BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0284.0010.p (185 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value lin0219 [G] COG0395 ABC-type sugar transport system, permease co... 154 8e-38 CAC0428 [G] COG0395 ABC-type sugar transport system, permease co... 139 2e-33 TM0598 [G] COG0395 ABC-type sugar transport system, permease com... 134 1e-31 STM0598 [IQR] COG1028 Dehydrogenases with different specificitie... 134 1e-31 BS_araQ [G] COG0395 ABC-type sugar transport system, permease co... 131 6e-31 >lin0219 [G] COG0395 ABC-type sugar transport system, permease component Length = 282 Score = 154 bits (389), Expect = 8e-38, Method: Compositional matrix adjust. Identities = 80/184 (43%), Positives = 112/184 (60%) Query: 1 MAAYGFSKIIFKGRDVLFLLVLATFMIPTQAILVPQFIMYRWLGLFDSHIXXXXXXXXXX 60 MAAY F++I F GRD LFLL LAT M+P Q ++PQFI+ + G DS+ Sbjct: 98 MAAYAFARIPFWGRDKLFLLYLATMMVPAQVTMIPQFILMKQFGWLDSYAGLIVPALFSV 157 Query: 61 XXTFLLRQFFLGIHDEIIESARIDAAGHWTIFSRIALPLVQPALATYMILRFIWTWNDYQ 120 TFLLRQ F+GI E+ E+A +D A H+TIF ++ LPL +P AT IL F+ +WN Y Sbjct: 158 FGTFLLRQAFMGIPKELEEAAFMDGANHFTIFRKVILPLAKPTFATLGILTFMQSWNSYL 217 Query: 121 NPLVFLRSDSLFTLQLGIRKFADFSGEFYSLMMAGAVSAILPLLIIFIIGQKQVIEGVAM 180 PL+ S + TL LG+ G Y LMMAG + +++P+L +++ QK I+G+AM Sbjct: 218 WPLIVTSSQEMATLPLGLSLLQGRYGTNYGLMMAGVLISVIPILAVYLFAQKYFIQGMAM 277 Query: 181 GSVK 184 +K Sbjct: 278 SGMK 281 >CAC0428 [G] COG0395 ABC-type sugar transport system, permease component Length = 273 Score = 139 bits (351), Expect = 2e-33, Method: Compositional matrix adjust. Identities = 70/185 (37%), Positives = 113/185 (61%) Query: 1 MAAYGFSKIIFKGRDVLFLLVLATFMIPTQAILVPQFIMYRWLGLFDSHIXXXXXXXXXX 60 MAA+ FS + FKG+ +LF+ VLAT MIP + L+ ++ LG DS+ Sbjct: 89 MAAFAFSFLDFKGKKILFIAVLATLMIPAETTLISNYLTVGSLGWLDSYKALIVPYMCSA 148 Query: 61 XXTFLLRQFFLGIHDEIIESARIDAAGHWTIFSRIALPLVQPALATYMILRFIWTWNDYQ 120 F++RQ++L I E+ E+A+ID G + F++I +P+ +P +A++ I F+ +WN Y Sbjct: 149 MGIFMMRQYYLTIPKELYEAAKIDGCGSFGFFTKILMPVSRPVIASFGIYSFLVSWNQYL 208 Query: 121 NPLVFLRSDSLFTLQLGIRKFADFSGEFYSLMMAGAVSAILPLLIIFIIGQKQVIEGVAM 180 PL+ +S T+Q+GI + + L+MAG V +LP ++IFIIGQKQ+++G+ Sbjct: 209 WPLLVTSKESSRTVQIGISMLQFSENQSFGLIMAGIVIVLLPSIVIFIIGQKQLVDGMTS 268 Query: 181 GSVKG 185 G+VKG Sbjct: 269 GAVKG 273 >TM0598 [G] COG0395 ABC-type sugar transport system, permease component Length = 277 Score = 134 bits (336), Expect = 1e-31, Method: Compositional matrix adjust. Identities = 72/184 (39%), Positives = 109/184 (59%) Query: 1 MAAYGFSKIIFKGRDVLFLLVLATFMIPTQAILVPQFIMYRWLGLFDSHIXXXXXXXXXX 60 +AAY FS ++F GRD +F L LAT MIP + +LVP FI LG D++ Sbjct: 94 LAAYAFSWMVFPGRDFIFGLFLATMMIPGEVLLVPNFITISKLGWIDTYYALIIPWIVSV 