BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0290.0004.p (381 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value BH1260 [E] COG1104 Cysteine sulfinate desulfinase/cysteine desul... 384 e-106 lin1548 [E] COG1104 Cysteine sulfinate desulfinase/cysteine desu... 337 1e-92 MA3264 [E] COG1104 Cysteine sulfinate desulfinase/cysteine desul... 327 3e-89 BS_yrvO [E] COG1104 Cysteine sulfinate desulfinase/cysteine desu... 322 8e-88 MA2718 [E] COG1104 Cysteine sulfinate desulfinase/cysteine desul... 303 3e-82 >BH1260 [E] COG1104 Cysteine sulfinate desulfinase/cysteine desulfurase and related enzymes Length = 386 Score = 384 bits (986), Expect = e-106, Method: Compositional matrix adjust. Identities = 190/381 (49%), Positives = 254/381 (66%), Gaps = 3/381 (0%) Query: 1 MKPIYLDHAASTPVHPEVAKVMYNMLLEQFGNASSVHTYGRSAKKMINSARDTIAGFLGC 60 M+PIYLDHAA++PVHPEV + M EQFGN SS+H +GR A++ ++ AR TIA L Sbjct: 1 MEPIYLDHAATSPVHPEVIQAMLPYYEEQFGNPSSIHQFGRRARQGVDEARGTIARLLQA 60 Query: 61 APDEWVFTSGGTESDNLALFGAASASAHKGKHIITTAVEHHAVLHTCEALEKQGFSVTYL 120 P E++FTSGGTE+DNLA+FG A KG HIIT+ VEHHAVLH C+ LE QGF VTY+ Sbjct: 61 DPSEFIFTSGGTEADNLAIFGYAYQHRGKGNHIITSQVEHHAVLHACQELEHQGFEVTYV 120 Query: 121 PVNTEGRVSAADVEAALREDTVLISVMFANNEVGTVEPIQEIGRLAAERGILFHVDAVQV 180 PV+ GRVS DV ALR+DT+L+++M+ NNEVGT++PI EIG L + + H DAVQ Sbjct: 121 PVDQTGRVSVEDVRQALRDDTILVTLMYGNNEVGTIQPIAEIGALLQDHQAVLHTDAVQA 180 Query: 181 LGLLSIALRELPVDYMSFTAHKINGPQGIGGLYVRRGAPLQPRQHGGLQERGRRAGTESL 240 G +SI L LPVD +S +AHKINGP+G+G LYVR G L+P +GG QER +RAGTE++ Sbjct: 181 FGAISIELDHLPVDMLSVSAHKINGPKGVGLLYVRDGIVLKPALYGGEQERKKRAGTENV 240 Query: 241 AGAAGFAKAVELAVTGLEQRREKALTLRTVLLQELDRRIGRESYVINGDKQHFLPGILNL 300 A GFAKAVE+A+ E+R++ + + +V+NG + LP I N+ Sbjct: 241 AAIIGFAKAVEIAIANREERQKAYFDYCQTFFDQFQQE--GVQFVMNGHQTWRLPHIFNV 298 Query: 301 SFPGVSTDVMLMNLDMEXXXXXXXXXXXXXXLEISHVLRAMKLPD-ELLNSAIRFSTGMG 359 SFPGV + +L+NLD+ +E SHVL AM D EL+ S +RFS G+G Sbjct: 299 SFPGVHVEALLVNLDLAGIAASSGSACTAGSIEPSHVLVAMHGSDSELVTSGVRFSFGLG 358 Query: 360 NTNEEMEYVAQKVETILKRLR 380 NT E +++ A++ I+KRL+ Sbjct: 359 NTKEHVQWAAKETAKIVKRLQ 379 >lin1548 [E] COG1104 Cysteine sulfinate desulfinase/cysteine desulfurase and related enzymes Length = 148 Score = 337 bits (865), Expect = 1e-92, Method: Compositional matrix adjust. Identities = 0/379 (0%), Positives = 0/379 (0%), Gaps = 3/379 (0%) Query: 4 IYLDHAASTPVHPEVAKVMYNMLLEQFGNASSVHTYGRSAKKMINSARDTIAGFLGCAPD 63 A V K++ + + S+ Y R T+ L D Sbjct: 5 NTALKKAGLKVTLPRLKILEVLQEPDNHHVSAEDLYKRLIDMGEEIGLATVYRVLNQFDD 64 Query: 64 EWVFTSGGTESDNLALFGAASASAHKGKHIITTAVEHHAVLHTCEALEKQGFSVTYLPVN 123 + T E + V + + N Sbjct: 65 AGIVTRHNFEGGKSVFELTQQHHHDHLICLDCGKVIEFSDDSIEARQREIAAKHGIRLTN 124 Query: 124 TEGRVSAADVEAALREDTVLISVMFANNEVGTVEPIQEIGRLAAERGILFHVDAVQVLGL 