BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0291.0023.p (405 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value BH1790 [R] COG0523 Putative GTPases (G3E family) 488 e-138 SA0410 [R] COG0523 Putative GTPases (G3E family) 481 e-136 AGl3257 [R] COG0523 Putative GTPases (G3E family) 385 e-107 PA5535 [R] COG0523 Putative GTPases (G3E family) 375 e-104 BMEII0179 [R] COG0523 Putative GTPases (G3E family) 372 e-103 >BH1790 [R] COG0523 Putative GTPases (G3E family) Length = 395 Score = 488 bits (1257), Expect = e-138, Method: Compositional matrix adjust. Identities = 240/387 (62%), Positives = 297/387 (76%), Gaps = 2/387 (0%) Query: 4 IPVTVLSGYLGSGKTTLLNHILHNRDGLKVAVIVNDMSEVNVDAGLVKSGNTLSRTEEKL 63 IPVTVLSGYLG+GKTT+LNH+L N++G+KVAVIVNDMSEVN+DA LVK G +RT+EKL Sbjct: 6 IPVTVLSGYLGAGKTTVLNHVLANKEGMKVAVIVNDMSEVNIDATLVKQGG-FTRTDEKL 64 Query: 64 VEMSNGCICCTLRDDLLREVHTLAAEGRFDYILIESSGISEPVPVAQTFTYANPELDIDL 123 VE+ NGCICCTLR+DL+ EV LA G DYILIES+GISEP+PVAQTFTY + EL I L Sbjct: 65 VELQNGCICCTLREDLIIEVEKLARLGNIDYILIESTGISEPIPVAQTFTYLDEELGIHL 124 Query: 124 TALARLDTMVTVVDANRFWHDFGSGDSLVDRGTTAGEGDSRDIVDLLIDQIETCDVLLLN 183 LDTMVTVVDANRFW D+ SG+SL+DR T E D+R++VDLLIDQIE +V+LLN Sbjct: 125 GDRCTLDTMVTVVDANRFWEDYESGESLLDRKQTDDEADTREVVDLLIDQIEFANVILLN 184 Query: 184 KCDLVEEQELNKLEAVLRKLQPSAKIIRTVKGIVDPKEVLNTGRFDFERTSMSSGWIAEL 243 K DLVEE ++ +L AVL KL P A+I+ G VD +L+TG FDF++ S +GWI EL Sbjct: 185 KVDLVEEDDVRELTAVLHKLNPEAEILPVSHGRVDLNRILHTGLFDFDKASQGAGWIKEL 244 Query: 244 NKEEHTPETEEYGISSFVYRRKVPFHPQRLSFFFSNWPEEVVRAKGLVWLAAKGDLAASL 303 N EEHTPETEEYGISSFVY+R+ PFHP+R + NWP+EVVRAKG WLA + ++A + Sbjct: 245 N-EEHTPETEEYGISSFVYKRQRPFHPERWMEWLENWPQEVVRAKGFFWLATRNEMAGLI 303 Query: 304 SQAGPSIQFGPAGYWLASLPEEQQLEVLATEPDMKARWDETWGDRMNEVVFIGVGINRAG 363 SQAG SI AG W+A+ +E+Q +VLA EP++ ARW +GDRMNE+VFIG+ ++R Sbjct: 304 SQAGTSIVIQGAGLWVAAYDKEEQQQVLAEEPELAARWHRKYGDRMNELVFIGLDMDRKE 363 Query: 364 IEARLDRCLLNEEEMQQDWSRFNNPLP 390 IE LD CLL +EE+ QDWS +PLP Sbjct: 364 IEKSLDTCLLTDEELGQDWSALPDPLP 390 >SA0410 [R] COG0523 Putative GTPases (G3E family) Length = 400 Score = 