BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0281.0004.p (334 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value BH0413 [G] COG0366 Glycosidases 281 7e-76 YPO4080 [G] COG0366 Glycosidases 225 8e-59 ZmalS [G] COG0366 Glycosidases 220 3e-57 malS [G] COG0366 Glycosidases 220 3e-57 BS_malS [C] COG0281 Malic enzyme 220 3e-57 >BH0413 [G] COG0366 Glycosidases Length = 958 Score = 281 bits (720), Expect = 7e-76, Method: Compositional matrix adjust. Identities = 143/243 (58%), Positives = 176/243 (72%), Gaps = 9/243 (3%) Query: 90 SSGGQQQEASPFSWDNANVYFVMTDRFNNGNTSNDGAYGRPKTDAWGSNVGTFHGGDIKG 149 S GG+QQ FSWDNA VYF +TDRF++GN SN+ +YGRP+ DAWG N+GTFHGGD++G Sbjct: 36 SQGGEQQS---FSWDNATVYFAITDRFHDGNPSNNQSYGRPQEDAWGQNIGTFHGGDLQG 92 Query: 150 LTQKLEEGYFTDLGTNAIWITAPWEQMHGWV-GGKDGDFAHYGYHGYYALDFTAMDKSMG 208 LT KL EGYFT+LG NAIWITAP+EQ+HGWV GG DGDFAHY YHGYYALD+T +D++MG Sbjct: 93 LTDKLNEGYFTELGINAIWITAPYEQVHGWVGGGSDGDFAHYAYHGYYALDYTMIDQNMG 152 Query: 209 TIAEMREFVDLAHAKGIRVVLDVVMNHVAYPTLVDMAEYGYGDRGGLAGSWIPDKGQGQN 268 T+ +MREFV AH +GIRVVLDVVMNH Y T+ DM EYG+G+ G++ W P GQGQN Sbjct: 153 TVEDMREFVHTAHEQGIRVVLDVVMNHPGYNTIKDMHEYGFGNP-GVSEHWTP--GQGQN 209 Query: 269 WHTHNNIMNTNNXXXXXXXXXXXXIRANNIAGYDQCGGSDQTLCVGFLPDIKTESTSGXX 328 WH + +N + IRA IAGY+ CG S+ T+C+ LPD +TE T Sbjct: 210 WHDVHQQINYED-AGAWRHWWGPWIRA-GIAGYESCGNSEITMCLAGLPDFRTELTHSVG 267 Query: 329 XSP 331 P Sbjct: 268 LPP 270 >YPO4080 [G] COG0366 Glycosidases Length = 687 Score = 225 bits (574), Expect = 8e-59, Method: Compositional matrix adjust. Identities = 121/244 (49%), Positives = 152/244 (62%), Gaps = 19/244 (7%) Query: 93 GQQQEASPFSWDNANVYFVMTDRFNNGNTSNDGAYGRPKTDAWGSNVGTFHGGDIKGLTQ 152 Q Q+A+PFSW NA VYF +TDRFNNGN +ND +YGR +GTFHGGD+ GLT+ Sbjct: 178 AQTQQAAPFSWQNATVYFALTDRFNNGNPANDHSYGRHGDGM--QEIGTFHGGDLAGLTE 235 Query: 153 KLEEGYFTDLGTNAIWITAPWEQMHGWVGG-KDGDFAHYGYHGYYALDFTAMDKSMGTIA 211 KL+ Y LG NA+WI++P EQ+HGWVGG GDF HY YHGYY LD+T +D +MGT Sbjct: 236 KLD--YLQQLGVNALWISSPLEQIHGWVGGGTKGDFPHYAYHGYYGLDWTRLDANMGTEQ 293 Query: 212 EMREFVDLAHAKGIRVVLDVVMNHVAYPTLVDM------AEYGYGDR-----GGLAGSWI 260 ++R V+ AH +GIR++ DVVMNHV Y TL DM A Y GD+ G W Sbjct: 294 DLRTLVEQAHKRGIRILFDVVMNHVGYATLADMQNDQFGALYLQGDQQEKTLGKRWSDWT 353 Query: 261 PDKGQGQNWHTHNNIMNTNNXXXXXXXXXXXXIRANNIAGYDQCGGSDQTLCVGFLPDIK 320 P G GQ WH+ N+ +N ++ IR +I YD G D T+ + FLPDIK Sbjct: 354 P--GSGQTWHSFNDYINFSDKTAWDNWWGKKWIR-TDIGDYDTPGYDDLTMSLAFLPDIK 410 Query: 321 TEST 324 TEST Sbjct: 411 TEST 414 >ZmalS [G] COG0366 Glycosidases Length = 676 Score = 220 bits (560), Expect = 3e-57, Method: Compositional matrix adjust. Identities = 116/241 (48%), Positives = 148/241 (61%), Gaps = 19/241 (7%) Query: 98 ASPFSWDNANVYFVMTDRFNNGNTSNDGAYGRPKTDAWGSNVGTFHGGDIKGLTQKLEEG 157 ++PF W NA VYFV+TDRF NG+ SND +YGR K + +GTFHGGD++GLT KL+ Sbjct: 181 SAPFDWHNATVYFVLTDRFENGDPSNDQSYGRHKDGM--AEIGTFHGGDLRGLTNKLD-- 236 Query: 158 YFTDLGTNAIWITAPWEQMHGWVGG-KDGDFAHYGYHGYYALDFTAMDKSMGTIAEMREF 216 Y LG NA+WI+AP+EQ+HGWVGG GDF HY YHGYY D+T +D +MG A++R Sbjct: 237 