BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0291.0021.p (255 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value lin0193 [P] COG1108 ABC-type Mn2+/Zn2+ transport systems, permea... 198 6e-51 BS_yceA [P] COG1108 ABC-type Mn2+/Zn2+ transport systems, permea... 187 1e-47 L165709 [P] COG1108 ABC-type Mn2+/Zn2+ transport systems, permea... 183 2e-46 SPy0094 [P] COG1108 ABC-type Mn2+/Zn2+ transport systems, permea... 175 5e-44 lin1484 [P] COG1108 ABC-type Mn2+/Zn2+ transport systems, permea... 162 5e-40 >lin0193 [P] COG1108 ABC-type Mn2+/Zn2+ transport systems, permease components Length = 268 Score = 198 bits (504), Expect = 6e-51, Method: Compositional matrix adjust. Identities = 108/220 (49%), Positives = 153/220 (69%), Gaps = 1/220 (0%) Query: 1 VYLMLRRQVLMADTLSHVSLAGVALGSVLQLSPALSGFAVAIAGGLVIEQLRRSYRTYSE 60 V+L++RRQ LMADTLSHVSLAGVA G VL ++P+++ V + L IE LR Y TYSE Sbjct: 28 VFLIIRRQSLMADTLSHVSLAGVAFGLVLNVNPSVTTLIVVVIAALGIEYLRGVYATYSE 87 Query: 61 LPVAIIMTSGLALAVVLMSLKQN-LSRSFSSYLFGSIVAVSDTQLGLIAIVAAVGLSYFI 119 L +AI+M GLA+A+VLMSL Q ++ S YLFGSIV +S Q+ L+ I+ + F+ Sbjct: 88 LSIAILMAGGLAVALVLMSLDQGGITTSVQQYLFGSIVTISKAQVQLLVILGIAIVVLFL 147 Query: 120 LLRRPLYSLTFDEETASIGGVRTGLLSFSFAVLTGMTVAAAMPVVGVLLVSALMVLPASI 179 + +R ++ LTF E+ A GV L+S F+V+TG+ +A MP+ G LLVSAL++LPA+I Sbjct: 148 VFKRFMFVLTFSEDVAVAEGVPVRLISLLFSVVTGIAIAVIMPIAGALLVSALIILPAAI 207 Query: 180 ALRVASGFSAAILISIGVGLTGVFSGLTASYYINTPPGGT 219 +R++ GF ++ +I +GL G+FSGL +SY + TPPG T Sbjct: 208 GMRISKGFLMCVVSAILIGLIGMFSGLVSSYQLGTPPGAT 247 >BS_yceA [P] COG1108 ABC-type Mn2+/Zn2+ transport systems, permease components Length = 350 Score = 187 bits (476), Expect = 1e-47, Method: Compositional matrix adjust. Identities = 18/250 (7%), Positives = 42/250 (16%), Gaps = 1/250 (0%) Query: 1 VYLMLRRQVLMADTLSHVSLAGVALGSVLQLSPALSGFAVAIAGGLVIEQLRRSYRTYSE 60 + T + AL ++ A G I+ + R+ + Sbjct: 28 SFYKYFHIADPKATRDALYQLFTALNVFGRVYLAHEGINAQISVPASNVETFRAQLYAFD 87 Query: 61 LPVAIIMTSGLALAVVLMSL-KQNLSRSFSSYLFGSIVAVSDTQLGLIAIVAAVGLSYFI 119 + + + + R + +G A V Sbjct: 88 PALEGLRLNIALDDDGKSFWVLRMKVRDRIVADGIDDPHFDASNVGEYLQAAEVNAMLDD 147 Query: 120 LLRRPLYSLTFDEETASIGGVRTGLLSFSFAVLTGMTVAAAMPVVGVLLVSALMVLPASI 179 + E + + +F V +V Sbjct: 148 PDALFIDMRNHYEYEVGHFENALEIPADTFREQLPKAVEMMQAHKDKKIVMYCTGGIRCE 207 Query: 180 ALRVASGFSAAILISIGVGLTGVFSGLTASYYINTPPGGTXXXXXXXXXXXXXXVQKLLA 239 + + G ++ + G Sbjct: 208 KASAWMKHNGFNKVWHIEGGIIEYARKAREQGLPVRFIGKNFVFDERMGERISDEIIAHC 267 Query: 240 FQKRRSVRKS 249 Q Sbjct: 268 HQCGAPCDSH 277 >L165709 [P] COG1108 ABC-type Mn2+/Zn2+ transport systems, permease components Length = 269 Score = 183 bits (464), Expect = 2e-46, Method: Compositional matrix adjust. Identities = 101/216 (46%), Positives = 145/216 (67%), Gaps = 1/216 (0%) Query: 1 VYLMLRRQVLMADTLSHVSLAGVALGSVLQLSPALSGFAVAIAGGLVIEQLRRSYRTYSE 60 V+L+LRRQ LM+DTLSHVSLAGVA G +L +P ++ + + +E LR Y Y E Sbjct: 29 VFLVLRRQSLMSDTLSHVSLAGVAFGVLLSWNPTITTLITVVIAAVFLEYLRTIYHNYME 88 Query: 61 LPVAIIMTSGLALAV-VLMSLKQNLSRSFSSYLFGSIVAVSDTQLGLIAIVAAVGLSYFI 119 + AI+M++GLA+A+ +L S K S S YLFGSI+ +S +Q+ ++ ++AAV L FI Sbjct: 89 