BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0290.0026.p (174 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value STM0404 [J] COG0809 S-adenosylmethionine:tRNA-ribosyltransferase... 102 3e-22 MA0137 [F] COG2131 Deoxycytidylate deaminase 101 7e-22 MA0136 [F] COG2131 Deoxycytidylate deaminase 94 9e-20 VCA0840 [F] COG2131 Deoxycytidylate deaminase 66 3e-11 VC0175 [F] COG2131 Deoxycytidylate deaminase 54 1e-07 >STM0404 [J] COG0809 S-adenosylmethionine:tRNA-ribosyltransferase-isomerase (queuine synthetase) Length = 346 Score = 102 bits (254), Expect = 3e-22, Method: Compositional matrix adjust. Identities = 9/133 (6%), Positives = 21/133 (15%), Gaps = 10/133 (7%) Query: 8 RKNWDTYFMDIACMVSTRSRCPRRHVGAVLVQGKKLLGTAYNGAPMGVPDCSEAGCMVSE 67 + + L+ K+ G + N PD + + E Sbjct: 53 YPVPPRPETQGLTETYVGNWLAKHGSREKLIIASKVSGPSRNNDKGIRPDQALDRKNIRE 112 Query: 68 QYERELVNGVETMVKKQRCIRTIHAEQNLLLFTDRSD--REGSTVYVTDEPCWTCANMLA 125 + K+ + + + S Sbjct: 113 A---LHDSL-----KRLQTDYLDLYQVHWPQRPTNCFGKLGYSWTDSAPAVSLLDTLDAL 164 Query: 126 NSGIVEIVYLRPY 138 Sbjct: 165 AEYQRAGKIRYIG 177 >MA0137 [F] COG2131 Deoxycytidylate deaminase Length = 150 Score = 101 bits (251), Expect = 7e-22, Method: Compositional matrix adjust. Identities = 53/133 (39%), Positives = 80/133 (60%), Gaps = 10/133 (7%) Query: 8 RKNWDTYFMDIACMVSTRSRCPRRHVGAVLVQGKKLLGTAYNGAPMGVPDCSEAGCMVSE 67 R + D YF++IA +V+ R+ C R +VGAV+V+ K++L T YNGAP G+ C + GC+ Sbjct: 4 RPSLDEYFLEIAFVVAKRATCLRNNVGAVIVRDKRILSTGYNGAPSGLEHCLDIGCI--- 60 Query: 68 QYERELVNGVETMVKKQRCIRTIHAEQNLLL--FTDRSDREGSTVYVTDEPCWTCANMLA 125 REL + + + ++C R +HAEQN ++ G+T+Y T +PC CA M+ Sbjct: 61 ---REL-EKIPSGTRHEKC-RAVHAEQNAIIQAAIHGVSISGATIYCTHQPCILCAKMII 115 Query: 126 NSGIVEIVYLRPY 138 NS I +VY PY Sbjct: 116 NSNIKRVVYSTPY 128 >MA0136 [F] COG2131 Deoxycytidylate deaminase Length = 150 Score = 94.4 bits (233), Expect = 9e-20, Method: Compositional matrix adjust. Identities = 50/132 (37%), Positives = 80/132 (60%), Gaps = 10/132 (7%) Query: 8 RKNWDTYFMDIACMVSTRSRCPRRHVGAVLVQGKKLLGTAYNGAPMGVPDCSEAGCMVSE 67 R + D YF++IA V+ R+ C R +VGAV+++ K++L T YNGAP G+ C E C+ Sbjct: 4 RLSLDEYFLEIAFAVARRATCLRSNVGAVIMRDKRILSTGYNGAPSGLEHCLEIECIRDL 63 Query: 68 QYERELVNGVETMVKKQRCIRTIHAEQNLLL--FTDRSDREGSTVYVTDEPCWTCANMLA 125 + ++++G + K++C R +HAEQN ++ G+T+Y T +PC CA M+ Sbjct: 64 E---KIISGTQ----KEKC-RAVHAEQNAIIQAAIHGVSIAGATIYCTQQPCTLCAKMII 115 Query: 126 NSGIVEIVYLRP 137 NS I ++VY P Sbjct: 116 NSNIKKVVYSTP 127 >VCA0840 [F] COG2131 Deoxycytidylate deaminase Length = 161 Score = 65.9 bits (159), Expect = 3e-11, Method: Compositional matrix adjust. Identities = 35/122 (28%), Positives = 61/122 (50%), Gaps = 19/122 (15%) Query: 11 WDTYFMDIACMVSTRSRCPRRHVGAVLVQGKKLLGTAYNGAPMGVPDCSEAGCMVSEQYE 70 W F +A +V + S+ P VGAV+ + +++ +NG P G+ D + + Sbjct: 5 WAQRFFQMAELVGSWSKDPSTQVGAVITKQNRIVSVGFNGYPHGISDSASTD-------D 57 Query: 71 RELVNGVETMVKKQRCIRTIHAEQNLLLFTDRSDREGSTVYVTDEPCWTCANMLANSGIV 130 R++ + ++T+HAE+N +LF R D +G +YVT PC CA + +GI Sbjct: 58 RDM-----------KYLKTLHAEENAILFAKR-DLDGCEIYVTHFPCPNCAAKIIQTGIS 105 Query: 131 EI 132 + Sbjct: 106 AV 107 >VC0175 [F] COG2131 Deoxycytidylate deaminase Length = 532 Score = 54.3 bits (129), Expect = 1e-07, Method: Composition-based stats. Identities = 43/170 (25%), Positives = 72/170 (42%), Gaps = 39/170 (22%) Query: 12 DTYFMDIACMVSTRSRCPRRHVGAVLVQGKKLL------------GTAYNGAPMGVPDCS 59 D M A + RS C R VGA ++ + + G YN + C Sbjct: 267 DEIGMHAAYSAALRSACLSRQVGAAILDNQGNIISTGCNDVPSFGGGLYNSNSLADFRCV 326 Query: 60 EAGCMVSEQYE---------------------RELVNGVETMVKKQRCI---RTIHAEQN 95 G +++++ +E+VN + + K + I R +HAE + Sbjct: 327 HTGRCSNDKHKDILKEEITDILKKSITNTLELKEIVNQITSETKIKTLIEYSRAVHAEMD 386 Query: 96 LLLFTDRSDREGS---TVYVTDEPCWTCANMLANSGIVEIVYLRPYRKDM 142 L+ R+++E S T+YVT PC CA + +GI ++VY+ PY K + Sbjct: 387 SLIALARNNKETSVDKTLYVTTYPCHNCARHIVAAGIKKVVYVEPYEKSL 436 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.321 0.134 0.403 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 11,599,319 Number of extensions: 430193 Number of successful extensions: 668 Number of sequences better than 1.0e-05: 5 Number of HSP's gapped: 661 Number of HSP's successfully gapped: 5 Length of query: 174 Length of database: 38,378,431 Length adjustment: 99 Effective length of query: 75 Effective length of database: 26,960,365 Effective search space: 2022027375 Effective search space used: 2022027375 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 112 (47.8 bits)