YLQQLGVNALWISAPFEQIHGWVGGGTKGDFPHYAYHGYYTQDWTNLDANMGNEADLRTL 296 Query: 217 VDLAHAKGIRVVLDVVMNHVAYPTLVDMAEYGYG-----------DRGGLAGSWIPDKGQ 265 VD AH +GIR++ DVVMNH Y TL DM EY +G G W P Sbjct: 297 VDSAHQRGIRILFDVVMNHTGYATLADMQEYQFGALYLSGDEVKKTLGERWSDWKP--AA 354 Query: 266 GQNWHTHNNIMNTNNXXXXXXXXXXXXIRANNIAGYDQCGGSDQTLCVGFLPDIKTESTS 325 GQ WH+ N+ +N ++ IR +I YD G D T+ + FLPDIKTEST+ Sbjct: 355 GQTWHSFNDYINFSDKTGWDKWWGKNWIR-TDIGDYDNPGFDDLTMSLAFLPDIKTESTT 413 Query: 326 G 326 Sbjct: 414 A 414 >malS [G] COG0366 Glycosidases Length = 676 Score = 220 bits (560), Expect = 3e-57, Method: Compositional matrix adjust. Identities = 116/241 (48%), Positives = 148/241 (61%), Gaps = 19/241 (7%) Query: 98 ASPFSWDNANVYFVMTDRFNNGNTSNDGAYGRPKTDAWGSNVGTFHGGDIKGLTQKLEEG 157 ++PF W NA VYFV+TDRF NG+ SND +YGR K + +GTFHGGD++GLT KL+ Sbjct: 181 SAPFDWHNATVYFVLTDRFENGDPSNDQSYGRHKDGM--AEIGTFHGGDLRGLTNKLD-- 236 Query: 158 YFTDLGTNAIWITAPWEQMHGWVGG-KDGDFAHYGYHGYYALDFTAMDKSMGTIAEMREF 216 Y LG NA+WI+AP+EQ+HGWVGG GDF HY YHGYY D+T +D +MG A++R Sbjct: 237 YLQQLGVNALWISAPFEQIHGWVGGGTKGDFPHYAYHGYYTQDWTNLDANMGNEADLRTL 296 Query: 217 VDLAHAKGIRVVLDVVMNHVAYPTLVDMAEYGYG-----------DRGGLAGSWIPDKGQ 265 VD AH +GIR++ DVVMNH Y TL DM EY +G G W P Sbjct: 297 VDSAHQRGIRILFDVVMNHTGYATLADMQEYQFGALYLSGDEVKKSLGERWSDWKP--AA 354 Query: 266 GQNWHTHNNIMNTNNXXXXXXXXXXXXIRANNIAGYDQCGGSDQTLCVGFLPDIKTESTS 325 GQ WH+ N+ +N ++ IR +I YD G D T+ + FLPDIKTEST+ Sbjct: 355 GQTWHSFNDYINFSDKTGWDKWWGKNWIR-TDIGDYDNPGFDDLTMSLAFLPDIKTESTT 413 Query: 326 G 326 Sbjct: 414 A 414 >BS_malS [C] COG0281 Malic enzyme Length = 566 Score = 220 bits (560), Expect = 3e-57, Method: Compositional matrix adjust. Identities = 9/241 (3%), Positives = 29/241 (12%), Gaps = 19/241 (7%) Query: 98 ASPFSWDNANVYFVMTDRFNNGNTSNDGAYGRPKTDAWGSNVGTFHGGDIKGLTQKLEEG 157 + + N + + + D Sbjct: 181 VYTAAAGIDPSRVLAVVLDAGTNQESLLNDPLYVGNQ--HSRVRGERYDQFIDDYVAL-- 236 Query: 158 YFTDLGTNAIWITAPWEQMHGWVGG-KDGDFAHYGYHGYYALDFTAMDKSMGTIAEMREF 216 + + + + + A Sbjct: 237 ARETFPNALLHWEDFGAKNARSILKRYKDKVCTFNDDIQGTGAVSLAAVLSCAKASKVPL 296 Query: 217 VDLAHAKGIRVVLDVVMNHVAYPTLVDMAEYGYG-----------DRGGLAGSWIPDKGQ 265 D + + LV + Sbjct: 297 RDHRVVIFGAGTAGIGIAEQLREALVREGLSEEESYKRFWCIDRNGLLTDDMDQLL--DF 354 Query: 266 GQNWHTHNNIMNTNNXXXXXXXXXXXXIRANNIAGYDQCGGSDQTLCVGFLPDIKTESTS 325 + + + + + G S + + S Sbjct: 355 QKPYARSADEVKDYQRNGDGGGIDLLEVV-RQAKPTILIGTSTVSGAFTEEIVKEMASHV 413 Query: 326 G 326 Sbjct: 414 K 414 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.319 0.135 0.432 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 24,269,152 Number of extensions: 959042 Number of successful extensions: 2813 Number of sequences better than 1.0e-05: 54 Number of HSP's gapped: 2766 Number of HSP's successfully gapped: 54 Length of query: 334 Length of database: 38,378,431 Length adjustment: 107 Effective length of query: 227 Effective length of database: 26,037,693 Effective search space: 5910556311 Effective search space used: 5910556311 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 116 (49.3 bits)