BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0290.0062.p (1353 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value BS_vpr [O] COG1404 Subtilisin-like serine proteases 260 1e-68 BH0831 [O] COG1404 Subtilisin-like serine proteases 226 2e-58 SPy2010 [O] COG1404 Subtilisin-like serine proteases 94 2e-18 BS_aprE [O] COG1404 Subtilisin-like serine proteases 86 3e-16 BS_ispA [O] COG1404 Subtilisin-like serine proteases 79 4e-14 >BS_vpr [O] COG1404 Subtilisin-like serine proteases Length = 806 Score = 260 bits (664), Expect = 1e-68, Method: Compositional matrix adjust. Identities = 242/769 (31%), Positives = 364/769 (47%), Gaps = 129/769 (16%) Query: 40 INTKSSINVRVIVQLSGQPAAVGKYAAKQGISALAKTATEAAVKSQQADVLDQAEAKGID 99 I+ +S VIV+L K+ A AK A E+ KS+ +A+ K I Sbjct: 48 IDMTTSKKTTVIVEL------------KEKSLAEAKEAGESQSKSKLKTARTKAKNKAIK 95 Query: 100 LT----VNYKYDTILNGFEVTVPADKIPELAKISGVTSIYPNSTWYA--LPDQTVTETT- 152 VN +Y+ + +GF + +PA++IP+L + V ++YPN T+ + D+ VT + Sbjct: 96 AVKNGKVNREYEQVFSGFSMKLPANEIPKLLAVKDVKAVYPNVTYKTDNMKDKDVTISED 155 Query: 153 ----YRNDNAPLTQIHADWAEAKGFNGAGLKVGVIDTGVDYKHPDIA---AAYKGGYDSF 205 +D+AP + W G+ G G+KV +IDTGV+Y HPD+ YKG YD Sbjct: 156 AVSPQMDDSAPYIGANDAWD--LGYTGKGIKVAIIDTGVEYNHPDLKKNFGQYKG-YDFV 212 Query: 206 YQDNDPYEEEPLTPAEDPXXXXXXXXXXXXXXXXTIIGQFAANGNTAQKGVAPGADLYVY 265 D DP E TP DP + G AANG KGVAP A L Y Sbjct: 213 DNDYDPKE----TPTGDPRGEATDHGTH-------VAGTVAANGTI--KGVAPDATLLAY 259 Query: 266 KVLGRNLDKPSTSSGSSAQVIDGIEHAVKDGMDVINLSLGSDSEKDVNSPD---SIAINN 322 +VLG SG++ VI G+E AV+DG DV+NLSLG+ +N+PD S A++ Sbjct: 260 RVLGPG------GSGTTENVIAGVERAVQDGADVMNLSLGNS----LNNPDWATSTALDW 309 Query: 323 AVLSGVTAVIANGNNGEAGYFSLGSPAASQLAISVGSVTSPIDAYSGKFKAEIANSVTSV 382 A+ GV AV +NGN+G G+ ++GSP S+ AISVG+ P++ Y+ V Sbjct: 310 AMSEGVVAVTSNGNSGPNGW-TVGSPGTSREAISVGATQLPLNEYA-------------V 355 Query: 383 TYDTYFPFHIMAYELEKSDFASADIIGTDPVRVVYANLGADEDYPEGDLSKTIVLISRGD 442 T+ +Y +M Y K D A + V +V A +G +D+ DL+ + ++ RG Sbjct: 356 TFGSYSSAKVMGYN--KEDDVKA--LNNKEVELVEAGIGEAKDFEGKDLTGKVAVVKRGS 411 Query: 443 LTFQDKIANAKAHGVKALVIFNGNARKVDNKSEAILDESYTDRSGFIGINLGNGYQNVPT 502 + F DK NAK G +V++N + SG I N+ +VPT Sbjct: 412 