BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0301.0007.p (562 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value BH2903 [G] COG0366 Glycosidases 725 0.0 lin0223 [G] COG0366 Glycosidases 684 0.0 BS_yvdL [G] COG0366 Glycosidases 635 0.0 BH3868 [G] COG0366 Glycosidases 612 e-175 BS_ycdG [G] COG0366 Glycosidases 586 e-167 >BH2903 [G] COG0366 Glycosidases Length = 561 Score = 725 bits (1871), Expect = 0.0, Method: Compositional matrix adjust. Identities = 357/559 (63%), Positives = 428/559 (76%), Gaps = 6/559 (1%) Query: 1 MNRTFWKEAVVYQIYPRSFQDSNGDGIGDLQGIISRLDYLHKLGVDVVWLSPVYKSPNDD 60 M+ +WKE+VVYQIYPRSFQD NGDGIGD+ GIISRLDYL LGVDV+WLSPVY SPNDD Sbjct: 1 MSTQWWKESVVYQIYPRSFQDYNGDGIGDIPGIISRLDYLKTLGVDVIWLSPVYDSPNDD 60 Query: 61 NGYDISDYEDIMDEFGTLDDWEELLDGLHARGIKLMMDLVINHSSDEHAWFVESRSSKDN 120 NGYDI DY+ IMDEFGT+ DWE LL +H RG+KL+MDLV+NHSSDEHAWFVESR SKDN Sbjct: 61 NGYDIRDYKAIMDEFGTMADWETLLAEIHTRGMKLIMDLVVNHSSDEHAWFVESRKSKDN 120 Query: 121 PYRDYYIWRPGGPDGELPNNWSSIFSGPAWELDEKTGEYYLHLFSRKQPDLNWENPELRE 180 PYRD+YIWRPG DG+ PNNW+S FSG AW DE TGEYYLHLFS+KQPDLNWENP+LRE Sbjct: 121 PYRDFYIWRPG-KDGKEPNNWASNFSGSAWTYDETTGEYYLHLFSKKQPDLNWENPKLRE 179 Query: 181 ALYKMMAFWLDKGVDGLRMDVINMISKVEGLPSAHXXXXXXXXXXXXXXXXMNGPRVHEY 240 +Y+MM +WLDKG+DG RMDVIN ISKV+GLP A MNGP +H Y Sbjct: 180 KIYEMMTWWLDKGIDGFRMDVINFISKVDGLPDAEPQPGQPYVSGSNYF--MNGPNIHTY 237 Query: 241 LQEMNRKVLSKYNVMTVGETPGATVEDAILYTAEDRNELQMVFQFEHMDVDSGPGGKWNV 300 LQEM+ VL Y++MTVGE PG T+E A LYT E+RNEL MVFQFEH+ +D GP GKW++ Sbjct: 238 LQEMHENVLQHYDLMTVGEMPGVTLELAQLYTGEERNELNMVFQFEHVGLDQGPNGKWDL 297 Query: 301 TPWNLTKLRDILHKWQVGLADRGWNSLYLNNHDQPRMVSRFGNDREYRVISAKMLATLLH 360 P L L+ L +WQ GL D GWNSLY NNHDQPR+VSRFG+D+ YRV SAKMLATLLH Sbjct: 298 KPLELKDLKASLSRWQKGLQDIGWNSLYWNNHDQPRIVSRFGDDQSYRVESAKMLATLLH 357 Query: 361 TLKGTPYIYQGEEIGMTNVQFPQLEDYKDIEIHNMYKEKVTVGGGDHEAILKAIHIKGRD 420 +KGTP+IYQGEEIGMTNV+F +E Y+DIE NMYKEK G HE ++ +IH KGRD Sbjct: 358 CMKGTPFIYQGEEIGMTNVRFDSIEQYQDIETLNMYKEK-RAQGVPHETLMASIHAKGRD 416 Query: 421 NARTPMQWDSSPNAGFTEGTPWLKLNPRYEEINVEQALADPDSVFYYYQKLIRLRKNHPV 480 NARTPMQWD + + GFT+GTPWL++NP Y+EINV+QAL DP+S+FY+YQKLI+LRK H + Sbjct: 417 NARTPMQWDETKHGGFTDGTPWLEVNPNYKEINVKQALKDPNSIFYHYQKLIQLRKEHAI 476 Query: 481 