BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0290.0016.p (240 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value BH3232 [G] COG2273 Beta-glucanase/Beta-glucan synthetase 222 4e-58 BH3231 [G] COG2273 Beta-glucanase/Beta-glucan synthetase 192 4e-49 mlr5264 [G] COG2273 Beta-glucanase/Beta-glucan synthetase 134 9e-32 AGl1600 [G] COG2273 Beta-glucanase/Beta-glucan synthetase 134 1e-31 SMb20955 [G] COG2273 Beta-glucanase/Beta-glucan synthetase 132 4e-31 >BH3232 [G] COG2273 Beta-glucanase/Beta-glucan synthetase Length = 346 Score = 222 bits (565), Expect = 4e-58, Method: Compositional matrix adjust. Identities = 0/216 (0%), Positives = 0/216 (0%), Gaps = 8/216 (3%) Query: 30 TVFYEPLTYFNPVTWQKADGYSNGGMFNCTWRADNISFTGGGQLRLALT----SSSYNKF 85 Sbjct: 550 - 608 Query: 86 DGAEMRSVYKYGYGKYEVSMMPAKNSGIVSSFFTYTGPSE--GTSWDEIDIEFLGKDTTK 143 Sbjct: 609 668 Query: 144 VQFNYFTNGVGGHEKVVNLGFDASTGYHTYAFDWQPGYIKWYVDGVLKHTATSNIPSEPG 203 Sbjct: 669 728 Query: 204 KIMMNLWNGTGVDSWLGSYNGAN-PLYAYYDWLKYT 238 Sbjct: 729 764 Score = 218 bits (556), Expect = 5e-57, Method: Compositional matrix adjust. Identities = 0/216 (0%), Positives = 0/216 (0%), Gaps = 8/216 (3%) Query: 30 TVFYEPLTYFNPVTWQKADGYSNGGMFNCTWRADNISFTGGGQLRLALT----SSSYNKF 85 + T + + Sbjct: 297 RRQPFVASTLLGATTMDQLKTNIESLHLELSEDVLAEIEAV-HQVYTYP 355 Query: 86 DGAEMRSVYKYGYGKYEVSMMPAKNSGIVSSFFTYTGPSE--GTSWDEIDIEFLGKDTTK 143 Sbjct: 356 415 Query: 144 VQFNYFTNGVGGHEKVVNLGFDASTGYHTYAFDWQPGYIKWYVDGVLKHTATSNIPSEPG 203 Sbjct: 416 475 Query: 204 KIMMNLWNGTGVDSWLGSYNGAN-PLYAYYDWLKYT 238 Sbjct: 476 511 Score = 215 bits (548), Expect = 5e-56, Method: Compositional matrix adjust. Identities = 19/221 (8%), Positives = 37/221 (16%), Gaps = 13/221 (5%) Query: 30 TVFYEPLTYFNPVTWQKADGYSNGGMFNCTWRADNISFTGGGQLRLALTSSSYNKFDGAE 89 V + G+ + + + S + + Sbjct: 39 AVAQGINLIDVAEMYPVPPRPETQGLTETYVGNWLAKHGSR-EKLIIASKVSGPSRNNDK 97 Query: 90 MRSVYK---------YGYGKYEVSMMPAKNSGIVSSFFTYTGPSE--GTSWDEIDIEFLG 138 + + + + V T G SW + Sbjct: 98 GIRPDQALDRKNIREALHDSLKRLQTDYLDLYQVHWPQRPTNCFGKLGYSWTDSAPAVSL 157 Query: 139 KDTTKVQFNYFTNGVGGHEKVVNLGFDASTGYHTYAFDWQPGYIKWYVDGVLKHTATSNI 198 DT Y G + V N Y A I + + + Sbjct: 158 LDTLDALAEYQRAGKIRYIGVSNETAFGVMRYLHLADKHDLPRIVTIQNPYSLLNRSFEV 217 Query: 199 PSEPGKIMMNLWNGTGVDSWLGSYNGAN-PLYAYYDWLKYT 238 + G+ G Sbjct: 218 GLAEVSQYEGVELLAYSCLGFGTLTGKYLNGAKPAGARNTL 258 >BH3231 [G] COG2273 Beta-glucanase/Beta-glucan synthetase Length = 346 Score = 192 bits (488), Expect = 4e-49, Method: Compositional matrix adjust. Identities = 4/158 (2%), Positives = 16/158 (10%), Gaps = 2/158 (1%) Query: 84 KFDGAEMRSVYKYGYGKYEVSMMPAKNSGIVSSFFTYTGPS-EGTSWDEIDIEFLGKDTT 142 G + + + + Sbjct: 16 LGLGTMTFGEQNSEADAHAQLDYAVAQGINLIDVAEMYPVPPRPETQGLTETYVGNWLAK 75 Query: 143 KVQFNYFTNGVGGHEKVVNLGFDASTGYHTYAFDWQPGYIKWYVDGVLKHTATSNIPSEP 202 N + + + + Sbjct: 76 HGSREKLIIASKVSGPSRNNDKGIRPDQALDRKNIREALHDSLKRLQTDYLDLYQVHWPQ 135 Query: 203 GKIMMNLWNGTGVDSWLGSYNGANPLYAYYDWLKYTGN 