BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0290.0077.p (259 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value PA0419 [S] COG1385 Uncharacterized protein conserved in bacteria 107 3e-23 aq_1657 [E] COG0509 Glycine cleavage system H protein (lipoate-b... 73 5e-13 aq_1658 [G] COG0235 Ribulose-5-phosphate 4-epimerase and related... 73 5e-13 TP0276 [S] COG1385 Uncharacterized protein conserved in bacteria 69 7e-12 >PA0419 [S] COG1385 Uncharacterized protein conserved in bacteria Length = 240 Score = 107 bits (266), Expect = 3e-23, Method: Compositional matrix adjust. Identities = 68/220 (30%), Positives = 110/220 (50%), Gaps = 13/220 (5%) Query: 23 ARHIAKVMRGKAGDKLIVSDGVSREALVEIAGIEPGEVTVNILELLDMTHEPRIKITVAQ 82 A +I +V+R GD + + DG RE L E+ + V V + E + ++I + Q Sbjct: 24 AHYIGRVLRHATGDAVQLFDGSGREYLGELVEVGKKNVRVELREQFAGQPDSPLRIHLGQ 83 Query: 83 SLPKGDKLETVIQKCTEIGAVAFVPFLSERTIVQYDERKESKRLERWRKICKEAAEQAHR 142 L +G++++ IQK TE+G P +SER V+ + + KRL+ WR++ A EQ R Sbjct: 84 GLSRGERMDWAIQKATELGVAEITPIVSERCEVRLKDERADKRLQHWRQVAISACEQCGR 143 Query: 143 NIVPEVHSPLSWKLLLKSFSGYDAVYFCYEKEEGLQXXXXXXXXXXXXXXXXTGKVMVVV 202 + +P +H PL+ + L+ +++ L+ + ++ +V Sbjct: 144 SSLPLIHPPLALEDWLRG------------RDDDLRLVLHPVAAPLASHAPPS-RLAFLV 190 Query: 203 GPEGGFSPEECRAAEEAGAVSVGLGRRILRCETAGMVAAA 242 GPEGG S E A+ AG S LG R+LR ETA +VA A Sbjct: 191 GPEGGLSDAEVEQAQAAGFHSARLGPRVLRTETAPVVALA 230 >aq_1657 [E] COG0509 Glycine cleavage system H protein (lipoate-binding) Length = 229 Score = 72.8 bits (177), Expect = 5e-13, Method: Compositional matrix adjust. Identities = 60/188 (31%), Positives = 91/188 (48%), Gaps = 17/188 (9%) Query: 59 EVTVNILELLDMTHEPRIKITVAQSLPKGDK-LETVIQKCTEIGAVAFVPFLSERTIVQY 117 E++ I+E L+ P+ IT+ QS+ K ++T++++ TE+G + FVP +SER+ + Sbjct: 55 EISCEIVEELETKLPPK-DITLYQSVTVDLKTMDTIVRQATELGVLTFVPIISERSFQK- 112 Query: 118 DERKESKRLERWRKICKEAAEQAHRNIVPEVHSPLSWKLLLKSFSGYDAVYFCYEKEEGL 177 E K+ E+W++I EA +Q+ R I E+ P+ L+ E EE + Sbjct: 113 -EEAILKKTEKWKRIVIEAMKQSRRPIPMEIKKPVRLSDLIP------------ESEENI 159 Query: 178 QXXXXXXXXXXXXXXXXTGKVMVVVGPEGGFSPEECRAAEEAGAVSVGLGRRILRCETAG 237 VVVGPEGGFS E + E G SV L LR ETA Sbjct: 160 ILDNFYEGVKPKDVNLEAKTYSVVVGPEGGFSKRESQILREKGFKSVLLEPYTLRTETA- 218 Query: 238 MVAAACIL 245 +V+ IL Sbjct: 219 VVSIVSIL 226 >aq_1658 [G] COG0235 Ribulose-5-phosphate 4-epimerase and related epimerases and aldolases Length = 229 Score = 72.8 bits (177), Expect = 5e-13, Method: Compositional