BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0290.0078.p (435 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value BH1351 [J] COG0621 2-methylthioadenine synthetase 564 e-161 BS_yqeV [J] COG0621 2-methylthioadenine synthetase 539 e-153 SA1405 [J] COG0621 2-methylthioadenine synthetase 536 e-152 CAC1286 [J] COG0621 2-methylthioadenine synthetase 371 e-102 FN1214 [J] COG0621 2-methylthioadenine synthetase 303 4e-82 >BH1351 [J] COG0621 2-methylthioadenine synthetase Length = 346 Score = 564 bits (1454), Expect = e-161, Method: Compositional matrix adjust. Identities = 0/425 (0%), Positives = 0/425 (0%) Query: 1 VNFYDTEAIWQLFKNEGYEQVDFEGPADVYLINTCTVTNTGDKKSRQMIRRAVRRNPEAI 60 V+ + +N + A IN V R + Sbjct: 13 VSTLGLGTMTFGEQNSEADAHAQLDYAVAQGINLIDVAEMYPVPPRPETQGLTETYVGNW 72 Query: 61 VAVTGCYAQTSPGEILDIPGVDLVIGNQDREHIMTHVKNIQESRQPVNAVRNIMKTREFE 120 +A G + + P + G + + + + + + Sbjct: 73 LAKHGSREKLIIASKVSGPSRNNDKGIRPDQALDRKNIREALHDSLKRLQTDYLDLYQVH 132 Query: 121 ELDVPGFADRTRAFMKIQDGCNNFCTFCIIPWSRGLSRSRDPKSIVAQAYQLVEAGYKEF 180 P + + + + V+ Y Sbjct: 133 WPQRPTNCFGKLGYSWTDSAPAVSLLDTLDALAEYQRAGKIRYIGVSNETAFGVMRYLHL 192 Query: 181 VLTGIHTGGYGDDLENYRLADLLWELDQVDGLERVRISSIEASQIDGKLLDVLNRSSKMC 240 L + G L +K Sbjct: 193 ADKHDLPRIVTIQNPYSLLNRSFEVGLAEVSQYEGVELLAYSCLGFGTLTGKYLNGAKPA 252 Query: 241 RHLHIPLQAGHNEVLKAMRRKYTTEEYYAKMELIRQAMPDVGITTDVIVGFPGETEEMFR 300 + + ++ A+ + A + Sbjct: 253 GARNTLFSRFTRYSGEQTQKAVAAYVDIARRHGLDPAQMALAFVRRQPFVASTLLGATTM 312 Query: 301 AGYDFMKAVNYSEMHVFPYSKRTGTPAARMLNQVDEDIKNARVQQLIDLSEEMQLAYARR 360 Sbjct: 313 372 Query: 361 FVGKTVSVIAERSAKNAPGRSIQHGFSDNYLQVFFNGDDSLQGELCQVKITEAGVNECRG 420 Sbjct: 373 432 Query: 421 ELVSV 425 Sbjct: 433 437 >BS_yqeV [J] COG0621 2-methylthioadenine synthetase Length = 211 Score = 539 bits (1388), Expect = e-153, Method: Compositional matrix adjust. Identities = 0/426 (0%), Positives = 0/426 (0%), Gaps = 1/426 (0%) Query: 1 VNFYDTEAIWQLFKNEGYEQVDFEGPADVYLINTCTVTNTGDKKSRQMIRRAVRRNPEAI 60 + + +L + +E + + + + E + Sbjct: 13 LRENNPHPTTELNFSSPFELLIAVLLSAQATDVSVNKATAKLYPVANTPAAMLELGVEGV 72 Query: 61 VAVTGCYAQTSPGEILDIPGVDLVIGNQDREHIMTHVKNIQESRQPVNAVRNIMKTREFE 120 + I +++ + E ++ T Sbjct: 73 KTYIKTIGLYNSKAENIIKTCRILLEQHNGEVPEDRAALEALPGVGRKTANVVLNTAFGW 132 Query: 121 ELDVPGFADRTRAFMKIQDGCNNFCTFCIIPWSRGLSRSRDPKSIVAQAYQLVEAGYKEF 180 N + + + ++ Sbjct: 133 PTIAVDTHIFRVCNRTQFAPGKNVEQVEEKLLKVVPAEFKVDCHHWLILHGRYTCIARKP 192 Query: 