MVYGDYELLLPEHEYIYAYTRTLEGERWLILLNFCDTPQTVDLLEELNAVTET-IISNYP 539 +V+G Y+L+L + I+AY RT G+ L++ NF + E+ T ++SNY Sbjct: 477 LVHGSYDLILEDDPEIFAYKRTYNGQTLLVVCNFYGRITDFECPAEVVLSEPTLLLSNYD 536 Query: 540 EEQ-PAMDRETLRPYEARV 557 EE+ + LRPYEARV Sbjct: 537 EEENGSYTSFRLRPYEARV 555 >lin0223 [G] COG0366 Glycosidases Length = 553 Score = 684 bits (1765), Expect = 0.0, Method: Compositional matrix adjust. Identities = 335/559 (59%), Positives = 416/559 (74%), Gaps = 11/559 (1%) Query: 3 RTFWKEAVVYQIYPRSFQDSNGDGIGDLQGIISRLDYLHKLGVDVVWLSPVYKSPNDDNG 62 + +WK++VVYQIYP+SF DSNGDG+GD+QGII +LDYL +LGVDV+WLSPVY SP DDNG Sbjct: 4 KDWWKKSVVYQIYPKSFNDSNGDGVGDIQGIIEKLDYLKELGVDVIWLSPVYDSPQDDNG 63 Query: 63 YDISDYEDIMDEFGTLDDWEELLDGLHARGIKLMMDLVINHSSDEHAWFVESRSSKDNPY 122 YDI DY+ I +E+G + ++ LL GLH R +KL+MDLV+NH+SDEH WF ESR SKDNPY Sbjct: 64 YDIRDYQKIYEEYGDMATFDNLLQGLHDRDMKLVMDLVVNHTSDEHKWFEESRKSKDNPY 123 Query: 123 RDYYIWRPGGPDGELPNNWSSIFSGPAWELDEKTGEYYLHLFSRKQPDLNWENPELREAL 182 RDYY WR + E+ NNW SIFSGPAWELDEKTGEYYLHLFS+KQPDLNWENP+LR+ + Sbjct: 124 RDYYFWRE---ENEI-NNWGSIFSGPAWELDEKTGEYYLHLFSKKQPDLNWENPKLRQDV 179 Query: 183 YKMMAFWLDKGVDGLRMDVINMISKVEGLPSAHXXXXXXXXXXXXXXXXMNGPRVHEYLQ 242 Y MM FWLDKG+DG RMDVIN ISK P NGPR+HE+LQ Sbjct: 180 YNMMKFWLDKGIDGFRMDVINFISKNTAFPDG--PVAEGQIYGDAGNDFCNGPRIHEFLQ 237 Query: 243 EMNRKVLSKYNVMTVGETPGATVEDAILYTAEDRNELQMVFQFEHMDVDSGPGGKWNVTP 302 EMN++V SKY+VMTVGE PGA+ DA +YT NE+ M+F FEHM++DS KW++ P Sbjct: 238 EMNQEVTSKYDVMTVGEMPGASTTDAQIYTNPANNEVDMIFTFEHMNLDSDGDNKWDLKP 297 Query: 303 WNLTKLRDILHKWQVGLADRGWNSLYLNNHDQPRMVSRFGNDREYRVISAKMLATLLHTL 362 L L++ + +WQV L + GWNSLY NNHDQPR+VSRFGND +RV SAKMLAT LH + Sbjct: 298 IYLPDLKENMSEWQVALQENGWNSLYWNNHDQPRIVSRFGNDNRFRVRSAKMLATCLHMM 357 Query: 363 KGTPYIYQGEEIGMTNVQFPQLEDYKDIEIHNMYKEKVTVGGGDHEAILKAIHIKGRDNA 422 KGTPYIYQGEEIGMTNV F LE+Y+DIE NMYKE+ G HE+I+++I+ KGRDNA Sbjct: 358 KGTPYIYQGEEIGMTNVHFETLEEYRDIETLNMYKERKEQGHS-HESIMQSIYTKGRDNA 416 Query: 423 RTPMQWDSSPNAGFTEGTPWLKLNPRYEEINVEQALADPDSVFYYYQKLIRLRKNHPVMV 482 RTP QWD+S NAGFT GTPWLK+NPRY EIN E+AL +PDS+FYYYQ LI+LRK ++ Sbjct: 417 RTPYQWDNSENAGFTTGTPWLKVNPRYTEINNEEALKNPDSIFYYYQNLIKLRKTTEIIT 476 Query: 483 YGDYELLLPEHEYIYAYTRTLEGERWLILLNFCDTPQTVDLLEELNAVTE-TIISNYPEE 541 G+Y LLLP+ E I+AY R E E+ ++L