BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0290.0015.p (513 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value BH1064 [G] COG1653 ABC-type sugar transport system, periplasmic ... 291 1e-78 BH0482 [G] COG1653 ABC-type sugar transport system, periplasmic ... 93 8e-19 BS_lplA [G] COG1653 ABC-type sugar transport system, periplasmic... 77 8e-14 BS_ytcQ [G] COG1653 ABC-type sugar transport system, periplasmic... 74 8e-13 SP1796 [G] COG1653 ABC-type sugar transport system, periplasmic ... 70 1e-11 >BH1064 [G] COG1653 ABC-type sugar transport system, periplasmic component Length = 550 Score = 291 bits (746), Expect = 1e-78, Method: Compositional matrix adjust. Identities = 182/534 (34%), Positives = 266/534 (49%), Gaps = 40/534 (7%) Query: 13 LPAASPESTAPHFDVKTGKYNPPVDLYTVGSVNPNLTFKKGESLEHNVHTIWAENRLGIR 72 L A S E + + T + + V + T +++ FK GE + NV T WAE LGI+ Sbjct: 19 LTACSQEGSTTEDETNTNE-DGTVTITTARTLDDGTVFKDGEDVHDNVVTRWAEEELGIK 77 Query: 73 IRYLWTISGTSETYANKLRLELAKGN-MPDVVTTRDANVIQELIDSGQFMEAGSLFGQFA 131 WT E + +LRL ++ G +PDV D + +L+ SG M +A Sbjct: 78 FVTEWT-QPNDEQFNTQLRLMMSSGEKLPDVFQVNDTAIKADLLSSGLVMPVDEAIETYA 136 Query: 132 SKVWKQAVAEDPSAWDSYVRDGHKYAIPIMDYEYNSDPLLWIRQDWLDKLHLSAPRTLDE 191 S KQ + P A+ +DG +Y IP SD LLWIRQDWLDK L P TL+E Sbjct: 137 SPRLKQLFEQFPQAFYPSTKDGVRYGIPRFSGGNGSDTLLWIRQDWLDKFDLEPPTTLEE 196 Query: 192 LEQVMDAFVNRDPDGNGLKDTYGLAV-----GFRNGPSTWMGDSSWIFGAFGT-VPEQWN 245 LE +MD FVN DP+ G DT G+ GFR G + DSSW+FGAFG VP W+ Sbjct: 197 LEHIMDVFVNEDPNETGKNDTIGITFAAGDYGFRGGN---IADSSWVFGAFGDFVPGAWS 253 Query: 246 RRSDGTLEYGSVQPGARKAVALMKQWVQQGYLSTDSAWLDEEGAANLFVSGKAGIIAGPY 305 + DG+L YGS+QP + +A + +W ++GYL+TD A L A F+SG++GI+A P Sbjct: 254 KGDDGSLVYGSIQPSMKDGLAKIHEWYEKGYLATDFAILSAGDAQESFISGRSGIMAAPP 313 Query: 306 WMGGWPLSHLTSADPQARIKAAAIPAGPDGTVMRRGTLPVNGAILINKKMMHPEIFFTYQ 365 W G+P+ + P A +K +GP+G RRG V G+ L NK H + FF Y Sbjct: 314 WAHGYPIGEMAQVVPDAIVKPFPHVSGPNGQAGRRGEGYVVGSFLFNKDFEHMDKFFEYW 373 Query: 366 NYLFDYYATSTGEFINGLAKDYDWTMDAGQPTIRPSAL-PLGGIRVASYTLTFDGARIPS 424 + ++ Y + F GL + YD+ M+ G+P + + G V F +P+ Sbjct: 374 DAIYGYTFGESDYFEYGLFEGYDYVMEDGEPVYDHAQFEEITGEEVVDPGRYFLPTNVPT 433 Query: 425 ------KVIKEIPK---------------------DIAPVLLSQKEASRKEQFTGPPTAT 457 +++E + + A ++ Q + +F GPPT T Sbjct: 434 IPFEMYSLLEEFHETGREPETGYEAGLAGRDPDYIEAAAIVNRQNDLRIINEFNGPPTPT 493 Query: 458 MKSDGELLKKLEQKSFQKIIFADSGIGEFDEFVDKWKAYGGLTETSEVNAWDRS 511 M S E L+++E + F II+ + + FD+FV++W+ GG T EVN W S Sbjct: 494 MVSRSEHLERMEDELFVDIIYGNKPLDAFDQFVEEWRQSGGEAITEEVNEWYES 547 >BH0482 [G] COG1653 ABC-type sugar transport system, periplasmic component Length = 512 Score = 93.2 bits (230), Expect = 8e-19, Method: Compositional matrix adjust. Identities = 113/451 (25%), Positives = 176/451 (39%), Gaps = 71/451 (15%) Query: 88 NKLRLELAKGNMPDVVTTRD--ANVIQELIDSGQFMEAGSLFGQFAS-KVWKQAVAEDPS 144 +++ LA GN+P V+T +D + + SGQF E G +F + + + E+ S Sbjct: 94 DRVNAALASGNLPKVITLQDFRTSSVLNAFRSGQFWEIGPYLDEFPNLSEMNETILENVS 153 Query: 145 AWDSYVRDGHKYAIPIMDYEYNSDPL----LWIRQDWLDKLHLSAPRTLDELEQVMDAFV 200 DG Y I Y PL + IRQDWL+ L L P L+EL +V AF Sbjct: 154 V------DGKIYGI------YRERPLSRQGVVIRQDWLENLGLEMPTNLEELYEVARAFT 201 Query: 201 NRDPDGNGLKDTYGLAVGFRNGPSTWMGDSSWIFGAFGTV------PEQWNRRSDGTLEY 254 DP+G+G DT VGF + + IFGAF T+ P +W +G L Sbjct: 202 YDDPNGSGADDT----VGFTDR-------NDLIFGAFKTLASYHGTPNEWGFDDEGKLMP 250 Query: 255 GSVQPGARKAVALMKQWVQQGYLSTDSAWLDEEGAANLFVSGKAGIIAGPYWMGGWPLSH 314 + + + MK +G ++T+ +E LF++G+AGI G Sbjct: 251 EFMTDEYVETMNYMKSLYDEGLINTEFPVTSKEQQQELFMTGRAGIYVGNMVDARNLRDK 310 Query: 315 LTSADPQARIKAAAIPAGPDGTVMRRGTLPVNGAILINKKMMHPEIFFTYQNYLFD---- 370 +P+ + AGPDG NG I K + E FD Sbjct: 311 AKDINPEMEVAITNRIAGPDGEDRVWAQGGHNGIFAIPKSSVETEAELKRILAFFDRIAE 370 Query: 371 --------------YYATSTGEFINGLAKDY-DWTMDAGQPTIRPSALPLGGIRVASYTL 415 +Y T GE+ + ++ DW + QP + A + ++ L Sbjct: 371 EDVYNLMAYGIEDVHYETIDGEYFERIEENLDDWAAEV-QPLVSLVAREMNYLKAYGDPL 429 Query: 416 TFDGARIPSKVIKEIPKDIAPVLLSQKEASRKEQFTGPPTATMKSDGELLKKLEQKSFQK 475 + R+ + I + A L S PT T + G L+K+ + K Sbjct: 430 YEEYERLTEDNNEIIVTNPAEPLFS-------------PTYTER--GTELQKIIDDATYK 474 Query: 476 IIFADSGIGEFDEFVDKWKAYGGLTETSEVN 506 I + F+ V++W GG + E+N Sbjct: 475 YILGELDEDGFEAEVERWLTNGGESIIEELN 505 >BS_lplA [G] COG1653 ABC-type sugar transport system, periplasmic component Length = 502 Score = 76.6 bits (187), Expect = 8e-14, Method: Compositional matrix adjust. Identities = 82/294 (27%), Positives = 119/294 (40%), Gaps = 38/294 (12%) Query: 66 ENRLGIRIRYLWTISGTSETYANKLRLELAKGNMPDVVTTRD--ANVIQELIDSGQFMEA 123 E G I+ W +Y +K + +A G MP + +D A I+ + +G F E Sbjct: 61 EKYTGKEIKVTWV---PDSSYNDKFNIVMASGEMPHAIVIKDKSAGFIKS-VKAGAFWEL 116 Query: 124 GSLFGQFASKVWKQAVAEDPSAWDSYVRDGHKYAIPIMDYEYNSDPL----LWIRQDWLD 179 K +K D + +G Y I Y + L + IR DWL Sbjct: 117 SPYL-----KDYKNLSQADEKILKNSSVNGEVYGI------YRTRDLIRACMIIRTDWLK 165 Query: 180 KLHLSAPRTLDELEQVMDAFVNRDPDGNGLKDTYGLAV----GFRNGPSTWMGDSSWIFG 235 + L P TLD+ +V+ AF +DPDGNG DTYG+ V G NG S W W FG Sbjct: 166 NVGLDMPETLDDFYEVLKAFKEKDPDGNGKDDTYGMVVPKWMGLGNG-SPWDVLQIW-FG 223 Query: 236 AFGTVPEQWNRRSDGTLEYGSVQPGARKAVALMKQWVQQGYLSTDSAWLDEEGAANLFVS 295 A P ++ +G L A+ K+ +G ++ D A +D + V Sbjct: 224 A----PNRYGVE-NGKLIPDFTTKEYMDALTFFKKLYDEGLINKDFAVMDSAKWNDPVVK 278 Query: 296 GKAGIIAGPYWMGGWPLSHLTSADPQAR--IKAAAIPAGPDGTVMRRGTLPVNG 347 GKAG+I S + AD + I GP+G + T P +G Sbjct: 279 GKAGVIVDTGSRASQIQSAMEEADESNKDIIDIVGSLEGPNG----KRTFPTSG 328 >BS_ytcQ [G] COG1653 ABC-type sugar transport system, periplasmic component Length = 440 Score = 73.6 bits (179), Expect = 