BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0290.0030.p (341 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value NMA0246 [G] COG0057 Glyceraldehyde-3-phosphate dehydrogenase/ery... 498 e-141 NMB2159 [G] COG0057 Glyceraldehyde-3-phosphate dehydrogenase/ery... 494 e-140 L0005 [G] COG0057 Glyceraldehyde-3-phosphate dehydrogenase/eryth... 494 e-139 SA0727 [G] COG0057 Glyceraldehyde-3-phosphate dehydrogenase/eryt... 493 e-139 L0004 [G] COG0057 Glyceraldehyde-3-phosphate dehydrogenase/eryth... 461 e-130 >NMA0246 [G] COG0057 Glyceraldehyde-3-phosphate dehydrogenase/erythrose-4-phosphate dehydrogenase Length = 422 Score = 498 bits (1283), Expect = e-141, Method: Compositional matrix adjust. Identities = 28/332 (8%), Positives = 58/332 (17%), Gaps = 1/332 (0%) Query: 6 MSIKVGINGFGRIGRLAFRRIQNVEGVEVVAINDLTNAKMLAHLLKYDTTQGIFNGDIEV 65 M+ + I+ G+L E I T A +L H + G + Sbjct: 1 MTYRDFIDQLRENGKLVEVPQPVSPRFEASRIAKKTKAPVLFHDISGSKVIMNLLGSRDE 60 Query: 66 HDGYFKVNGKEVKVLMNRNPEELPWGELGVDIILECTGFLTTKEKAELHLKGGAKKVVIS 125 V +E+ ++ E + LE + + Sbjct: 61 LASMLGVPKEEIIRKLSEVSPEGEVRIVSESPTLEVIENEVDLTRLPILTHYEKDGAPYI 120 Query: 126 APATGDIKTIVYNVNHETLDGTETVISGASCTTNCLAPMAKALHDKFGIISGLMTTVHAY 185 + A+ + V Sbjct: 121 TAGIVVSEYGGVMNASIHRLMLAGKDKLAARLVPPRHTYLLHKKAVEKGEPLPVAIVIGC 180 Query: 186 TGNQNTLDAPNPKGDFRGARAAAENIVPYSTGAAKAIGLVLPELKGKLDGASQRVPVPTG 245 AAA P V V G Sbjct: 181 DPTITYATTTRVPVGKEFEYAAALRGAPVELFECSNGVKVPHSEIVLEGYVDPVEKVDEG 240 Query: 246 SVTELVSVLNTKVTVDQVNAAMKEASDSETYGYTEDEIVSSDIKGITFGSLFDATQTKVL 305 ++ + + + + + + + Sbjct: 241 PFVDITGTYDMVRKEPVIRITRVLHR-KDPIYHGILPAGPEHLLMMGVPYEPRIYRAVGE 299 Query: 306 TVQDQQLVKTVAWYDNEMSYTSQLVRTLEYFA 337 + +V T + +T Sbjct: 300 VTTVKNVVLTEGGCCYLHAVVQIKKQTEGDAK 331 >NMB2159 [G] COG0057 Glyceraldehyde-3-phosphate dehydrogenase/erythrose-4-phosphate dehydrogenase Length = 334 Score = 494 bits (1273), Expect = e-140, Method: Compositional matrix adjust. Identities = 236/332 (71%), Positives = 275/332 (82%), Gaps = 1/332 (0%) Query: 6 MSIKVGINGFGRIGRLAFRRIQNVEGVEVVAINDLTNAKMLAHLLKYDTTQGIFNGDIEV 65 MSIKV INGFGRIGRLA R+I+ +EVVA+NDLT A+ML HL KYD+TQG F G E+ Sbjct: 1 MSIKVAINGFGRIGRLALRQIEKAHDIEVVAVNDLTPAEMLLHLFKYDSTQGRFQGTAEL 60 Query: 66 HDGYFKVNGKEVKVLMNRNPEELPWGELGVDIILECTGFLTTKEKAELHLKGGAKKVVIS 125 D VNGKE+KV N NPEELPWGELGVD+ILECTGF T K KAE H++ GA+KVVIS Sbjct: 61 KDDAIVVNGKEIKVFANPNPEELPWGELGVDVILECTGFFTNKTKAEAHIRAGARKVVIS 120 Query: 126 APATGDIKTIVYNVNHETLDGTETVISGASCTTNCLAPMAKALHDKFGIISGLMTTVHAY 185 AP D+KT+VY VN + LDG+ETVIS ASCTTNCLAPMA L +FG++ GLMTT+HAY Sbjct: 121 APGGNDVKTVVYGVNQDILDGSETVISAASCTTNCLAPMAAVLQKEFGVVEGLMTTIHAY 180 Query: 186 TGNQNTLDAPNPKGDFRGARAAAENIVPYSTGAAKAIGLVLPELKGKLDGASQRVPVPTG 245 TG+QNTLDAP+ KGD R ARAAA