BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0294.0007.p (535 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value PA3637 [F] COG0504 CTP synthase (UTP-ammonia lyase) 598 e-170 AGc2984 [F] COG0504 CTP synthase (UTP-ammonia lyase) 546 e-155 YJR103w [F] COG0504 CTP synthase (UTP-ammonia lyase) 452 e-127 STM0803 [H] COG0521 Molybdopterin biosynthesis enzymes 452 e-127 YBL039c [F] COG0504 CTP synthase (UTP-ammonia lyase) 447 e-125 >PA3637 [F] COG0504 CTP synthase (UTP-ammonia lyase) Length = 542 Score = 598 bits (1541), Expect = e-170, Method: Compositional matrix adjust. Identities = 288/542 (53%), Positives = 382/542 (70%), Gaps = 13/542 (2%) Query: 1 VTKYIFVTGGVVSSLGKGITAASLGRLLKNRGLKVTIQKFDPYINVDPGTMSPYQHGEVF 60 +T+YIFVTGGVVSSLGKGI +ASL +L+ RGLK+T+ K DPYINVDPGTMSP+QHGEVF Sbjct: 1 MTRYIFVTGGVVSSLGKGIASASLAAILEARGLKITMLKLDPYINVDPGTMSPFQHGEVF 60 Query: 61 VTDDGAETDLDLGHYERFIDINLSKNSNVTTGKIYSSVISKERRGEYLGGTVQVIPHITN 120 VT DGAETDLDLGHYERF+ +++N+N TTG++Y V+ KERRG+YLG TVQVIPHIT+ Sbjct: 61 VTQDGAETDLDLGHYERFVRTTMTQNNNFTTGRVYMDVLRKERRGDYLGATVQVIPHITD 120 Query: 121 EIKERVFRAGRETGSDVVITEIGGTVGDIESLPFLEAIRQIKSDIGRENVMYIHVTLIPY 180 EIK R+ + + +DV + EIGGTVGDIES PFLEAIRQ++ +IG + M +H+TL+PY Sbjct: 121 EIKRRIIKGAGD--ADVALVEIGGTVGDIESQPFLEAIRQLRVEIGAKRAMLMHLTLVPY 178 Query: 181 IKAAGEVKTKPTQHSVKELRSIGIQPNVIVCRTEYALSDDMKAKIALFCDIDANAVVECR 240 I AGE KTKPTQHSVKELRSIG+QP+V+VCR+++ + + KIALF +++ AV+ Sbjct: 179 IATAGETKTKPTQHSVKELRSIGLQPDVLVCRSDHPIDVSSRRKIALFTNVEERAVIALE 238 Query: 241 DASTLYEVPLNLRDEGLDEIVVNHLKLTTPAPDMREWENMLGRIQKLERTVEIAIVGKYV 300 D T+Y +P L +GLD+IVV L D+ EW+ ++ ER V IA+VGKY+ Sbjct: 239 DVDTIYRIPSVLHAQGLDDIVVERFGLECGQADLSEWDRVVDAKLNPEREVTIAMVGKYM 298 Query: 301 ALHDAYLSVVESLSHAGFAANAEVKVRWVDAELVTDENXXXXXXXXXXXXXXXXXXDRGI 360 L DAY S++E+++HAG + +V +R++D+E + + RG+ Sbjct: 299 ELLDAYKSLIEAMTHAGIQSRTKVNLRYIDSEDIEQQGTSLLEGVDAILVPGGFGL-RGV 357 Query: 361 EGKISAIRYAREQSIPFFGICLGMQVSVIEYGRSILGLAGANSSEIDPATLHPLIDLLPE 420 EGKIS ++YARE IP+ GICLGMQV+VIEY R++LG + ANS+E D ++ HP++ L+ E Sbjct: 358 EGKISTVQYARENKIPYLGICLGMQVAVIEYARNVLGWSDANSTEFDKSSGHPVVGLITE 417 Query: 421 QKD----------IEDMGGTMRLGLYPCKLLPESLAMSCYDDELVYERHRHRYEFNNAYR 470 +D D+GGTMRLG C+L +L CY +++ ERHRHRYE NN Sbjct: 418 WQDATGATEIRTEASDLGGTMRLGAQECQLQTGTLVHDCYAKDVIVERHRHRYEVNNNLL 477 Query: 471 DEIEKAGLVISGTSPDGRLVEIVELPGHPWFLSVQFHPEFTSRPNRPQPLFREFVKASLT 530 ++E+AGL ISG S DG LVE+VE P HPWF++ QFHPEFTS P PLF FV A+L Sbjct: 478 PQLEQAGLKISGRSGDGALVEVVEAPEHPWFVACQFHPEFTSTPRDGHPLFSGFVNAALK 