181 VLTGIHTGGYGDDLENYRLADLLWELD-QVDGLERVRISSIEASQIDGKLLDVLNRSSKM 239 Sbjct: 193 252 Query: 240 CRHLHIPLQAGHNEVLKAMRRKYTTEEYYAKMELIRQAMPDVGITTDVIVGFPGETEEMF 299 Sbjct: 253 312 Query: 300 RAGYDFMKAVNYSEMHVFPYSKRTGTPAARMLNQVDEDIKNARVQQLIDLSEEMQLAYAR 359 Sbjct: 313 372 Query: 360 RFVGKTVSVIAERSAKNAPGRSIQHGFSDNYLQVFFNGDDSLQGELCQVKITEAGVNECR 419 Sbjct: 373 432 Query: 420 GELVSV 425 Sbjct: 433 438 >SA1405 [J] COG0621 2-methylthioadenine synthetase Length = 346 Score = 536 bits (1381), Expect = e-152, Method: Compositional matrix adjust. Identities = 0/425 (0%), Positives = 0/425 (0%), Gaps = 3/425 (0%) Query: 1 VNFYDTEAIWQLFKNEGYEQVDFEGPADVYLINTCTVTNTGDKKSRQMIRRAVRRNPEAI 60 V+ + +N + A IN V R + Sbjct: 13 VSTLGLGTMTFGEQNSEADAHAQLDYAVAQGINLIDVAEMYPVPPRPETQGLTETYVGNW 72 Query: 61 VAVTGCYAQTSPGEILDIPGVDLVIGNQDREHIMTHVKNIQESRQPVNAVRNIMKTREFE 120 +A G + + P + G + + + + + + Sbjct: 73 LAKHGSREKLIIASKVSGPSRNNDKGIRPDQALDRKNIREALHDSLKRLQTDYLDLYQVH 132 Query: 121 ELDVPGFADRTRAFMKIQDGCNNFCTFCIIPWSRGLSRSRDPKSIVAQAYQLVEAGYKEF 180 P + + + + V+ Y Sbjct: 133 WPQRPTNCFGKLGYSWTDSAPAVSLLDTLDALAEYQRAGKIRYIGVSNETAFGVMRYLHL 192 Query: 181 VLTGIHTGGYGDDLENYRLADLLWELDQVDGLERVRISSIEASQIDGKLLDVLNRSSKMC 240 L + G L +K Sbjct: 193 ADKHDLPRIVTIQNPYSLLNRSFEVGLAEVSQYEGVELLAYSCLGFGTLTGKYLNGAKPA 252 Query: 241 RHLHIPLQAGHNEVLKAMRRKYTTEEYYAKMELIRQAMPDVGITTDVIVGFPGETEEMFR 300 + + ++ A+ + A + Sbjct: 253 GARNTLFSRFTRYSGEQTQKAVAAYVDIARRHGLDPAQMALAFVRRQPFVASTLLGATTM 312 Query: 301 AGYDFMKAVNYSEMHVFPYSKRTGTPAARMLNQVDEDIKNARVQQLIDLSEEMQLAYARR 360 Sbjct: 313 372 Query: 361 FVGKTVSVIAERSAKNAPGRSIQHGFSDNYLQVFFNGDDSLQGELCQVKITEAGVNECRG 420 Sbjct: 373 --- 429 Query: 421 ELVSV 425 Sbjct: 430 434 >CAC1286 [J] COG0621 2-methylthioadenine synthetase Length = 346 Score = 371 bits (953), Expect = e-102, Method: Compositional matrix adjust. Identities = 0/424 (0%), Positives = 0/424 (0%), Gaps = 3/424 (0%) Query: 1 VNFYDTEAIWQLFKNEGYEQVDFEGPADVYLINTCTVTNTGDKKSRQMIRRAVRRNPEAI 60 + + V + + + Sbjct: 12 EVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGINLIDVAEMYPVPPRPETQGLTETYVGN 71 Query: 61 VAVTGCYAQTSPGEILDIPGVDLVIGNQDREHIMTHVKNIQESRQPVNAVRNIMKTREFE 120 + + + + + ++ Sbjct: 72 WLAKHGSREKLIIASKVSGPSRNNDKGIRPDQALDRKNIREALHDSLKRLQTDYLDLYQV 131 Query: 121 ELDVPGFADRTRAFMKIQDGCNNFCTFCIIPWSRGLSRSRDPKSIVAQAYQLVEAGYKEF 180 + D + R+ + I Sbjct: 132 HWPQRPTNCFGKLGYSWTDSAPAVSLLDTLDALAEYQRAGKIRYIGVSNETAFGVMRYLH 191 Query: 181 