NIVP STGAAKAIGLV+PEL GKLDG++QRVPV +G Sbjct: 181 TGDQNTLDAPHRKGDLRRARAAALNIVPNSTGAAKAIGLVIPELNGKLDGSAQRVPVASG 240 Query: 246 SVTELVSVLNTKVTVDQVNAAMKEASDSETYGYTEDEIVSSDIKGITFGSLFDATQTKVL 305 S+TELVS+L VT +++NAAMK A+ SE+YGY ED+IVSSD+ GI +GSLFDATQT+V+ Sbjct: 241 SLTELVSILERPVTKEEINAAMKAAA-SESYGYNEDQIVSSDVVGIEYGSLFDATQTRVM 299 Query: 306 TVQDQQLVKTVAWYDNEMSYTSQLVRTLEYFA 337 TV +QLVKTVAWYDNEMSYT QLVRTLEYFA Sbjct: 300 TVGGKQLVKTVAWYDNEMSYTCQLVRTLEYFA 331 >L0005 [G] COG0057 Glyceraldehyde-3-phosphate dehydrogenase/erythrose-4-phosphate dehydrogenase Length = 336 Score = 494 bits (1271), Expect = e-139, Method: Compositional matrix adjust. Identities = 245/337 (72%), Positives = 277/337 (82%), Gaps = 2/337 (0%) Query: 6 MSIKVGINGFGRIGRLAFRRIQNVEGVEVVAINDLTNAKMLAHLLKYDTTQGIFNGDIEV 65 M +KVGINGFGRIGRLAFRRIQNVEGVEVVAINDLT+ MLAHLLKYDTTQG F+G +EV Sbjct: 1 MVVKVGINGFGRIGRLAFRRIQNVEGVEVVAINDLTDPAMLAHLLKYDTTQGRFDGKVEV 60 Query: 66 HDGYFKVNGKEVKVLMNRNPEELPWGELGVDIILECTGFLTTKEKAELHLKG-GAKKVVI 124 DG F+VNGK VKV NP + W E+G +I+LE TGF TKEKAE HL GAKKVVI Sbjct: 61 KDGGFEVNGKFVKVTAESNPANINWAEVGAEIVLEATGFFATKEKAEQHLHANGAKKVVI 120 Query: 125 SAPATGDIKTIVYNVNHETLDGTETVISGASCTTNCLAPMAKALHDKFGIISGLMTTVHA 184 +AP D+KTIV+N NHE LDGTETVIS SCTTNCLAPMA L+ +FGI G MTTVH Sbjct: 121 TAPGGSDVKTIVFNTNHEVLDGTETVISAGSCTTNCLAPMADTLNKQFGIKVGTMTTVHG 180 Query: 185 YTGNQNTLDAPNPKGDFRGARAAAENIVPYSTGAAKAIGLVLPELKGKLDGASQRVPVPT 244 YTG+Q TLD P+ GDFR ARAAAENIVP STGAAKAIGLVLPEL+GKL G +QRVPVPT Sbjct: 181 YTGDQMTLDGPHRGGDFRRARAAAENIVPNSTGAAKAIGLVLPELQGKLQGHAQRVPVPT 240 Query: 245 GSVTELVSVLNTKVTVDQVNAAMKEASDSETYGYTEDEIVSSDIKGITFGSLFDATQTKV 304 GS+TELV++LN +VTVD++NAAMK AS+ E++GY ED+IVSSDI GI+ SLFDATQT+V Sbjct: 241 GSLTELVTILNKEVTVDEINAAMKAASN-ESFGYNEDQIVSSDIVGISNSSLFDATQTEV 299 Query: 305 LTVQDQQLVKTVAWYDNEMSYTSQLVRTLEYFAKIAK 341 + QLVKTV+WYDNEMSYTS LVRTL YFAKIAK Sbjct: 300 TSADGAQLVKTVSWYDNEMSYTSNLVRTLAYFAKIAK 336 >SA0727 [G] COG0057 Glyceraldehyde-3-phosphate dehydrogenase/erythrose-4-phosphate dehydrogenase Length = 336 Score = 493 bits (1268), Expect = e-139, Method: Compositional matrix adjust. Identities = 234/337 (69%), Positives = 286/337 (84%), Gaps = 2/337 (0%) Query: 6 MSIKVGINGFGRIGRLAFRRIQNVEGVEVVAINDLTNAKMLAHLLKYDTTQGIFNGDIEV 65 M++KV INGFGRIGRLAFRRIQ VEG+EVVA+NDLT+ MLAHLLKYDT QG F G++EV Sbjct: 1 MAVKVAINGFGRIGRLAFRRIQEVEGLEVVAVNDLTDDDMLAHLLKYDTMQGRFTGEVEV 60 Query: 66 HDGYFKVNGKEVKVLMNRNPEELPWGELGVDIILECTGFLTTKEKAELHLKGGAKKVVIS 125 DG F+VNGKEVK + +LPW +L +D++LECTGF T K+KA+ H++ GAKKV+IS Sbjct: 61 VDGGFRVNGKEVKSFSEPDASKLPWKDLNIDVVLECTGFYTDKDKAQAHIEAGAKKVLIS 120 Query: 126 APATGDIKTIVYNVNHETLDGTETVISGASCTTNCLAPMAKALHDKFGIISGLMTTVHAY 185 APATGD+KTIV+N NH+ LDG+ETV+SGASCTTN LAP+AK L+D FG++ GLMTT+HAY