BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0291.0015.p (205 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value CAC2804 [R] COG0491 Zn-dependent hydrolases, including glyoxylases 208 4e-54 BH2193 [R] COG0491 Zn-dependent hydrolases, including glyoxylases 208 5e-54 BS_yflN [R] COG0491 Zn-dependent hydrolases, including glyoxylases 202 3e-52 DR0658 [R] COG0491 Zn-dependent hydrolases, including glyoxylases 166 2e-41 BH1837 [R] COG0491 Zn-dependent hydrolases, including glyoxylases 78 1e-14 >CAC2804 [R] COG0491 Zn-dependent hydrolases, including glyoxylases Length = 273 Score = 208 bits (530), Expect = 4e-54, Method: Compositional matrix adjust. Identities = 100/205 (48%), Positives = 132/205 (64%), Gaps = 8/205 (3%) Query: 1 VLTHGHFDHVGNLKELMEEWQEVPVYAHPLELPYLTGLQDYPPADPAVGGGLMATVSPLY 60 +LTHGHFDHVG++ +L + W VPVY H LE+PY+TG +DYP ADP V G++A +S + Sbjct: 74 ILTHGHFDHVGSVIKLSKLWS-VPVYIHELEMPYVTGKKDYPLADPTVDEGMVAKISQTF 132 Query: 61 PHRGLHLGEGVHPLPEDGSVPGAKGWTWVHTPGHSPGHISLFRPADKVLISGDAVITVKQ 120 PH + + V PLP D S+P W W+HTPGH+ GHISLFR D VLI GDA T KQ Sbjct: 133 PHTSIDISSNVSPLPSDSSIPYMPDWKWIHTPGHTDGHISLFREKDHVLIVGDAFCTTKQ 192 Query: 121 ESAFAVITQHKELHGPPAYFTTDWIEAEKSVRKLALLDPQMVIPGHGLPMAGPELSGXXX 180 ES +VIT H+E+ GPP Y TTDW +A+ S+ L L P + IP HG ++G E Sbjct: 193 ESLLSVITHHEEISGPPKYLTTDWAKAKNSIICLKNLKPLIAIPSHGEELSGEE------ 246 Query: 181 XLSAQFAHLCKTFKELSVPSQGKFV 205 L+ L F E++ P++G+FV Sbjct: 247 -LTKHLELLINNFDEIAKPAEGQFV 270 >BH2193 [R] COG0491 Zn-dependent hydrolases, including glyoxylases Length = 259 Score = 208 bits (529), Expect = 5e-54, Method: Compositional matrix adjust. Identities = 102/205 (49%), Positives = 136/205 (66%), Gaps = 7/205 (3%) Query: 1 VLTHGHFDHVGNLKELMEEWQEVPVYAHPLELPYLTGLQDYPPADPAVGGGLMATVSPLY 60 +LTHGHFDHVG L+ L++ + VPVYAH LELP+LTG + Y DP+V GGLMA ++ LY Sbjct: 60 ILTHGHFDHVGALRPLLQRFPNVPVYAHELELPFLTGQRHYVKPDPSVPGGLMAKLAWLY 119 Query: 61 PHRGLHLGEGVHPLPEDGSVPGAKGWTWVHTPGHSPGHISLFRPADKVLISGDAVITVKQ 120 P+ + PLP+DG++P W W+HTPGH+ GH+SLFRP+D+ LI+GDA TVKQ Sbjct: 120 PYHAYDFSRIICPLPDDGTLPFFTDWRWIHTPGHTEGHVSLFRPSDRFLIAGDAFTTVKQ 179 Query: 121 ESAFAVITQHKELHGPPAYFTTDWIEAEKSVRKLALLDPQMVIPGHGLPMAGPELSGXXX 180 ES +V+ Q KE+HGPPAYFT DW A+ SV L L+P + GHG M G L Sbjct: 180 ESLLSVLAQKKEIHGPPAYFTPDWELAKTSVETLQKLEPAFAVTGHGQMMEGSSLHSGLK 239 Query: 181 XLSAQFAHLCKTFKELSVPSQGKFV 205 L +Q F++++VP G++V Sbjct: 240 RLISQ-------FEQVAVPDHGRYV 257 >BS_yflN [R] COG0491 Zn-dependent hydrolases, including glyoxylases Length = 264 Score = 202 bits (514), Expect = 3e-52, Method: Compositional matrix adjust. Identities = 96/189 (50%), Positives = 130/189 (68%), Gaps = 1/189 (0%) Query: 1 VLTHGHFDHVGNLKELMEEWQEVPVYAHPLELPYLTGLQDYPPADPAVGGGLMATVSPLY 60 +LTHGHFDH+G ++E++E W +VPVY H E+PY+TG +DYPPA P GL+A +SPL+ Sbjct: 77 