BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0280.0029.p (411 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value BH2400 [E] COG0527 Aspartokinases 419 e-117 BS_dapG [E] COG0527 Aspartokinases 412 e-115 lin1475 [E] COG0527 Aspartokinases 384 e-106 CAC1810 [E] COG0527 Aspartokinases 319 5e-87 TM1518 [E] COG0527 Aspartokinases 221 2e-57 >BH2400 [E] COG0527 Aspartokinases Length = 411 Score = 419 bits (1078), Expect = e-117, Method: Compositional matrix adjust. Identities = 214/409 (52%), Positives = 286/409 (69%), Gaps = 10/409 (2%) Query: 6 MGILVQKFGGTSLSTPAAREHVIRHVKRELASGFSLVVVVSAMGRKGEPYATDTLLDWAV 65 M I++QKFGGTS+ T RE +HV++ + G+ +VVVVSAMGR G+PYATDTLL Sbjct: 1 MKIIIQKFGGTSVKTAEVREMAAKHVEKAVEGGYKVVVVVSAMGRSGDPYATDTLLQLV- 59 Query: 66 QNGDALPSREKDLLMCCGEIISATTLCGLLEHEGVPATVLTGAQAGFLTDNNYGNARILD 125 + + RE+DLL+ CGEIIS+ LL +G+ AT +TGAQAGF T+ + NA+I+D Sbjct: 60 -DTKTIAKREQDLLVSCGEIISSVVFTELLLKKGIQATAMTGAQAGFRTNEEFTNAKIVD 118 Query: 126 VRTERILRELREHKVVIVTGFQGQTEAGDFTTLGRGGSDTSATALGAALRADMVDIYTDV 185 ++ +R+ + L VV+V GFQGQ+ G+ TTLGRGGSDTSATALGAAL A+ VDI+TDV Sbjct: 119 MKVDRLKKTLVHQDVVVVAGFQGQSPTGETTTLGRGGSDTSATALGAALDAEWVDIFTDV 178 Query: 186 DGILTADPRIVEDAKQLSYVSYTEICNMAYQGAKVIHPRAVEIAMQAQIPVRVRSTFSEA 245 +GI+TADPRIV +A+ L ++Y E+CNMAYQGAKVIHPRAVEIAMQA+IP+ +RST+S+ Sbjct: 179 EGIMTADPRIVSEARPLDTMTYNEVCNMAYQGAKVIHPRAVEIAMQAKIPIHIRSTYSDG 238 Query: 246 EGTLVTH---PEGFSDVSHGVVDRFVTGIAYVSNVTQISVECPEGNGTGVQLQIFKSMAD 302 +GTLVT G DV DR +TGIA++S VTQI V +G +Q ++F++MA Sbjct: 239 KGTLVTSMLTEAGARDVQ----DRIITGIAHMSGVTQIKVHAKDGE-FDLQEKVFRAMAA 293 Query: 303 NGISVDFINVTPTEALYTVNDDKSEQAIAALQELGLRPSSLSGCAKXXXXXXXXXXXXXX 362 ISVDFIN+ T +YTV D+ +++AI L+ELG P CAK Sbjct: 294 EKISVDFININLTGVVYTVMDEVADRAIEVLRELGFDPEVKRHCAKVSAVGAGMTGVPGV 353 Query: 363 MARIVEALSSQNIQILQSADSNTTIWVLVKKEDMVQSLRALHAKFELHR 411 A+IV ALS +NI+ILQSADS+TTIWVLV ++D+ +++ ALH F L + Sbjct: 354 TAKIVAALSQENIRILQSADSHTTIWVLVSEDDLTKAVNALHRMFHLEQ 402 >BS_dapG [E] COG0527 Aspartokinases Length = 404 Score = 412 bits (1060), Expect = e-115, Method: Compositional matrix adjust. Identities = 215/406 (52%), Positives = 283/406 (69%), Gaps = 2/406 (0%) Query: 6 MGILVQKFGGTSLSTPAAREHVIRHVKRELASGFSLVVVVSAMGRKGEPYATDTLLDWAV 