BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0280.0028.p (302 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value BH1742 [EM] COG0329 Dihydrodipicolinate synthase/N-acetylneurami... 303 3e-82 BS_dapA [EM] COG0329 Dihydrodipicolinate synthase/N-acetylneuram... 301 6e-82 BH2399 [EM] COG0329 Dihydrodipicolinate synthase/N-acetylneurami... 286 3e-77 ML0093 [R] COG1216 Predicted glycosyltransferases 239 4e-63 SP1014 [EM] COG0329 Dihydrodipicolinate synthase/N-acetylneurami... 231 1e-60 >BH1742 [EM] COG0329 Dihydrodipicolinate synthase/N-acetylneuraminate lyase Length = 295 Score = 303 bits (775), Expect = 3e-82, Method: Compositional matrix adjust. Identities = 148/283 (52%), Positives = 201/283 (71%), Gaps = 6/283 (2%) Query: 12 VDFGRLITAMVTPFDRSGEINWEATSRLIDYLIEQKKSEALVVCGTTGESPTLSDEEKLE 71 ++ GR++TAMVTPF+ ++N EAT +L++ LI S+ALVV GTTGESPTL+ +EK+ Sbjct: 1 MNIGRIVTAMVTPFNEQDQVNLEATCQLVNELI-ANGSDALVVGGTTGESPTLTTDEKMA 59 Query: 72 LFAYVLKKAAGRCKIIAGTGTNSTRHSIHLTKEAEKIGVDGVLLVVPYYNKPNQEGLYRH 131 LF V++ A GR +IAGTG+N+TR SI L+K+ E +GVDG++LV PYYNKP+QEGL++H Sbjct: 60 LFHTVVEAAGGRVPVIAGTGSNNTRASIELSKKVEDLGVDGIMLVTPYYNKPSQEGLFQH 119 Query: 132 FAAIAGETKLPVILYNVPSRTSVSMSAATTLRLAGIPNIVATKECQ-SVDQITQIVSACP 190 F AI+ T LP++LYN+P R+ V++ A TTLRLA + NIV+ KE ++DQI +I+ P Sbjct: 120 FKAISESTSLPIMLYNIPGRSGVNLDAETTLRLAELRNIVSIKEASGNLDQIAEIIEHSP 179 Query: 191 ADFRVYXXXXXXXXXXXXXXXYGIISVASHVVGEQMAEMIQAYFSGNVQRAGELHRKLFP 250 ADF VY G++SVASH++G +M EM+Q + SG V+RA +HRKL P Sbjct: 180 ADFSVYSGDDSLTLPLLSIGGTGVVSVASHIIGNEMQEMVQLFLSGQVERAAAIHRKLLP 239 Query: 251 VFKGLFECPQPLPNPSAVKYALGLRGVDVGTVRLPLVPPTEEE 293 + K LF PNP+AVK AL L+G+ VG VRLPLVP T EE Sbjct: 240 LMKTLFMT----PNPTAVKAALELKGLPVGHVRLPLVPLTAEE 278 >BS_dapA [EM] COG0329 Dihydrodipicolinate synthase/N-acetylneuraminate lyase Length = 290 Score = 301 bits (772), Expect = 6e-82, Method: Compositional matrix adjust. Identities = 149/284 (52%), Positives = 198/284 (69%), Gaps = 7/284 (2%) Query: 12 VDFGRLITAMVTPFDRSGEINWEATSRLIDYLIEQKKSEALVVCGTTGESPTLSDEEKLE 71 ++FG + TAM+TPFD G ++++ S LIDYL+ + +++LVV GTTGESPTLS EEK+ Sbjct: 1 MNFGNVSTAMITPFDNKGNVDFQKLSTLIDYLL-KNGTDSLVVAGTTGESPTLSTEEKIA 59 Query: 72 LFAYVLKKAAGRCKIIAGTGTNSTRHSIHLTKEAEKIGVDGVLLVVPYYNKPNQEGLYRH 131 LF Y +K+ GR +IAGTG+N+T+ SI LTK+AE+ GVD V+LV PYYNKP+QEG+Y+H Sbjct: 60 LFEYTVKEVNGRVPVIAGTGSNNTKDSIKLTKKAEEAGVDAVMLVTPYYNKPSQEGMYQH 119 Query: 