BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0290.0003.p (159 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value PA0312 [S] COG1683 Uncharacterized conserved protein 119 2e-27 >PA0312 [S] COG1683 Uncharacterized conserved protein Length = 164 Score = 119 bits (297), Expect = 2e-27, Method: Compositional matrix adjust. Identities = 66/147 (44%), Positives = 89/147 (60%), Gaps = 1/147 (0%) Query: 2 IIVSSCLAGMKVRYNS-TDCLDETIHQLLDNNRAVAVCPELLGEFSTPREPAEIVGGDGG 60 ++VS CL G VRY+ + + + + R VA+CPE+ G TPR PAEI GG GG Sbjct: 4 VLVSRCLLGHLVRYDGGSHGPFDLLSRWHAQGRIVALCPEVAGGLPTPRPPAEIPGGQGG 63 Query: 61 AVLDGTAKVMDRLGNDVSEMYILGAYKALEQARNAGASIVVLKENSPSCGSSMIYNGAFA 120 VLD +V G DVSE ++LGA A+E R + VLK SPSCG+ Y+G F Sbjct: 64 EVLDRLNRVFTVDGEDVSEAFLLGAEAAVELVRRHDIRMAVLKARSPSCGNLENYDGRFN 123 Query: 121 GQKIPGEGVTAALLRRNGIEVISEEQL 147 G ++ GEGVTAA L+R G+ V +E++L Sbjct: 124 GNRVAGEGVTAAALKRMGVLVFNEDEL 150 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.315 0.135 0.380 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 11,854,453 Number of extensions: 502788 Number of successful extensions: 1060 Number of sequences better than 1.0e-05: 1 Number of HSP's gapped: 1059 Number of HSP's successfully gapped: 1 Length of query: 159 Length of database: 38,378,431 Length adjustment: 98 Effective length of query: 61 Effective length of database: 27,075,699 Effective search space: 1651617639 Effective search space used: 1651617639 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.5 bits) S2: 111 (47.4 bits)