BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0280.0020.p (412 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value BH2393 [R] COG0612 Predicted Zn-dependent peptidases 304 2e-82 lin1429 [R] COG0612 Predicted Zn-dependent peptidases 266 6e-71 SA1121 [R] COG0612 Predicted Zn-dependent peptidases 226 6e-59 BS_ymfG [R] COG0612 Predicted Zn-dependent peptidases 188 2e-47 L32666 [R] COG0612 Predicted Zn-dependent peptidases 170 4e-42 >BH2393 [R] COG0612 Predicted Zn-dependent peptidases Length = 431 Score = 304 bits (778), Expect = 2e-82, Method: Compositional matrix adjust. Identities = 158/415 (38%), Positives = 242/415 (58%), Gaps = 5/415 (1%) Query: 1 MRIHVLPTKAFKTFAISLYAGVPLDEHTVTPTALIPFVLRRGTANYPETTQFRERLEELY 60 + +H+L T +KT + L PL++ TV AL+ VL+ TA P + R RL++LY Sbjct: 14 LHVHLLKTDKYKTNTLMLMVKAPLEKETVAKRALLANVLQNSTAKSPTRSDLRNRLDDLY 73 Query: 61 GAGFGFDIYKRGDYQIVQFRMDTINDSFVQSKESLLGESFAFLGEVLTKPLLEDGSFRAS 120 GA D+ K+G+ I+ R+D N+ F+ L + + L EVL +P + F + Sbjct: 74 GATLATDVQKKGEQHIITIRLDVANEEFLSDSTPLFEKGLSLLAEVLLEPKVSGNKFDEA 133 Query: 121 YVATERETVRKKLEAIVNDKIRYAAERCIEEMCRQEPYRLHPLGQRADLDGITPESLYQS 180 V +E+ ++ +++E++ +DK+RY++ R EEMC+ EP+ L G R ++ ++ E LY+ Sbjct: 134 VVKSEKRSLLERIESLYDDKMRYSSLRVTEEMCKDEPFGLSVYGTREQVEQLSAEELYRY 193 Query: 181 YHSWLGGAILDLYVVGDTTPEEVEKLVTRHFGRTHGNAAGYSSNFSPVA-----VTEVRT 235 Y L +DL+V+G+ + V F + ++ P + V E R Sbjct: 194 YEQMLHSDRIDLFVLGEKGDPSLMSTVEDTFSKLATYQKEAKTDSVPASIPSPEVKEARE 253 Query: 236 VEEKLDVNQGKLNMGLRTPITYKDDRYASALMYNGILGGYPHSKLFVNVREKESLAYYAS 295 V EK DV QGKLN+G R TY D Y + + NG+ GG+ HSKLF+NVREKESLAYYA+ Sbjct: 254 VIEKQDVKQGKLNIGFRAYTTYGDPDYVAMQVMNGLFGGFSHSKLFINVREKESLAYYAA 313 Query: 296 SRYDGHKGIGTIQSGIETQNYSKAVDIIRKQLDELQAGNISDLELSQTKAMIRNLLSEIQ 355 SR++ HKG+ + SGIE NY KAV II++QL+E++ G +D EL QT+AM++N L E Sbjct: 314 SRFESHKGLLIVMSGIEFTNYEKAVTIIKEQLEEMKNGRFTDKELEQTRAMLKNQLLETA 373 Query: 356 DSAFEMISFDFNRQLSGKDRSAQELLAQVDSTGAEDVKAAAGTFELDTIYFLTGK 410 D I ++ +S +R+ ++ LA++D+ E V AAA LDTIYFL G+ Sbjct: 374 DVPRGQIEVMYHNIVSNHERTLEKWLAEIDAITKEQVVAAANKVVLDTIYFLKGE 428 >lin1429 [R] COG0612 Predicted Zn-dependent peptidases Length = 430 Score = 266 bits (679), Expect = 6e-71, Method: Compositional matrix adjust. Identities = 141/410 (34%), Positives = 233/410 (56%), Gaps = 2/410 (0%) Query: 3 IHVLPTKAFKTFAISLYAGVPLDEHTVTPTALIPFVLRRGTANYPETTQFRERLEELYGA 62 + ++PT+ +K+ I PL+ TVT +L+ +L + YP T FR++L LYGA Sbjct: 15 LTIVPTQKYKSNKIVFKFRAPLERETVTKRSLLSILLETNSEKYPTQTAFRKQLANLYGA 74 Query: 63 GFGFDIYKRGDYQIVQFRMDTINDSFVQSKESLLGESFAFLGEVLTKPLLEDGSFRASYV 122 F K+G+ ++ D I+ +V E++L ++FAF+ + + +P +G+F V Sbjct: 75 NFYTTTAKKGNEHVLTVVFDMIDGQYVSDGENILKDAFAFIEQAIFQPNATNGAFDEEIV 134 Query: 