BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0290.0009.p (326 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SMb20326 [G] COG1175 ABC-type sugar transport systems, permease ... 138 1e-32 BH1245 [G] COG1175 ABC-type sugar transport systems, permease co... 134 2e-31 Cgl0704 [G] COG1175 ABC-type sugar transport systems, permease c... 133 4e-31 ZycjO [G] COG1175 ABC-type sugar transport systems, permease com... 119 5e-27 ECs1890 [G] COG1175 ABC-type sugar transport systems, permease c... 119 5e-27 >SMb20326 [G] COG1175 ABC-type sugar transport systems, permease components Length = 328 Score = 138 bits (347), Expect = 1e-32, Method: Compositional matrix adjust. Identities = 87/257 (33%), Positives = 146/257 (56%), Gaps = 16/257 (6%) Query: 23 KRVRDNKFAYMLMVPTIIFMMLVHLLPMLQGIWMSFLKLNQFTLVKYLKAPFVGLDNYVG 82 +RVR A++ + PT + + LV P+++ I+ SF +L A FVG NY+ Sbjct: 26 QRVRS---AWLFLAPTFLVLALVAGWPLIRTIYFSF---TNASLTNLSGAEFVGFANYLS 79 Query: 83 -LLFNSDNPVRQGLNF------AIRNTAIYAVIVTIGVMIMGLMMAMLLNRDFPGRGIAR 135 + S + +GL A+ NT + V+ +GL++A++LN FPGRG+ R Sbjct: 80 WITLKSGRTIYRGLLADPAWWNAVWNTLKFTVLSVSIETALGLIVALVLNAQFPGRGLVR 139 Query: 136 TAMLLPWVVPSYVVGVLWGFMW-QQNGIINYILVDVLHLFAEKPFWLLGTNT-IWAIIIP 193 A+L+PW +P+ V +W +M Q GI+N +L+ L L EK W +T + A +I Sbjct: 140 AAILIPWAIPTIVSAKMWAWMLNDQFGILNDMLIG-LGLIGEKIAWTASPDTAMIAELIV 198 Query: 194 TIWRGWPFLMVIFLAGLQTIPDDIYEAATIDGAGKFRQFWNMTLPMLKPVIAVQLLFQII 253 +W+ PF+ ++ LAGLQ +P DIYEAA IDG R FW +TLP+++P + V ++F+++ Sbjct: 199 DVWKTTPFMALLILAGLQMVPGDIYEAAKIDGVHPVRVFWRVTLPLIRPALMVAVIFRML 258 Query: 254 NNVYSYNIVSTMFGNGA 270 + + ++++ + N A Sbjct: 259 DALRIFDLIYVLTPNNA 275 >BH1245 [G] COG1175 ABC-type sugar transport systems, permease components Length = 445 Score = 134 bits (337), Expect = 2e-31, Method: Compositional matrix adjust. Identities = 81/291 (27%), Positives = 152/291 (52%), Gaps = 11/291 (3%) Query: 7 NNLPAKAPSRKRGLWRKRVRDNKFAYMLMVPTIIFMMLVHLLPMLQGIWMSFLKLNQFTL 66 N P + S K + + + A + V + ++ ++ +F Q Sbjct: 129 NYEPVRDQSLKYAKLDRDIAEGYQAELSEVLATVEPLITEADQEHAELYQNFYHYVQNIE 188 Query: 67 VKYLKAPFVGLDNYVGLLFNSDNPVRQGLNFAIRNTAIYAVIVTIGVMIMGLMMAMLLNR 126 L++ F+G +YV + +S + A+ NT + V+ +++GL +A+L+NR Sbjct: 189 TAILESNFIGFSHYVNYVQDSR------MWAAMSNTLFFTVVSVAAELVLGLAIALLINR 242 Query: 127 DFPGRGIARTAMLLPWVVPSYVVGVLWGFMWQ-QNGIINYILVDVLHLFAEKPFWL-LGT 184 F GRG+ R A+L+PW +P+ V ++W +++ Q G+I + + L L ++ L G Sbjct: 243 TFKGRGLVRVAVLIPWAIPTAVAAMMWRYLYDGQFGVIAHYF-EQLGLISDSSVLLSTGA 301 Query: 185 NTIWAIIIPTIWRGWPFLMVIFLAGLQTIPDDIYEAATIDGAGKFRQFWNMTLPMLKPVI 244 +++II +W+ P++ ++ LAGLQTIP+ +YEAA +DGA + + F+ +TLP+LK I Sbjct: 302 GAMFSIIFADVWKTTPYMALLLLAGLQTIPNSLYEAAKVDGANRIQTFFKITLPLLKSAI 361 Query: 245 AVQLLFQIINNVYSYNIVSTMFGNGAGYPGEWGDLLM--TALTRQSFGYWS 293 V LLF+ ++ ++++ + G G E + T ++Q+FG S Sbjct: 362 LVALLFRTLDAFRVFDLIFVLTGGGPANSTESISIYAYRTLFSQQNFGAGS 412 >Cgl0704 [G] COG1175 ABC-type sugar transport systems, permease components Length = 344 Score = 133 bits (334), Expect = 4e-31, Method: Compositional matrix adjust. Identities = 83/267 (31%), Positives = 143/267 (53%), Gaps = 30/267 (11%) Query: 26 RDNKFAYMLMVPTIIFMMLVHLLPMLQGIWMSFLKLNQF--TLVKYLKAPFVGLDNYV-- 81 + + A L+ P ++ + +V P+++ IW+SF T + F GLDNY+ Sbjct: 5 KQARSAAWLIAPALVVLAVVIGYPIVRAIWLSFQADKGLDPTTGLFTDGGFAGLDNYLYW 