IAFVDKADNAKKAGAIGMVVYN-------------------NLSGEIEANVPG--MSVPT 450 Query: 503 FDIKGSEGRKLARAIIANGDRPTYFTFGSEYQHEMTTGDTMSTFSSWGPNVDGNLSIKPD 562 + +G KL A+ A G+ T F G+ ++ FSS GP +D IKPD Sbjct: 451 IKLSLEDGEKLVSALKA-GETKTTFKL----TVSKALGEQVADFSSRGPVMD-TWMIKPD 504 Query: 563 IVAPGDGVLSTYPAYGGDYSKAYQRSSGTSMATPHVAGLSLLIKQAHPEYTPFDIRAVLA 622 I APG ++ST P + D+ Y GTSMA+PH+AG +IKQA P+++ I+A + Sbjct: 505 ISAPGVNIVSTIPTHDPDHPYGYGSKQGTSMASPHIAGAVAVIKQAKPKWSVEQIKAAIM 564 Query: 623 NTADPILYNKAPEDLY---IQGPGRANVENAILTPALLEAVEPITILDKNYVAQSVTNYN 679 NTA + + ++Y QG G A + NAI +L V P + ++ + N N Sbjct: 565 NTA--VTLKDSDGEVYPHNAQGAGSARIMNAIKADSL---VSPGSYSYGTFLKE---NGN 616 Query: 680 PSTGFGVLTPGSKSSKTLQLKNLSSSDVAYTASVKWNYGDGKVTAALDKTTLTASAHGTT 739 + ++T ++N SS +YT +N G G T+ + + AH T Sbjct: 617 ET-----------KNETFTIENQSSIRKSYTLEYSFN-GSGISTSGTSRVVI--PAHQTG 662 Query: 740 GFNLNVAVAPDANPEQPYQGNVILTAEGQPTLHLPFTVHVNDKAEQPDW 788 V V Y+G VI+ G+ +P + V ++PD+ Sbjct: 663 KATAKVKVNTKKTKAGTYEGTVIVREGGKTVAKVPTLLIV----KEPDY 707 >BH0831 [O] COG1404 Subtilisin-like serine proteases Length = 799 Score = 226 bits (576), Expect = 2e-58, Method: Compositional matrix adjust. Identities = 238/780 (30%), Positives = 357/780 (45%), Gaps = 145/780 (18%) Query: 39 QINTKSSINVRVIVQLSGQPAAVGKYAAKQGISALAKTATEAAVKSQQADVLDQAEAKGI 98 +I+T SS VIV++ P+ + A QG + ++ +K + V++Q + Sbjct: 42 EIDTSSSALETVIVEIE-DPSII--EAKHQG-----QKQSKNELKQARQSVIEQIQDLVP 93 Query: 99 DLTVNYKYDTILNGFEVTVPADKIPELAKISGVTSIYPNSTWYALP--DQTVTETTYRN- 155 TV ++YD + +GF + +PA +IP + I GV ++YPN + D+ V E + Sbjct: 94 SSTVTHEYDFLFSGFALELPAHQIPSILGIDGVHAVYPNIEYEVETDFDEVVIEKDAYSP 153 Query: 156 ---DNAPLTQIHADWAEAKGFNGAGLKVGVIDTGVDYKHPDIAAAYKG--GYDSFYQDND 210 D+AP I A+ A G+ G G+ V ++DTGVDY HPD+ A+ G+D ++D Sbjct: 154 EMLDSAPF--IGANDAWEAGYTGEGITVAILDTGVDYTHPDLVHAFGDYKGWDFIDNNDD 211 Query: 211 PYEEEPLTPAEDPXXXXXXXXXXXXXXXXTIIGQFAANGNTAQKGVAPGADLYVYKVLGR 270 P E TP DP + G AANG KGVAP A+L Y+VLG Sbjct: 212 PQE----TPPGDPRGIETTHGTH-------VAGTVAANG--LIKGVAPDANLLAYRVLGP 258 Query: 271 NLDKPSTSSGSSAQVIDGIEHAVKDGMDVINLSLGSDSEKDVNSPD---SIAINNAVLSG 327 GS+A VI GIE AV+DG D++NLSLG+ +N PD SIA++ A+ G Sbjct: 259 