ILTHGHFDHIGAIEEILEHW-DVPVYIHSREMPYVTGKEDYPPARPDSKSGLVAKLSPLF 135 Query: 61 PHRGLHLGEGVHPLPEDGSVPGAKGWTWVHTPGHSPGHISLFRPADKVLISGDAVITVKQ 120 P + + V LPEDGSVP W W+ TPGH+PGHISLFR +VL++GDAVITV+Q Sbjct: 136 PRHSIDISSHVQALPEDGSVPFLDEWMWIATPGHTPGHISLFRDDGRVLVAGDAVITVEQ 195 Query: 121 ESAFAVITQHKELHGPPAYFTTDWIEAEKSVRKLALLDPQMVIPGHGLPMAGPELSGXXX 180 E V+ Q +ELHGPPAYFT D A +S+ KLA L+P+ ++ GHG+PM G Sbjct: 196 EKMADVLIQKQELHGPPAYFTPDTETAAESILKLAGLEPEALLTGHGIPMTGKNFRSDLT 255 Query: 181 XLSAQFAHL 189 L+ + + + Sbjct: 256 ELANRLSSI 264 >DR0658 [R] COG0491 Zn-dependent hydrolases, including glyoxylases Length = 364 Score = 166 bits (421), Expect = 2e-41, Method: Compositional matrix adjust. Identities = 95/189 (50%), Positives = 116/189 (61%), Gaps = 3/189 (1%) Query: 1 VLTHGHFDHVGNLKELMEEWQEVPVYAHPLELPYLTGLQDYPPADPAVGGGLMATVSPLY 60 VLTHGHFDH+G L L+ EW VPVYAHP ELP+LTG Y DP VGG LM+ SPL+ Sbjct: 127 VLTHGHFDHIGALHALLAEWN-VPVYAHPDELPHLTGEVPYRFPDPTVGG-LMSLTSPLF 184 Query: 61 PHRGLHLGEGVHPLPEDGSVPGAKGWTWVHTPGHSPGHISLFRPADKVLISGDAVITVKQ 120 V LPEDG+VP GW WVHTPGH+ GH+SL+R AD++LI+GDAV+T Q Sbjct: 185 LPGPYDFRPQVRTLPEDGTVPELPGWRWVHTPGHTEGHVSLWREADRLLIAGDAVVTTPQ 244 Query: 121 ESA-FAVITQHKELHGPPAYFTTDWIEAEKSVRKLALLDPQMVIPGHGLPMAGPELSGXX 179 E+ A+ + L PPAY+T +W SVR LA L P +V GHG P+ L Sbjct: 245 ETVPGALASTPTVLRRPPAYYTPNWNATRDSVRTLAALRPALVATGHGHPLTDDSLGDAL 304 Query: 180 XXLSAQFAH 188 L+ F H Sbjct: 305 TRLADDFDH 313 >BH1837 [R] COG0491 Zn-dependent hydrolases, including glyoxylases Length = 236 Score = 77.8 bits (190), Expect = 1e-14, Method: Compositional matrix adjust. Identities = 54/173 (31%), Positives = 77/173 (44%), Gaps = 25/173 (14%) Query: 1 VLTHGHFDHVGNLKELMEEWQEVPVYAHPLELPYLTGLQDYPPADPAVGGGLMATVSPLY 60 +LTHGH DHVG LK++++ Q++PVY H L++ ++ G + +P PL Sbjct: 60 LLTHGHSDHVGGLKKILD-IQDLPVYVHTLDIKHMEGKEPFPGRKKREYLVEPGVAKPL- 117 Query: 61 PHRGLHLGEGVHPLPEDGSVPGAKGWTWVHTPGHSPGHISLFRPADKVLISGDAVITVKQ 120 P+ EDG HTPGHSPGH+ + D+VLI GD Sbjct: 118 ------------PVDEDGEFRCFGNLVPYHTPGHSPGHVCYYHKKDRVLIGGD------- 158 Query: 121 ESAFAVITQHKELHGPPAYFTTDWIEAEKSVRKLALLDPQMVIPGHGLPMAGP 173 ++ +L P FT D EA +S + L P V HG + P Sbjct: 159 ----LFTSKRGKLQQPIKMFTADMNEAVRSGEIVKKLQPSKVSICHGNDIDNP 207 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.318 0.138 0.441 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 17,331,301 Number of extensions: 768282 Number of successful extensions: 1452 Number of sequences better than 1.0e-05: 25 Number of HSP's gapped: 1448 Number of HSP's successfully gapped: 25 Length of query: 205 Length of database: 38,378,431 Length adjustment: 101 Effective length of query: 104 Effective length of database: 26,729,697 Effective search space: 2779888488 Effective search space used: 2779888488 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 113 (48.1 bits)