64 Query: 82 --GLLFNSDNPVRQ------GLNF--AIRNTAIYAVIVTIGV-MIMGLMMAMLLNRDFPG 130 SD +R +F A+R T ++ +VT+G+ I+G MA+++N++F G Sbjct: 65 LTQRCMGSDGTIRTCPPGTLATDFWPALRIT-LFFTVVTVGLETILGTAMALIMNKEFRG 123 Query: 131 RGIARTAMLLPWVVPSYVVGVLWGFMWQQNGIINYILVDVLHLFAEKPFWLLGTNTIWA- 189 R + R A+L+PW +P+ V LW F++ GIIN +F W T WA Sbjct: 124 RALVRAAILIPWAIPTAVTAKLWQFIFAPQGIIN-------SMFGLSVSW---TTDPWAA 173 Query: 190 ---IIIPTIWRGWPFLMVIFLAGLQTIPDDIYEAATIDGAGKFRQFWNMTLPMLKPVIAV 246 +I+ +W+ PF+ ++ LAGLQ IP + YEAA +DGA ++QF +TLP+++P + V Sbjct: 174 RAAVILADVWKTTPFMALLILAGLQMIPKETYEAARVDGATAWQQFTKITLPLVRPALMV 233 Query: 247 QLLFQIINNVYSYNIVSTMFGNGAGYP 273 +LF+ ++ + Y++ M + + P Sbjct: 234 AVLFRTLDALRMYDLPVIMISSSSNSP 260 >ZycjO [G] COG1175 ABC-type sugar transport systems, permease components Length = 293 Score = 119 bits (299), Expect = 5e-27, Method: Compositional matrix adjust. Identities = 75/207 (36%), Positives = 120/207 (57%), Gaps = 11/207 (5%) Query: 49 PMLQGIWMSFLKLNQFTLVKYLKAPFVGLDNYVGLLFNSDNPVRQGLNFAIRNTAIYAVI 108 PM+ I +SFL+L L +++ FVG+ NYV +L SD G ++ T Y + Sbjct: 32 PMVSNIEISFLRL---PLNPNIESTFVGVSNYVRIL--SD----PGFWHSLWMTVWYTAL 82 Query: 109 VTIGVMIMGLMMAMLLNRDFPGRGIARTAMLLPWVVPSYVVGVLWGFMWQQN-GIINYIL 167 V G ++GL +AM NR+F R AR+ ++L +V PS + W +M+ GI+NY+ Sbjct: 83 VVAGSTVLGLAVAMFFNREFRLRKTARSLVILSYVTPSISLVFAWKYMFNNGYGIVNYLG 142 Query: 168 VDVLHLFAEKPFWLLGTNTIWA-IIIPTIWRGWPFLMVIFLAGLQTIPDDIYEAATIDGA 226 VD+LHL+ + P W + + +++ IWR +P+ + FLA LQTI +YEAA +DGA Sbjct: 143 VDLLHLYEQAPLWFDNPGSSFVLVVLFAIWRYFPYAFISFLAILQTIDKSLYEAAEMDGA 202 Query: 227 GKFRQFWNMTLPMLKPVIAVQLLFQII 253 +++F +TLP + PV+A + + I Sbjct: 203 NAWQRFRIVTLPAIMPVLATVVTLRTI 229 >ECs1890 [G] COG1175 ABC-type sugar transport systems, permease components Length = 293 Score = 119 bits (299), Expect = 5e-27, Method: Compositional matrix adjust. Identities = 76/207 (36%), Positives = 120/207 (57%), Gaps = 11/207 (5%) Query: 49 PMLQGIWMSFLKLNQFTLVKYLKAPFVGLDNYVGLLFNSDNPVRQGLNFAIRNTAIYAVI 108 PM+ I +SFL+L L +++ FVG+ NYV +L SD G ++ T Y + Sbjct: 32 PMVSNIEISFLRL---PLNPNIESTFVGVSNYVRIL--SD----PGFWHSLWMTVWYTAL 82 Query: 109 VTIGVMIMGLMMAMLLNRDFPGRGIARTAMLLPWVVPSYVVGVLWGFMWQQN-GIINYIL 167 V G ++GL +AM NR+F R AR+ ++L +V PS + W +M+ GI+NY+ Sbjct: 83 VVAGSTVLGLAVAMFFNREFRLRKTARSLVILSYVTPSISLVFAWKYMFNNGYGIVNYLG 142 Query: 168 VDVLHLFAEKPFWLLGTNTIWA-IIIPTIWRGWPFLMVIFLAGLQTIPDDIYEAATIDGA 226 VD+LHL+ + P W + + ++I IWR +P+ + FLA LQTI +YEAA +DGA Sbjct: 143 VDLLHLYEQAPLWFDNPGSSFVLVVIFAIWRYFPYAFISFLAILQTIDKSLYEAAEMDGA 202 Query: 227 GKFRQFWNMTLPMLKPVIAVQLLFQII 253 +++F +TLP + PV+A + + I Sbjct: 203 NAWQRFRIVTLPAIMPVLATVVTLRTI 229 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.330 0.143 0.472 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 23,588,209 Number of extensions: 976749 Number of successful extensions: 3498 Number of sequences better than 1.0e-05: 234 Number of HSP's gapped: 3254 Number of HSP's successfully gapped: 244 Length of query: 326 Length of database: 38,378,431 Length adjustment: 107 Effective length of query: 219 Effective length of database: 26,037,693 Effective search space: 5702254767 Effective search space used: 5702254767 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.8 bits) S2: 116 (49.3 bits)