G------GRGSTAGVIAGIERAVQDGADIMNLSLGN----TLNDPDFATSIALDWAMAEG 308 Query: 328 VTAVIANGNNGEAGYFSLGSPAASQLAISVGSVTSPIDAYSGKFKAEIANSVTSVTYDTY 387 V AV +NGN+G + ++GSP S+ AISVG+ P + K+KA + S Sbjct: 309 VVAVTSNGNSGPNNW-TVGSPGTSRDAISVGATRLPYN----KYKASVFTS--------- 354 Query: 388 FPFHIMAYELEKSDFASADIIG-----------TDPVRVVYANLGADEDYPEGDLSKTIV 436 + D+ SADI+G + +A LG D+ D+ I Sbjct: 355 ----------DGIDYPSADIMGFPSDEELLELDGETYEYAFAGLGKPGDFEGVDVEGKIA 404 Query: 437 LISRGDLTFQDKIANAKAHGVKALVIFNGNARKVDNKSEAILDESYTDRSGFIGINLGNG 496 LI RG++ F +K NAKA G +I+N A G+ Sbjct: 405 LIVRGEIPFVEKAENAKAAGAVGAIIYNNVA----------------------GVQPTVP 442 Query: 497 YQNVPTFDIKGSEGRKLARAIIANGDRPTYFTFGSEYQHEMTTGDTMSTFSSWGPNVDGN 556 +PT + +G K+ R + NG F+ + + G+T++ FSS GP V Sbjct: 443 GLAIPTIMLSNEDGLKM-RNELENGQNTVTFSI----EFDKLVGETVADFSSRGP-VMHT 496 Query: 557 LSIKPDIVAPGDGVLSTYPAYGGDYSKAYQRSSGTSMATPHVAGLSLLIKQAHPEYTPFD 616 IKPD+ APG ++ST P + D Y GTSMA+PHVAG + L+ +AHP + Sbjct: 497 WMIKPDVSAPGVAIVSTIPTHQPDDPYGYGSRQGTSMASPHVAGAAALLLEAHPNWGVDH 556 Query: 617 IRAVLANTADPIL---YNKAPEDLYIQGPGRANVENAILTPALLEAVEPITILDKNYVAQ 673 ++A L NTA+ ++ N+ P + QG G + +AI + L V P Sbjct: 557 VKAALMNTAENLVDENGNRYPHN--TQGAGSIRIVDAIESETL---VTP----------- 600 Query: 674 SVTNYNPSTGFGVLTPGSKSSKTLQ---LKNLSSSDVAYTASVKWNYGDGKVTAALDKTT 730 S FG T Q + NLS+ Y V++ + K Sbjct: 601 ------GSHSFGTFTKERGKQVERQHFTIHNLSNKRKTYQFDVQFAGNPDGIKVKTSK-N 653 Query: 731 LTASAHGTTGFNLNVAVAPDANPEQP--YQGNVILTAEGQPTLHLPFTVHVNDKAEQPDW 788 L T N NV V DA P Y+G +I++ +G T+ +P + V+ +PD+ Sbjct: 654 LRVQPGKTQKINFNVQV--DARKLDPGYYEGTIIVS-DGSQTVEVPTILFVS----EPDY 706 >SPy2010 [O] COG1404 Subtilisin-like serine proteases Length = 1181 Score = 93.6 bits (231), Expect = 2e-18, Method: Compositional matrix adjust. Identities = 143/463 (30%), Positives = 205/463 (44%), Gaps = 102/463 (22%) Query: 292 AVKDGMDVINLSLGSDSEKDVNSPDSI--AINNAVLSGVTAVIANGNNGEAG-------- 341 AV G VIN+S G+ + N PD A + A GV+ V + GN+ G Sbjct: 249 AVNLGAKVINMSFGNAALAYANLPDETKKAFDYAKSKGVSIVTSAGNDSSFGGKTRLPLA 308 Query: 342 ----YFSLGSPAASQLAISVGSVTSPIDAYSGKFKAEIANSVTSVTYDTYFP-FHIMAYE 396 Y +G+PAA+ ++V S SP K E A T+ D P +E Sbjct: 309 DHPDYGVVGTPAAADSTLTVASY-SP-----DKQLTETATVKTADQQDKEMPVLSTNRFE 