481 bits (1239), Expect = e-136, Method: Compositional matrix adjust. Identities = 236/394 (59%), Positives = 284/394 (72%), Gaps = 3/394 (0%) Query: 1 MKPIPVTVLSGYLGSGKTTLLNHILHNRDGLKVAVIVNDMSEVNVDAGLVKSGNTLSRTE 60 M IPVTVLSGYLGSGKTTLLNHIL NR+G ++AVIVNDMSEVN+D LV G LSRT+ Sbjct: 1 MAKIPVTVLSGYLGSGKTTLLNHILQNREGRRIAVIVNDMSEVNIDKDLVADGGGLSRTD 60 Query: 61 EKLVEMSNGCICCTLRDDLLREVHTLAAEGRFDYILIESSGISEPVPVAQTFTYANPELD 120 EKLVE+SNGCICCTLRDDLL+EV L +G D I+IES+GISEPVPVAQTF+Y + EL Sbjct: 61 EKLVELSNGCICCTLRDDLLKEVERLVKKGGIDQIVIESTGISEPVPVAQTFSYIDDELG 120 Query: 121 IDLTALARLDTMVTVVDANRFWHDFGSGDSLVDRGTTAGEGDSRDIVDLLIDQIETCDVL 180 IDLTA+ RLDTMVTVVDANRF HD S D L+DR + E D R I DLLIDQ+E CDVL Sbjct: 121 IDLTAICRLDTMVTVVDANRFVHDINSEDLLMDRDQSVDETDERSIADLLIDQVEFCDVL 180 Query: 181 LLNKCDLVEEQELNKLEAVLRKLQPSAKIIRTVKGIVDPKEVLNTGRFDFERTSMSSGWI 240 ++NK DL+ E+EL KLE VL LQP AKII+T VD KEVLNT RFDFE+ S S+GWI Sbjct: 181 IINKIDLISEEELAKLEKVLSALQPIAKIIKTTNSEVDLKEVLNTQRFDFEKASESAGWI 240 Query: 241 AEL---NKEEHTPETEEYGISSFVYRRKVPFHPQRLSFFFSNWPEEVVRAKGLVWLAAKG 297 EL HTPETEEYGISSFVY+R++PFH +R + + + VVR+KG+VWLA Sbjct: 241 KELESGGHASHTPETEEYGISSFVYKRRLPFHAKRFNDWLESMSNNVVRSKGIVWLAQYN 300 Query: 298 DLAASLSQAGPSIQFGPAGYWLASLPEEQQLEVLATEPDMKARWDETWGDRMNEVVFIGV 357 +A LSQAG S P YW+AS+ E QQ ++LA D+ A WD +GDR + V IG Sbjct: 301 HVACLLSQAGSSCNIHPVTYWVASMSEAQQTQILAERQDVAAEWDPEYGDRHTQFVIIGT 360 Query: 358 GINRAGIEARLDRCLLNEEEMQQDWSRFNNPLPW 391 ++ + LD CL+N +E+ DW +F +P W Sbjct: 361 ELDEEKLTKELDACLVNAQEIDADWQQFEDPYQW 394 >AGl3257 [R] COG0523 Putative GTPases (G3E family) Length = 190 Score = 385 bits (988), Expect = e-107, Method: Compositional matrix adjust. Identities = 0/394 (0%), Positives = 0/394 (0%), Gaps = 7/394 (1%) Query: 1 MKPIPVTVLSGYLGSGKTTLLNHILHNRDGLKVAVIVNDMSEVNVDAGLVKSGNT-LSRT 59 + ++ + + A K + Sbjct: 32 IAKAAAISRPALYLIFPDKEAIFTRVIEMMDARSLDLIQNEVDQIAAIDQKLLHACTIWG 91 Query: 60 EEKLVEMSNGCICCTLRDDLLREVHTLAAEGRFDYILIESSGISEPVPVAQTFTYANPEL 119 + + L D V + + + I G + +A L Sbjct: 92 LHGVELATAYPDAADLFDFQFPAVRQVYERFQQFLVRILEKGTASWELPVSRADFAT-TL 150 Query: 120 