537 Query: 531 HS 532 +S Sbjct: 538 YS 539 >AGc2984 [F] COG0504 CTP synthase (UTP-ammonia lyase) Length = 218 Score = 546 bits (1406), Expect = e-155, Method: Compositional matrix adjust. Identities = 0/542 (0%), Positives = 0/542 (0%), Gaps = 13/542 (2%) Query: 1 VTKYIFVTGGVVSSLGKGITAASLGRLLKNRGLKVTIQKFDPYINVDPGTMSPYQHGEVF 60 + + + + + L R + + V G Sbjct: 37 MRNAGLTFSAIPADIDERALDEQLERDGASPEEVALELARAKALAVSALHPEALVLGCDQ 96 Query: 61 VTDDGAETDLDLGHYERFIDINLSKNSNVTTGKIYSSVISKERRGEYLGGTVQVIPHITN 120 G + LS + V + ++ + + ++ + Sbjct: 97 TMALGTRVYHKPKNMAEAATHLLSLSGKVHRLNSAAVLVHNGKVVWQTVSSAELAVRTLS 156 Query: 121 EIKERVFRAGRETGSDVVITEIGGTVGDIESLPFLEAIRQIKSDIGRENVMYIHVTLIPY 180 + + + F + +G + + Sbjct: 157 AEFVSRHLQRVG--EKALSSVGAYQLEREGIQLFTSIEGDYFTILGLPLLPLLSKLRDMD 214 Query: 181 IKAAGEVKTKPTQHSVKELRSIGIQPNVIVCRTEYALSDDMKAKIALFCDIDANAVVECR 240 Sbjct: 215 274 Query: 241 DASTLYEVPLNLRDEGLDEIVVNHLKL-TTPAPDMREWENMLGRIQKLERTVEIAIVGKY 299 Sbjct: 275 334 Query: 300 VALHDAYLSVVESLSHAGFAANAEVKVRWVDAELVTDENXXXXXXXXXXXXXXXXXXDRG 359 Sbjct: 335 394 Query: 360 IEGKISAIRYAREQSIPFFGICLGMQVSVIEYGRSILGLAGANSSEIDPATLHPLIDLLP 419 Sbjct: 395 - 453 Query: 420 EQ---KDIE------DMGGTMRLGLYPCKLLPESLAMSCYDDELVYERHRHRYEFNNAYR 470 Sbjct: 454 513 Query: 471 DEIEKAGLVISGTSPDGRLVEIVELPGHPWFLSVQFHPEFTSRPNRPQPLFREFVKASLT 530 Sbjct: 514 573 Query: 531 HS 532 Sbjct: 574 575 >YJR103w [F] COG0504 CTP synthase (UTP-ammonia lyase) Length = 564 Score = 452 bits (1164), Expect = e-127, Method: Compositional matrix adjust. Identities = 247/563 (43%), Positives = 343/563 (60%), Gaps = 37/563 (6%) Query: 3 KYIFVTGGVVSSLGKGITAASLGRLLKNRGLKVTIQKFDPYINVDPGTMSPYQHGEVFVT 62 KY+ V+GGV+S +GKG+ A+S G LLK GLKVT K DPY+N+D GTMSP +HGE FV Sbjct: 2 KYVVVSGGVISGIGKGVLASSTGMLLKTLGLKVTSIKIDPYMNIDAGTMSPLEHGECFVL 61 Query: 63 DDGAETDLDLGHYERFIDINLSKNSNVTTGKIYSSVISKERRGEYLGGTVQVIPHITNEI 122 DDG ETDLDLG+YER++ I LS++ N+TTGKIYS VIS+ERRG+YLG TVQ++PH+TN I Sbjct: 62 DDGGETDLDLGNYERYLGITLSRDHNITTGKIYSHVISRERRGDYLGKTVQIVPHLTNAI 121 Query: 123 KERVFRAGR----ETG--SDVVITEIGGTVGDIESLPFLEAIRQIKSDIGRENVMYIHVT 176 ++ + R + +TG DV I E+GGTVGDIES PF+EA+RQ + ++GREN IHV+ Sbjct: 122 QDWIQRVSKIPVDDTGLEPDVCIIELGGTVGDIESAPFVEALRQFQFEVGRENFALIHVS 181 Query: 177 LIPYIKAAGEVKTKPTQHSVKELRSIGIQPNVIVCRTEYALSDDMKAKIALFCDIDANAV 236 L+P I GE KTKPTQ ++K+LRS+G+ P++I CR L+ KIA+FC + V Sbjct: 182 LVPVIH--GEQKTKPTQAAIKDLRSLGLIPDMIACRCSEELNRSTIDKIAMFCHVGPEQV 239 Query: 237 VECRDASTLYEVPLNLRDEGLDEIVVNHLKLTTPAPDMRE----------WENMLGRIQK 