BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0280.0018.p (255 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value CAC2626 [IQR] COG1028 Dehydrogenases with different specificitie... 176 3e-44 lin1431 [IQR] COG1028 Dehydrogenases with different specificitie... 166 3e-41 STM1724_1 [EH] COG0512 Anthranilate/para-aminobenzoate synthases... 165 7e-41 STM1724_2 [E] COG0547 Anthranilate phosphoribosyltransferase 165 7e-41 TM1724 [IQR] COG1028 Dehydrogenases with different specificities... 165 7e-41 >CAC2626 [IQR] COG1028 Dehydrogenases with different specificities (related to short-chain alcohol dehydrogenases) Length = 248 Score = 176 bits (447), Expect = 3e-44, Method: Compositional matrix adjust. Identities = 96/238 (40%), Positives = 140/238 (58%) Query: 17 LITXXXXXXXXXXXERFASVGMNIVIHYMNSHEAANDVARRCMALGAKVMTVAADMKDRS 76 LIT E A +G+ +VI+Y N + A D + G D+ S Sbjct: 11 LITGSSRGIGKSIAEEMAKMGIEVVINYRNDEKGALDTLKSIKNSGGIAEVYKCDITSYS 70 Query: 77 QLVRMAERLAASGMQPDILVNNAGKAHYGMLADVTEEEWDEIMAVNLKGTFLCSQIFMPY 136 ++ M E + + + D+LVNNAG + G+ D+ E +++EIM + +G F C+ + + Y Sbjct: 71 EVKNMFEFIISKFGKIDLLVNNAGISKIGLFIDMKENDYNEIMDNDFRGVFNCTSLAVKY 130 Query: 137 MVSQRYGRIINVSSVWGISGASCEVAYSASKGGVNAFTKALAKELAPSGVTVNAVAPGAV 196 M+ ++ G IIN+SS+WG GASCEV YSA+KG VN+FTKAL KELAPSG+ VNA++PG + Sbjct: 131 MLERKSGSIINISSIWGNVGASCEVLYSAAKGAVNSFTKALGKELAPSGIRVNAISPGVI 190 Query: 197 QTAMLANLQADEMRMLEDEIPAGRLASPEEISSLVYFLALPESGYITGQIISPNGGWI 254 T M + DE LE+EIP R EI+ + FLA E+ YIT Q+I+ +GG + Sbjct: 191 DTEMNGSFSNDEKNDLENEIPMCRFGKTSEIAKIAIFLASEEASYITAQVITADGGML 248 >lin1431 [IQR] COG1028 Dehydrogenases with different specificities (related to short-chain alcohol dehydrogenases) Length = 200 Score = 166 bits (420), Expect = 3e-41, Method: Compositional matrix adjust. Identities = 0/222 (0%), Positives = 0/222 (0%), Gaps = 4/222 (1%) Query: 33 FASVGMNIVIHYMNSHEAANDVARRCMALGAKVMTVAADMKDRSQLVRMAERLAASGMQP 92 + + + + A +L + Sbjct: 25 NIRQQQRLEQAAYEMTALRAATIELGPLVRGLPHLRTIHRQLYQDIFDWAGQL----REV 80 Query: 93 DILVNNAGKAHYGMLADVTEEEWDEIMAVNLKGTFLCSQIFMPYMVSQRYGRIINVSSVW 152 DI + H+ + ++ ++ +++ V Sbjct: 81 DIYQGDTPFCHFAYIEKEGNALMQDLEEEGYLVGLEKAKFVERLAHYYCEINVLHPFRVG 140 Query: 153 GISGASCEVAYSASKGGVNAFTKALAKELAPSGVTVNAVAPGAVQTAMLANLQADEMRML 212 A G + + KE A+ + + + ++ Sbjct: 141 SGLAQRIFFEQLAIHAGYQLSWQGIEKEAWNQANQSGAMGDLTALQMIFSKVVSEAGESE 200 Query: 213 EDEIPAGRLASPEEISSLVYFLALPESGYITGQIISPNGGWI 