VLTGIHTGGYGDDLE-NYRLADLLWELDQVDGLERVRISSIEASQIDGKLLDVLNRSSKM 239 + L + G L +K Sbjct: 192 LADKHDLPRIVTIQNPYSLLNRSFEVGLAEVSQYEGVELLAYSCLGFGTLTGKYLNGAKP 251 Query: 240 CRHLHIPLQAGHNEVLKAMRRKYTTEEYYAKMELIRQAMPDVGITTDVIVGFPGETEEMF 299 + + ++ A+ + A + Sbjct: 252 AGARNTLFSRFTRYSGEQTQKAVAAYVDIARRHGLDPAQMALAFVRRQPFVASTLLGATT 311 Query: 300 RAGYDFMKAVNYSEMHVFPYSKRTGTPAARMLNQVDEDIKNARVQQLIDLSEEMQLAYAR 359 Sbjct: 312 371 Query: 360 RFVGKTVSVIAERSAKNAPGRSIQHGFSDNYLQVFFNGDDSLQGELCQVKITEAGVNECR 419 Sbjct: 372 -- 429 Query: 420 GELV 423 Sbjct: 430 433 >FN1214 [J] COG0621 2-methylthioadenine synthetase Length = 346 Score = 303 bits (775), Expect = 4e-82, Method: Compositional matrix adjust. Identities = 0/412 (0%), Positives = 0/412 (0%), Gaps = 8/412 (1%) Query: 1 VNFYDTEAIWQLFKNEGYEQVDFEGPADVYLINTCTVTNTGDKKSRQMIRRAVRRNPEAI 60 + Q + + + Q+D+ + LI+ + + Q + N A Sbjct: 16 LGLGTMTFGEQNSEADAHAQLDYAVAQGINLIDVAEMYPVPPRPETQGLTETYVGNWLAK 75 Query: 61 VAVTGCYAQTSPGEILDIPGVDLVIGNQDREHIMTHVKNIQESRQPVNAVRNIMKTREFE 120 S + +Q + ++ ++ + + Sbjct: 76 HGSREKLIIASKVSGPSRNNDKGIRPDQALDRKNIREALHDSLKRLQTDYLDLYQVHWPQ 135 Query: 121 ELDVPGFADRTRAFMKIQDGCNNFCTFCIIPWSRGLSRSRDPKSIVAQAYQLVEAGYKEF 180 + + R S + + Sbjct: 136 RPTNCFGKLGYSWTDSAPAVSLLDTLDALAEYQRAGKIRYIGVSNETAFGVMRYLHLADK 195 Query: 181 VLTGIHTGGYGDDLENY-RLADLLWELDQVDGLERVRISSIEASQIDGKLLDVLNRSSKM 239 L E+ Q +G+E + S + + GK L+ + + Sbjct: 196 HDLPRIVTIQNPYSLLNRSFEVGLAEVSQYEGVELLAYSCLGFGTLTGKYLNGA-KPAGA 254 Query: 240 CRHLHIPLQAGHNEVLKAMRRKYTTEEYYAKMELIRQAMPDVGITTDVIVGFPGETEEMF 299 L E + Y ++ + A+ V V G T Sbjct: 255 RNTLFSRFTRYSGEQTQKAVAAYVDIARRHGLDPAQMALAFVRRQPFVASTLLGATTMDQ 314 Query: 300 RAGYDFMKAVNYSEMHVFPYSKRTGTPAARMLNQVDEDIKNARVQQLIDLSEEMQLAYAR 359 Sbjct: 315 374 Query: 360 RFVGKTVSVIAERSAKNAPGRSIQHGFSDNYLQVFFNGDDS-LQGELCQVKI 410 Sbjct: 375 ----- 421 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.320 0.136 0.397 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 31,901,640 Number of extensions: 1253019 Number of successful extensions: 2549 Number of sequences better than 1.0e-05: 75 Number of HSP's gapped: 2363 Number of HSP's successfully gapped: 77 Length of query: 435 Length of database: 38,378,431 Length adjustment: 109 Effective length of query: 326 Effective length of database: 25,807,025 Effective search space: 8413090150 Effective search space used: 8413090150 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 117 (49.7 bits)