123 ATERETVRKKLEAIVNDKIRYAAERCIEEMCRQEPYRLHPLGQRADLDGITPESLYQSYH 182 E+E ++ LE I +DKIR+A++R +EEM R + +R G D+D IT + LY+ Y Sbjct: 135 VREKENLKSSLEGIYDDKIRFASKRLVEEMFRDDEFRFGAAGVLEDVDAITAKDLYEYYL 194 Query: 183 SWLGGAILDLYVVGDTTPEEVEKLVTRHFGRTHGNAAGYSSNFSPVAVTEVRTVEEKLDV 242 ++ ++++++ GD T EEV L+ + G ++ A E R + E+ + Sbjct: 195 KFIKEDVIEIFICGDVTKEEVMPLIEKMAFSPRPERKGVF--YTKEAPKETRVIHEQQAI 252 Query: 243 NQGKLNMGLRTPITYKDDRYASALMYNGILGGYPHSKLFVNVREKESLAYYASSRYDGHK 302 NQGKL +G +T + DD + + + NG+LGG+ +SK+F+NVREK SLAYYASSR D K Sbjct: 253 NQGKLVLGYQTETLFGDDDFVALQLANGLLGGFANSKIFINVREKASLAYYASSRIDSFK 312 Query: 303 GIGTIQSGIETQNYSKAVDIIRKQLDELQAGNISDLELSQTKAMIRNLLSEIQDSAFEMI 362 G I +GI+ NY +A+ II +Q+ ++ G+ ++ EL+QTK M+ N L E D A +I Sbjct: 313 GYMIISAGIDEVNYKQALTIIEEQIVAMKQGDFTEDELNQTKEMLINQLLETNDQAQGLI 372 Query: 363 SFDFNRQLSGKDRSAQELLAQVDSTGAEDVKAAAGTFELDTIYFLTGKGE 412 +N L + + + ++ ++V AA + DTIYFL+ GE Sbjct: 373 ELAYNNVLREANLDLENWIIKIKQATKQEVVAAINKIKPDTIYFLSKGGE 422 >SA1121 [R] COG0612 Predicted Zn-dependent peptidases Length = 421 Score = 226 bits (575), Expect = 6e-59, Method: Compositional matrix adjust. Identities = 131/412 (31%), Positives = 214/412 (51%) Query: 1 MRIHVLPTKAFKTFAISLYAGVPLDEHTVTPTALIPFVLRRGTANYPETTQFRERLEELY 60 + I V PT FKT I PL+ T+T +L+ +L R T +P F L +LY Sbjct: 9 IHIKVSPTTKFKTTTIVFKFMAPLEYDTITARSLLSKLLVRATKKWPTDKSFNNHLADLY 68 Query: 61 GAGFGFDIYKRGDYQIVQFRMDTINDSFVQSKESLLGESFAFLGEVLTKPLLEDGSFRAS 120 GA I K D ++ F ++ +N+ ++++ ESL + L E++ PL+E+ +F + Sbjct: 69 GAYVNSTISKFKDQHVITFSLEIVNERYLRNGESLFNQGLDLLQEIIWNPLIENKAFNDN 128 Query: 121 YVATERETVRKKLEAIVNDKIRYAAERCIEEMCRQEPYRLHPLGQRADLDGITPESLYQS 180 +V E+ + KK+EA+V++K +Y+ + ++ M E Y+ GQ + IT E+LY + Sbjct: 129 FVNQEKTLLAKKIEAMVDNKAQYSFLKLLDHMFENEAYKYLSTGQLEQIPHITAETLYHT 188 Query: 181 YHSWLGGAILDLYVVGDTTPEEVEKLVTRHFGRTHGNAAGYSSNFSPVAVTEVRTVEEKL 240 Y S + +YVVG+ PE VEK + F + + + + EV + E Sbjct: 189 YQSMINNDQCSVYVVGNVEPESVEKQIREKFALKPFDKHQFQHSTHHLHDEEVDYIVEYD 248 Query: 241 DVNQGKLNMGLRTPITYKDDRYASALMYNGILGGYPHSKLFVNVREKESLAYYASSRYDG 300 DV+Q KLNMG R P Y YA+ +++N + GG P S LF VREK+SLAY S+ DG Sbjct: 249 DVDQAKLNMGYRFPTQYGQSGYAAFVVFNMMFGGDPSSVLFNEVREKQSLAYSIHSQIDG 308 Query: 301 HKGIGTIQSGIETQNYSKAVDIIRKQLDELQAGNISDLELSQTKAMIRNLLSEIQDSAFE 360 G + SG+ + Y A D I + ++++AG+ ++ +L K +I + E +D Sbjct: 309 KNGYLFVLSGVSSDKYETAKDTIISEFEKIKAGDFTEEKLELAKKVIISHRYESEDRPKS 368 Query: 361 MISFDFNRQLSGKDRSAQELLAQVDSTGAEDVKAAAGTFELDTIYFLTGKGE 412 +I N+ L + +S + + + ED+ + A LDTIY LT G+ Sbjct: 369 IIEIMHNQILLEQPQSKETFINDIQKVSREDIVSVAEKAFLDTIYVLTKGGD 420 >BS_ymfG [R] COG0612 Predicted Zn-dependent peptidases Length = 81 Score = 188 bits (477), Expect = 2e-47, Method: Compositional