BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0290.0024.p (282 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value BH1331 [E] COG0345 Pyrroline-5-carboxylate reductase 201 1e-51 BS_comER [E] COG0345 Pyrroline-5-carboxylate reductase 192 5e-49 lin1424 [E] COG0345 Pyrroline-5-carboxylate reductase 103 3e-22 BS_ykeA [E] COG0345 Pyrroline-5-carboxylate reductase 96 5e-20 BS_yqjO [E] COG0345 Pyrroline-5-carboxylate reductase 86 6e-17 >BH1331 [E] COG0345 Pyrroline-5-carboxylate reductase Length = 346 Score = 201 bits (511), Expect = 1e-51, Method: Compositional matrix adjust. Identities = 16/269 (5%), Positives = 50/269 (18%), Gaps = 1/269 (0%) Query: 1 MKVGFIGTGSMGSLLIDAFLSSGALEPCDVLASNRSPNRLLQLQQRHPGISIGSN-QETA 59 M+ I S+ + + + + A + + Q + Sbjct: 1 MQYHRIPHSSLEVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGINLIDVAEMYPVPPRPE 60 Query: 60 QGSDIVFICVKPLEFKTLTDEISSSLRSEQIVVSITSPVMLHHLESALPSKIAKVIPSIT 119 L +++ + + + + + A Sbjct: 61 TQGLTETYVGNWLAKHGSREKLIIASKVSGPSRNNDKGIRPDQALDRKNIREALHDSLKR 120 Query: 120 HRVKSGTSLCIFGSRLNTKDRLLLQRLLSFMGVPIEIQEMHTRIASDFSSCGPAFISFFL 179 + + + T L + + + + +A + +I Sbjct: 121 LQTDYLDLYQVHWPQRPTNCFGKLGYSWTDSAPAVSLLDTLDALAEYQRAGKIRYIGVSN 180 Query: 180 ERWIEAAVEATGIDEELITRLAXXXXXXXXXXXXXXXFSPQELQERVAVPGGITAEALSH 239 E D+ + R+ + Q + Sbjct: 181 ETAFGVMRYLHLADKHDLPRIVTIQNPYSLLNRSFEVGLAEVSQYEGVELLAYSCLGFGT 240 Query: 240 LRTSLEGVFERLILTTHDKYDEDVEKLDA 268 L + + Sbjct: 241 LTGKYLNGAKPAGARNTLFSRFTRYSGEQ 269 >BS_comER [E] COG0345 Pyrroline-5-carboxylate reductase Length = 346 Score = 192 bits (488), Expect = 5e-49, Method: Compositional matrix adjust. Identities = 16/264 (6%), Positives = 49/264 (18%), Gaps = 1/264 (0%) Query: 1 MKVGFIGTGSMGSLLIDAFLSSGALEPCDVLASNRSPNRLLQLQQRHPGISIGSNQET-A 59 M+ I S+ + + + + A + + Q + Sbjct: 1 MQYHRIPHSSLEVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGINLIDVAEMYPVPPRPE 60 Query: 60 QGSDIVFICVKPLEFKTLTDEISSSLRSEQIVVSITSPVMLHHLESALPSKIAKVIPSIT 119 L +++ + + + + + A Sbjct: 61 TQGLTETYVGNWLAKHGSREKLIIASKVSGPSRNNDKGIRPDQALDRKNIREALHDSLKR 120 Query: 120 HRVKSGTSLCIFGSRLNTKDRLLLQRLLSFMGVPIEIQEMHTRIASDFSSCGPAFISFFL 179 + + + T L + + + + +A + +I Sbjct: 121 LQTDYLDLYQVHWPQRPTNCFGKLGYSWTDSAPAVSLLDTLDALAEYQRAGKIRYIGVSN 180 Query: 180 ERWIEAAVEATGIDEELITRLAXXXXXXXXXXXXXXXFSPQELQERVAVPGGITAEALSH 239 E D+ + R+ + Q + Sbjct: 181 ETAFGVMRYLHLADKHDLPRIVTIQNPYSLLNRSFEVGLAEVSQYEGVELLAYSCLGFGT 240 Query: 240 LRTSLEGVFERLILTTHDKYDEDV 263 L + Sbjct: 241 LTGKYLNGAKPAGARNTLFSRFTR 264 >lin1424 [E] COG0345 Pyrroline-5-carboxylate reductase Length = 346 Score = 103 bits (257), Expect = 3e-22, Method: Compositional matrix adjust. Identities = 14/270 (5%), Positives = 39/270 (14%), Gaps = 3/270 (1%) Query: 2 KVGFIGTGSMGSLLIDAFLSSGALEPCDVLASNRSPN-RLLQLQQRHPGISIGSNQ-ETA 59 + S L ++ G + + + ET Sbjct: 3 YHRIPHSSLEVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGINLIDVAEMYPVPPRPETQ 62 Query: 60 QGSDIVFICVKPLEFKTLTDEISSSLRSEQIVVSITSPVMLHHLESALPS-KIAKVIPSI 118 ++ I+S + + + Sbjct: 63 GLTETYVGNWLAKHGSREKLIIASKVSGPSRNNDKGIRPDQALDRKNIREALHDSLKRLQ 