BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0290.0036.p (585 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value CAC2755 [V] COG1132 ABC-type multidrug transport system, ATPase ... 633 0.0 BS_yheI [V] COG1132 ABC-type multidrug transport system, ATPase ... 416 e-116 STM1256 [EP] COG0601 ABC-type dipeptide/oligopeptide/nickel tran... 377 e-104 TM1256 [V] COG1132 ABC-type multidrug transport system, ATPase a... 377 e-104 ZmdlA [V] COG1132 ABC-type multidrug transport system, ATPase an... 354 2e-97 >CAC2755 [V] COG1132 ABC-type multidrug transport system, ATPase and permease components Length = 576 Score = 633 bits (1633), Expect = 0.0, Method: Compositional matrix adjust. Identities = 303/578 (52%), Positives = 424/578 (73%), Gaps = 2/578 (0%) Query: 1 MDFSVLLRFFKAHKFSYLTGFAFMFAASFIQTLFPRVLGSAVDLMKESGFAVRQVMFQVL 60 M+ ++LL FF +KF Y+ G F+F +S+IQTLFP+VLG+ +D++K++ F++ V +L Sbjct: 1 MNKNILLNFFYRNKFKYIIGLIFVFLSSYIQTLFPKVLGNTIDILKKNNFSMISVKKNIL 60 Query: 61 WMTLIAAAVFACTFLWRNIIIANARNLECYFREELVRHFLKLSPAFYNTRKTGDLIAYAI 120 ++ LIA F T++WRN+II R LECY RE+L F +LSP FYN K G+LIAYAI Sbjct: 61 YIVLIAFFTFLLTYIWRNLIIGTGRTLECYLREKLFNKFQELSPEFYNNEKVGNLIAYAI 120 Query: 121 NDISAVRMTFGPATAMSFNGIVICFSSVYFMFATVDARLTLITLAPLPFIILLMLFTGRK 180 NDISAVR+TFGP+ AM+ NG+ +C SS+Y M ++ +LTL+ LAP+PFI+++M+ R Sbjct: 121 NDISAVRITFGPSIAMTVNGLTVCLSSIYSMIFSISLKLTLLCLAPIPFIVIIMIKISRL 180 Query: 181 VQTRFRIVQNQFGAVSDRVQENISGIRVIKAYVQERSEMERFSALSSRMKQANLDLIRVS 240 ++ RF VQ FG +SDRV ENI+GIRVIK+YV+E E+E F L+ M ANL ++RVS Sbjct: 181 IRARFTTVQESFGNISDRVNENINGIRVIKSYVEEEKEVENFEKLNDAMAIANLRMVRVS 240 Query: 241 AALPAMIEFGFAICFVLNLVFGSRMVLRGEISIGMFVAFNGYLSLIVNPIVSIGRIVTIF 300 + L +IE F++ F NL+ G MVL+G +S+G FVAFN YL++I+ P++SIGRI+ IF Sbjct: 241 STLSPIIELCFSLSFCANLIIGGNMVLKGTVSLGDFVAFNTYLAMIMAPVMSIGRILVIF 300 Query: 301 QRGMASLGRLHDILRIQPDIQDLPQAADIQPEGPVELRDLTFHYDGTGTPALKDISLVLP 360 QRGMASL RL+ I + DI D ++ +I +G +E+R+L+F Y T LK+I+L + Sbjct: 301 QRGMASLKRLNKIFSTKKDILDGEKSINIPLKGNIEIRNLSFKYKDTDIKTLKNINLSIK 360 Query: 361 KGHTLGIIGHTGSGKSTLASLLFRFFNVEPGRILLNGRXXXXNGYTLEALRGGLGYVPQD 420 KG +GIIG TGSGKSTL +LL + +NVE G I +GR N YTL +R +GYVPQD Sbjct: 361 KGELIGIIGKTGSGKSTLVNLLLKLYNVENGSIAFDGR--DINDYTLACIRNNIGYVPQD 418 Query: 421 TFIFAASVRENIVFFKDGYSEEEVREAARLSMIADSIGGLPEGYDTILGERGVNLSGGQK 480 F+F++++++NI