BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0290.0018.p (355 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value BS_yrrI [R] COG0628 Predicted permease 226 5e-59 BS_yueF [R] COG0628 Predicted permease 197 3e-50 BH2921 [R] COG0628 Predicted permease 180 3e-45 lin0908 [R] COG0628 Predicted permease 175 1e-43 SA1192 [R] COG0628 Predicted permease 174 1e-43 >BS_yrrI [R] COG0628 Predicted permease Length = 353 Score = 226 bits (576), Expect = 5e-59, Method: Compositional matrix adjust. Identities = 122/337 (36%), Positives = 199/337 (59%), Gaps = 2/337 (0%) Query: 12 WMIGVLLALIILYFVWLLRPMLQGVFQFLKAILAPFLAAMIISYVLNPVVSMLAGRKMPR 71 W+ LL L+ +Y ++L + + +K I P + ++ ISY+L PV L G+ +PR Sbjct: 11 WVAICLLVLLTVYVFFMLDMLWSPFWLVIKTIFIPLIISIFISYLLLPVTEWLHGKGLPR 70 Query: 72 SVAVLLIYAVFLTSLAVILINLIPMFIEQLEELNEHLPEITLHAQGLMRNMN--TRLIPP 129 ++++L+IY +F + L +P+ I QL +L+E++P GL+ +++ T P Sbjct: 71 TLSILVIYVLFFGGIGWALYKGVPVLIVQLTDLSENIPMFAETYNGLLLHVHNHTDDWPD 130 Query: 130 GVGMGMNNWFFQLENRLAGGISHFLDNIGSTIGVLFDAFIVPFLVFYILKDFDVFERTVV 189 G+ ++ Q E AG I + I + + A +PFLVFY++KD ++ ++TV Sbjct: 131 GMHHRIDKMIRQTEAFFAGTIEGAISGIRNVLDYFLIAATIPFLVFYMVKDIELMKKTVW 190 Query: 190 SCLPRSRRKLIVKMLRDIDDALGNYIRGQFLVCIIIGVLAYIGYAIIGMPYALLFACVVA 249 P+S RK LRD+DD+LG+YIRGQ LVC+I+GV+A I + + G+PY L+ + Sbjct: 191 YLTPKSWRKRGSAFLRDVDDSLGDYIRGQLLVCLILGVIAGISFWVFGLPYPLILGLISG 250 Query: 250 VFNIVPYMGPFLGAAPAIVMXXXXXXXXXXXXXXXXXXCQMLESNVISPQVVGRKLHLHP 309 V N++PY GPF+GA PA+++ Q +E N++SP +VGR L +HP Sbjct: 251 VTNVIPYFGPFIGAVPALLIAMTISVKAVLVVVITVFILQFMEGNILSPFIVGRSLKMHP 310 Query: 310 LLIIFALLVGGEVAGVIGLILAVPVFAAGKVVIQHIM 346 ++I+ ALL GGE+AG++G+ILAVP A KV++ H + Sbjct: 311 VVIMLALLAGGELAGIVGMILAVPATAVLKVMMIHFL 347 >BS_yueF [R] COG0628 Predicted permease Length = 369 Score = 197 bits (500), Expect = 3e-50, Method: Compositional matrix adjust. Identities = 116/353 (32%), Positives = 188/353 (53%), Gaps = 31/353 (8%) Query: 6 HSKWFRWMIGVLLALIILYFVWLLRPMLQGVFQFLKAILAPFLAAMIISYVLNPVVSMLA 65 SK W + +L L+I++ + + Q F+ + P L A I+ ++ NPVV +L Sbjct: 3 KSKVHFWTLQILFVLLIIFVATKVSFVFQPFIVFISTLFFPMLIAGILYFIFNPVVRLLE 62 Query: 66 GRKMPRSVAVLLIYAVFLTSLAVILINLIPMFIEQLEELNEHLPEITLHAQGLMRNMNTR 125 +K+PR++++LLIY +F+ LA I ++ P+ Q+ L +LP+ Q L ++++ Sbjct: 63 -KKIPRTLSILLIYLLFIGLLAFISASVGPIITAQVTGLFNNLPDYIKQIQALTKDLS-- 119 Query: 126 LIPPGVGMGMNNWFF-----------QLENRLAGGISHFLDNIGSTIGVLFDA------- 167 + WF ++E L + + NI S++ +F Sbjct: 120 ---------HSQWFTWMMNQDYVSISKIEQSLTSFLQNLPQNITSSLSAVFGVVTNITLV 170 Query: 168 -FIVPFLVFYILKDFDVFERTVVSCLPRSRRKLIVKMLRDIDDALGNYIRGQFLVCIIIG 226 VPF++FY+LKD F V LP S R +K+ +D+ D L Y +GQ L+C+ +G Sbjct: 171 