) Query: 146 IDEMYNYTGDDLGATYHPADQSATLKLWAPMASSVVANVYS---------KTDAAQQVGQ 196 D + Y G+ LG D A LW+P A+ V VY KT ++ Sbjct: 122 FDHHWGYQGE-LGCRV--EDNQAHFSLWSPTATEVQVVVYESAANDAPVWKTFEMKRGNS 178 Query: 197 VSLTLGDK--GVWSAELKPSDLSKAAAAEDVRGYYYQYEVTNNGVTKPVLDPYAKSMAVF 254 S D GVWS +++ ED+ G YQY+V DPY Sbjct: 179 YSYNHKDNTIGVWSLDVE----------EDLVGKTYQYQVQFPHHQTLTRDPY------- 221 Query: 255 TVDSTGAAGPDGDTVGKAAIVDLSGTNPPDYRAGDIPGY--EKREDAVIYEVHVRDFTSD 312 T A PDG + V+ N + + G + E AVI E+H+RD T Sbjct: 222 ----TIATSPDGKRSAILSHVEKQVENF-EVKHGSEATWRLENPCKAVICEMHIRDLTKS 276 Query: 313 VS--IESELGGERWGSYAA-------FAKKLDYIKSLGITHIQLMPVMAWYNGDETRMTH 363 + ++ L G G+ A + DYIK LG ++QL P+ + + Sbjct: 277 PTSGVDEHLRGTFLGAAQAGTVNQYGQSTAFDYIKKLGYNYVQLQPIA------DRHKEY 330 Query: 364 RESGYSTQNNEYNWGYDPHSYFSPDGAYSQQPADPEERIKELKGLIDAVHEAGMGVILDV 423 E G N YNWGYDP +Y +P+ ++S P DP + I++LK ++ A H+AG+GVI+DV Sbjct: 331 DEDG----NVTYNWGYDPQNYNAPETSFSTNPDDPAQVIRDLKVMVQAYHDAGIGVIMDV 386 Query: 424 VYTH---MAKKDFLDDIVPNYYAFQ--DAXXXXXXXXXXXLATSHKMAEKLMVDSVKYWF 478 VY H + F VP+YY D A+ H+M K M+DS+ YW Sbjct: 387 VYNHTFSVVDAPF-QTTVPDYYYRMNPDGTFQNGTGVGNETASEHEMFRKYMIDSLLYWV 445 Query: 479 SEYKIDGMRWDMMGXXXXXXXXXXXXXXXXINPKALFIGEGWKTFAGDASDPALAGKGAD 538 EY IDG R+D+MG I+ + GEGW G A P K D Sbjct: 446 QEYNIDGFRFDLMGIHDVKTMQMIRQSLDEIDSNIILYGEGWDMGTGLA--PYDKAK-KD 502 Query: 539 QAWMDKTDSVGVFSDEFRNELKSG--YGSEGEPRFITGGARSIAAIFNNITARPSNIPAD 596 A+ + ++G F+D R+ +K G YG+ + F++G A + I Sbjct: 503 NAY--QMPNIGFFNDNQRDAVKGGEVYGA-IKSGFVSGAATE-PILAKAILGSRELGSYT 558 Query: 597 DPGDVVPYIEAHDNLTLHDVIAQTIKKDPSIPANELEIQQRIRLGNLLEMTSQGTAFIQA 656 P V+ Y+EAHDN LHD++A T+ D S +I +++ + + QG AF++ Sbjct: 559 HPNQVLNYVEAHDNYNLHDLLA-TLHPDQS----SEQIMRKVETATAMNLLMQGMAFMEI 613 Query: 657 GQEYGRTKQWKAAGVPEQKYTEMKDANGQSFGYFIHDSYDSSDAVNMFDWAKATDEVNYP 716 GQE+GRTK E + + + A +SY++ D+VN +W + Sbjct: 614 GQEFGRTKLVATGENGELTHDDRERA---------MNSYNAPDSVNQVNWNLINE----- 659 Query: 717 VQNTTRAYTSGLIQLRKSTDAF 738 + + + +I+L+ T AF Sbjct: 660 -RQDSIEFIRQVIRLKTKTGAF 680 >DR0405 [G] COG1523 Type II secretory pathway, pullulanase PulA and related glycosidases Length = 910 Score = 177 bits (449), Expect = 2e-43, Method: Compositional matrix adjust. Identities = 232/908 (25%), Positives = 366/908 (40%), Gaps = 232/908 (25%) Query: 5 LKTDAQKIGFLVVNRSSGDKDGGDKTFRLLDRYNQLWIKQG---------DDHVY-VSPF 54 +KTD +K+GF+V DKD G ++ NQLWI G D V V Sbjct: 1 MKTDWKKLGFIV--HKGDDKDPGPDGTLTREQGNQLWIVSGNPTPSTTRPDTRVRTVGDL 58 Query: 55 GEQPVSLVSAEILSTRKILL------------TFTLTDGLDAAGLKSLITVTDKEGTPVP 102 +Q ++S +++ + L T TL G D + + + EG P Sbjct: 59 KQQQAIMLSRDLIVVKPALAQPGAWLTLHVSPTATLKLGADGVSGGDTLALEEVEGGLTP 118 Query: 103 VNAAA-----------IKSTTSVEVDTAAFALEKAPLSVAYSGKNVSASA---GWRMIDE 148 A I++ ++ TA + + A SV GK + A+ W +D+ Sbjct: 119 ALQAKAPYLAGYKLLQIRAADRAKLPTA-LSGQLAVSSVLPGGKVLDATGVQTAW-ALDD 176 Query: 149 MYNYTGDDLGATYHPADQSATLKLWAPMASSVVANVYSKTDAAQQVGQVSLTLGDKGVWS 