FFKD Y + V +AA++S I D+I +G++T+LGERG+NLSGGQK Sbjct: 419 NFLFSSTIKDNIKFFKDVYDDAAVEKAAKISCIYDTILKFKDGFNTVLGERGINLSGGQK 478 Query: 481 QRLAIARALIRDPAILILDDALSAVDAVTEGLILERLRQTRQGKTNILISHRVSAVMEAD 540 QR++IARA+I+DP ILILDDALSAVD +TE I+E L+ R KT+I+I+HR+SAV AD Sbjct: 479 QRVSIARAVIKDPPILILDDALSAVDTITETNIIENLKLFRNEKTSIIIAHRLSAVKNAD 538 Query: 541 EIIVLDKGMIVERGTHAQLLKEGGLYYDIYTEQHEDGQ 578 +IIVL+KG I E G+H +LL +GG+YYDIY EQ+ED + Sbjct: 539 KIIVLNKGEIAEIGSHKELLAKGGIYYDIYKEQYEDKK 576 >BS_yheI [V] COG1132 ABC-type multidrug transport system, ATPase and permease components Length = 585 Score = 416 bits (1070), Expect = e-116, Method: Compositional matrix adjust. Identities = 217/582 (37%), Positives = 347/582 (59%), Gaps = 19/582 (3%) Query: 3 FSVLLR---FFKAHKFSYLTGFAFMFAASFIQTLFPRVLGSAVDLMKESGFAVRQVMFQV 59 FSVL + FFKA+ Y + A + I+ P++LG+A+D MK F ++F + Sbjct: 2 FSVLKKLGWFFKAYWLRYTIAIVLLLAVNVIEMFPPKLLGNAIDDMKAGAFTAEGLLFYI 61 Query: 60 LWMTLIAAAVFACTFLWRNIIIANARNLECYFREELVRHFLKLSPAFYNTRKTGDLIAYA 119 ++ AAV+ ++ W + + A +E R +L+ H L +SP FY +TGDL+A Sbjct: 62 GIFFVLTAAVYIMSYFWMHQLFGGANLMEKILRTKLMGHLLTMSPPFYEKNRTGDLMARG 121 Query: 120 INDISAVRMTFGPATAMSFNGIVICFSSVYFMFAT-------VDARLTLITLAPLPFIIL 172 ND+ AV +T G GI+ S FM + +LT + PLP + + Sbjct: 122 TNDLQAVSLTTG-------FGILTLVDSTMFMMTIFLTMGFLISWKLTFAAIIPLPVMAI 174 Query: 173 LMLFTGRKVQTRFRIVQNQFGAVSDRVQENISGIRVIKAYVQERSEMERFSALSSRMKQA 232 + G K+ RF QN FGA++DRV E++SG+RVI+AYVQE +++ RF+ +++ + Q Sbjct: 175 AISLYGSKIHERFTEAQNAFGALNDRVLESVSGVRVIRAYVQETNDVRRFNEMTADVYQK 234 Query: 233 NLDLIRVSAALPAMIEFGFAICFVLNLVFGSRMVLRGEISIGMFVAFNGYLSLIVNPIVS 292 N+ + + + ++ +++ L +G+ +V R E+++G V+FN YL +++ P+ + Sbjct: 235 NMKVAFIDSLFEPTVKLLVGASYLIGLGYGAFLVFRNELTLGELVSFNVYLGMMIWPMFA 294 Query: 293 IGRIVTIFQRGMASLGRLHDILRIQPDIQDLPQAADIQPEGPVELRDLTFHYDGTGTPAL 352 IG ++ + QRG ASL R+++ L + D+ D Q AD++ G + ++F Y + + L Sbjct: 295 IGELINVMQRGNASLDRVNETLSYETDVTDPKQPADLKEPGDIVFSHVSFTYPSSTSDNL 354 Query: 353 KDISLVLPKGHTLGIIGHTGSGKSTLASLLFRFFNVEPGRILLNGRXXXXNGYTLEALRG 412 +DIS + KG T+GI G TGSGK+T+ L R + G I +G L+ LRG Sbjct: 355 QDISFTVRKGQTVGIAGKTGSGKTTIIKQLLRQYPPGEGSITFSG--VPIQQIPLDRLRG 412 Query: 413 GLGYVPQDTFIFAASVRENIVFFKDGYSEEEVREAARLSMIADSIGGLPEGYDTILGERG 472 +GYVPQD +F+ +V+ENI++ K +++EV++A + + LP G +T++GE+G Sbjct: 413 WIGYVPQDHLLFSRTVKENILYGKQDATDKEVQQAIAEAHFEKDLHMLPSGLETMVGEKG 