IITVPFILFYMLKDGHRFPHLAVKILPASYRTEGLKIFKDLSDTLAAYFQGQLLICLFVG 230 Query: 227 VLAYIGYAIIGMPYALLFACVVAVFNIVPYMGPFLGAAPAIVMXXXXXXXXXXXXXXXXX 286 +IGY I G+PYAL+ V+A+ NI+PY+GPFLGAAPA+++ Sbjct: 231 TACFIGYLIAGLPYALILGIVMAITNIIPYVGPFLGAAPAVIVGFMDSPAKALFAIIVVV 290 Query: 287 XCQMLESNVISPQVVGRKLHLHPLLIIFALLVGGEVAGVIGLILAVPVFAAGK 339 Q L+ N++SP V+G++L+ HPL II L+ G G++G+ILAVPV+A K Sbjct: 291 IVQQLDGNLLSPLVIGKRLNTHPLTIILLLIGAGSFGGILGMILAVPVYAVVK 343 >BH2921 [R] COG0628 Predicted permease Length = 361 Score = 180 bits (457), Expect = 3e-45, Method: Compositional matrix adjust. Identities = 119/369 (32%), Positives = 200/369 (54%), Gaps = 30/369 (8%) Query: 6 HSKWFRWMIGVLLALIILYF----VWLLRPMLQGVFQFLKAILAPFLAAMIISYVLNPVV 61 KWFR+ G++L L+I+Y W+ RP++ F++ + AP + A I+ Y+ P+V Sbjct: 3 QGKWFRFGYGIVLVLLIIYLGSLVDWIFRPIVV----FVQTLFAPVIIAGILYYLFRPIV 58 Query: 62 SMLAGRKMPRSVAVLLIYAVFLTSLAVILINLIPMFIEQLEELNEHLPE---------IT 112 +L+ +KM R +++L+++ + + + + + P Q + L +LP I Sbjct: 59 GLLS-KKMNRGLSILILFLMIIGAFTAFSLIVGPELQRQFQSLYNNLPTLINQVRDTFIA 117 Query: 113 LHAQGLM-RNMNTRLIPPGVGMGMNNWFFQLENRLAGGISHFLDNIGSTIGVLFDAFI-- 169 L + L R + P M + F N + G I NI IG L + FI Sbjct: 118 LQSNELFNRFQESETFQPE---EMISRFVNSLNDIIGTIGA---NITGFIGALANFFIIL 171 Query: 170 --VPFLVFYILKDFDVFERTVVSCLPRSRRKLIVKMLRDIDDALGNYIRGQFLVCIIIGV 227 VPF++FY+LK+ + ++ + ++ +L D+D L +YI+GQ +V +G+ Sbjct: 172 VLVPFILFYLLKEGEKLPVQLLKLFNKKQQHEGRLILADMDKMLSSYIQGQIIVSFCVGI 231 Query: 228 LAYIGYAIIGMPYALLFACVVAVFNIVPYMGPFLGAAPAIVMXXXXXXXXXXXXXXXXXX 287 L YI Y IIG+ YAL+ A V N++P++GP++G PA+++ Sbjct: 232 LCYIAYLIIGLDYALILALVAMFTNVIPFVGPWIGTFPAVIVAFIDSPLTALIVVIVVVV 291 Query: 288 CQMLESNVISPQVVGRKLHLHPLLIIFALLVGGEVAGVIGLILAVPVFAAGKVVIQHIMA 347 Q +ESN+ISPQV+G+KL +HP+ IIF LLV G AGV+G++LAVP +A GKV++ H Sbjct: 292 IQQIESNLISPQVMGKKLAIHPITIIFILLVAGRFAGVVGMLLAVPTYAVGKVIVTHSYR 351 Query: 348 -YYIKRKPV 355 + ++++PV Sbjct: 352 LWKLRKQPV 360 >lin0908 [R] COG0628 Predicted permease Length = 378 Score = 175 bits (443), Expect = 1e-43, Method: Compositional matrix adjust. Identities = 106/356 (29%), Positives = 184/356 (51%), Gaps = 18/356 (5%) Query: 2 EEWSHSKWFRWMIGVLLALIILYFVWLLRPMLQGVFQFLKAILAPFLAAMIISYVLNPVV 61 + SK F W I +L + +L+ + ++ + + + + P L A + Y+ NP+V Sbjct: 4 SRFRDSKLFFWTIEILAVVAVLFILLQMKYIFSPIGIIVSTLFMPILVAGFLFYLFNPLV 63 Query: 62 SMLAGRKMPRSVAVLLIYAVFLTSLAVILINLIPMFIEQLEELNEHLP----EITLHAQG 117 L RK+PR ++V+LI+ F+ + + ++ L P +Q+ EL + +P + Q Sbjct: 64 LFLEKRKVPRLLSVILIFIAFIALVVLAVMQLGPTLADQVAELAKAIPGYWQDFEKWLQS 123 Query: 118 LMRNMNTRLIPPGVGMGMNNWFFQLENRLAGGISHFLDNIGSTIGVLFD---AFI----- 169 L N + + M +L L +S +D + S+ G + F+ Sbjct: 124 LSNNSALK------DIDMKQELSKLNISLPKIMSVVVDGVASSFGAIISFVSGFVMILVT 