153 Query: 61 XXTFLLRQFFLGIHDEIIESARIDAAGHWTIFSRIALPLVQPALATYMILRFIWTWNDYQ 120 FLLRQ FL I E+ ++A+ID HW ++ +PL +PA+ T +L+F+ +WN + Sbjct: 154 FAIFLLRQHFLTIPRELFDAAKIDGCSHWRFLWQMVVPLSKPAVITSALLKFVGSWNAFL 213 Query: 121 NPLVFLRSDSLFTLQLGIRKFADFSGEFYSLMMAGAVSAILPLLIIFIIGQKQVIEGVAM 180 L+ S+ TL +G++ F+ G Y+L+MA A +ILP++I+FI QK I+G+A Sbjct: 214 WVLIVTNSEKYRTLPVGLQAFSSDVGTQYNLLMAAATFSILPVVILFIFTQKYFIQGIAR 273 Query: 181 GSVK 184 +K Sbjct: 274 TGLK 277 >STM0598 [IQR] COG1028 Dehydrogenases with different specificities (related to short-chain alcohol dehydrogenases) Length = 251 Score = 134 bits (336), Expect = 1e-31, Method: Compositional matrix adjust. Identities = 0/184 (0%), Positives = 0/184 (0%) Query: 1 MAAYGFSKIIFKGRDVLFLLVLATFMIPTQAILVPQFIMYRWLGLFDSHIXXXXXXXXXX 60 ++ + + F L T + + Sbjct: 94 LSVDDWQQTFAVNVGGAFNLFSQTMAQFRRQQGGAIVTVASDAAHTPRIGMSAYGASKAA 153 Query: 61 XXTFLLRQFFLGIHDEIIESARIDAAGHWTIFSRIALPLVQPALATYMILRFIWTWNDYQ 120 + L + + + + + + Sbjct: 154 LKSLALTVGLELAGCGVRCNVVSPGSTDTDMQRTLWVSEDAEQQRIRGFGEQFKLGIPLG 213 Query: 121 NPLVFLRSDSLFTLQLGIRKFADFSGEFYSLMMAGAVSAILPLLIIFIIGQKQVIEGVAM 180 Sbjct: 214 273 Query: 181 GSVK 184 Sbjct: 274 277 >BS_araQ [G] COG0395 ABC-type sugar transport system, permease component Length = 281 Score = 131 bits (329), Expect = 6e-31, Method: Compositional matrix adjust. Identities = 68/185 (36%), Positives = 110/185 (59%) Query: 1 MAAYGFSKIIFKGRDVLFLLVLATFMIPTQAILVPQFIMYRWLGLFDSHIXXXXXXXXXX 60 M YG + FKGR+++F+LVL M+P + +++P F + L L DS+ Sbjct: 97 MIGYGLAVYDFKGRNIIFVLVLIIMMVPLEVMMLPLFKLTVGLHLIDSYTGVILPLIASP 156 Query: 61 XXTFLLRQFFLGIHDEIIESARIDAAGHWTIFSRIALPLVQPALATYMILRFIWTWNDYQ 120 F RQ+ LG+ ++++SAR+D + IF RI PL++PA +IL+ + +WN++ Sbjct: 157 VAVFFFRQYALGLPRDLLDSARMDGCTEFGIFFRIMAPLMKPAFGAMIILQSLNSWNNFL 216 Query: 121 NPLVFLRSDSLFTLQLGIRKFADFSGEFYSLMMAGAVSAILPLLIIFIIGQKQVIEGVAM 180 PL+ LRS +FTL +G+ G Y ++++G+V AILP++IIF+ QK I G+ + Sbjct: 217 WPLIVLRSKEMFTLPIGLSSLLSPYGNNYDMLISGSVFAILPVIIIFLFFQKYFISGLTV 276 Query: 181 GSVKG 185 G VKG Sbjct: 277 GGVKG 281 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.334 0.147 0.452 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 11,796,153 Number of extensions: 406871 Number of successful extensions: 1736 Number of sequences better than 1.0e-05: 145 Number of HSP's gapped: 1573 Number of HSP's successfully gapped: 145 Length of query: 185 Length of database: 38,378,431 Length adjustment: 100 Effective length of query: 85 Effective length of database: 26,845,031 Effective search space: 2281827635 Effective search space used: 2281827635 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 39 (21.6 bits) S2: 112 (47.8 bits)