BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0290.0035.p (543 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value CAC2756 [V] COG1132 ABC-type multidrug transport system, ATPase ... 570 e-162 BS_yheH [V] COG1132 ABC-type multidrug transport system, ATPase ... 334 2e-91 SP1839 [V] COG1132 ABC-type multidrug transport system, ATPase a... 322 1e-87 TM0043 [V] COG1132 ABC-type multidrug transport system, ATPase a... 318 1e-86 BS_yknV [V] COG1132 ABC-type multidrug transport system, ATPase ... 304 2e-82 >CAC2756 [V] COG1132 ABC-type multidrug transport system, ATPase and permease components Length = 592 Score = 570 bits (1469), Expect = e-162, Method: Compositional matrix adjust. Identities = 285/535 (53%), Positives = 367/535 (68%) Query: 1 LLQRVIDHFLIGGAVERGLDSIGGXXXXXXXXXXXXXXXXXXQALIVGKAGQSLIHELRV 60 +L+ ID FL+G V+ G SI +A ++ A QS++ LR Sbjct: 53 ILKVSIDDFLVGKRVQSGFYSISSMGFLYFGVVALGGFFSISEANLINSAAQSIMKGLRS 112 Query: 61 RVFSIIERLPLPYLDRTSSGRLITRATNDTSEISDLYTDVIISLVKDVLLLAGIVYAMLV 120 VF I+ LPL YLD+TSSG LITRATND +S++YTDVIISL +D+ LL GI+YAML Sbjct: 113 SVFKTIQLLPLSYLDKTSSGSLITRATNDVEALSEMYTDVIISLFQDIFLLLGIIYAMLS 172 Query: 121 LSPKLTLVSXXXXXXXXXXXXXXXXXXXXXXXHMKHYIGQINGFIAESISGMRVIQIFRA 180 L+ +L+L+S MK IG+INGF+AE++SGM+++QIF A Sbjct: 173 LNIELSLISFSVIPIMFFLVFSLKTRIKRNFAKMKSLIGKINGFMAENLSGMKLVQIFTA 232 Query: 181 EKEKEQQFLKLNGEYFNTTLIQVRLNSILKPASDMFQSLAIAILVWYSVGRIAGGTLQIG 240 EKEK ++F LN EYFN TL QVRLNSIL+PAS++FQ+L+IA+L+WY + +IA T+QIG Sbjct: 233 EKEKRREFKALNNEYFNATLFQVRLNSILRPASNVFQNLSIALLLWYGMAKIANHTIQIG 292 Query: 241 VLYAFTTYIKQFFAPVSDLADKYTSIQSAFVSTXXXXXXXXXXXXXXXPDAGLPMQRLNG 300 VLYAFT+YI+QFF P+SDLAD YT+IQSA VS G+PM+++ G Sbjct: 293 VLYAFTSYIRQFFDPISDLADSYTTIQSALVSADRIFELLDKKIILEDLHKGIPMKKIEG 352 Query: 301 RIEFRRVWFSYNGADWVLKDVSFVIEKGQTAAFIGETGAGKTTIISLINGFYKVQKGEIL 360 +EF+ VWFSY+ +++LK ++F I KG+TAAF+GETGAGKTTIISLI+GFYKVQKGEIL Sbjct: 353 DVEFKDVWFSYDNKNFILKGLNFKINKGETAAFVGETGAGKTTIISLISGFYKVQKGEIL 412 Query: 361 IDGVNVNDIRLDDLRRNISVVLQDVFLFSGTIRXXXXXXXXXXXXXXXXALRASCAEAFV 420 IDGVN+N IR DLR+N++VVLQDVFLFSG I+ A+ AS A+ F+ Sbjct: 413 IDGVNINSIRPSDLRKNVAVVLQDVFLFSGNIKSNITLNDFVPEDVINSAVEASFAKDFI 472 Query: 