362 Query: 397 LEKS-DFASADIIGTDPVRVVYANLGADEDYPEGDLSKTIVLISRGDLTFQDKIANAKAH 455 K+ D+A YAN G ED + D+ I LI RGD+ F+DKIANAK Sbjct: 363 PNKAYDYA-------------YANRGMKEDDFK-DVKGKIALIERGDIDFKDKIANAKKA 408 Query: 456 GVKALVIFNGNARKVDNKSEAILDESYTDRSGFIGINLGNGYQNVPTFDIKGSEGRKLAR 515 G ++I+ DN+ + GF I L N Q +P I +G Sbjct: 409 GAVGVLIY-------DNQDK-----------GF-PIELPNVDQ-MPAAFISRKDG----- 443 Query: 516 AIIANGDRPTYFTFGSEYQHEMT-TGDTMSTFSSWGPNVDGNLSIKPDIVAPGDGVLSTY 574 ++ + TF + + T +G +S FSSWG DGN IKPDI APG +LS+ Sbjct: 444 -LLLKENPQKTITFNATPKVLPTASGTKLSRFSSWGLTADGN--IKPDIAAPGQDILSSV 500 Query: 575 PAYGGDYSKAYQRSSGTSMATPHVAG-LSLLIKQ---AHPEYTP---FDI-RAVLANTAD 626 + Y + SGTSM+ P VAG + LL KQ +P+ TP D+ + VL ++A Sbjct: 501 A------NNKYAKLSGTSMSAPLVAGIMGLLQKQYETQYPDMTPSERLDLAKKVLMSSA- 553 Query: 627 PILYNKAPEDLYI----QGPGRANVENAILTPALLEAVEPITILDKNYVAQSVTNYNPST 682 LY++ E Y QG G + + A + + + DK+ + V N S Sbjct: 554 TALYDED-EKAYFSPRQQGAGAVDAKKA--------SAATMYVTDKDNTSSKVHLNNVSD 604 Query: 683 GFGV-LTPGSKSSKTLQLKNLSSSDVAYTASVKWNYGDGKVTA 724 F V +T +KS K +L Y A+V+ + DGK+ A Sbjct: 605 KFEVTVTVHNKSDKPQEL--------YYQATVQTDKVDGKLFA 639 >BS_aprE [O] COG1404 Subtilisin-like serine proteases Length = 381 Score = 86.3 bits (212), Expect = 3e-16, Method: Compositional matrix adjust. Identities = 92/317 (29%), Positives = 143/317 (45%), Gaps = 61/317 (19%) Query: 52 VQLSGQPAAVGKYAA--KQGISALAKTATEAAVKSQQADVLDQAEAKGIDLTVNYKYDTI 109 VQ +G+ + KY KQ +SA++ +++ DV+ + KG + +KY Sbjct: 27 VQAAGKSSTEKKYIVGFKQTMSAMSS--------AKKKDVISE---KGGKVQKQFKY--- 72 Query: 110 LNGFEVTVPADKIPELAKISGVTSIYPNSTWYALPDQTVTETTYRNDNAP--LTQIHADW 167 +N T+ + EL K V Y D E + P ++QI A Sbjct: 73 VNAAAATLDEKAVKELKKDPSVA--------YVEEDHIAHEYA---QSVPYGISQIKAPA 121 Query: 168 AEAKGFNGAGLKVGVIDTGVDYKHPDIAAAYKGGYDSFYQDNDPYEEEPLTPAEDPXXXX 227 ++G+ G+ +KV VID+G+D HPD+ +GG + +PY++ Sbjct: 122 LHSQGYTGSNVKVAVIDSGIDSSHPDLNV--RGGASFVPSETNPYQD------------- 166 Query: 228 XXXXXXXXXXXXTIIGQFAA-NGNTAQKGVAPGADLYVYKVLGRNLDKPSTSSGSSAQVI 286 + G AA N + GV+P A LY KVL ST SG + +I Sbjct: 167 ------GSSHGTHVAGTIAALNNSIGVLGVSPSASLYAVKVL------DSTGSGQYSWII 214 Query: 287 