177 Query: 170 VPFLVFYILKDFDVFERTVVSCLPRSRRKLIVKMLRDIDDALGNYIRGQFLVCIIIGVLA 229 VPF+VFY+ KD F + P R +++++++ + YI Q + C+ +G+ Sbjct: 178 VPFIVFYMFKDGHKFVESSGKFFPAGIRTEAKQIIKEMNKTISTYISSQAIDCMFVGLFT 237 Query: 230 YIGYAIIGMPYALLFACVVAVFNIVPYMGPFLGAAPAIVMXXXXXXXXXXXXXXXXXXCQ 289 +IGY IIG PYALLF + NI+PY+GPFLGAAPA+++ Q Sbjct: 238 FIGYLIIGQPYALLFGFIAGATNIIPYLGPFLGAAPAVIVALFTSPVQALLVIVVVTIVQ 297 Query: 290 MLESNVISPQVVGRKLHLHPLLIIFALLVGGEVAGVIGLILAVPVFAAGKVVIQHI 345 ++SN++SP ++G+ L +HPL II L+V G +AG+ G+IL VP++A K +I ++ Sbjct: 298 QIDSNLLSPYIMGKSLSIHPLTIIIILIVAGNLAGIFGMILGVPLYAVVKTIIVNV 353 >SA1192 [R] COG0628 Predicted permease Length = 402 Score = 174 bits (442), Expect = 1e-43, Method: Compositional matrix adjust. Identities = 104/367 (28%), Positives = 192/367 (52%), Gaps = 17/367 (4%) Query: 3 EWSHSKWFRWMIG-----VLLALIILYFVWLLRPMLQGVFQ----FLKAILAPFLAAMII 53 +++ S++ +++ G L+AL++L V + + VF K I AP + ++I+ Sbjct: 14 KFTESRYMKFVGGNDLVFSLIALVLLGIVIFIFEKVSYVFDPFIIVFKTIAAPIIVSLIL 73 Query: 54 SYVLNPVVSMLAGRKMPRSVAVLLIYAVFLTSLAVILINLIPMFIEQLEELNEHLPEITL 113 Y+ NP+V+M+ ++PR + +IY + + +I+ LIP+ Q++ L ++ P+ Sbjct: 74 FYLFNPIVNMMERYRIPRVAGISIIYLAVVGVITLIVNLLIPIIGSQVDSLVKNSPQYLE 133 Query: 114 HAQGLMRNMNTRLIPPGVGMGMNNWFFQLENRLAGGISHFLDNIGSTIGVLFDAF----- 168 + + +N+W L ++ +S F D GS I + Sbjct: 134 KLINSIDKIANNTFFSSYYSQINDWLNSLPKKIPSMLSEFTDGFGSKIATFAETIANIGV 193 Query: 169 ---IVPFLVFYILKDFDVFERTVVSCLPRSRRKLIVKMLRDIDDALGNYIRGQFLVCIII 225 PF++F++LKD F+ + +P RK +L + +G+YI+GQ +V I Sbjct: 194 VIVTTPFVLFFMLKDGHHFKEFSTNIMPPKFRKDFHDLLEKMSVQVGSYIQGQIIVSFCI 253 Query: 226 GVLAYIGYAIIGMPYALLFACVVAVFNIVPYMGPFLGAAPAIVMXXXXXXXXXXXXXXXX 285 G+L +IGY++IG+ Y+L+ A + AV ++VPY+GP + +PAIV+ Sbjct: 254 GILLFIGYSVIGLKYSLVLASIAAVTSVVPYLGPTIAISPAIVIAAITSPWMLLKLAVVW 313 Query: 286 XXCQMLESNVISPQVVGRKLHLHPLLIIFALLVGGEVAGVIGLILAVPVFAAGKVVIQHI 345 Q +E + ISP ++G+ L +HPL IIF LL G++ G++G+IL +P +A KV++ H+ Sbjct: 314 TLVQFVEGHFISPNIMGKTLKIHPLTIIFILLCAGKLLGIVGVILGIPGYAILKVLVTHL 373 Query: 346 MAYYIKR 352 + +R Sbjct: 374 FQLFKRR 380 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.333 0.148 0.456 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 25,094,305 Number of extensions: 1031966 Number of successful extensions: 4442 Number of sequences better than 1.0e-05: 95 Number of HSP's gapped: 4271 Number of HSP's successfully gapped: 95 Length of query: 355 Length of database: 38,378,431 Length adjustment: 107 Effective length of query: 248 Effective length of database: 26,037,693 Effective search space: 6457347864 Effective search space used: 6457347864 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (22.0 bits) S2: 116 (49.3 bits)