BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0291.0020.p (368 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value lin0191 [P] COG0803 ABC-type metal ion transport system, peripla... 240 2e-63 SPy0714_1 [P] COG0803 ABC-type metal ion transport system, perip... 206 5e-53 BS_ycdH [P] COG0803 ABC-type metal ion transport system, peripla... 186 6e-47 SP1002 [P] COG0803 ABC-type metal ion transport system, periplas... 158 1e-38 SP2169_1 [P] COG0803 ABC-type metal ion transport system, peripl... 155 1e-37 >lin0191 [P] COG0803 ABC-type metal ion transport system, periplasmic component/surface adhesin Length = 312 Score = 240 bits (613), Expect = 2e-63, Method: Compositional matrix adjust. Identities = 123/312 (39%), Positives = 177/312 (56%), Gaps = 31/312 (9%) Query: 55 DKLDIKVSFYPMYEFTKNIVGDLADVETLIPAGIEPHDWEPTAQDMAEISAADVLVYNGA 114 DKL + +FYPMY+FTKN+ GD A ++ LI AG EPHD+EP+A+D+A+I AADV +YN Sbjct: 31 DKLKVVTTFYPMYDFTKNVAGDNASIDMLIDAGTEPHDYEPSAKDIAKIEAADVFIYNSE 90 Query: 115 GMEGWAQQVIDSAKGTQLVAVEASKGLDIMEGVXXXXXXXXXXXXXXXXXXXXXXXXXXX 174 ME W V+ S +L ++ASKG+ ++EG Sbjct: 91 DMETWVPSVLKSLDSKKLTIIDASKGIKLLEG---------------------------- 122 Query: 175 XXXXGTAEEXXXXXXXXXXXXXXXXVWLAPSLAIKEVRTIEXXXXXXXXXXXXXFQTNSD 234 E VWL+P LA KEV+ I+ ++ N++ Sbjct: 123 ---SEEEEHDHDHGEEGHHHEHDPHVWLSPVLAEKEVKNIQSGLSKADKANADTYKKNAE 179 Query: 235 AYIAKLEQLDQDFKAGLKDTKRKDFITQHAAFGYLAKEYGLTQVPIAGLSPEQEPSAAQM 294 Y KL+ LD FK + K++DF+TQHAAF YLA EY L QV IAGLSP+QEPS A++ Sbjct: 180 KYTDKLKTLDNKFKTAFEGAKQRDFVTQHAAFQYLANEYDLHQVAIAGLSPDQEPSPARL 239 Query: 295 AKIVEFAKAHKVKTIFFETLVSSSVADTIAGEIGAKSAVLNPIEGLTEADRSSNLDYLGI 354 A++ ++ K + + TI+FE + S VA+ +A E GAK VL+PIEG+T+ ++ +DY+ Sbjct: 240 AELQKYVKENNISTIYFEEVASPKVAEALANETGAKLEVLSPIEGITDKEQKKGMDYIAY 299 Query: 355 MRQNLEALKTAL 366 M QNL+AL+ + Sbjct: 300 MEQNLQALQKTI 311 >SPy0714_1 [P] COG0803 ABC-type metal ion transport system, periplasmic component/surface adhesin Length = 322 Score = 206 bits (524), Expect = 5e-53, Method: Compositional matrix adjust. Identities = 109/308 (35%), Positives = 162/308 (52%), Gaps = 22/308 (7%) Query: 56 KLDIKVSFYPMYEFTKNIVGDLADVETLIPAGIEPHDWEPTAQDMAEISAADVLVYNGAG 115 K+ + +FYP+YEFTK ++G+ DV L+ AG EPHD+EP+ +D+ +I AD VY Sbjct: 31 KVKVVTTFYPVYEFTKGVIGNDGDVFMLMKAGTEPHDFEPSTKDIKKIQDADAFVYMDDN 90 Query: 116 MEGWAQQVIDSAKGTQLVAVEASKGLDIMEGVXXXXXXXXXXXXXXXXXXXXXXXXXXXX 175 ME W V S ++ V+ + + ++ G Sbjct: 91 METWVSDVKKSLTSKKVTIVKGTGNMLLVAGAGHDHPHEDADKK---------------- 134 Query: 176 XXXGTAEEXXXXXXXXXXXXXXXXVWLAPSLAIKEVRTIEXXXXXXXXXXXXXFQTNSDA 235 E VWL+P +I V I F+ N+ Sbjct: 135 ------HEHNKHSEEGHNHAFDPHVWLSPYRSITVVENIRDSLSKAYPEKAENFKANAAT 188 Query: 236 YIAKLEQLDQDFKAGLKDTKRKDFITQHAAFGYLAKEYGLTQVPIAGLSPEQEPSAAQMA 295 YI KL++LD+D+ A L D K+K F+TQHAAFGY+A +YGL Q+ I G++P+ EPSA ++A Sbjct: 189 YIEKLKELDKDYTAALSDAKQKSFVTQHAAFGYMALDYGLNQISINGVTPDAEPSAKRIA 248 Query: 296 KIVEFAKAHKVKTIFFETLVSSSVADTIAGEIGAKSAVLNPIEGLTEADRSSNLDYLGIM 355 + ++ K + +K I+FE SS VA T+A E G K+AVL+P+EGLTE + + DY +M Sbjct: 249 TLSKYVKKYGIKYIYFEENASSKVAKTLAKEAGVKAAVLSPLEGLTEKEMKAGQDYFTVM 308 Query: 356 RQNLEALK 363 R+NLE L+ Sbjct: 309 RKNLETLR 316 >BS_ycdH [P] COG0803 ABC-type metal ion transport system, periplasmic component/surface adhesin Length = 152 Score = 186 bits (471), Expect = 6e-47, Method: Compositional matrix adjust. Identities = 0/167 (0%), Positives = 0/167 (0%) Query: 200 VWLAPSLAIKEVRTIEXXXXXXXXXXXXXFQTNSDAYIAKLEQLDQDFKAGLKDTKRKDF 259 Sbjct: 149 208 Query: 260 ITQHAAFGYLAKEYGLTQVPIAGLSPEQEPSAAQMAKIVEFAKAHKVKTIFFETLVSSSV 319 Sbjct: 209 268 Query: 320 ADTIAGEIGAKSAVLNPIEGLTEADRSSNLDYLGIMRQNLEALKTAL 366 Sbjct: 269 315 Score = 105 bits (262), Expect = 1e-22, Method: Compositional matrix adjust. Identities = 10/92 (10%), Positives = 21/92 (22%) Query: 53 KTDKLDIKVSFYPMYEFTKNIVGDLADVETLIPAGIEPHDWEPTAQDMAEISAADVLVYN 112 L + ++ N L + + +AA Sbjct: 33 TPPTLLVCLNRGASVWPAFNENRTLCVNTLSAGQEPLSNLFGGKTPMEHRFAAARWQTGV 92 Query: 113 GAGMEGWAQQVIDSAKGTQLVAVEASKGLDIM 144 + V + +Q+V+V L Sbjct: 93 TGCPQLEEALVSFDCRISQVVSVGTHDILFCA 124 >SP1002 [P] COG0803 ABC-type metal ion transport system, periplasmic component/surface adhesin Length = 305 Score = 158 bits (400), Expect = 1e-38, Method: Compositional matrix adjust. Identities = 101/310 (32%), Positives = 149/310 (48%), Gaps = 37/310 (11%) Query: 57 LDIKVSFYPMYEFTKNIVGDLADVETLIPAGIEPHDWEPTAQDMAEISAADVLVYNGAGM 116 + I SFYP+Y K + GDL DV +I + H +EP+A D+A I ADV VY+ + Sbjct: 32 MKIVTSFYPIYAMVKEVSGDLNDVR-MIQSSSGIHSFEPSANDIAAIYDADVFVYHSHTL 90 Query: 117 EGWAQQVIDSAKGTQLVAVEASKGLDIMEGVXXXXXXXXXXXXXXXXXXXXXXXXXXXXX 176 E WA + + K +++ +EAS+G+ +E V Sbjct: 91 ESWAGSLDPNLKKSKVKVLEASEGM-TLERVPGL-------------------------- 123 Query: 177 XXGTAEEXXXXXXXXXXXXXXXXVWLAPSLAIKEVRTIEXXXXXXXXXXXXXFQTNSDAY 236 E+ WL P A +E + I +Q N+ A+ Sbjct: 124 -----EDVEAGDGVDEKTLYDPHTWLDPEKAGEEAQIIADKLSEVDSEHKETYQKNAQAF 178 Query: 237 IAKLEQLDQDFKAGLKDTKRKDFITQHAAFGYLAKEYGLTQVPIAGLSPEQEPSAAQMAK 296 I K ++L + F+ + +K F+TQH AF YLAK +GL Q+ IAG+SPEQEPS Q+ + Sbjct: 179 IKKAQELTKKFQPKFEKATQKTFVTQHTAFSYLAKRFGLNQLGIAGISPEQEPSPRQLTE 238 Query: 297 IVEFAKAHKVKTIFFETLVSSSVADTIAGEIGAKSAVLNPIEGLTEADRSSNLDYLGIMR 356 I EF K +KVKTIF E+ SS VA+T+ G LNP+E + D++ YL + Sbjct: 239 IQEFVKTYKVKTIFTESNASSKVAETLVKSTGVGLKTLNPLESDPQNDKT----YLENLE 294 Query: 357 QNLEALKTAL 366 +N+ L L Sbjct: 295 ENMSILAEEL 304 >SP2169_1 [P] COG0803 ABC-type metal ion transport system, periplasmic component/surface adhesin Length = 310 Score = 155 bits (391), Expect = 1e-37, Method: Compositional matrix adjust. Identities = 76/164 (46%), Positives = 108/164 (65%) Query: 200 VWLAPSLAIKEVRTIEXXXXXXXXXXXXXFQTNSDAYIAKLEQLDQDFKAGLKDTKRKDF 259 VWL+P AIK V I F+ N+ AYI KL+ LD+ + GL K+K F Sbjct: 141 VWLSPVRAIKLVEHIRDSLSADYPDKKETFEKNAAAYIEKLQALDKAYAEGLSQAKQKSF 200 Query: 260 ITQHAAFGYLAKEYGLTQVPIAGLSPEQEPSAAQMAKIVEFAKAHKVKTIFFETLVSSSV 319 +TQHAAF YLA +YGL QV I+GLSP+ EPSAA++A++ E+ K +K+ I+FE S ++ Sbjct: 201 VTQHAAFNYLALDYGLKQVAISGLSPDAEPSAARLAELTEYVKKNKIAYIYFEENASQAL 260 Query: 320 ADTIAGEIGAKSAVLNPIEGLTEADRSSNLDYLGIMRQNLEALK 363 A+T++ E G K+ VLNP+E LTE D + +Y+ +M +NL+ALK Sbjct: 261 ANTLSKEAGVKTDVLNPLESLTEEDTKAGENYISVMEKNLKALK 304 Score = 86.3 bits (212), Expect = 6e-17, Method: Compositional matrix adjust. Identities = 37/83 (44%), Positives = 57/83 (68%) Query: 56 KLDIKVSFYPMYEFTKNIVGDLADVETLIPAGIEPHDWEPTAQDMAEISAADVLVYNGAG 115 KL+I +FYP+YEFTK + GD A+VE LI AG EPH++EP+A+ +A+I AD VY Sbjct: 28 KLNIVTTFYPVYEFTKQVAGDTANVELLIGAGTEPHEYEPSAKAVAKIQDADTFVYENEN 87 Query: 116 MEGWAQQVIDSAKGTQLVAVEAS 138 ME W +++D+ ++ ++A+ Sbjct: 88 METWVPKLLDTLDKKKVKTIKAT 110 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.314 0.131 0.364 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 18,562,323 Number of extensions: 600244 Number of successful extensions: 976 Number of sequences better than 1.0e-05: 35 Number of HSP's gapped: 968 Number of HSP's successfully gapped: 61 Length of query: 368 Length of database: 38,378,431 Length adjustment: 108 Effective length of query: 260 Effective length of database: 25,922,359 Effective search space: 6739813340 Effective search space used: 6739813340 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (22.0 bits) S2: 116 (49.3 bits)