183 Sbjct: 125 184 Query: 184 LSIALRELPVDYMSFTAHKINGPQGIGGLYVRRGAPLQPRQHGGLQERGRRAGTESLAGA 243 Sbjct: 185 244 Query: 244 AGFAKAVELAVTGLEQRREKALTLRTVLLQELDRRIGRESYVINGDKQHFLPGILNLSFP 303 Sbjct: 245 -- 302 Query: 304 GVSTDVMLMNLDMEXXXXXXXXXXXXXXLEISHVLRAMKLPDE-LLNSAIRFSTGMGNTN 362 Sbjct: 303 362 Query: 363 EEMEYVAQKVETILKRLRK 381 Sbjct: 363 381 >MA3264 [E] COG1104 Cysteine sulfinate desulfinase/cysteine desulfurase and related enzymes Length = 448 Score = 327 bits (837), Expect = 3e-89, Method: Compositional matrix adjust. Identities = 175/381 (45%), Positives = 240/381 (62%), Gaps = 8/381 (2%) Query: 4 IYLDHAASTPVHPEVAKVMYNMLLEQFGNASSVHTYGRSAKKMINSARDTIAGFLGCA-P 62 IY+DHAA+T PEV + M L E FGN SS+++ GR K+ + ++R +A LG A P Sbjct: 58 IYMDHAATTFTKPEVIEAMLPFLKEHFGNPSSLYSIGREGKEAVETSRKKLAKALGAAQP 117 Query: 63 DEWVFTSGGTESDNLALFGAASASAHKGKHIITTAVEHHAVLHTCEALEKQGFSVTYLPV 122 +E FTSGGTESDN A+ G A + KGKHIITT +EHHAVL+ CE LE QGF VTYLPV Sbjct: 118 EEIYFTSGGTESDNWAIKGTAFSRQKKGKHIITTPIEHHAVLYPCEYLETQGFEVTYLPV 177 Query: 123 NTEGRVSAADVEAALREDTVLISVMFANNEVGTVEPIQEIGRLAAERGILFHVDAVQVLG 182 + G V+ A+VEA++++DTVLISVM+ANNE+GT+EPI EIG++A E GI FH DAVQV+G Sbjct: 178 DEYGLVNPAEVEASIKKDTVLISVMYANNEIGTIEPILEIGKIAREHGIPFHTDAVQVIG 237 Query: 183 LLSIALR--ELPVDYMSFTAHKINGPQGIGGLYVRRGAPLQPRQHGGLQERGRRAGTESL 240 + + L+ VD +S ++HK GP+GIG LY+R G + HGG QERG+RAGTE++ Sbjct: 238 KVPLDLQREHKDVDMLSLSSHKFYGPKGIGALYIREGTEIDNYMHGGAQERGKRAGTENV 297 Query: 241 AGAAGFAKAVELAVTGLEQRREKALTLRTVLLQE-LDRRIGRESYVINGDKQHFLPGILN 299 G G KA+ELA +E EK +R L+ L+ R +NG + LPG LN Sbjct: 298 VGIVGMGKAIELATANIEAHNEKLSKMRARLMAGILEIPYCR----LNGHPEKRLPGNLN 353 Query: 300 LSFPGVSTDVMLMNLDMEXXXXXXXXXXXXXXLEISHVLRAMKLPDELLNSAIRFSTGMG 359 SF + + +L+ LD E SHVLRA+ L + +R + G Sbjct: 354 FSFEYIEGESLLLMLDQMGICCSTGSACSSGSSEPSHVLRAIGLAPKTAQGTLRLTLGDA 413 Query: 360 NTNEEMEYVAQKVETILKRLR 380 N+ E+++YV + + ++ +LR Sbjct: 414 NSEEDIDYVLEVLPEVVGKLR 434 >BS_yrvO [E] COG1104 Cysteine sulfinate desulfinase/cysteine desulfurase and related enzymes Length = 346 Score = 322 bits (824), Expect = 8e-88, Method: Compositional matrix adjust. Identities = 161/341 (47%), Positives = 218/341 (63%), Gaps = 2/341 (0%) Query: 1 MKPIYLDHAASTPVHPEVAKVMYNMLLEQFGNASSVHTYGRSAKKMINSARDTIAGFLGC 60 M+ IYLDHAA++P+ V + M FGN SS+H++GR ++K ++ AR IA +G Sbjct: 1 MERIYLDHAATSPMDERVLEQMIPHFSGSFGNPSSIHSFGRESRKWVDEARAQIAAEIGA 60 Query: 61 APDEWVFTSGGTESDNLALFGAASASAHKGKHIITTAVEHHAVLHTCEALEKQGFSVTYL 120 A E +FTSGGTE+DNLA+ G A A G+HIITT +EHHAVLHTCE LE GF +TYL Sbjct: 61 AEQEIIFTSGGTEADNLAIMGTALARKDLGRHIITTKIEHHAVLHTCEKLEGDGFDITYL 120 Query: 121 PVNTEGRVSAADVEAALREDTVLISVMFANNEVGTVEPIQEIGRLAAERGILFHVDAVQV 180 V+ GRVSA V+ ALR+DT+L++VM+ NNEVGTV+PI+EIG L E FH DAVQ Sbjct: 121 DVDQNGRVSAKQVKEALRDDTILVTVMYGNNEVGTVQPIEEIGELLKEHKAYFHTDAVQA 