472 Query: 473 VNLSGGQKQRLAIARALIRDPAILILDDALSAVDAVTEGLILERLRQTRQGKTNILISHR 532 V LSGGQKQR++IARAL+ +P ILILDD+LSAVDA TE I++ +R+ R+GKT +++HR Sbjct: 473 VALSGGQKQRISIARALMANPEILILDDSLSAVDAKTEAAIIKNIRENRKGKTTFILTHR 532 Query: 533 VSAVMEADEIIVLDKGMIVERGTHAQLLKEGGLYYDIYTEQH 574 +SAV AD I+V+D G+I ERGTH +LL G Y + Y Q Sbjct: 533 LSAVEHADLILVMDGGVIAERGTHQELLANNGWYREQYERQQ 574 >STM1256 [EP] COG0601 ABC-type dipeptide/oligopeptide/nickel transport systems, permease components Length = 324 Score = 377 bits (968), Expect = e-104, Method: Compositional matrix adjust. Identities = 0/570 (0%), Positives = 0/570 (0%), Gaps = 2/570 (0%) Query: 4 SVLLRFFKAHKFSYLTGFAFMFAASFIQTLFPRVLGSAVDLMKESGFAVRQVMFQVLWMT 63 G + + S + + + + + E + + Sbjct: 5 FSHFLRLIILLVLVAAGTFILLSFSPVDPIRAYIGNDLLHVPPEQYARIAARWGLDQPLW 64 Query: 64 LIAAAVFACTFLWRNIIIANARNLECYFREELVRHFLKLSPAFYNTRKTGDLIAYAINDI 123 F E L + + + Sbjct: 65 ERFGHWFWRLLQGDMGYSMLFNMPVASVIRERFATSFALLAGAWLLSGVLGVTLGFLAGR 124 Query: 124 SAVRMTFGPATAMSFNGIVICFSSVYFMFATVDARLTLITLAPLPFIILLMLFTGRKVQT 183 R +S+ + + + + A + + T + Sbjct: 125 FLHRWPDKMICRISYLLSSLPTFWIAMLLLALFAVRWPVLPVCCAWDPGNNAGTALLSER 184 Query: 184 RFRIVQNQFGAVSDRVQENISGIRVIKAYVQERSEMERFSALSSRMKQANLDLIRVSAAL 243 +V + + R A V + + A + + A Sbjct: 185 LRHLVLPVCALSLLGMGQIALHTREKIASVMKSEFIRFARAQGDKGWSLLRHQVLRHAIT 244 Query: 244 PAMIEFGFAICFVLNLVFGSRMVLRGEISIGMFVAFNGYLSLIVNPIVSIGRIVTIFQRG 303 PA+ ++ ++ + V + + + + + + +F Sbjct: 245 PALCLQFASLGELMGGALLAEKVFAYPGLGQATIDAGLRGDVPLLMGIVLFCTLLVFAGN 304 Query: 304 MASLGRLHDILRIQPDIQDLPQAADIQPEGPVELRDLTFHYDGTGTPALKDISLVLPKGH 363 Sbjct: 305 364 Query: 364 TLGIIGHTGSGKSTLASLLFRFFNVEPGRILLNGRXXXXNGYTLEALRGGLGYVPQDTFI 423 Sbjct: 365 -- 422 Query: 424 FAASVRENIVFFKDGYSEEEVREAARLSMIADSIGGLPEGYDTILGERGVNLSGGQKQRL 483 Sbjct: 423 482 Query: 484 AIARALIRDPAILILDDALSAVDAVTEGLILERLRQTRQGKTNILISHRVSAVMEADEII 543 Sbjct: 483 542 Query: 544 VLDKGMIVERGTHAQLLKEGGLYYDIYTEQ 573 Sbjct: 543 572 >TM1256 [V] COG1132 ABC-type multidrug transport system, ATPase and permease components Length = 577 Score = 377 bits (968), Expect = e-104, Method: Compositional matrix adjust. Identities = 199/570 (34%), Positives = 322/570 (56%), Gaps = 2/570 (0%) Query: 4 SVLLRFFKAHKFSYLTGFAFMFAASFIQTLFPRVLGSAVDLMKESGFAVRQVMFQVLWMT 63 S+L+ F K + YL G + A +Q PRV+G VD++ + + W+ Sbjct: 5 SILVDFLKRNWHKYLLGVLSLIAVDLLQVYIPRVIGKIVDMLNTETMNFDTLKIMLFWVM 64 Query: 64 LIAAAVFACTFLWRNIIIANARNLECYFREELVRHFLKLSPAFYNTRKTGDLIAYAINDI 123 A+ +F F WR + AR ++ L L+P F++ K+G+L+A ND+ Sbjct: 65 AAASGMFIGRFFWRFFLGGTARKFFLETSNKMFSKLLSLTPGFFDRMKSGELMARFTNDL 124 Query: 124 SAVRMTFGPATAMSFNGIVICFSSVYFMFATVDARLTLITLAPLPFIILLMLFTGRKVQT 183 S +R G + F+ ++ +FM V +L+ I+ APL + + + GR + Sbjct: 125 SLLRRVLGQGAILFFDSFIMIVMVFFFMIGNVGWKLSWISFAPLLLLAPVSMSFGRLIHR 184 Query: 184 RFRIVQNQFGAVSDRVQENISGIRVIKAYVQERSEMERFSALSSRMKQANLDLIRVSAAL 243 + VQ F +S +E IS +R++K++ E + F + + + L I+VS+ Sbjct: 185 KVSEVQRSFSELSGFTEETISSVRIVKSFSVEDVSFKIFENRALKNYEDTLSAIKVSSVF 244 Query: 244 PAMIEFGFAICFVLNLVFGSRMVLRGEISIGMFVAFNGYLSLIVNPIVSIGRIVTIFQRG 303 +I + F L L++G R V+ G+IS+G F+AFN YL ++V P+++ G +V +FQRG Sbjct: 245 RPLINLIASTAFFLALLYGGRAVINGKISLGDFIAFNSYLGMLVWPMMAYGFMVDLFQRG 304 Query: 304 MASLGRLHDILRIQPDIQDLPQAADIQPEGPVELRDLTFHYDGTGTPALKDISLVLPKGH 363 A++ RL I QP++++ + I E+R+LT+ Y GT LK++++ + +G Sbjct: 305 RAAMRRLDTIFTAQPEVKEPEKPIRIDHFESFEVRNLTYRYPGTEREVLKNVTMKINRGE 364 Query: 364 TLGIIGHTGSGKSTLASLLFRFFNVEPGRILLNGRXXXXNGYTLEALRGGLGYVPQDTFI 423 +GI G GSGKST+A LL + + VE G+I +NG N + E +R + VPQ+TF+ Sbjct: 365 MIGIAGSVGSGKSTIAKLLVKLYPVERGKIFING--VDINDVSSENIRSIVTLVPQETFL 422 Query: 424 FAASVRENIVFFKDGYSEEEVREAARLSMIADSIGGLPEGYDTILGERGVNLSGGQKQRL 483 F+ +VR NI + E+++ E +L+ + D I PE +DTI+GERGV LSGGQKQR+ Sbjct: 423 FSDTVRNNITVGLENVDEKKIEEVTKLAAVYDDIMSFPEKFDTIVGERGVTLSGGQKQRI 482 Query: 484 AIARALIRDPAILILDDALSAVDAVTEGLILERLRQTRQGKTNILISHRVSAVMEADEII 543 IARALIRD + I DD LSAVD TE I++ +R+ +GKT ++I+HR+ + AD I Sbjct: 483 TIARALIRDFEVYIFDDCLSAVDPETEERIIDSIRKGMKGKTIVVITHRLKVLKNADRIY 542 Query: 544 VLDKGMIVERGTHAQLLKEGGLYYDIYTEQ 573 VL +G ++E GTH +L+ G+Y +Y +Q Sbjct: 543 VLHEGRVIEEGTHEELMIRDGMYSRMYRKQ 572 >ZmdlA [V] COG1132 ABC-type multidrug transport system, ATPase and permease components Length = 590 Score = 354 bits (909), Expect = 2e-97, Method: Compositional matrix adjust. Identities = 205/575 (35%), Positives = 317/575 (55%), Gaps = 9/575 (1%) Query: 3 FSVLLRFFKAHKFSYLTGFAFMFAASFIQTLFPRVLGSAVDLMKESGFAVRQVMFQVLWM 62 F+ L +F+ YL A + + +Q + P+V+G VD + E F Q++ + M Sbjct: 4 FAQLSWYFRREWRRYLGAVALLVIIAMLQLVPPKVVGIVVDGVTEQHFTTGQILMWIATM 63 Query: 63 TLIAAAVFACTFLWRNIIIANARNLECYFREELVRHFLKLSPAFYNTRKTGDLIAYAIND 