DGIEHAVKDGMDVINLSLGSDSEKDVNSPDSIAINNAVLSGVTAVIANGNNGEAGYFS-L 345 +GIE A+ + MDVIN+SLG + ++ ++ AV SG+ A GN G +G S + Sbjct: 215 NGIEWAISNNMDVINMSLGGPTG---STALKTVVDKAVSSGIVVAAAAGNEGSSGSTSTV 271 Query: 346 GSPAASQLAISVGSVTS 362 G PA I+VG+V S Sbjct: 272 GYPAKYPSTIAVGAVNS 288 Score = 52.4 bits (124), Expect = 5e-06, Method: Compositional matrix adjust. Identities = 32/64 (50%), Positives = 40/64 (62%), Gaps = 6/64 (9%) Query: 562 DIVAPGDGVLSTYPAYGGDYSKAYQRSSGTSMATPHVAGLSLLIKQAHPEYTPFDIRAVL 621 D++APG + ST P GG Y AY +GTSMATPHVAG + LI HP +T +R L Sbjct: 303 DVMAPGVSIQSTLP--GGTYG-AY---NGTSMATPHVAGAAALILSKHPTWTNAQVRDRL 356 Query: 622 ANTA 625 +TA Sbjct: 357 ESTA 360 >BS_ispA [O] COG1404 Subtilisin-like serine proteases Length = 319 Score = 79.3 bits (194), Expect = 4e-14, Method: Compositional matrix adjust. Identities = 70/198 (35%), Positives = 96/198 (48%), Gaps = 29/198 (14%) Query: 167 WAEAKGFNGAGLKVGVIDTGVDYKHPDIAAAYKGGYDSFYQDNDPYEEEPLTPAEDPXXX 226 WA KG G +KV V+DTG D HPD+ GG + + D+D +E+ ++ Sbjct: 35 WA--KGVKGKNIKVAVLDTGCDTSHPDLKNQIIGGKN--FTDDDGGKEDAIS-------- 82 Query: 227 XXXXXXXXXXXXXTIIGQFAAN-GNTAQKGVAPGADLYVYKVLGRNLDKPSTSSGSSAQV 285 + G AAN N GVAP A L + KVLG SG + Sbjct: 83 ------DYNGHGTHVAGTIAANDSNGGIAGVAPEASLLIVKVLGG-----ENGSGQYEWI 131 Query: 286 IDGIEHAVKDGMDVINLSLGSDSEKDVNSPDSIAINNAVLSGVTAVIANGNNGEAGYFS- 344 I+GI +AV+ +D+I++SLG S DV A+ NAV +GV V A GN G+ + Sbjct: 132 INGINYAVEQKVDIISMSLGGPS--DVPELKE-AVKNAVKNGVLVVCAAGNEGDGDERTE 188 Query: 345 -LGSPAASQLAISVGSVT 361 L PAA I+VGSV+ Sbjct: 189 ELSYPAAYNEVIAVGSVS 206 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.311 0.129 0.360 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 92,610,062 Number of extensions: 3965724 Number of successful extensions: 5971 Number of sequences better than 1.0e-05: 20 Number of HSP's gapped: 5949 Number of HSP's successfully gapped: 27 Length of query: 1353 Length of database: 38,378,431 Length adjustment: 119 Effective length of query: 1234 Effective length of database: 24,653,685 Effective search space: 30422647290 Effective search space used: 30422647290 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (21.8 bits) S2: 122 (51.6 bits)