65 M I+VQKFGGTS+ R+ + H+K ++ G+ +VVVVSAMGRKG+PYATD+LL Sbjct: 1 MKIIVQKFGGTSVKDDKGRKLALGHIKEAISEGYKVVVVVSAMGRKGDPYATDSLLGLLY 60 Query: 66 QNGDALPSREKDLLMCCGEIISATTLCGLLEHEGVPATVLTGAQAGFLTDNNYGNARILD 125 + A+ RE+DLL+ CGE IS+ +L GV A LTGAQAGFLT++ + NA+I++ Sbjct: 61 GDQSAISPREQDLLLSCGETISSVVFTSMLLDNGVKAAALTGAQAGFLTNDQHTNAKIIE 120 Query: 126 VRTERILRELREHKVVIVTGFQGQTEAGDFTTLGRGGSDTSATALGAALRADMVDIYTDV 185 ++ ER+ L H V+V GFQG TE GD TT+GRGGSDTSA ALGAA+ A+ +DI+TDV Sbjct: 121 MKPERLFSVLANHDAVVVAGFQGATEKGDTTTIGRGGSDTSAAALGAAVDAEYIDIFTDV 180 Query: 186 DGILTADPRIVEDAKQLSYVSYTEICNMAYQGAKVIHPRAVEIAMQAQIPVRVRSTFSEA 245 +G++TADPR+VE+AK L V+YTEICN+AYQGAKVI PRAVEIAMQA++P+RVRST+S Sbjct: 181 EGVMTADPRVVENAKPLPVVTYTEICNLAYQGAKVISPRAVEIAMQAKVPIRVRSTYSND 240 Query: 246 EGTLVTHPEGFSDVSHGVVDRFVTGIAYVSNVTQISVECPEGNGTGVQLQIFKSMADNGI 305 +GTLVT S V V +R +TGIA+V +VTQ V G VQ ++FK+MA+ GI Sbjct: 241 KGTLVTSHHS-SKVGSDVFERLITGIAHVKDVTQFKVPAKIGQ-YNVQTEVFKAMANAGI 298 Query: 306 SVDFINVTPTEALYTVNDDKSEQAIAALQELGLRPSSLSGCAKXXXXXXXXXXXXXXMAR 365 SVDF N+TP+E +YTV +K+E A L ++G P CAK ++ Sbjct: 299 SVDFFNITPSEIVYTVAGNKTETAQRILMDMGYDPMVTRNCAKVSAVGAGIMGVPGVTSK 358 Query: 366 IVEALSSQNIQILQSADSNTTIWVLVKKEDMVQSLRALHAKFELHR 411 IV ALS + I ILQSADS+TTIWVLV + DMV ++ ALH +FEL + Sbjct: 359 IVSALSEKEIPILQSADSHTTIWVLVHEADMVPAVNALHEEFELSK 404 >lin1475 [E] COG0527 Aspartokinases Length = 403 Score = 384 bits (985), Expect = e-106, Method: Compositional matrix adjust. Identities = 203/405 (50%), Positives = 267/405 (65%), Gaps = 6/405 (1%) Query: 6 MGILVQKFGGTSLSTPAAREHVIRHVKRELASGFSLVVVVSAMGRKGEPYATDTLLDWAV 65 M I+VQKFGGTS+ R H+K L G+ +VVVVSA+GR G+PYATDTLL+ Sbjct: 1 MKIIVQKFGGTSVQNEKIRLMAFNHIKNALKDGYKVVVVVSAIGRYGDPYATDTLLELIG 60 Query: 66 QNGDALPSREKDLLMCCGEIISATTLCGLLEHEGVPATVLTGAQAGFLTDNNYGNARILD 125 L +RE+D L+ GE ISA+ +L + A +G QAG +T NN+ NA+I + Sbjct: 61 AKDTKLTAREQDTLLSVGETISASVFTNMLREADIKAEAFSGGQAGIITSNNHLNAKITE 120 Query: 126 VRTERILRELREHKVVIVTGFQGQTEAGDFTTLGRGGSDTSATALGAALRADMVDIYTDV 185 V T+R+ + L V +V GFQG TE GD TTLGRGGSDTSA ALG +L+AD +DI+TDV Sbjct: 121 VDTKRLKKALETLDVAVVAGFQGITENGDITTLGRGGSDTSAAALGVSLQADYIDIFTDV 180 Query: 186 DGILTADPRIVEDAKQLSYVSYTEICNMAYQGAKVIHPRAVEIAMQAQIPVRVRSTFSEA 245 DG++TADPRIVE A+ L VSY E+ NMAYQGAKVIHPRAVEIAM A++P+R+RST+ E+ Sbjct: 181 DGMMTADPRIVEHARSLPRVSYNEVSNMAYQGAKVIHPRAVEIAMTAKVPMRIRSTYLES 240 Query: 246 EGTLVTHPEGFSDVSH-GVVDRFVTGIAYVSNVTQISVECPEGNGTGVQLQIFKSMADNG 304 GTLVT D H V +R VTG+A+V+N+TQI V+ + Q Q FK +AD G Sbjct: 241 TGTLVTSLA--DDSGHFDVKERMVTGVAHVTNLTQIFVKT---DTVKAQQQTFKILADAG 295 Query: 305 ISVDFINVTPTEALYTVNDDKSEQAIAALQELGLRPSSLSGCAKXXXXXXXXXXXXXXMA 364 IS+DFIN++ ++TV ++K L+E L+ + CAK A Sbjct: 296 ISLDFINISTDSVIFTVPEEKRRVVKQLLEEANLQTTVREDCAKVSIVGAGITGVPGVTA 355 Query: 365 RIVEALSSQNIQILQSADSNTTIWVLVKKEDMVQSLRALHAKFEL 409 +IV ALS +NI ILQSADS+TTIWVLV++ED++ ++ ALH F L Sbjct: 356 KIVGALSEKNIPILQSADSHTTIWVLVREEDLISAVNALHDVFCL 400 >CAC1810 [E] COG0527 Aspartokinases Length = 399 Score = 319 bits (818), Expect = 5e-87, Method: Compositional matrix adjust. Identities = 167/405 (41%), Positives = 247/405 (60%), Gaps = 8/405 (1%) Query: 6 MGILVQKFGGTSLSTPAAREHVIRHVKRELASGFSLVVVVSAMGRKGEPYATDTLLDWAV 65 M I+VQKFGGTS+STP R V++ V + G+S +VVVSAMGR GEPYATDTLL Sbjct: 1 MKIIVQKFGGTSVSTPERRSFVVQKVSSAIKEGYSPIVVVSAMGRAGEPYATDTLLSLID 60 Query: 66 QNGDALPSREKDLLMCCGEIISATTLCGLLEHEGVPATVLTGAQAGFLTDNNYGNARILD 125 + R DLLMCCGEIIS + L+ + A +TG QAG +TD+ Y NA + Sbjct: 61 NDFKETNKRASDLLMCCGEIISTVIMSNELKRAKIAAIPVTGGQAGIITDDEYSNASVKK 120 Query: 126 VRTERILRELREHKVVIVTGFQGQTEAGDFTTLGRGGSDTSATALGAALRADMVDIYTDV 185 V E +L+ L ++V +V GFQG+++ G FTTLGRGGSD +A+ +G A+ A+ ++IYTDV Sbjct: 121 VNPENLLKLLCHNEVPVVAGFQGKSQNGFFTTLGRGGSDVTASLIGCAVNAEKIEIYTDV 180 Query: 186 DGILTADPRIVEDAKQLSYVSYTEICNMAYQGAKVIHPRAVEIAMQAQIPVRVRSTFSEA 245 DGI+TADPRIV+DA + +SY E+ A QGAKVIHPRAVE+AM IP+ +++T ++ Sbjct: 181 DGIMTADPRIVQDAALIETISYNEVFEFAEQGAKVIHPRAVEVAMNGNIPLIIKNTLNDC 240 Query: 246 EGTLVTHPEGFSDVSHGVVDRFVTGIAYVSNVTQISVECPEGNGTGVQLQIFKSMADNGI 305 +GTL+ EG S + +TGI +S Q++ + + + + + I Sbjct: 241 KGTLINR-EGDS-----LNKNLITGITSLSGRVQVTTKFKDSKIKSTEF--LSLLGERNI 292 Query: 306 SVDFINVTPTEALYTVNDDKSEQAIAALQELGLRPSSLSGCAKXXXXXXXXXXXXXXMAR 365 ++D INV P E ++T++ + L + ++ C+K MA+ Sbjct: 293 NIDLINVFPKENIFTIDKKDLNKLKDTLIKTKVQYYLEDDCSKIAIIGNRICGVPGVMAK 352 Query: 366 IVEALSSQNIQILQSADSNTTIWVLVKKEDMVQSLRALHAKFELH 410 I++A++ + I++LQ+ADS+TTIW LVK +++ ALH +F L+ Sbjct: 353 ILKAITKEGIEVLQTADSHTTIWCLVKSNVRNKAINALHREFNLN 