286 V D ++ Y VPL L + + + + + LKL + + WENM + Sbjct: 240 VNVHDVNSTYHVPLLLLKQHMIDYLHSRLKLGEVPLTLEDKERGSQLLTNWENMTKNLDD 299 Query: 287 LERTVEIAIVGKYVALHDAYLSVVESLSHAGFAANAEVKVRWVDAELVTDENXXXXXXXX 346 + V+IA+VGKY L D+YLSV +SL HA ++++ WV+A + E Sbjct: 300 SDDVVKIALVGKYTNLKDSYLSVTKSLEHASMKCRRQLEILWVEASNLEPETQEVDKNKF 359 Query: 347 XXXXXXXXXXD----------RGIEGKISAIRYAREQSIPFFGICLGMQVSVIEYGRSIL 396 D RGIEG I A ++ARE +PF G+CLG+QV+ IE+ R+++ Sbjct: 360 HDSWNKLSSADGILVPGGFGTRGIEGMILAAKWARESGVPFLGVCLGLQVAAIEFARNVI 419 Query: 397 GLAGANSSE-IDPATLHP---LIDLLPEQKDIEDMGGTMRLGLYPCKLLPESLAMS---C 449 G ++S+E +D L P ++ +PE D E MGGTMRLGL P P S + Sbjct: 420 GRPNSSSTEFLDETLLAPEDQVVIYMPE-IDKEHMGGTMRLGLRPTIFQPNSEWSNIRKL 478 Query: 450 YDD-ELVYERHRHRYEFNNAYRDEIEKAGLVISGTSPDGRLVEIVELPGHPWFLSVQFHP 508 Y + V+ERHRHRYE N +++E G + G G+ EI EL GHP+++ Q+HP Sbjct: 479 YGEVNEVHERHRHRYEINPKIVNDMESRGFIFVGKDETGQRCEIFELKGHPYYVGTQYHP 538 Query: 509 EFTSRPNRPQPLFREFVKASLTH 531 E+TS+ P F V A+ H Sbjct: 539 EYTSKVLEPSRPFWGLVAAAPAH 561 >STM0803 [H] COG0521 Molybdopterin biosynthesis enzymes Length = 170 Score = 452 bits (1164), Expect = e-127, Method: Compositional matrix adjust. Identities = 0/528 (0%), Positives = 0/528 (0%), Gaps = 22/528 (4%) Query: 1 VTKYIFVTGGVVSSLGKGITAASLGRLLKNRGLKVTIQKFDPYINVDPGTMSPYQHGEVF 60 +++ ++ + L+ + Q+ + Sbjct: 1 MSQVSAEFIPTRIAILTVSSRRGEEDDTSGHYLRDSAQEAGHEVVAKAIVKENRYAIRAQ 60 Query: 61 VTDDGAETDLDLGHYERFIDINLSKNSNVTTGKIYSSVISKERRGEYLGGTVQVIPHITN 120 V+ A ++ + + + ++ + + ++ Sbjct: 61 VSAWIASDEVQVVLITGGTGLTEGDQAPEALLPLFDREVEGFGEVFRMLSFEEIGTATLQ 120 Query: 121 EIKERVFRAGRETGSDVVITEIGGTVGDIESLPFLEAIRQIKSDIGRENVMYIHVTLIPY 180 Sbjct: 121 SRAVAG---- 176 Query: 181 IKAAGEVKTKPTQHSVKELRSIGIQPNVIVCRTEYALSDDMKAKIALFCDIDANAVVECR 240 Sbjct: 177 236 Query: 241 DASTLYEVPLNLRDEGLDEIVVNHLKLTTPAPDMREWENMLGRIQKLERTVEIAIVGKYV 300 Sbjct: 237 ------ 290 Query: 301 ALHDAYLSVVESLSHAGFAANAEVKVRWVDAELVTDENXXXXXXXXXXXXXXXXXXDRGI 360 Sbjct: 291 -- 348 Query: 361 EGKISAIRYAREQSIPFFGICLGMQVSVIEYGRSILGLAGANSSEIDPATLHPLIDLLPE 420 Sbjct: 349 408 Query: 421 QKDIEDMGGTMRLGLYPCKLLPESLAMSCYDD-ELVYERHRHRYEFN-NAYRDEIEKAG- 477 Sbjct: 409 468 Query: 478 ----LVISGTSPDGRLVEIVELPGHPWFLSVQFHPEFTSRPNRPQPLF 521 Sbjct: 469 --- 513 >YBL039c [F] COG0504 CTP synthase (UTP-ammonia lyase) Length = 579 Score = 447 bits (1150), Expect = e-125, Method: Compositional matrix adjust. Identities = 242/558 (43%), Positives = 337/558 (60%), Gaps = 35/558 (6%) Query: 3 