BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0301.0003.p (273 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value MA2013_2 [T] COG0642 Signal transduction histidine kinase 197 1e-50 ZbarA_1 [T] COG0642 Signal transduction histidine kinase 187 2e-47 barA_1 [T] COG0642 Signal transduction histidine kinase 187 2e-47 ZbarA_1 [T] COG0642 Signal transduction histidine kinase 187 2e-47 all0638_1 [T] COG0642 Signal transduction histidine kinase 184 1e-46 >MA2013_2 [T] COG0642 Signal transduction histidine kinase Length = 296 Score = 197 bits (502), Expect = 1e-50, Method: Compositional matrix adjust. Identities = 105/253 (41%), Positives = 151/253 (59%), Gaps = 12/253 (4%) Query: 19 EREILRXXXXXXXXXXXXXDFLAMMSHEIRTPMNGMIGMADXXXXXXXXXXQRTYAEILR 78 E ++L+ DFLA MSHEIRTPMN ++GM + QR Y ++ Sbjct: 4 EEQLLKAKEAAESANKAKGDFLANMSHEIRTPMNAVLGMLEMLLETSLTDEQREYLQLAH 63 Query: 79 SSSHSLLKILNDILDFSKMEAGKMPLLSEKFDLREMMESVIDLFTPKAEEKKLALRWWAD 138 +S+ SLL I++D+LDFSK+E K+ L F+L ++ +I+L + KA K L L + + Sbjct: 64 ASAESLLSIIDDVLDFSKIEQNKLELEQISFELESLISHIINLLSGKAGSKGLKLAFHIE 123 Query: 139 TSVPSMVTTDPSRLRQIIVNLVGNALKFTERGSVTLSVKNIQLPAS-----------PEY 187 +PS DP RL+Q++ N++GNA+KFT++G + LSV+ + +P+ E Sbjct: 124 KDLPSTFIGDPVRLKQVLFNILGNAIKFTKKGEIALSVE-VYMPSERNSGLSNSDFPEEV 182 Query: 188 LLEFSVRDTGVGIADSKLNLLFQSFSQLHPFINRKYGGTGLGLAICKQLVELMGGTIFVE 247 L F V+DTG+GI KL+ +F F Q + R+YGGTGLGLAI QLVELM G I+VE Sbjct: 183 ALLFKVKDTGIGIPPEKLSQIFDPFIQADASVTREYGGTGLGLAISSQLVELMEGRIWVE 242 Query: 248 SEEGRGSTFRFML 260 SE G+G TF F + Sbjct: 243 SEVGKGCTFYFTV 255 >ZbarA_1 [T] COG0642 Signal transduction histidine kinase Length = 525 Score = 187 bits (474), Expect = 2e-47, Method: Compositional matrix adjust. Identities = 99/223 (44%), Positives = 140/223 (62%), Gaps = 1/223 (0%) Query: 38 DFLAMMSHEIRTPMNGMIGMADXXXXXXXXXXQRTYAEILRSSSHSLLKILNDILDFSKM 97 +FLA MSHE+RTP+NG+IG QR + + S+++LL I+ND+LDFSK+ Sbjct: 295 EFLANMSHELRTPLNGVIGFTRLTLKTELTPTQRDHLNTIERSANNLLAIINDVLDFSKL 354 Query: 98 EAGKMPLLSEKFDLREMMESVIDLFTPKAEEKKLALRWWADTSVPSMVTTDPSRLRQIIV 157 EAGK+ L S F LR ++ V+ L + +K L L + VP V DP RL+QII Sbjct: 355 EAGKLILESIPFPLRSTLDEVVTLLAHSSHDKGLELTLNIKSDVPDNVIGDPLRLQQIIT 414 Query: 158 NLVGNALKFTERGSVTLSVKNIQLPASPEYLLEFSVRDTGVGIADSKLNLLFQSFSQLHP 217 NLVGNA+KFTE G++ + V+ L ++ + +E +RDTG+GI + + LFQ+F Q Sbjct: 415 NLVGNAIKFTENGNIDILVEKRAL-SNTKVQIEVQIRDTGIGIPERDQSRLFQAFRQADA 473 Query: 218 FINRKYGGTGLGLAICKQLVELMGGTIFVESEEGRGSTFRFML 260 I+R++GGTGLGL I ++LV MGG I S+ RGSTF F + Sbjct: 474 SISRRHGGTGLGLVITQKLVNEMGGDISFHSQPNRGSTFWFHI 516 >barA_1 [T] COG0642 Signal transduction histidine kinase Length = 525 Score = 187 bits (474), Expect = 2e-47, Method: Compositional matrix adjust. Identities = 99/223 (44%), Positives = 140/223 (62%), Gaps = 1/223 (0%) Query: 38 DFLAMMSHEIRTPMNGMIGMADXXXXXXXXXXQRTYAEILRSSSHSLLKILNDILDFSKM 97 +FLA MSHE+RTP+NG+IG QR + + S+++LL I+ND+LDFSK+ Sbjct: 295 EFLANMSHELRTPLNGVIGFTRLTLKTELTPTQRDHLNTIERSANNLLAIINDVLDFSKL 354 Query: 98 EAGKMPLLSEKFDLREMMESVIDLFTPKAEEKKLALRWWADTSVPSMVTTDPSRLRQIIV 