208 +Y Y G LG T+ T++LWAP A V +V S+ D Q+ + +T +GVW+ Sbjct: 177 LYAYAGP-LGVTWQ--GNKPTVRLWAPTAQDVKLHV-SRPDGTQER-TLPMTRDTQGVWT 231 Query: 209 AELKPSDLSKAAAAEDVRGYYYQYEV------TNNGVTKPVLDPYAKSMAVFTVDSTGAA 262 A + + +G Y++EV T N T V DPY+ ++ T +ST Sbjct: 232 A----------SGDQSWKGLAYRFEVRVFAPSTGNIETNLVTDPYSVAL---TRNST--- 275 Query: 263 GPDGDTVGKAAIVDLSGTN--PPDYRAGDIPGYEKREDAVIYEVHVRDFTS-DVSIESEL 319 + DLS + P + A P D YE+H+RDF++ D S+ + Sbjct: 276 --------HSVFADLSDNSQKPAGWNALKKPALRSFADLSFYELHLRDFSAADASVPA-- 325 Query: 320 GGERWGSYAAFAKK----LDYIKSL---GITHIQLMPVMAWYNGDETRMTHRESGYSTQ- 371 ER G+Y AF + + ++++L G+ + L+P +E + + G TQ Sbjct: 326 -AER-GTYLAFTRPGSDGMTHLRALAGAGLKAVHLLPTFDIATINEDKGQWQTPGDLTQF 383 Query: 372 --NNE--------------YNWGYDPHSYFSPDGAYSQQPADPEERIKELKGLIDAVHEA 415 N+E YNWGYDP+ P+G+Y+ PA ER KE + ++ +++ A Sbjct: 384 GPNSEEQQKAVNAVKDADAYNWGYDPYHSMVPEGSYAVNPA---ERTKEYRQMVMSLNAA 440 Query: 416 GMGVILDVVYTH-----MAKKDFLDDIVPNYYAFQDAXX-XXXXXXXXXLATSHKMAEKL 469 G+ V+ DVV+ H A++ LD IVP YY + AT H M +L Sbjct: 441 GLRVVQDVVFNHTAASGQAERSVLDKIVPGYYHRLNVNGGVENSTCCSNTATEHTMMRRL 500 Query: 470 MVDSVKYWFSEYKIDGMRWDMMGXXXXXXXXXXXXXXXXINP-------KALFI-GEGWK 521 MVD++ EYK+DG R+D+MG + P K +++ GEGW Sbjct: 501 MVDTLVLMAREYKVDGFRFDLMGHHMVADMQAARRALDALTPAKDGVDGKQIYLYGEGW- 559 Query: 522 TFAGDASDPALAGKGADQAWMDKTDS-VGVFSDEFRNELKSG--YGSEGEPRFITGGAR- 577 D + +GA+ ++ + +G F+D R+ L+ G +G E TG A Sbjct: 560 ----DFGEVQGNRRGANATQLNLYGAGIGTFNDRVRDALRGGSPFGGLQEQGVATGLATV 615 Query: 578 --------------SIAAI-------------FNNITARP--------SNIPAD---DPG 599 +A + F + + +P + PA P Sbjct: 616 PNGQAGNDSAEKWGQLADLVKLGLAGNLRDYRFTDHSGKPVTGGQLRYGDAPAGYAASPR 675 Query: 600 DVVPYIEAHDNLTLHDVIAQTIKKDPSIPANELEIQQRIRLGNLLE---MTSQGTAFIQA 656 + + Y AHDN TL D + PAN + R+R NL + QG F+ A Sbjct: 676 ETINYASAHDNQTLWDAVLL------KTPAN-MNSAARVRFQNLAHSYLLLGQGLPFVPA 728 Query: 657 GQEYGRTKQWKAAGVPEQKYTEMKDANGQSFGYFIHDSYDSSDAVNMFDWAKAT------ 710 G E R+K F DSY+S D N DW T Sbjct: 729 GDELLRSKS------------------------FDTDSYNSGDWFNALDWTGHTNGFGKG 764 Query: 711 ------DEVNYPVQNT--------------TRA--YTSGLIQLRKSTDAFRLGDKSLVDT 748 +E N+ + T TRA + L+++R S+ FRL + V Sbjct: 765 LPPAEKNEANWALYRTLLGDAALKVTPADITRASDHFRELLRVRSSSSLFRLDTAAQVQQ 824 Query: 749 NVNLITAP 756 +++ + AP Sbjct: 825 SLSFLPAP 832 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.314 0.132 0.380 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 159,797,986 Number of extensions: 6910720 Number of successful extensions: 13431 Number of sequences better than 1.0e-05: 118 Number of HSP's gapped: 13193 Number of HSP's successfully gapped: 137 Length of query: 2165 Length of database: 38,378,431 Length adjustment: 122 Effective length of query: 2043 Effective length of database: 24,307,683 Effective search space: 49660596369 Effective search space used: 49660596369 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (21.9 bits) S2: 124 (52.4 bits)