397 >TM1518 [E] COG0527 Aspartokinases Length = 401 Score = 221 bits (562), Expect = 2e-57, Method: Compositional matrix adjust. Identities = 147/422 (34%), Positives = 223/422 (52%), Gaps = 39/422 (9%) Query: 6 MGILVQKFGGTSLSTPAAREHVIRHVKRELASGFSLVVVVSAMGRKGEPYATDTLLDWAV 65 M ++VQK+GG+S++TP ++V + +K+++ G+ +VVVVSAMG+ TD L+ A Sbjct: 1 MNVVVQKYGGSSVATPERIKNVAQRIKKKVDEGYKVVVVVSAMGK-----TTDNLIKLAK 55 Query: 66 QNGDALPSREKDLLMCCGEIISATTLCGLLEHEGVPATVLTGAQAGFLTDNNYGNARILD 125 + SRE D+L+ GE +SA L L+ GV A L Q T ++ +ARI+D Sbjct: 56 EISPRPDSRELDMLLATGEQVSAALLSMALKDLGVKAKSLNAFQVKIKTTPHHTSARIVD 115 Query: 126 VRTERILRELREHKVVIVTGFQGQTEAGDFTTLGRGGSDTSATALGAALRADMVDIYTDV 185 + I L+++ V++VTGFQG E GD TTLGRGGSDTSA AL A LR +IY+DV Sbjct: 116 IDDSVIRENLKDYDVLVVTGFQGVNEHGDLTTLGRGGSDTSAVALAAKLRVP-CEIYSDV 174 Query: 186 DGILTADPRIVEDAKQLSYVSYTEICNMAYQGAKVIHPRAVEIAMQAQIPVRVRSTFSEA 245 DGI T DPR+ AK+L+Y++Y E + GAKV+H R+VEIA + IP+ S+F+E Sbjct: 175 DGIYTCDPRVHPRAKKLAYITYDEALELTALGAKVLHSRSVEIAKKYGIPIYCASSFTEE 234 Query: 246 EGTLVTH--PEGFSD-------VSHGVVDRFVTGIAYVSNVTQISVECPEGNGTGVQLQI 296 EGT+V PE + +SHG + ++ + +V I+ I Sbjct: 235 EGTMVVERLPEWLEEPVVTGATISHGQIKVSISFLP--KDVKYITA-------------I 279 Query: 297 FKSMADNGISVDFINVTPTEAL----YTVNDDKSEQAIAALQEL-----GLRPSSLSGCA 347 F+ + ++VD I++ P+ +T+ +D E AL+E G + + G A Sbjct: 280 FEEVGKRALNVDMISLVPSNGKVFLSFTILEDHKEDLDEALKEALRDVEGWKSTYEGGFA 339 Query: 348 KXXXXXXXXXXXXXXMARIVEALSSQNIQILQSADSNTTIWVLVKKEDMVQSLRALHAKF 407 K AR EAL + S I LV +E ++L+++ +F Sbjct: 340 KLSIVGVGMRTSPGVAARFFEALERAGVTPELVTTSEIKISCLVPEEKAEEALKSVIEEF 399 Query: 408 EL 409 EL Sbjct: 400 EL 401 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.318 0.133 0.374 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 27,884,910 Number of extensions: 1097283 Number of successful extensions: 2477 Number of sequences better than 1.0e-05: 52 Number of HSP's gapped: 2423 Number of HSP's successfully gapped: 52 Length of query: 411 Length of database: 38,378,431 Length adjustment: 109 Effective length of query: 302 Effective length of database: 25,807,025 Effective search space: 7793721550 Effective search space used: 7793721550 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 117 (49.7 bits)