157 EAGK+ L S F LR ++ V+ L + +K L L + VP V DP RL+QII Sbjct: 355 EAGKLILESIPFPLRSTLDEVVTLLAHSSHDKGLELTLNIKSDVPDNVIGDPLRLQQIIT 414 Query: 158 NLVGNALKFTERGSVTLSVKNIQLPASPEYLLEFSVRDTGVGIADSKLNLLFQSFSQLHP 217 NLVGNA+KFTE G++ + V+ L ++ + +E +RDTG+GI + + LFQ+F Q Sbjct: 415 NLVGNAIKFTENGNIDILVEKRAL-SNTKVQIEVQIRDTGIGIPERDQSRLFQAFRQADA 473 Query: 218 FINRKYGGTGLGLAICKQLVELMGGTIFVESEEGRGSTFRFML 260 I+R++GGTGLGL I ++LV MGG I S+ RGSTF F + Sbjct: 474 SISRRHGGTGLGLVITQKLVNEMGGDISFHSQPNRGSTFWFHI 516 Score = 187 bits (474), Expect = 2e-47, Method: Compositional matrix adjust. Identities = 99/223 (44%), Positives = 140/223 (62%), Gaps = 1/223 (0%) Query: 38 DFLAMMSHEIRTPMNGMIGMADXXXXXXXXXXQRTYAEILRSSSHSLLKILNDILDFSKM 97 +FLA MSHE+RTP+NG+IG QR + + S+++LL I+ND+LDFSK+ Sbjct: 295 EFLANMSHELRTPLNGVIGFTRLTLKTELTPTQRDHLNTIERSANNLLAIINDVLDFSKL 354 Query: 98 EAGKMPLLSEKFDLREMMESVIDLFTPKAEEKKLALRWWADTSVPSMVTTDPSRLRQIIV 157 EAGK+ L S F LR ++ V+ L + +K L L + VP V DP RL+QII Sbjct: 355 EAGKLILESIPFPLRSTLDEVVTLLAHSSHDKGLELTLNIKSDVPDNVIGDPLRLQQIIT 414 Query: 158 NLVGNALKFTERGSVTLSVKNIQLPASPEYLLEFSVRDTGVGIADSKLNLLFQSFSQLHP 217 NLVGNA+KFTE G++ + V+ L ++ + +E +RDTG+GI + + LFQ+F Q Sbjct: 415 NLVGNAIKFTENGNIDILVEKRAL-SNTKVQIEVQIRDTGIGIPERDQSRLFQAFRQADA 473 Query: 218 FINRKYGGTGLGLAICKQLVELMGGTIFVESEEGRGSTFRFML 260 I+R++GGTGLGL I ++LV MGG I S+ RGSTF F + Sbjct: 474 SISRRHGGTGLGLVITQKLVNEMGGDISFHSQPNRGSTFWFHI 516 >all0638_1 [T] COG0642 Signal transduction histidine kinase Length = 615 Score = 184 bits (466), Expect = 1e-46, Method: Compositional matrix adjust. Identities = 102/229 (44%), Positives = 144/229 (62%), Gaps = 5/229 (2%) Query: 38 DFLAMMSHEIRTPMNGMIGMADXXXXXXXX-XXQRTYAEILRSSSHSLLKILNDILDFSK 96 +FLA MSHE+RTP+NG++G A +R I+ LL ++NDILD SK Sbjct: 366 EFLANMSHELRTPLNGILGYAQILGRSKVLPDKERQGVNIIHQCGSHLLTLINDILDLSK 425 Query: 97 MEAGKMPLLSEKFDLREMMESVIDLFTPKAEEKKLALRWWADTSVPSMVTTDPSRLRQII 156 +EA K+ L S+ F L M+ V+++ + ++K + + D +P+ ++ D RLRQ++ Sbjct: 426 IEARKLELASQAFHLPSFMQGVVEICHIRVQQKGIEFHYEPDADLPAGISADEKRLRQVL 485 Query: 157 VNLVGNALKFTERGSVTLSVKNIQLPAS-PEYLLEFSVRDTGVGIADSKLNLLFQSFSQL 215 +NL+GNA+KFT+RGSV L V+ L S P L FS+ DTGVGIA + +N LFQ+F Q+ Sbjct: 486 INLLGNAIKFTDRGSVALRVQRTGLEESDPRTKLRFSIVDTGVGIASADINKLFQTFEQV 545 Query: 216 HPFINRKYGGTGLGLAICKQLVELMGGTIFVESEEGRGSTFRF--MLPF 262 RK GTGLGLAI +QLV+LMGG I V+S+ G GS F F +LP Sbjct: 546 GE-QKRKVEGTGLGLAISQQLVQLMGGQIQVKSQLGVGSEFFFEIILPL 593 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.321 0.137 0.389 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 17,230,006 Number of extensions: 678761 Number of successful extensions: 2576 Number of sequences better than 1.0e-05: 232 Number of HSP's gapped: 2243 Number of HSP's successfully gapped: 233 Length of query: 273 Length of database: 38,378,431 Length adjustment: 105 Effective length of query: 168 Effective length of database: 26,268,361 Effective search space: 4413084648 Effective search space used: 4413084648 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 115 (48.9 bits)