NF + Q + LN + + ++++N Sbjct: 477 TGNYRLLLPKDEAIFAYERYTENEKLVVLCNFTEEEQVLTDENILNDIKKGSVLAN---N 533 Query: 542 QPAMDRETLRPYEARVCRL 560 P + TLRPYEA V ++ Sbjct: 534 VPDIIEGTLRPYEAIVYQI 552 >BS_yvdL [G] COG0366 Glycosidases Length = 561 Score = 635 bits (1638), Expect = 0.0, Method: Compositional matrix adjust. Identities = 303/556 (54%), Positives = 397/556 (71%), Gaps = 9/556 (1%) Query: 5 FWKEAVVYQIYPRSFQDSNGDGIGDLQGIISRLDYLHKLGVDVVWLSPVYKSPNDDNGYD 64 +WKEAVVYQIYPRSF D+NGDG GDLQG+I +LDY+ LG DV+WLSPV+ SP DDNGYD Sbjct: 4 WWKEAVVYQIYPRSFYDANGDGFGDLQGVIQKLDYIKNLGADVIWLSPVFDSPQDDNGYD 63 Query: 65 ISDYEDIMDEFGTLDDWEELLDGLHARGIKLMMDLVINHSSDEHAWFVESRSSKDNPYRD 124 ISDY+++ ++FGT +D +L+D +H RG+K++MDLV+NH+SDEHAWF ESR SKDNPYRD Sbjct: 64 ISDYKNMYEKFGTNEDMFQLIDEVHKRGMKIVMDLVVNHTSDEHAWFAESRKSKDNPYRD 123 Query: 125 YYIWRPGGPDGELPNNWSSIFSGPAWELDEKTGEYYLHLFSRKQPDLNWENPELREALYK 184 YY+W+ PDG PNNW SIFSG AW DE TG+YYLH FS+KQPDLNWEN +R +Y Sbjct: 124 YYLWKDPKPDGSEPNNWGSIFSGSAWTYDEGTGQYYLHYFSKKQPDLNWENEAVRREVYD 183 Query: 185 MMAFWLDKGVDGLRMDVINMISKVEGLPSAHXXXXXXXXXXXXXXXXMNGPRVHEYLQEM 244 +M FW+D+GVDG RMDVI ISK P NGPR+HE++QEM Sbjct: 184 VMRFWMDRGVDGWRMDVIGSISKYTDFPDYETDHSRSYIVGRYHS---NGPRLHEFIQEM 240 Query: 245 NRKVLSKYNVMTVGETPGATVEDAILYTAEDRNELQMVFQFEHMDVD---SGPGGKWNVT 301 NR+VLS Y+ MTVGE G+ +E+A YT R EL M+F FEHMD+D + P GKW + Sbjct: 241 NREVLSHYDCMTVGEANGSDIEEAKKYTDASRQELNMIFTFEHMDIDKEQNSPNGKWQIK 300 Query: 302 PWNLTKLRDILHKWQVGLADRGWNSLYLNNHDQPRMVSRFGNDREYRVISAKMLATLLHT 361 P++L L+ + +WQ GL + GWN+LY NHDQPR++SR+GNDR+ R AK AT+LH Sbjct: 301 PFDLIALKKTMTRWQTGLMNVGWNTLYFENHDQPRVISRWGNDRKLRKECAKAFATVLHG 360 Query: 362 LKGTPYIYQGEEIGMTNVQFPQLEDYKDIEIHNMYKEKVTVGGGDHEA-ILKAIHIKGRD 420 +KGTP+IYQGEEIGM N P LE Y D+EI N Y+E V E +KA+ IKGRD Sbjct: 361 MKGTPFIYQGEEIGMVNSDMP-LEMYDDLEIKNAYRELVVENKTMSEKEFVKAVMIKGRD 419 Query: 421 NARTPMQWDSSPNAGFTEGTPWLKLNPRYEEINVEQALADPDSVFYYYQKLIRLRKNHPV 480 +ARTPMQWD+ +AGFT G PW+ +N RY++INV+++L D DS+F+YYQKLI+LRK + + Sbjct: 420 HARTPMQWDAGKHAGFTAGDPWIPVNSRYQDINVKESLEDQDSIFFYYQKLIQLRKQYKI 479 Query: 481 MVYGDYELLLPEHEYIYAYTRTLEGERWLILLNFCDTPQTVDLLEEL-NAVTETIISNYP 539 M+YGDY+LL +++Y R GE+ L+++N + + EL + + +ISNYP Sbjct: 480 MIYGDYQLLQENDPQVFSYLREYRGEKLLVVVNLSEEKALFEAPPELIHERWKVLISNYP 