8e-13, Method: Compositional matrix adjust. Identities = 75/260 (28%), Positives = 109/260 (41%), Gaps = 37/260 (14%) Query: 88 NKLRLELAKGNMPDVVTTRDA--NVIQELIDSGQFMEAGSLFGQFASKVWKQAVAEDPSA 145 ++L LA GN+P +VT +D + + SG F E G + + + Sbjct: 74 DRLNAALAAGNLPQIVTIQDIKNSSVMNAFRSGMFWEIGDYIKDYPNLNKMNKLINK--- 130 Query: 146 WDSYVRDGHKYAIPIMDYEYNSDPL----LWIRQDWLDKLHLSAPRTLDELEQVMDAFVN 201 + DG Y I Y PL + IR+DWLD L+L P+TLDEL +V AF Sbjct: 131 --NVTIDGKLYGI------YRERPLSRQGIVIRKDWLDNLNLKTPKTLDELYEVAKAFTE 182 Query: 202 RDPDGNGLKDTYGLAVGFRNGPSTWMGDSSWIFGAFGTV------PEQWNRRSDGTLEYG 255 DPD +G DT+GLA + I+GAF T+ P W + S G Sbjct: 183 DDPDKDGKDDTFGLA-----------DRNDLIYGAFKTIGSYEGMPTDW-KESGGKFTPD 230 Query: 256 SVQPGARKAVALMKQWVQQGYLSTDSAWLDEEGAANLFVSGKAGIIAGPYWMGGWPLSHL 315 + + + MK+ GY++ D + LF GKAGI G H Sbjct: 231 FMTQEYKDTMNYMKKLRDNGYMNKDFPVTSKTQQQELFSQGKAGIYIGNMVDAVNLRDH- 289 Query: 316 TSADPQARIKAAAIPAGPDG 335 ++D +++ GPDG Sbjct: 290 -ASDKSMKLEIINRIKGPDG 308 >SP1796 [G] COG1653 ABC-type sugar transport system, periplasmic component Length = 538 Score = 69.7 bits (169), Expect = 1e-11, Method: Compositional matrix adjust. Identities = 67/294 (22%), Positives = 127/294 (43%), Gaps = 35/294 (11%) Query: 1 LSGCAFRSTGESLPAASPESTAPHFDVKTGKYNPPVDLYTVGSVNPNLTFKKGESLEHNV 60 L+ C ++T S T P + KT K+ + N L ++ E E V Sbjct: 20 LAACGSKNTASSPDYKLEGVTFPLQEKKTLKFMTASSPLSPKDPNEKLILQRLEK-ETGV 78 Query: 61 HTIWAENRLGIRIRYLWTISGTSETYANKLRLELAKGNMPDVVTTRDANVIQEL--IDSG 118 H W + +A K L+++ G++PD + A+ + + G Sbjct: 79 HIDW---------------TNYQSDFAEKRNLDISSGDLPDAIHNDGASDVDLMNWAKKG 123 Query: 119 QFMEAGSLFGQFASKVWKQAVAEDPSAWDSYVR--DGHKYAIPIMDYE-------YNSDP 169 + L ++ + K+ + E P + + + DGH Y+ P ++ ++ + Sbjct: 124 VIIPVEDLIDKYMPNL-KKILDEKPE-YKALMTAPDGHIYSFPWIEELGDGKESIHSVND 181 Query: 170 LLWIRQDWLDKLHLSAPRTLDELEQVMDAFVNRDPDGNGLKDTYGLAVGFRNGPSTWMGD 229 + WI +DWL KL L P+T D+L +V++AF N DP+GNG D + NG D Sbjct: 182 MAWINKDWLKKLGLEMPKTTDDLIKVLEAFKNGDPNGNGEADEIPFSFISGNGNE----D 237 Query: 230 SSWIFGAFGT--VPEQWNRRSDGTLEYGSVQPGARKAVALMKQWVQQGYLSTDS 281 ++F AFG + +DG +++ + ++ V ++Q ++G + ++ Sbjct: 238 FKFLFAAFGIGDNDDHLVVGNDGKVDFTADNDNYKEGVKFIRQLQEKGLIDKEA 291 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.316 0.134 0.414 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 43,370,831 Number of extensions: 2030546 Number of successful extensions: 4515 Number of sequences better than 1.0e-05: 6 Number of HSP's gapped: 4508 Number of HSP's successfully gapped: 7 Length of query: 513 Length of database: 38,378,431 Length adjustment: 111 Effective length of query: 402 Effective length of database: 25,576,357 Effective search space: 10281695514 Effective search space used: 10281695514 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 118 (50.1 bits)