180 Query: 181 LGLLSIALRELPVDYMSFTAHKINGPQGIGGLYVRRGAPLQPRQHGGLQERGRRAGTESL 240 GLL I ++ +D +S + HK+NGP+G G LY + L P GG QER RRAGTE++ Sbjct: 181 FGLLPIDVKNSHIDLLSVSGHKLNGPKGTGFLYASKDVKLSPLLFGGEQERKRRAGTENV 240 Query: 241 AGAAGFAKAVELAVTGLEQRREKALTLRTVLLQELDRRIGRESYVINGDKQHFLPGILNL 300 G G +A++L+ +++ EK + + + L R ++ +NGDK+H LP +LNL Sbjct: 241 PGIVGLKEAIKLSSEERDEKNEKYQSFKAIFADTL--RDAGVAFEVNGDKEHSLPHVLNL 298 Query: 301 SFPGVSTDVMLMNLDMEXXXXXXXXXXXXXXLEISHVLRAM 341 FPGVS + +L+NLDM + SHVL AM Sbjct: 299 YFPGVSVEALLVNLDMAGVAVSSGSACTAGSVLPSHVLTAM 339 >MA2718 [E] COG1104 Cysteine sulfinate desulfinase/cysteine desulfurase and related enzymes Length = 392 Score = 303 bits (776), Expect = 3e-82, Method: Compositional matrix adjust. Identities = 153/379 (40%), Positives = 239/379 (63%), Gaps = 3/379 (0%) Query: 2 KPIYLDHAASTPVHPEVAKVMYNMLLEQFGNASSVHTYGRSAKKMINSARDTIAGFLGCA 61 + +Y+D++A+TPV EV + M L E FGN SS++ G+ +K + +AR +A +G Sbjct: 4 RTVYMDNSATTPVRKEVVEEMLPYLTENFGNPSSIYELGKISKHAVENARKRVADAIGAE 63 Query: 62 PDEWVFTSGGTESDNLALFGAASASAHKGKHIITTAVEHHAVLHTCEALEKQGFSVTYLP 121 +E FTSGGTESDN + G A A+ ++GKHIIT+++EHHAVLH C LE QGF VTYLP Sbjct: 64 ENEIYFTSGGTESDNWTVKGVAFANKNRGKHIITSSIEHHAVLHACAWLEGQGFEVTYLP 123 Query: 122 VNTEGRVSAADVEAALREDTVLISVMFANNEVGTVEPIQEIGRLAAERGILFHVDAVQVL 181 V+ G VS +++ A+R+DT+LIS+M ANNE+GT++P++EIG+++ E I FH DAVQ + Sbjct: 124 VDRYGMVSPEELKNAIRDDTILISIMLANNEIGTIQPVEEIGKISRENSIYFHTDAVQAI 183 Query: 182 GLLSIALRELPVDYMSFTAHKINGPQGIGGLYVRRGAPLQPRQHGGLQERGRRAGTESLA 241 G + I ++++ VD +S + HK GP+G G LY+R+G ++ HGG QER RRAGTE++ Sbjct: 184 GHVPIDVKKMNVDLLSLSGHKFGGPKGCGALYIRKGTKIEAFLHGGAQERKRRAGTENVP 243 Query: 242 GAAGFAKAVELAVTGLEQRREKALTLRTVLLQELDRRIGRESYVINGDKQHFLPGILNLS 301 G KA+ LA +E+ + L +R L++ L + +NG L +N++ Sbjct: 244 SIVGLGKAIGLATGEMEETNKPLLEMRERLIKGL---LQIPKTHLNGHPTERLANNVNVT 300 Query: 302 FPGVSTDVMLMNLDMEXXXXXXXXXXXXXXLEISHVLRAMKLPDELLNSAIRFSTGMGNT 361 F + + +L+ L+ + LE SHVL A +P E+++ ++R S G NT Sbjct: 301 FEYIEGESLLLLLNAKGIFASTGSACNSTSLEPSHVLTACGVPHEIVHGSLRLSLGRMNT 360 Query: 362 NEEMEYVAQKVETILKRLR 380 E+++ V + + I+++LR Sbjct: 361 LEDVDRVLEVLPEIVQKLR 379 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.318 0.134 0.378 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 25,908,744 Number of extensions: 936947 Number of successful extensions: 2182 Number of sequences better than 1.0e-05: 103 Number of HSP's gapped: 1996 Number of HSP's successfully gapped: 103 Length of query: 381 Length of database: 38,378,431 Length adjustment: 108 Effective length of query: 273 Effective length of database: 25,922,359 Effective search space: 7076804007 Effective search space used: 7076804007 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 117 (49.7 bits)