122 LIA V+ ++WR ++ + L RE+ R + P FY +TGDL+A A ND Sbjct: 64 VLIAVVVYLLRYVWRVLLFGASYQLAVELREDYYRQLSRQHPEFYLRHRTGDLMARATND 123 Query: 123 ISAVRMTFGPATAMSFNGIVICFSSVYFMFATVDARLTLITLAPLPFIILLMLFTGRKVQ 182 + V G + +V+ + + M + +LTL L P+P + +++ G + Sbjct: 124 VDRVVFAAGEGVLTLVDSLVMGCAVLIMMSTQISWQLTLFALLPMPVMAIMIKRNGDALH 183 Query: 183 TRFRIVQNQFGAVSDRVQENISGIRVIKAYVQERSEMERFSALSSRMKQANLDLIRVSAA 242 RF++ Q F +++DR QE+++ IR+IKA+ E + F+A + + N+ + R+ A Sbjct: 184 ERFKLAQAAFSSLNDRTQESLTSIRMIKAFGLEDRQSALFAADAEDTGKKNMRVARIDAR 243 Query: 243 LPAMIEFGFAICFVLNLVFGSRMVLRGEISIGMFVAFNGYLSLIVNPIVSIGRIVTIFQR 302 I + +L + GS MV++G +++G +F YL L++ P++++ + I +R Sbjct: 244 FDPTIYIAIGMANLLAIGGGSWMVVQGSLTLGQLTSFMMYLGLMIWPMLALAWMFNIVER 303 Query: 303 GMASLGRLHDILRIQPDIQDLPQAADIQPEGPVELRDLTFH---YDGTGTPALKDISLVL 359 G A+ R+ +L P + D ++ PEG EL D+ H Y T PAL++++ L Sbjct: 304 GSAAYSRIRAMLAEAPVVND---GSEPVPEGRGEL-DVNIHQFTYPQTDHPALENVNFAL 359 Query: 360 PKGHTLGIIGHTGSGKSTLASLLFRFFNVEPGRILLNGRXXXXNGYTLEALRGGLGYVPQ 419 G LGI G TGSGKSTL SL+ R F+V G I + L++ R L V Q Sbjct: 360 KPGQMLGICGPTGSGKSTLLSLIQRHFDVSEGDIRFHD--IPLTKLQLDSWRSRLAVVSQ 417 Query: 420 DTFIFAASVRENIVFFKDGYSEEEVREAARLSMIADSIGGLPEGYDTILGERGVNLSGGQ 479 F+F+ +V NI +++E+ ARL+ + D I LP+GYDT +GERGV LSGGQ Sbjct: 418 TPFLFSDTVANNIALGCPNATQQEIEHVARLASVHDDILRLPQGYDTEVGERGVMLSGGQ 477 Query: 480 KQRLAIARALIRDPAILILDDALSAVDAVTEGLILERLRQTRQGKTNILISHRVSAVMEA 539 KQR++IARAL+ + ILILDDALSAVD TE IL LRQ QG+T I+ +HR+SA+ EA Sbjct: 478 KQRISIARALLVNAEILILDDALSAVDGRTEHQILHNLRQWGQGRTVIISAHRLSALTEA 537 Query: 540 DEIIVLDKGMIVERGTHAQLLKEGGLYYDIYTEQH 574 EIIV+ G I +RG H L ++ G Y D+Y Q Sbjct: 538 SEIIVMQHGHIAQRGNHDVLAQQSGWYRDMYRYQQ 572 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.327 0.142 0.407 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 40,534,191 Number of extensions: 1703041 Number of successful extensions: 11227 Number of sequences better than 1.0e-05: 1902 Number of HSP's gapped: 8847 Number of HSP's successfully gapped: 2066 Length of query: 585 Length of database: 38,378,431 Length adjustment: 112 Effective length of query: 473 Effective length of database: 25,461,023 Effective search space: 12043063879 Effective search space used: 12043063879 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.7 bits) S2: 119 (50.4 bits)