132 FAAIAGETKLPVILYNVPSRTSVSMSAATTLRLAG-IPNIVATKECQ-SVDQITQIVSAC 189 F AIA ET LPV+LYNVP RT S++ TT+RLA IPN+VA KE ++ IT+I++ Sbjct: 120 FKAIAAETSLPVMLYNVPGRTVASLAPETTIRLAADIPNVVAIKEASGDLEAITKIIAET 179 Query: 190 PADFRVYXXXXXXXXXXXXXXXYGIISVASHVVGEQMAEMIQAYFSGNVQRAGELHRKLF 249 P DF VY G++SVASH+ G M +MI+ Y +G A +H+KL Sbjct: 180 PEDFYVYSGDDALTLPILSVGGRGVVSVASHIAGTDMQQMIKNYTNGQTANAALIHQKLL 239 Query: 250 PVFKGLFECPQPLPNPSAVKYALGLRGVDVGTVRLPLVPPTEEE 293 P+ K LF+ P P P VK AL LRG+DVG+VRLPLVP TE+E Sbjct: 240 PIMKELFKAPNPAP----VKTALQLRGLDVGSVRLPLVPLTEDE 279 >BH2399 [EM] COG0329 Dihydrodipicolinate synthase/N-acetylneuraminate lyase Length = 292 Score = 286 bits (732), Expect = 3e-77, Method: Compositional matrix adjust. Identities = 140/292 (47%), Positives = 192/292 (65%), Gaps = 6/292 (2%) Query: 12 VDFGRLITAMVTPFDRSGEINWEATSRLIDYLIEQKKSEALVVCGTTGESPTLSDEEKLE 71 +DFG ++TAMVTPFD G ++ E RL+++L+ +E LVV GTTGESPTL +EEKL Sbjct: 1 MDFGHVLTAMVTPFDAKGNVDLEKVERLVEFLLANG-TEGLVVAGTTGESPTLREEEKLA 59 Query: 72 LFAYVLKKAAGRCKIIAGTGTNSTRHSIHLTKEAEKIGVDGVLLVVPYYNKPNQEGLYRH 131 LF V+ R +IAGTG+N+T S LTK+A +GVDG++ V PYYNKP+QEG+Y H Sbjct: 60 LFEKVVTVVNKRVPVIAGTGSNNTYVSAQLTKKATALGVDGIMAVTPYYNKPSQEGMYAH 119 Query: 132 FAAIAGETKLPVILYNVPSRTSVSMSAATTLRLAGIPNIVATKECQ-SVDQITQIVSACP 190 FAAIA T LPV+LYN+PSR+ V++SA T ++L+ IPNI + KE +D + ++ P Sbjct: 120 FAAIAEATHLPVMLYNIPSRSVVNLSAETIVKLSYIPNITSLKEANGDLDHMVSVIEQTP 179 Query: 191 ADFRVYXXXXXXXXXXXXXXXYGIISVASHVVGEQMAEMIQAYFSGNVQRAGELHRKLFP 250 F +Y G++SVASH++G +M +M+ A+ G+V+ A LHRKL P Sbjct: 180 DTFHLYSGDDSLTLPALSIGAKGVVSVASHIIGPEMQQMMMAFRGGDVEHAANLHRKLLP 239 Query: 251 VFKGLFECPQPLPNPSAVKYALGLRGVDVGTVRLPLVPPTEEEASFIAALLE 302 + KGLF PNP+ VK AL ++G D G VRLPLVPPT E+ + L+E Sbjct: 240 IMKGLFTA----PNPTCVKAALQIKGFDTGGVRLPLVPPTSEQRQHLQFLIE 287 >ML0093 [R] COG1216 Predicted glycosyltransferases Length = 297 Score = 239 bits (610), Expect = 4e-63, Method: Compositional matrix adjust. Identities = 122/288 (42%), Positives = 172/288 (59%), Gaps = 6/288 (2%) Query: 11 KVDFGRLITAMVTPFDRSGEINWEATSRLIDYLIEQKKSEALVVCGTTGESPTLSDEEKL 70 K+ R+ITA+VTPF +G+IN+ A +LI+ L+ +E L++ GTT ESPTL+ +E+L Sbjct: 9 KLQNARIITALVTPFKENGQINFGAFPKLIEDLL-ANHTEGLILAGTTAESPTLTHDEEL 67 Query: 71 ELFAYVLKKAAGRCKIIAGTGTNSTRHSIHLTKEAEKIG-VDGVLLVVPYYNKPNQEGLY 129 +FA V K GR +IAG GTN TR S+ KE ++G +D L V PYYNKP+QEG+Y Sbjct: 68 AIFAAVNKIVDGRIPLIAGVGTNDTRDSVEFVKEVAELGYIDAGLAVTPYYNKPSQEGIY 