421 HSAPEGIDEPVTERGSTLSAGQRQLISFARAIAHDPAIFVLDEATANIDTRTEKLIQQAI 480 P ++EPV ERG T SAGQ+QL+SFARAIAH P+IFVLDEATANIDT TEKLIQ+AI Sbjct: 473 EKLPNKLNEPVMERGKTFSAGQKQLLSFARAIAHTPSIFVLDEATANIDTYTEKLIQKAI 532 Query: 481 DNAASGRTTLIIAHRLSTITGADLIIAMKAGRIAESGHPRELLKRGGYYAKLLQE 535 +N +TT IIAHRLSTI AD I+ + G+I E G+ EL+K+ GYY L+ E Sbjct: 533 ENITRDKTTFIIAHRLSTIRNADKILVIDNGKIVEMGNHNELMKKDGYYRNLVYE 587 >BS_yheH [V] COG1132 ABC-type multidrug transport system, ATPase and permease components Length = 673 Score = 334 bits (857), Expect = 2e-91, Method: Compositional matrix adjust. Identities = 191/504 (37%), Positives = 288/504 (57%), Gaps = 10/504 (1%) Query: 43 QALIVGKAGQSLIHELRVRVFSIIERLPLPYLDRTSSGRLITRATNDTSEISDLYTDVII 102 Q ++ + +I ++R VFS I+++P+ Y D +G+++ R TNDT I DLY V+ Sbjct: 170 QHYLLQMSANRIIQKMRQDVFSHIQKMPIRYFDNLPAGKVVARITNDTEAIRDLYVTVLS 229 Query: 103 SLVKDVLLLAGIVYAMLVLSPKLTLVSXXXXXXXXXXXXXXXXXXXXXXXHMKHYIGQIN 162 + V + + GI A+ +L KL V ++ IN Sbjct: 230 TFVTSGIYMFGIFTALFLLDVKLAFVCLAIVPIIWLWSVIYRRYASYYNQKIRSINSDIN 289 Query: 163 GFIAESISGMRVIQIFRAEKEKEQQFLKLNGEYFNTTLIQVRLNSILKPA-SDMFQSLAI 221 + ESI GM +IQ FR +KE ++F +LN +F + LNS++ ++ ++LA Sbjct: 290 AKMNESIQGMTIIQAFRHQKETMREFEELNESHFYFQNRMLNLNSLMSHNLVNVIRNLAF 349 Query: 222 AILVWYSVGRI--AGGTLQIGVLYAFTTYIKQFFAPVSDLADKYTSIQSAFVSTXXXXXX 279 L+W+ G A G + IGVLYAF Y+ + F P++ + ++++ ++ A VS Sbjct: 350 VCLIWHFGGASLNAAGIVSIGVLYAFVDYLNRLFQPITGIVNQFSKLELARVSAGRVFEL 409 Query: 280 XXXXXXXXXPDAGLPM-QRLNGRIEFRRVWFSYNGADWVLKDVSFVIEKGQTAAFIGETG 338 +AG P +R GR+EFR V F+Y + VLK +SF +KG+T A +G TG Sbjct: 410 LEEKNT---EEAGEPAKERALGRVEFRDVSFAYQEGEEVLKHISFTAQKGETVALVGHTG 466 Query: 339 AGKTTIISLINGFYKVQKGEILIDGVNVNDIRLDDLRRNISVVLQDVFLFSGTI-RXXXX 397 +GK++I++L+ FY QKG++LIDG ++ ++ +LR ++ +VLQD +LFSGTI Sbjct: 467 SGKSSILNLLFRFYDAQKGDVLIDGKSIYNMSRQELRSHMGIVLQDPYLFSGTIGSNVSL 526 Query: 398 XXXXXXXXXXXXALRASCAEAFVHSAPEGIDEPVTERGSTLSAGQRQLISFARAIAHDPA 457 ALR AE + P+GI+EPV E+GSTLS+G+RQLISFARA+A DPA Sbjct: 527 DDERMTEEEIKNALRQVGAEPLLKKLPKGINEPVIEKGSTLSSGERQLISFARALAFDPA 586 Query: 458 IFVLDEATANIDTRTEKLIQQAIDNAASGRTTLIIAHRLSTITGADLIIAMKAGRIAESG 517 I +LDEATA+IDT TE +IQ+A+D GRTT +IAHRLSTI AD I+ + G I E