Sbjct: 121 APATGDLKTIVFNTNHQELDGSETVVSGASCTTNSLAPVAKVLNDDFGLVEGLMTTIHAY 180 Query: 186 TGNQNTLDAPNPKGDFRGARAAAENIVPYSTGAAKAIGLVLPELKGKLDGASQRVPVPTG 245 TG+QNT DAP+ KGD R ARAAAENI+P STGAAKAIG V+PE+ GKLDG +QRVPV TG Sbjct: 181 TGDQNTQDAPHRKGDKRRARAAAENIIPNSTGAAKAIGKVIPEIDGKLDGGAQRVPVATG 240 Query: 246 SVTELVSVLNTK-VTVDQVNAAMKEASDSETYGYTEDEIVSSDIKGITFGSLFDATQTKV 304 S+TEL VL + VTV+QVN AMK AS+ E++GYTEDEIVSSD+ G+T+GSLFDATQT+V Sbjct: 241 SLTELTVVLEKQDVTVEQVNEAMKNASN-ESFGYTEDEIVSSDVVGMTYGSLFDATQTRV 299 Query: 305 LTVQDQQLVKTVAWYDNEMSYTSQLVRTLEYFAKIAK 341 ++V D+QLVK AWYDNEMSYT+QLVRTL Y A+++K Sbjct: 300 MSVGDRQLVKVAAWYDNEMSYTAQLVRTLAYLAELSK 336 >L0004 [G] COG0057 Glyceraldehyde-3-phosphate dehydrogenase/erythrose-4-phosphate dehydrogenase Length = 337 Score = 461 bits (1186), Expect = e-130, Method: Compositional matrix adjust. Identities = 231/338 (68%), Positives = 268/338 (79%), Gaps = 3/338 (0%) Query: 6 MSIKVGINGFGRIGRLAFRRIQNVEGVEVVAINDLTNAKMLAHLLKYDTTQGIFNGDIEV 65 M +KVGINGFGRIGRLA RRIQ VEGVEV INDLT+ MLAHLLKYDTTQG F G +EV Sbjct: 1 MVVKVGINGFGRIGRLALRRIQEVEGVEVAHINDLTDPAMLAHLLKYDTTQGRFKGTVEV 60 Query: 66 HDGYFKVNGKEVKVLMNRNPEELPWGELGVDIILECTGFLTTKEKAELHLK-GGAKKVVI 124 + F VNGK VKV RNPE++ W + GV+I+LE TGF TKEKAE HL GGAKKV+I Sbjct: 61 KEDGFDVNGKFVKVTAERNPEDIQWADSGVEIVLEATGFFATKEKAEKHLHPGGAKKVLI 120 Query: 125 SAPATGDIKTIVYNVNHETLDGTETVISGASCTTNCLAPMAKALHDKFGIISGLMTTVHA 184 +AP D+KT+V+N NH LDGTETVIS SCTTN LAPMA AL+ FG+ G MTTVH+ Sbjct: 121 TAPGGNDVKTVVFNTNHTILDGTETVISAGSCTTNSLAPMADALNKNFGVKGGTMTTVHS 180 Query: 185 YTGNQNTLDAPNPKGDFRGARAAAENIVPYSTGAAKAIGLVLPELKGKLDGASQRVPVPT 244 YTG+Q TLD P+ GDFR ARAAAENIVP S+GAAKAIGLVLPEL G + G +QRV PT Sbjct: 181 YTGDQMTLDGPHRGGDFRRARAAAENIVPASSGAAKAIGLVLPELSGLMKGHAQRVSTPT 240 Query: 245 GSVTELVSVLNTKVTVDQVNAAMKEASDSETYGYTEDEIVSSDIKGITFGSLFDATQTKV 304 GS+TELV+VL VTVD++N AMK A+D E++GY DEIVSSDI G+ +GSLFDAT T+V Sbjct: 241 GSITELVTVLEKHVTVDEINEAMKAAAD-ESFGYNVDEIVSSDIIGMAYGSLFDATLTEV 299 Query: 305 LTVQD-QQLVKTVAWYDNEMSYTSQLVRTLEYFAKIAK 341 ++D QLVKT AWYDNEMS+T+QL+RTLEYFAKIAK Sbjct: 300 TDLKDGGQLVKTAAWYDNEMSFTAQLIRTLEYFAKIAK 337 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.315 0.132 0.373 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 24,944,978 Number of extensions: 1040978 Number of successful extensions: 1938 Number of sequences better than 1.0e-05: 52 Number of HSP's gapped: 1729 Number of HSP's successfully gapped: 52 Length of query: 341 Length of database: 38,378,431 Length adjustment: 107 Effective length of query: 234 Effective length of database: 26,037,693 Effective search space: 6092820162 Effective search space used: 6092820162 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (22.0 bits) S2: 116 (49.3 bits)