matrix adjust. Identities = 0/234 (0%), Positives = 0/234 (0%), Gaps = 1/234 (0%) Query: 152 MCRQEPYRLHPLGQRADLDGITPESLYQSYHSWLGGAILDLYVVGDTTPEEVEKLVTRHF 211 M + + + + + + + Sbjct: 1 MLQMLTLEEWAAEKYRSNPPSVSTLRRYAKQNLFCPPAMKQGRLWRVREDAELVGELVTP 60 Query: 212 GRTHGNAAGYSSNFSPVAVTEVRTVEEKLDVNQGKLNMGLRTPITYKDDRYASALMYNGI 271 Sbjct: 61 - 119 Query: 272 LGGYPHSKLFVNVREKESLAYYASSRYDGHKGIGTIQSGIETQNYSKAVDIIRKQLDELQ 331 Sbjct: 120 179 Query: 332 AGNISDLELSQTKAMIRNLLSEIQDSAFEMISFDFNRQLSGKDRSAQELLAQVD 385 Sbjct: 180 233 >L32666 [R] COG0612 Predicted Zn-dependent peptidases Length = 418 Score = 170 bits (430), Expect = 4e-42, Method: Compositional matrix adjust. Identities = 116/409 (28%), Positives = 191/409 (46%), Gaps = 8/409 (1%) Query: 3 IHVLPTKAFKTFAISLYAGVPLDEHTVTPTALIPFVLRRGTANYPETTQFRERLEELYGA 62 +H++ FKT + + + T+ LI +L A Y F +L E+YGA Sbjct: 2 LHLIKESKFKTVRLMVRFREEMSADTLGKRVLISNMLESTNAVYQTGKDFSRKLSEMYGA 61 Query: 63 GFGFDIYKRGDYQIVQFRMDTINDSFVQSKESLLGESFAFLGEVLTKPLLEDGSFRASYV 122 F + K+G ++ M +N V LG + FL + +P +G F Sbjct: 62 SFTTGVAKKGKQHLLSLNMSIVNPKLVDF--DTLGAAVDFLKIAIFQPDAANGQFNPEIF 119 Query: 123 ATERETVRKKLEAIVNDKIRYAAERCIEEMCRQEPYRLHPLGQRADLDGITPESLYQSYH 182 E+ + L ++ +D+ YA+ + E L +G + P+++++ Y Sbjct: 120 KREQRNLLHYLASMNDDRSYYASRQLANLFFEDENQALPSVGTSELIAKENPKAVFEYYQ 179 Query: 183 SWLGGAILDLYVVGDTTPEEVEKLVTRHFGRTHGNAAGYSSNFS-PVAVTEVRTVEEKLD 241 L +D++V+GD EK + F + S S ++TE V ++ + Sbjct: 180 KMLTDNAIDIFVLGDVD----EKRMIERFSDFNFTDRAVSKEISYQQSLTESSVVTDEKE 235 Query: 242 VNQGKLNMGLRTPITYKDDRYASALMYNGILGGYPHSKLFVNVREKESLAYYASSRYDGH 301 V Q L + PI Y D Y + + NG+LGG+ HSKLF NVREK SLAY SS +D Sbjct: 236 VAQSILQFAYQMPIAYGDKNYLALQVMNGLLGGFAHSKLFTNVREKASLAYSISSTFDSF 295 Query: 302 KGIGTIQSGIETQNYSKAVDIIRKQLDELQAGNISDLELSQTKAMIRNLLSEIQDSAFEM 361 G I +GI+ +NY +A +I +QL+ +++G+ ++LE+ QTK M+RN QDS Sbjct: 296 TGFLKIAAGIDVENYEEAKSLIFEQLEAIKSGDFTELEVEQTKTMLRNAYFVGQDSPSNT 355 Query: 362 ISFDFNRQL-SGKDRSAQELLAQVDSTGAEDVKAAAGTFELDTIYFLTG 409 I ++ + L K E L ++S D+ + A + L YF+ G Sbjct: 356 IELEYVKALIPDKFLPMSEFLNALESVSKADLISVAKSLNLQAEYFMKG 404 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.317 0.135 0.382 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 30,371,241 Number of extensions: 1221409 Number of successful extensions: 4379 Number of sequences better than 1.0e-05: 20 Number of HSP's gapped: 4376 Number of HSP's successfully gapped: 20 Length of query: 412 Length of database: 38,378,431 Length adjustment: 109 Effective length of query: 303 Effective length of database: 25,807,025 Effective search space: 7819528575 Effective search space used: 7819528575 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 117 (49.7 bits)