G Sbjct: 587 ILILDEATAHIDTETEAVIQKALDVVKQGRTTFVIAHRLSTIRNADQILVLDKGEIVERG 646 Query: 518 HPRELLKRGGYYAKL--LQESRDH 539 + EL+ G Y ++ LQ+ + H Sbjct: 647 NHEELMALEGQYYQMYELQKGQKH 670 >SP1839 [V] COG1132 ABC-type multidrug transport system, ATPase and permease components Length = 583 Score = 322 bits (825), Expect = 1e-87, Method: Compositional matrix adjust. Identities = 173/489 (35%), Positives = 273/489 (55%), Gaps = 2/489 (0%) Query: 45 LIVGKAGQSLIHELRVRVFSIIERLPLPYLDRTSSGRLITRATNDTSEISDLYTDVIISL 104 L+ + S++ ++R F+ +E+L + Y D+T +G +++R TNDT ISD+++ ++ S Sbjct: 84 LLFARVSYSIVRDIRRDAFANMEKLGMSYFDKTPAGSIVSRLTNDTETISDMFSGILSSF 143 Query: 105 VKDVLLLAGIVYAMLVLSPKLTLVSXXXXXXXXXXXXXXXXXXXXXXXHMKHYIGQINGF 164 + V + +Y MLVL +LT + + + IN Sbjct: 144 ISAVFIFLTTLYTMLVLDFRLTALVLLFLPLIFLLVNLYRKKSVKIIEKTRSLLSDINSK 203 Query: 165 IAESISGMRVIQIFRAEKEKEQQFLKLNGEYFNTTLIQVRLNSI-LKPASDMFQSLAIAI 223 +AE+I G+R+IQ F EK + +F ++N E+ V L+++ L+PA + + L A+ Sbjct: 204 LAENIEGIRIIQAFNQEKRLQAEFDEINQEHLVYANRSVALDALFLRPAMSLLKLLGYAV 263 Query: 224 LVWYSVGRIAGGTLQIGVLYAFTTYIKQFFAPVSDLADKYTSIQSAFVSTXXXXXXXXXX 283 L+ Y R + +G +YAF YI + F P+ ++ ++++Q+A VS Sbjct: 264 LMAYFGYRGFSIGITVGTMYAFIQYINRLFDPLIEVTQNFSTLQTAMVSAGRVFALIDER 323 Query: 284 XXXXXPDAGLPMQRLNGRIEFRRVWFSYNGADWVLKDVSFVIEKGQTAAFIGETGAGKTT 343 + G + G I F V FSY+G +L D+SF + KG+T AF+G TG+GK++ Sbjct: 324 TYEPLQENGQAKVQ-EGNIRFEHVCFSYDGKHPILDDISFSVNKGETIAFVGHTGSGKSS 382 Query: 344 IISLINGFYKVQKGEILIDGVNVNDIRLDDLRRNISVVLQDVFLFSGTIRXXXXXXXXXX 403 II+++ FY+ Q G +L+D V++ D ++LR+NI +VLQ+ FL+ GTI+ Sbjct: 383 IINVLMRFYEFQSGRVLLDDVDIRDFSQEELRKNIGLVLQEPFLYHGTIKSNIAMYQETS 442 Query: 404 XXXXXXALRASCAEAFVHSAPEGIDEPVTERGSTLSAGQRQLISFARAIAHDPAIFVLDE 463 A A++F+ P+G D PV+ERGS+ S GQRQL++FAR +A P I +LDE Sbjct: 443 DEQVQAAAAFVDADSFIQELPQGYDSPVSERGSSFSTGQRQLLAFARTVASQPKILILDE 502 Query: 464 ATANIDTRTEKLIQQAIDNAASGRTTLIIAHRLSTITGADLIIAMKAGRIAESGHPRELL 523 ATANID+ TE L+Q ++ GRTT+ IAHRLSTI A+ I + GRI ESG ELL Sbjct: 503 ATANIDSETESLVQASLAKMRQGRTTIAIAHRLSTIQDANCIYVLDKGRIIESGTHEELL 562 Query: 524 KRGGYYAKL 532 GG Y K+ Sbjct: 563 ALGGTYHKM 571 >TM0043 [V] COG1132 ABC-type multidrug transport system, ATPase and permease