122 Query: 119 THRVKSGTSLCIFGSRLNTKDRLLLQRLLSFMGVPIEIQEMHTRIASDFSSCGPAFISFF 178 T + + ++ + + Sbjct: 123 TDYLDLYQVHWPQRPTNCFGKLGYSWTDSAPAVSLLDTLDALAEYQRAGKIRYIGVSNET 182 Query: 179 LERWIEAAVEATGIDEELITRLAXXXXXXXXXXXXXXXFSPQELQERVAVPGGITAEALS 238 + A D I + Q + + L+ Sbjct: 183 AFGVMRYLHLADKHDLPRIVTIQNPYSLLNRSFEVGLAEVSQYEGVELLAYSCLGFGTLT 242 Query: 239 HLRTSLEGVFERLILTTHDKYDEDVEKLDA 268 + E+ Sbjct: 243 GKYLNGAKPAGARNTLFSRFTRYSGEQTQK 272 >BS_ykeA [E] COG0345 Pyrroline-5-carboxylate reductase Length = 346 Score = 96.3 bits (238), Expect = 5e-20, Method: Compositional matrix adjust. Identities = 17/260 (6%), Positives = 41/260 (15%), Gaps = 1/260 (0%) Query: 2 KVGFIGTGSMGSLLIDAFLSSGALEPCDVLASNRSP-NRLLQLQQRHPGISIGSNQETAQ 60 + S L ++ G + + + Q Sbjct: 3 YHRIPHSSLEVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGINLIDVAEMYPVPPRPETQ 62 Query: 61 GSDIVFICVKPLEFKTLTDEISSSLRSEQIVVSITSPVMLHHLESALPSKIAKVIPSITH 120 G ++ + + I +S S + L+ + Sbjct: 63 GLTETYVGNWLAKHGSREKLIIASKVSGPSRNNDKGIRPDQALDRKNIREALHDSLKRLQ 122 Query: 121 RVKSGTSLCIFGSRLNTKDRLLLQRLLSFMGVPIEIQEMHTRIASDFSSCGPAFISFFLE 180 + R L + + + Sbjct: 123 TDYLDLYQVHWPQRPTNCFGKLGYSWTDSAPAVSLLDTLDALAEYQRAGKIRYIGVSNET 182 Query: 181 RWIEAAVEATGIDEELITRLAXXXXXXXXXXXXXXXFSPQELQERVAVPGGITAEALSHL 240 + +L + + E V + + Sbjct: 183 AFGVMRYLHLADKHDLPRIVTIQNPYSLLNRSFEVGLAEVSQYEGVELLAYSCLGFGTLT 242 Query: 241 RTSLEGVFERLILTTHDKYD 260 L G T Sbjct: 243 GKYLNGAKPAGARNTLFSRF 262 >BS_yqjO [E] COG0345 Pyrroline-5-carboxylate reductase Length = 346 Score = 85.9 bits (211), Expect = 6e-17, Method: Compositional matrix adjust. Identities = 17/255 (6%), Positives = 43/255 (16%), Gaps = 8/255 (3%) Query: 2 KVGFIGTGSMGSLLIDAFLSSGALEPCDVLASNRSPN-RLLQLQQRHPGISIGSNQETAQ 60 + S L ++ G + + + Q Sbjct: 3 YHRIPHSSLEVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGINLIDVAEMYPVPPRPETQ 62 Query: 61 GSDIVFICVKPLEFKTLTDEISSSLRSEQIVVSITSPVMLHHLESALPSKIA--KVIPSI 118 G ++ + + I +S + + + A + + Sbjct: 63 GLTETYVGNWLAKHGSREKLIIASKV-SGPSRNNDKGIRPDQALDRKNIREALHDSLKRL 121 Query: 119 THRVKSGTSLCIFGSRLNTKDRLLLQRLLSFMGVPIEIQEMHTRIASDFSSCGPAFISFF 178 + N +L S V + +S Sbjct: 122 QTDYLDLYQVHWPQRPTNCFGKLGYSWTDSAPAVSLLDTLDALAEYQRAGKIRYIGVSNE 181 Query: 179 LERWIEAAVEATGIDEELITRLAXXXXXXXXXXXXXXXFSPQELQERVAVPGGITAEALS 238 + +L + + E V + + Sbjct: 182 TA-FGVMRYLHLADKHDLPRIVTIQNPYSLLNRSFEVGLAEVSQYEGVELLAYSCLGFGT 240 Query: 239 HLRTSLEGVFERLIL 253 L Sbjct: 241 LTGKYL---NGAKPA 252 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.320 0.136 0.384 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 18,092,114 Number of extensions: 578071 Number of successful extensions: 1303 Number of sequences better than 1.0e-05: 17 Number of HSP's gapped: 1273 Number of HSP's successfully gapped: 17 Length of query: 282 Length of database: 38,378,431 Length adjustment: 105 Effective length of query: 177 Effective length of database: 26,268,361 Effective search space: 4649499897 Effective search space used: 4649499897 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 115 (48.9 bits)