KYIFVTGGVVSSLGKGITAASLGRLLKNRGLKVTIQKFDPYINVDPGTMSPYQHGEVFVT 62 KY+ V+GGV+S +GKG+ A+S G L+K GLKVT K DPY+N+D GTMSP +HGE FV Sbjct: 2 KYVVVSGGVISGIGKGVLASSTGMLMKTLGLKVTSIKIDPYMNIDAGTMSPLEHGECFVL 61 Query: 63 DDGAETDLDLGHYERFIDINLSKNSNVTTGKIYSSVISKERRGEYLGGTVQVIPHITNEI 122 DDG ETDLDLG+YER++ + L+K+ N+TTGKIYS VI+KER+G+YLG TVQ++PH+TN I Sbjct: 62 DDGGETDLDLGNYERYLGVTLTKDHNITTGKIYSHVIAKERKGDYLGKTVQIVPHLTNAI 121 Query: 123 KERVFRAGR----ETG--SDVVITEIGGTVGDIESLPFLEAIRQIKSDIGRENVMYIHVT 176 ++ + R + +TG DV I E+GGTVGDIES PF+EA+RQ + +G+EN IHV+ Sbjct: 122 QDWIERVAKIPVDDTGMEPDVCIIELGGTVGDIESAPFVEALRQFQFKVGKENFALIHVS 181 Query: 177 LIPYIKAAGEVKTKPTQHSVKELRSIGIQPNVIVCRTEYALSDDMKAKIALFCDIDANAV 236 L+P I GE KTKPTQ ++K LRS+G+ P++I CR L KIA+FC + V Sbjct: 182 LVPVIH--GEQKTKPTQAAIKGLRSLGLVPDMIACRCSETLDKPTIDKIAMFCHVGPEQV 239 Query: 237 VECRDASTLYEVPLNLRDE----------GLDEIVVNHLKLTTPAPDMREWENMLGRIQK 286 V D ++ Y VPL L ++ LDEI + + + +W+ G + Sbjct: 240 VNVHDVNSTYHVPLLLLEQKMIDYLHARLKLDEISLTEEEKQRGLELLSKWKATTGNFDE 299 Query: 287 LERTVEIAIVGKYVALHDAYLSVVESLSHAGFAANAEVKVRWVDAELVTDENXXXXXXXX 346 TV+IA+VGKY L D+YLSV+++L H+ ++ ++WV+A + E Sbjct: 300 SMETVKIALVGKYTNLKDSYLSVIKALEHSSMKCRRKLDIKWVEATDLEPEAQESNKTKF 359 Query: 347 XXXXXXXXXXD----------RGIEGKISAIRYAREQSIPFFGICLGMQVSVIEYGRSIL 396 D RG EG + A R+ARE IPF G+CLG+Q++ IE+ RS+L Sbjct: 360 HEAWNMVSTADGILIPGGFGVRGTEGMVLAARWARENHIPFLGVCLGLQIATIEFTRSVL 419 Query: 397 GLAGANSSEIDPAT--LHPLIDLLPEQKDIEDMGGTMRLGLYPCKLLPE---SLAMSCYD 451 G ++S+E P + ++ +PE D E MGG+MRLGL P E S Y Sbjct: 420 GRKDSHSAEFYPDIDEKNHVVVFMPEI-DKETMGGSMRLGLRPTFFQNETEWSQIKKLYG 478 Query: 452 D-ELVYERHRHRYEFNNAYRDEIEKAGLVISGTSPDGRLVEIVELPGHPWFLSVQFHPEF 510 D V+ERHRHRYE N DE+E GL+ G G+ EI+EL HP++++ Q+HPE+ Sbjct: 479 DVSEVHERHRHRYEINPKMVDELENNGLIFVGKDDTGKRCEILELKNHPYYIATQYHPEY 538 Query: 511 TSRPNRPQPLFREFVKAS 528 TS+ P F V AS Sbjct: 539 TSKVLDPSKPFLGLVAAS 556 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.318 0.137 0.398 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 39,407,599 Number of extensions: 1701495 Number of successful extensions: 3616 Number of sequences better than 1.0e-05: 9 Number of HSP's gapped: 3579 Number of HSP's successfully gapped: 9 Length of query: 535 Length of database: 38,378,431 Length adjustment: 111 Effective length of query: 424 Effective length of database: 25,576,357 Effective search space: 10844375368 Effective search space used: 10844375368 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 118 (50.1 bits)