components Length = 581 Score = 318 bits (815), Expect = 1e-86, Method: Compositional matrix adjust. Identities = 176/490 (35%), Positives = 269/490 (54%), Gaps = 2/490 (0%) Query: 46 IVGKAGQSLIHELRVRVFSIIERLPLPYLDRTSSGRLITRATNDTSEISDLYTDVIISLV 105 + G AG ++ +R VFS + LP + SG + TR NDT I + ++ V+ S++ Sbjct: 87 VTGYAGSRIVLLMRKDVFSHVISLPYSFFTGIPSGVVTTRIVNDTQNIQEFFSSVVTSVI 146 Query: 106 KDVLLLAGIVYAMLVLSPKLTLVSXXXXXXXXXXXXXXXXXXXXXXXHMKHYIGQINGFI 165 DVLLL +V +++ LS +L ++ + ++N F+ Sbjct: 147 MDVLLLGAVVVSLVQLSRELFGHVYYLLPLIVISILLFRYFDRRAYRKVRTNLARLNAFL 206 Query: 166 AESISGMRVIQIFRAEKEKEQQFLKLNGEYFNTTLIQVRLNSILKPASDMFQSLAIAILV 225 AE I+G+ V +I EK KE++F + EY+ + + Q+ + + +P D L +++++ Sbjct: 207 AEHIAGINVTKILNLEKHKEREFSTVATEYYRSLMEQLYVFGVFRPLMDFLYFLGVSLVL 266 Query: 226 WYSVGRIAGGTLQIGVLYAFTTYIKQFFAPVSDLADKYTSIQSAFVSTXXXXXXXXXXXX 285 WY IA TL G LYAF +Y+ FF P+ DL++KY IQ++ S+ Sbjct: 267 WYGARYIANKTLGFGSLYAFVSYLDMFFRPLRDLSEKYDIIQNSMASSEKILNLLKEEPE 326 Query: 286 XXXPDAGLPMQRLNGRIEFRRVWFSYNGADWVLKDVSFVIEKGQTAAFIGETGAGKTTII 345 G P + GR+ F VWFSY+G +WVLKD++ + G+ A +GETG GK+T++ Sbjct: 327 VIGNPNG-PNKISKGRVRFENVWFSYDGKNWVLKDINLDFQPGKLYAIVGETGGGKSTLM 385 Query: 346 SLINGFYKVQKGEILIDGVNVNDIRLDDLRRNISVVLQDVFLFSGTI-RXXXXXXXXXXX 404 SLING Y QKG I ID + + + L +R+ I+ V QDV LFSGTI Sbjct: 386 SLINGLYIPQKGNIFIDEIPLLEYNLKLVRKQIAAVPQDVLLFSGTILDNIRLFDESIPE 445 Query: 405 XXXXXALRASCAEAFVHSAPEGIDEPVTERGSTLSAGQRQLISFARAIAHDPAIFVLDEA 464 AL+ A + P G+ + ERG+TLSAG+RQLI+ ARA+ D IF+LDEA Sbjct: 446 ERVLEALKRVHALDIIERLPGGVYYEIVERGTTLSAGERQLIALARAVLFDAKIFILDEA 505 Query: 465 TANIDTRTEKLIQQAIDNAASGRTTLIIAHRLSTITGADLIIAMKAGRIAESGHPRELLK 524 T+N+D TE IQ+A++ + RT ++IAHRLST+ D I+ + GRI E G+ ELL+ Sbjct: 506 TSNVDVITETKIQEALEELSKDRTVIMIAHRLSTVKDVDEIVVVHNGRIVEKGNHYELLE 565 Query: 525 RGGYYAKLLQ 534 + G+Y L + Sbjct: 566 KRGFYYNLYK 575 >BS_yknV [V] COG1132 ABC-type multidrug transport system, ATPase and permease components Length = 604 Score = 304 bits (779), Expect = 2e-82, Method: Compositional matrix adjust. Identities = 183/489 (37%), Positives = 264/489 (53%), Gaps = 1/489 (0%) Query: 47 VGKAGQSLIHELRVRVFSIIERLPLPYLDRTSSGRLITRATNDTSEISDLYTDVIISLVK 106 + + GQ +I++LR +F+ ++RL + D+ S+G ++ R ND + + +L+T +I+L+ Sbjct: 108 MNQLGQHVIYDLRQHLFTHVQRLSHRFFDQRSAGSILVRIMNDINSLQELFTSGVINLLT 167 Query: 107 DVLLLAGIVYAMLVLSPKLTLVSXXXXXXXXXXXXXXXXXXXXXXXHMKHYIGQINGFIA 166 D+LLLAG++ + LSP+LT+ ++ ++N + Sbjct: 168 DLLLLAGVIIILFTLSPELTIAIMVTLPIMFFISTSLRKKIRRSWQTVRLKQSKLNSHLN 227 Query: 167 ESISGMRVIQIFRAEKEKEQQFLKLNGEYFNTTLIQVRLNSILKPASDMFQSLAIAILVW 226 ESI G+RV Q F E+E F +N E + + R N++ +P +M ++ A+L+W Sbjct: 228 ESIQGIRVTQAFTQEEENMAYFDGVNQENYESWREATRKNAMFRPLVEMTNAIGTAVLIW 287 Query: 227 YSVGRIAGGTLQIGVLYAFTTYIKQFFAPVSDLADKYTSIQSAFVSTXXXXXXXXXXXXX 286 Y I T+ IGV +F Y+ F+ P+S L Y + S+ Sbjct: 288 YGATLIMNETITIGVFVSFAFYLGMFWEPISRLGQVYNQLLMGMASSERIFEFLDEQPNV 347 Query: 287 XXPDAGLPMQRLNGRIEFRRVWFSYNGADWVLKDVSFVIEKGQTAAFIGETGAGKTTIIS 346 + +++NG I F V FSY+ L VSF I G T A +G TG+GKTTI + Sbjct: 348 KEKPNAIHNEKINGEISFEEVEFSYDEKRKALHAVSFSIPAGSTLALVGHTGSGKTTIAN 407 Query: 347 LINGFYKVQKGEILIDGVNVNDIRLDDLRRNISVVLQDVFLFSGTI-RXXXXXXXXXXXX 405 LI+ FY G I IDG+ + D+ L LR IS+VLQD F+FSGTI Sbjct: 408 LISRFYDAAGGTIKIDGIPIKDLSLASLRSQISIVLQDTFIFSGTIMENIRFGRPNASDE 467 Query: 406 XXXXALRASCAEAFVHSAPEGIDEPVTERGSTLSAGQRQLISFARAIAHDPAIFVLDEAT 465 A +A A+ F+ EG V ERGS LSAGQRQLISFARA+ DPAI +LDEAT Sbjct: 468 EVMKASQAVGADEFISDLAEGYATEVEERGSVLSAGQRQLISFARALLADPAIIILDEAT 527 Query: 466 ANIDTRTEKLIQQAIDNAASGRTTLIIAHRLSTITGADLIIAMKAGRIAESGHPRELLKR 525 A+IDT TE IQQA+ GRT ++IAHRLSTI AD II + GR E G+ +LL + Sbjct: 528 ASIDTETEVKIQQALKTLLKGRTAVMIAHRLSTIRDADRIIVLDHGRKIEEGNHDQLLAK 587 Query: 526 GGYYAKLLQ 534 GG YA L++ Sbjct: 588 GGIYAGLVK 596 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.322 0.138 0.388 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 31,748,083 Number of extensions: 1083961 Number of successful extensions: 8349 Number of sequences better than 1.0e-05: 1125 Number of HSP's gapped: 7360 Number of HSP's successfully gapped: 1188 Length of query: 543 Length of database: 38,378,431 Length adjustment: 111 Effective length of query: 432 Effective length of database: 25,576,357 Effective search space: 11048986224 Effective search space used: 11048986224 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 118 (50.1 bits)