IATAILMSAGLAIALLILNSSKGATSVSLEQYLFGSIITISLSQVIMLFVLAAVVLLGFI 148 Query: 120 LLRRPLYSLTFDEETASIGGVRTGLLSFSFAVLTGMTVAAAMPVVGVLLVSALMVLPASI 179 L RPLY +TFDE+TA + G+ +S +F ++TG+ +A +P G LLVSA+MVLPASI Sbjct: 149 LFLRPLYVMTFDEDTAFVDGLPVRWISIAFNIVTGVAIALMIPAAGALLVSAIMVLPASI 208 Query: 180 ALRVASGFSAAILISIGVGLTGVFSGLTASYYINTP 215 A+R+ F A + IS V G+ +GL ASYY++ P Sbjct: 209 AMRLGKSFKAVLFISAIVSFIGLNAGLIASYYMDAP 244 >SPy0094 [P] COG1108 ABC-type Mn2+/Zn2+ transport systems, permease components Length = 271 Score = 175 bits (444), Expect = 5e-44, Method: Compositional matrix adjust. Identities = 98/220 (44%), Positives = 144/220 (65%), Gaps = 1/220 (0%) Query: 1 VYLMLRRQVLMADTLSHVSLAGVALGSVLQLSPALSGFAVAIAGGLVIEQLRRSYRTYSE 60 ++L+LRRQ LM+DTLSHVSLAGVALG VL +SP ++ V + +++E LR Y+ Y E Sbjct: 29 IFLILRRQSLMSDTLSHVSLAGVALGVVLGISPTITTIIVVVLAAILLEYLRVVYKHYME 88 Query: 61 LPVAIIMTSGLALA-VVLMSLKQNLSRSFSSYLFGSIVAVSDTQLGLIAIVAAVGLSYFI 119 + AI+M+ GLAL+ +++ + S S YLFGSI+ +S Q+ + +AA+ L + Sbjct: 89 ISTAILMSLGLALSLIIMSKSHSSSSMSLEQYLFGSIITISMEQVVALFAIAAIILILTV 148 Query: 120 LLRRPLYSLTFDEETASIGGVRTGLLSFSFAVLTGMTVAAAMPVVGVLLVSALMVLPASI 179 L RP+Y LTFDE+TA + G+ L+S F ++TG+ +A +P G LLVS +MVLPASI Sbjct: 149 LFIRPMYILTFDEDTAFVDGLPVRLMSVLFNIVTGVAIALTIPAAGALLVSTIMVLPASI 208 Query: 180 ALRVASGFSAAILISIGVGLTGVFSGLTASYYINTPPGGT 219 A+R+ F IL+ I +G +G+ SG+ SY+ TP T Sbjct: 209 AMRLGKNFKTVILLGIVIGFSGMLSGIFLSYFFETPASAT 248 >lin1484 [P] COG1108 ABC-type Mn2+/Zn2+ transport systems, permease components Length = 292 Score = 162 bits (409), Expect = 5e-40, Method: Compositional matrix adjust. Identities = 87/225 (38%), Positives = 136/225 (60%), Gaps = 8/225 (3%) Query: 2 YLMLRRQVLMADTLSHVSLAGVALGSVLQ--------LSPALSGFAVAIAGGLVIEQLRR 53 ++++R+ LMAD LSHV+L G+A+ L L+P GF ++ G L++E+LR Sbjct: 31 FIVVRKLSLMADALSHVTLGGIAVSLFLSKTYLPLAALNPLYLGFGFSVVGSLLMEKLRT 90 Query: 54 SYRTYSELPVAIIMTSGLALAVVLMSLKQNLSRSFSSYLFGSIVAVSDTQLGLIAIVAAV 113 Y+ + EL + IIM++G+ +V+ +SL + SYLFGS+ AVS T + I + A + Sbjct: 91 VYKHFEELAIPIIMSAGMGFSVIFISLANGFNTDLFSYLFGSVSAVSRTDMITIVVTAII 150 Query: 114 GLSYFILLRRPLYSLTFDEETASIGGVRTGLLSFSFAVLTGMTVAAAMPVVGVLLVSALM 173 + L + L+ L+FDEE A + G++ + F VL + +A++M +VG+LLVS+LM Sbjct: 151 VFIVILSLYKELFLLSFDEEYAKVSGLKAKIFDIIFMVLVALVIASSMRIVGILLVSSLM 210 Query: 174 VLPASIALRVASGFSAAILISIGVGLTGVFSGLTASYYINTPPGG 218 LP + A+R+A GF IL+SI G V GL +YY+N PGG Sbjct: 211 TLPVAAAIRIAKGFKQTILLSILFGEIAVIGGLFTAYYLNLAPGG 255 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.324 0.137 0.366 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 15,259,081 Number of extensions: 570933 Number of successful extensions: 2847 Number of sequences better than 1.0e-05: 54 Number of HSP's gapped: 2805 Number of HSP's successfully gapped: 54 Length of query: 255 Length of database: 38,378,431 Length adjustment: 104 Effective length of query: 151 Effective length of database: 26,383,695 Effective search space: 3983937945 Effective search space used: 3983937945 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.0 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.6 bits) S2: 114 (48.5 bits)