127 Query: 130 RHFAAIAGETKLPVILYNVPSRTSVSMSAATTLRLAGIPNIVATKECQSVDQITQIVSAC 189 +HF AIA + LP+ILYN+P R + T LRLA + N++A KEC + D + ++ Sbjct: 128 QHFKAIATASDLPIILYNIPGRVVTEIQVETILRLAELENVIAIKECTNTDNLAYLIEKL 187 Query: 190 PADFRVYXXXXXXXXXXXXXXXYGIISVASHVVGEQMAEMIQAYFSGNVQRAGELHRKLF 249 P DF VY G+ISVASH++G++ EM G++Q+A + RK+ Sbjct: 188 PKDFLVYTGEDGLAFHTKALGGQGVISVASHILGQEFFEMFAEIDQGSIQKAAAIQRKIL 247 Query: 250 PVFKGLFECPQPLPNPSAVKYALGLRGVDVGTVRLPLVPPTEEEASFI 297 P LF P P +K L +G +VG +RLPLV T EE+ I Sbjct: 248 PKINALFSVTSPAP----IKTVLNAKGYEVGGLRLPLVACTTEESKII 291 >SP1014 [EM] COG0329 Dihydrodipicolinate synthase/N-acetylneuraminate lyase Length = 311 Score = 231 bits (588), Expect = 1e-60, Method: Compositional matrix adjust. Identities = 121/283 (42%), Positives = 165/283 (58%), Gaps = 6/283 (2%) Query: 16 RLITAMVTPFDRSGEINWEATSRLIDYLIEQKKSEALVVCGTTGESPTLSDEEKLELFAY 75 ++ITA +TPF G IN++A LI++L+ ++ +++ GTT ESPTL+ +E+LELFA Sbjct: 10 KIITAFITPFHEDGSINFDAIPALIEHLLAHH-TDGILLAGTTAESPTLTHDEELELFAA 68 Query: 76 VLKKAAGRCKIIAGTGTNSTRHSIHLTKE-AEKIGVDGVLLVVPYYNKPNQEGLYRHFAA 134 V K GR +IAG GTN TR SI KE AE G L +VPYYNKP+QEG+Y+HF Sbjct: 69 VQKVVNGRVPLIAGVGTNDTRDSIEFVKEVAEFGGFAAGLAIVPYYNKPSQEGMYQHFKT 128 Query: 135 IAGETKLPVILYNVPSRTSVSMSAATTLRLAGIPNIVATKECQSVDQITQIVSACPADFR 194 IA + LP+I+YN+P R V ++ T LRLA PNI+ KEC S+ + ++ P +F Sbjct: 129 IADASDLPIIIYNIPGRVVVELTPETMLRLADHPNIIGVKECTSLANMAYLIEHKPEEFL 188 Query: 195 VYXXXXXXXXXXXXXXXYGIISVASHVVGEQMAEMIQAYFSGNVQRAGELHRKLFPVFKG 254 +Y G+ISVASH G++M EM A ++++A + RK P Sbjct: 189 IYTGEDGDAFHAMNLGADGVISVASHTNGDEMHEMFTAIAESDMKKAAAIQRKFIPKVNA 248 Query: 255 LFECPQPLPNPSAVKYALGLRGVDVGTVRLPLVPPTEEEASFI 297 LF P P P VK L G + G RLPLVP EE+A I Sbjct: 249 LFSYPSPAP----VKAILNYMGFEAGPTRLPLVPAPEEDAKRI 287 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.318 0.135 0.387 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 20,444,266 Number of extensions: 815083 Number of successful extensions: 3466 Number of sequences better than 1.0e-05: 72 Number of HSP's gapped: 3357 Number of HSP's successfully gapped: 72 Length of query: 302 Length of database: 38,378,431 Length adjustment: 106 Effective length of query: 196 Effective length of database: 26,153,027 Effective search space: 5125993292 Effective search space used: 5125993292 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 115 (48.9 bits)