matrix adjust. Identities = 60/188 (31%), Positives = 91/188 (48%), Gaps = 17/188 (9%) Query: 59 EVTVNILELLDMTHEPRIKITVAQSLPKGDK-LETVIQKCTEIGAVAFVPFLSERTIVQY 117 E++ I+E L+ P+ IT+ QS+ K ++T++++ TE+G + FVP +SER+ + Sbjct: 55 EISCEIVEELETKLPPK-DITLYQSVTVDLKTMDTIVRQATELGVLTFVPIISERSFQK- 112 Query: 118 DERKESKRLERWRKICKEAAEQAHRNIVPEVHSPLSWKLLLKSFSGYDAVYFCYEKEEGL 177 E K+ E+W++I EA +Q+ R I E+ P+ L+ E EE + Sbjct: 113 -EEAILKKTEKWKRIVIEAMKQSRRPIPMEIKKPVRLSDLIP------------ESEENI 159 Query: 178 QXXXXXXXXXXXXXXXXTGKVMVVVGPEGGFSPEECRAAEEAGAVSVGLGRRILRCETAG 237 VVVGPEGGFS E + E G SV L LR ETA Sbjct: 160 ILDNFYEGVKPKDVNLEAKTYSVVVGPEGGFSKRESQILREKGFKSVLLEPYTLRTETA- 218 Query: 238 MVAAACIL 245 +V+ IL Sbjct: 219 VVSIVSIL 226 >TP0276 [S] COG1385 Uncharacterized protein conserved in bacteria Length = 294 Score = 68.9 bits (167), Expect = 7e-12, Method: Compositional matrix adjust. Identities = 62/268 (23%), Positives = 115/268 (42%), Gaps = 32/268 (11%) Query: 1 MQRYFVTPEQFHPDT---VMISGEDARHIAKVMRGKAGDKLIVSD--GVSREALVEIAGI 55 M++ +T E PD+ V +SG+D ++ +V R + GD + G A+V I Sbjct: 16 MKQLLITAE---PDSGGYVQLSGKDFHYMVRVRRARVGDTFTLRTQAGAQYSAVVSALDI 72 Query: 56 EPGEVTVNILELLDMTHEPRIKITVAQSLPKGDKLETVIQKCTEIGAVAFVPFLSERTIV 115 + ++ + + + ++ + + Q +P+ + +++ TE+G +P ER+++ Sbjct: 73 HTRSLILHCTPMPPVDEKTQVTLVLLQWMPRARVFDAIVRHATELGTARIIPIRGERSLI 132 Query: 116 QYDERKESKRLERWRKICKEAAEQAHRNI---VPEVHSPL-SWKLLLKSFSGYDAV-YFC 170 + ++ + RL+R I +EA +Q+ + V +HS L + +L + F AV +C Sbjct: 133 RDEKDTQHARLQR---IVREARQQSGSAVNTRVDTLHSLLGALHMLEQEFPYPSAVRIYC 189 Query: 171 YEKEEG----------------LQXXXXXXXXXXXXXXXXTGKVMVVVGPEGGFSPEECR 214 EK G V++ VG EGGFS E R Sbjct: 190 SEKHVGGSFHELLWTGRSTHCTAHRTPTEPNAPHNTPDVRAQAVILAVGAEGGFSDTEER 249 Query: 215 AAEEAGAVSVGLGRRILRCETAGMVAAA 242 +A + +LR ETA + A A Sbjct: 250 TLHDAHFAPLHFRTNVLRVETAALYALA 277 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.318 0.135 0.396 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 17,712,782 Number of extensions: 706384 Number of successful extensions: 2315 Number of sequences better than 1.0e-05: 4 Number of HSP's gapped: 2312 Number of HSP's successfully gapped: 4 Length of query: 259 Length of database: 38,378,431 Length adjustment: 104 Effective length of query: 155 Effective length of database: 26,383,695 Effective search space: 4089472725 Effective search space used: 4089472725 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 115 (48.9 bits)