539 Query: 540 EEQPAMDRETLRPYEA 555 +E+ + +L+PYEA Sbjct: 540 QERADLKSISLKPYEA 555 >BH3868 [G] COG0366 Glycosidases Length = 553 Score = 612 bits (1577), Expect = e-175, Method: Compositional matrix adjust. Identities = 305/561 (54%), Positives = 397/561 (70%), Gaps = 13/561 (2%) Query: 1 MNRTFWKEAVVYQIYPRSFQDSNGDGIGDLQGIISRLDYLHKLGVDVVWLSPVYKSPNDD 60 MNR +WKEAV YQIYPRSF DSNGDGIGDLQG+I +LDY+ LG+DV+W+ P+Y+SPNDD Sbjct: 1 MNRQWWKEAVAYQIYPRSFCDSNGDGIGDLQGVIEKLDYVKDLGIDVIWICPMYRSPNDD 60 Query: 61 NGYDISDYEDIMDEFGTLDDWEELLDGLHARGIKLMMDLVINHSSDEHAWFVESRSSKDN 120 NGYDISDY+DIMDEFGT++D+++LL +H RG+KL++DLVINH+SDEH WF+ESRSSK N Sbjct: 61 NGYDISDYQDIMDEFGTMEDFDQLLHEVHRRGMKLIIDLVINHTSDEHPWFIESRSSKVN 120 Query: 121 PYRDYYIWRPGGPDGELPNNWSSIFSGPAWELDEKTGEYYLHLFSRKQPDLNWENPELRE 180 P RD+YIWR DG PNNW SIF+G AWE D+ TG+YY+H+FSRKQPDLNWEN ++R+ Sbjct: 121 PKRDWYIWRDPKEDGSEPNNWESIFNGSAWEYDQATGQYYMHIFSRKQPDLNWENRDVRK 180 Query: 181 ALYKMMAFWLDKGVDGLRMDVINMISKVEGLPSAHXXXXXXXXXXXXXXXXMNGPRVHEY 240 ALY+M+ +WL+KG+DG R+D I+ I KV+GLP MN +HEY Sbjct: 181 ALYQMINWWLEKGIDGFRVDAISHIKKVKGLPDL--PNPKGLDYVPSFEGHMNREGIHEY 238 Query: 241 LQEMNRKVLSKYNVMTVGETPGATVEDAILYTAEDRNELQMVFQFEHMDV-DSGPGGKWN 299 LQE+ R+ +N+MTVGE G TVE A + ED M+FQFEH+ + D G G Sbjct: 239 LQELKRETFDHFNIMTVGEANGVTVEQADDWVGEDHGAFNMIFQFEHLGLWDKGVNG--- 295 Query: 300 VTPWNLTKLRDILHKWQVGLADRGWNSLYLNNHDQPRMVSRFGNDREYRVISAKMLATLL 359 +L +L+ I WQ GL +GWN+L+L NHDQPR VS +GND+ Y SAK L TL Sbjct: 296 --TVDLAELKSIFTTWQKGLEGKGWNALFLENHDQPRSVSTWGNDKGYWKESAKALGTLF 353 Query: 360 HTLKGTPYIYQGEEIGMTNVQFPQLEDYKDIEIHNMYKEKVTVGGGDHEAILKAIHIKGR 419 ++GTP+IYQG+EIGMTNV+FP +EDY D+ + N Y+ + T G HE I++ I KGR Sbjct: 354 FFMQGTPFIYQGQEIGMTNVRFPSIEDYDDVGMKNFYRIE-TEKGRSHEEIMEIIWQKGR 412 Query: 420 DNARTPMQWDSSPNAGFTEGT-PWLKLNPRYEEINVEQALADPDSVFYYYQKLIRLRKNH 478 DN+RTPMQW+ +AGF+ T W+ +NP Y++INVE+ L + DSV YY+K+I LRK+ Sbjct: 413 DNSRTPMQWNDQKHAGFSTATETWMGVNPNYKQINVEKQLKEEDSVLSYYKKMIELRKSS 472 Query: 479 PVMVYGDYELLLPEHEYIYAYTRTLEGERWLILLNFCDTPQTVDLLEELNAVTETIISNY 538 + VYG Y+L+LPEH+ I+AY RTLE E LIL+N + +L E ++ NY Sbjct: 473 DLFVYGSYDLVLPEHKQIFAYKRTLEKESALILVNMFADEVSFQFPLKLIGEKERVLGNY 532 Query: 539 PEEQPAMDRETLRPYEARVCR 559 + Q A D+ LRPYEARV R Sbjct: 533 -KTQGAKDK--LRPYEARVYR 550 >BS_ycdG [G] COG0366 Glycosidases Length = 464 Score = 586 bits (1511), Expect = e-167, Method: Compositional matrix adjust. Identities = 0/562 (0%), Positives = 0/562 (0%), Gaps = 7/562 (1%) Query: 1 MNRTFWKEAVVYQIYPRSFQDSNGDGIGDLQGIISRLDYLHKLGVDVVWLSPVYKSPNDD 60 M R+ A + + + S + D Sbjct: 1 MVRSQLSRADAPCLLTSLRRQLMAMFGFPHWQLKSTSTESGVVAPDERLPFAQTAVMGVQ 60 Query: 61 NGYDISDYEDIMDEFGTLDDWEELLDGLHARGIKLMMDLVINHSSDEHAWFVESRSSKDN 120 + + +M LD +L + + S + Sbjct: 61 HAVAMFGATVLMPILMGLDPNLSILMSGIGTLLFFFITGGRVPSYLGSSAAFVGVVIAAT 120 Query: 121 PYRDYYIWRPGGPDGELPNNWSSIFSGPAWELDEKTGEYYLHLFSRKQPDLNWENPELRE 180 + I + + K G ++ Sbjct: 121 GFNGQGINPNISIALG-GIIACGLVYTVIGLVVMKIGTRWIERLMPPVVTGAVVMAIGLN 179 Query: 181 ALYKMMAFWLDKGVDGLRMDVINMISKVEGLPSAHXXXXXXXXXXXXXXXXMNGPRVHEY 240 + D + + + + + Sbjct: 180 LAPIAVKSVSASAFDSWMAVMTVLCIGLVAVFTRGMIQRLLILVGLIVA---CLLYGVMT 236 Query: 241 LQEMNRKVLSKYNVMTVGETPGATVEDAILYTAEDRNELQMVFQFEHMDVDSGPGGK-WN 299 K + V + ++ Sbjct: 237 NVLGLGKAVDFTLVSHAAWFGLPHFSTPAFNGQAMMLIAPVAVILVAENLGHLKAVAGMT 296 Query: 300 VTPWNLTKLRDILHKWQVGLADRGWNSLYLNNHDQPRMVSRFGNDREYRVISAKMLATLL 359 + R + + + + + V V A + +L Sbjct: 297 GRNMDPYMGRAFVGDGLATMLSGSVGGSGVTTYAENIGVMAVTKVYSTLVFVAAAVIAML 356 Query: 360 HTLKGTPYIYQGEEIGMTNVQFPQLEDYKDIEIHNMYKEKVTVGGGDHEAILKAIHIKGR 419 + + V + ++ Sbjct: 357 LGFSPKFGALIHTIPAAVIGGASIVVFGLIAVAGARIWVQNRVDLSQNGNLIMVAVTLVL 416 Query: 420 DNARTPMQWDSSPNAGFTEGTPWLKLNPRYEEINVEQALADPDSVFYYYQKLIRLRKNHP 479 Sbjct: 417 476 Query: 480 VMVYGDYELLLPEHEYIYAYTRTLEGERWLILLNFCDTPQTVDLLEEL-NAVTETIISNY 538 Sbjct: 477 536 Query: 539 PEEQPAMDRETLRPYEARVCRL 560 Sbjct: 537 - 557 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.318 0.138 0.430 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 47,007,369 Number of extensions: 2227292 Number of successful extensions: 3593 Number of sequences better than 1.0e-05: 110 Number of HSP's gapped: 3273 Number of HSP's successfully gapped: 119 Length of query: 562 Length of database: 38,378,431 Length adjustment: 112 Effective length of query: 450 Effective length of database: 25,461,023 Effective search space: 11457460350 Effective search space used: 11457460350 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 118 (50.1 bits)