DIDLTALARLDTMVTVVDANRFWHDFGSGDSLVDRGTTAGEGDSRDIVDLLIDQIETCDV 179 Sbjct: 151 210 Query: 180 LLLNKCDLVEEQELNKLEAVLRKLQPSAKIIRTVKGIVDPKEVLNTGRFDFERTSMSSGW 239 Sbjct: 211 270 Query: 240 IAELNK-EEHTPETEEYGISSFVYRRKVPFHPQRLSFFFSN-WPEEVVRAKGLVWLAAKG 297 Sbjct: 271 - 329 Query: 298 DLAASLSQAGPSIQFGPAGYWLASLPEEQQLEVLATEPDMKARWDETWGDRMNEVVFIGV 357 Sbjct: 330 389 Query: 358 G-INRAGIEARLDRCLLNEEEMQQD-WSRFNNPL 389 Sbjct: 390 423 >PA5535 [R] COG0523 Putative GTPases (G3E family) Length = 400 Score = 375 bits (964), Expect = e-104, Method: Compositional matrix adjust. Identities = 198/391 (50%), Positives = 265/391 (67%), Gaps = 7/391 (1%) Query: 4 IPVTVLSGYLGSGKTTLLNHILHNRDGLKVAVIVNDMSEVNVDAGLVKSGNTLSRTEEKL 63 +PVTVLSG+LG+GK+TLLNH+L NR+ +VAVIVNDMSE+N+D V+ +L+R EE+L Sbjct: 5 LPVTVLSGFLGAGKSTLLNHVLKNRENRRVAVIVNDMSEINIDGSEVQRDVSLNRAEERL 64 Query: 64 VEMSNGCICCTLRDDLLREVHTLAAEGRFDYILIESSGISEPVPVAQTFTYANPELDIDL 123 VEMSNGCICCTLR+DLL EV LA EGRFDY+LIES+GISEP+PVA+TFT+ + ELD L Sbjct: 65 VEMSNGCICCTLREDLLEEVGRLAREGRFDYLLIESTGISEPMPVAETFTFRD-ELDRSL 123 Query: 124 TALARLDTMVTVVDANRFWHDFGSGDSLVDRGTTAGEGDSRDIVDLLIDQIETCDVLLLN 183 + +ARLDTMVTVVD F D+ SL RG T GE D R I DLLIDQ+E DV+L++ Sbjct: 124 SDVARLDTMVTVVDGMNFLRDYQEAASLASRGETLGEEDQRSITDLLIDQVEFADVILVS 183 Query: 184 KCDLVEEQELNKLEAVLRKLQPSAKIIRTVKGIVDPKEVLNTGRFDFERTSMSSGWIAEL 243 K DL+ ++ +L A+LR+L A+I+ V G V ++L+TGRFDF+R + + GW+ EL Sbjct: 184 KIDLISSEQREELLAILRQLNAEAEILPMVMGQVPLAKILDTGRFDFDRAASAPGWLKEL 243 Query: 244 NKEEHTPETEEYGISSFVYRRKVPFHPQRLSFFFSNWPEE--VVRAKGLVWLAAKGDLAA 301 + EH PETEEYGI+S YR + PFHP R F N ++ ++R+KG WLA++ A Sbjct: 244 -RGEHLPETEEYGIASMAYRARRPFHPARFHAFLHNPLKQGRLLRSKGFFWLASRPREAG 302 Query: 302 SLSQAGPSIQFGPAGYWLASLPEEQQLEVLATEPDMKARWDETWGDRMNEVVFIGVGINR 361 S SQAG +++G AG W +P Q + + W+E GD E+VFIG ++ Sbjct: 303 SWSQAGGLMRYGLAGRWWRFVPTGQWPQDEEGLRAIMRHWEEGVGDCRQELVFIGQNLDF 362 Query: 362 AGIEARLDRCLLNEEEMQ---QDWSRFNNPL 389 A + A LD CLL++ EM W ++P Sbjct: 363 AALRAALDACLLDDAEMNLGIPGWQGLDDPF 393 >BMEII0179 [R] COG0523 Putative GTPases (G3E family) Length = 400 Score = 372 bits (956), Expect = e-103, Method: Compositional matrix adjust. Identities = 201/394 (51%), Positives = 260/394 (65%), Gaps = 7/394 (1%) Query: 2 KPIPVTVLSGYLGSGKTTLLNHILHNRDGLKVAVIVNDMSEVNVDAGLVKSGNT-LSRTE 60 K +PVTVLSG+LG+GKTTLLNH+L+NR+ +VAVIVNDMSE+NVDA L++ G LSRTE Sbjct: 3 KRLPVTVLSGFLGAGKTTLLNHVLNNRENRRVAVIVNDMSEINVDAALIREGGADLSRTE 62 Query: 61 EKLVEMSNGCICCTLRDDLLREVHTLAAEGRFDYILIESSGISEPVPVAQTFTYANPELD 120 E+LVEM+NGCICCTLRDDLL+EV LAA+GRFDY+LIES+GISEP+PVA TF + + E Sbjct: 63 EQLVEMTNGCICCTLRDDLLKEVSQLAAQGRFDYLLIESTGISEPLPVAATFEFRD-ERG 121 Query: 121 IDLTALARLDTMVTVVDANRFWHDFGSGDSLVDRGTTAGEGDSRDIVDLLIDQIETCDVL 180 L+ ARLDTMVTVVDA F D+ SGD L DRG + GE D R +VDLL++QIE +V+ Sbjct: 122 KSLSDGARLDTMVTVVDAANFLKDYASGDFLRDRGESLGEEDERTLVDLLVEQIEFANVV 181 Query: 181 LLNKCDLVEEQELNKLEAVLRKLQPSAKIIRTVKGIVDPKEVLNTGRFDFERTSMSSGWI 240 +LNK +V ++E V+R L P A+I T G V +L+T FDF + W Sbjct: 182 VLNKISMVSKEECALARKVIRSLNPDARIEETDFGQVPLNTILDTRLFDFNKAHEHPLWY 241 Query: 241 AELNK-EEHTPETEEYGISSFVYRRKVPFHPQRLSFFF-SNWPEEVVRAKGLVWLAAKGD 298 EL +H PETEEYG SFVYR + PF P R F NWP VVR+KG WLA + D Sbjct: 242 KELYGFNQHVPETEEYGARSFVYRARKPFDPARFQAFIDQNWP-GVVRSKGFFWLATRPD 300 Query: 299 LAASLSQAGPSIQFGPAGYWLASLPEEQQLEVLATEPDMKARWDETWGDRMNEVVFIGVG 358 +SQAG ++ G W +++P+ + M+ + E WGDR E+VFIG+ Sbjct: 301 FVGEISQAGALVRTSKRGRWWSAVPKHYWPAEPEWQRAMQPYFHEVWGDRRQEIVFIGID 360 Query: 359 -INRAGIEARLDRCLLNEEEMQQD-WSRFNNPLP 390 + + I A LD+CL+ EE + WS ++P P Sbjct: 361 PMRQEAIIAELDKCLVQEECFAPECWSGLSDPFP 394 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.317 0.135 0.402 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 31,011,343 Number of extensions: 1393557 Number of successful extensions: 4856 Number of sequences better than 1.0e-05: 54 Number of HSP's gapped: 4711 Number of HSP's successfully gapped: 54 Length of query: 405 Length of database: 38,378,431 Length adjustment: 109 Effective length of query: 296 Effective length of database: 25,807,025 Effective search space: 7638879400 Effective search space used: 7638879400 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 117 (49.7 bits)