240 G + + + + G Sbjct: 136 RPTNCFGKLGYSWTDSAPAVSLLD-TLDALAEYQRAGK 172 >mlr5264 [G] COG2273 Beta-glucanase/Beta-glucan synthetase Length = 346 Score = 134 bits (338), Expect = 9e-32, Method: Compositional matrix adjust. Identities = 16/206 (7%), Positives = 36/206 (17%), Gaps = 7/206 (3%) Query: 26 VSAATVFYEPLTYFNPVTWQKADGYSNGGMFNCTWRADNISFTGGGQLRLALTSSSY--N 83 + LT W G + + + G Sbjct: 54 PVPPRPETQGLTETYVGNWLAKHGSREKLIIASKVSGPSRNNDKG-IRPDQALDRKNIRE 112 Query: 84 KFDGAEMRSVYKYGYGKYEVSMMPAKNSGIVSSFFTYTGPSEGTSWDEIDIEFLGKDTTK 143 + R Y N + + D +D + K Sbjct: 113 ALHDSLKRLQTDYLDLYQVHWPQRPTNCFGKLGYSWTDSAPAVSLLDTLDALAEYQRAGK 172 Query: 144 VQFNYFTNGVGGHEKVVNLGFDASTGYHTYAFDWQPGYIKWYVDGVLKHTAT--SNIPSE 201 +++ +N D + + L + Sbjct: 173 IRYIGVSNETAFGVMRYLHLADKHDLPRIVTIQNPYSLLNRSFEVGLAEVSQYEGVELLA 232 Query: 202 PGKIMMNLWNGTGVDSWLGSYNGANP 227 + G ++ + A Sbjct: 233 YSCLGFGTLTGKYLNGAKPAG--ARN 256 >AGl1600 [G] COG2273 Beta-glucanase/Beta-glucan synthetase Length = 134 Score = 134 bits (338), Expect = 1e-31, Method: Compositional matrix adjust. Identities = 0/205 (0%), Positives = 0/205 (0%), Gaps = 7/205 (3%) Query: 30 TVFYEPLTYFNPVTWQKADGYSNGGMFNCTWRADNISFTGGGQLRLALTSSSYNK--FDG 87 D + + G + Sbjct: 61 VAAEGFSGIQINKALSSIDAHQETSGSGRIQ-TLRVVARQKGAAVRIDAVFNIQAGQIAD 119 Query: 88 AEMRSVYKYGYGKYEVSMMPAKNSGIVSSFFTYTGPSEGTSWDEIDIEFLGKDTTKVQFN 147 Sbjct: 120 179 Query: 148 YFTNGVGGHEKVVNLGFDASTGYHTYAFDWQPGYIKWYVDGVLKHTAT--SNIPSEPGKI 205 Sbjct: 180 239 Query: 206 MMNLWNGTGVDSWLG--SYNGANPL 228 Sbjct: 240 264 >SMb20955 [G] COG2273 Beta-glucanase/Beta-glucan synthetase Length = 346 Score = 132 bits (332), Expect = 4e-31, Method: Compositional matrix adjust. Identities = 11/212 (5%), Positives = 27/212 (12%), Gaps = 5/212 (2%) Query: 32 FYEPLTYFNPVTWQKADGYSNGGMFNCTWRADNISFTGGGQLRLALTSSSYNK--FDGAE 89 + + T R L +S + Sbjct: 38 YAVAQGINLIDVAEMYPVPPRPETQGLTETYVGNW-LAKHGSREKLIIASKVSGPSRNND 96 Query: 90 MRSVYKYGYGKYEVSMMPAKNSGIVSSFFTYTGPSEGTSWDEIDIEFLGKDTTKVQFNYF 149 + + + + + + LG T Sbjct: 97 KGIRPDQALDRKNIREALHDSLKRLQTDYLDLYQVHWPQRPTNCFGKLGYSWTDSAPAVS 156 Query: 150 TNGVGGHEKVVNLGFDASTGYHTYAFDWQPGYIKWYVDGVLKHTAT--SNIPSEPGKIMM 207 + + D N S + Sbjct: 157 LLDTLDALAEYQRAGKIRYIGVSNETAFGVMRYLHLADKHDLPRIVTIQNPYSLLNRSFE 216 Query: 208 NLWNGTGVDSWLGSYNGANPLYAYYDWLKYTG 239 + + + G Sbjct: 217 VGLAEVSQYEGVELLAYSCLGFGTLTGKYLNG 248 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.317 0.135 0.433 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 19,796,574 Number of extensions: 848002 Number of successful extensions: 1086 Number of sequences better than 1.0e-05: 10 Number of HSP's gapped: 1065 Number of HSP's successfully gapped: 12 Length of query: 240 Length of database: 38,378,431 Length adjustment: 103 Effective length of query: 137 Effective length of database: 26,499,029 Effective search space: 3630366973 Effective search space used: 3630366973 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 114 (48.5 bits)