254 Sbjct: 201 242 >STM1724_1 [EH] COG0512 Anthranilate/para-aminobenzoate synthases component II Length = 197 Score = 165 bits (417), Expect = 7e-41, Method: Compositional matrix adjust. Identities = 0/222 (0%), Positives = 0/222 (0%), Gaps = 1/222 (0%) Query: 33 FASVGMNIVIHYMNSHEAANDVARRCMALGAKVMTVAADMKDRSQLVRMAERLAASGMQP 92 +V + + L + R + Sbjct: 25 GHNVVIYRNHIPA-QTLIDRLATMKNPVLMLSPGPGVPSEAGCMPELLTRLRGKLPIIGI 83 Query: 93 DILVNNAGKAHYGMLADVTEEEWDEIMAVNLKGTFLCSQIFMPYMVSQRYGRIINVSSVW 152 + +A+ G + E + ++ G + + + P V++ + + + Sbjct: 84 CLGHQAIVEAYGGYVGQAGEILHGKASSIEHDGQAMFAGLANPLPVARYHSLVGSNVPAG 143 Query: 153 GISGASCEVAYSASKGGVNAFTKALAKELAPSGVTVNAVAPGAVQTAMLANLQADEMRML 212 Sbjct: 144 203 Query: 213 EDEIPAGRLASPEEISSLVYFLALPESGYITGQIISPNGGWI 254 Sbjct: 204 245 >STM1724_2 [E] COG0547 Anthranilate phosphoribosyltransferase Length = 334 Score = 165 bits (417), Expect = 7e-41, Method: Compositional matrix adjust. Identities = 17/222 (7%), Positives = 40/222 (18%), Gaps = 1/222 (0%) Query: 33 FASVGMNIVIHYMNSHEAANDVARRCMALGAKVMTVAADMKDRSQLVRMAERLAASGMQP 92 F++V + A + R A +++ + R A Sbjct: 25 FSAVVRGELKPEQ-LAAALVSMKIRGEHPNEIAGAATALLENAAPFPRPEYLFADIVGTG 83 Query: 93 DILVNNAGKAHYGMLADVTEEEWDEIMAVNLKGTFLCSQIFMPYMVSQRYGRIINVSSVW 152 N+ + + S + Sbjct: 84 GDGSNSINISTASAFVAAACGLKVAKHGNRSVSSKSGSSDLLAAFGINLDMNADKSRQAL 143 Query: 153 GISGASCEVAYSASKGGVNAFTKALAKELAPSGVTVNAVAPGAVQTAMLANLQADEMRML 212 G A G +A + + + A L + + E+ + Sbjct: 144 DELGVCFLFAPKYHTGLRHAMPVRQQLKTRTLFNVLGPLINPAHPPLALIGVYSPELVLP 203 Query: 213 EDEIPAGRLASPEEISSLVYFLALPESGYITGQIISPNGGWI 254 E + + + Sbjct: 204 IAETLRVLGYQRAAVVHSGGMDEVSLHAPTIVAELHDGEIKS 245 >TM1724 [IQR] COG1028 Dehydrogenases with different specificities (related to short-chain alcohol dehydrogenases) Length = 246 Score = 165 bits (417), Expect = 7e-41, Method: Compositional matrix adjust. Identities = 88/222 (39%), Positives = 125/222 (56%), Gaps = 1/222 (0%) Query: 33 FASVGMNIVIHYMNSHEAANDVARRCMALGAKVMTVAADMKDRSQLVRMAERLAASGMQP 92 FA G ++ + S E + + + L KV ++ DR Q+ + E++ + Sbjct: 25 FAQEGATVIAGDI-SKENLDSLVKEAEGLPGKVDPYVLNVTDRDQIKEVVEKVVQKYGRI 83 Query: 93 DILVNNAGKAHYGMLADVTEEEWDEIMAVNLKGTFLCSQIFMPYMVSQRYGRIINVSSVW 152 D+LVNNAG +L + EE+WD ++ VNLKG F +Q+ +PYM+ QR G I+NVSSV Sbjct: 84 DVLVNNAGITRDALLVRMKEEDWDAVINVNLKGVFNVTQMVVPYMIKQRNGSIVNVSSVV 143 Query: 153 GISGASCEVAYSASKGGVNAFTKALAKELAPSGVTVNAVAPGAVQTAMLANLQADEMRML 212 GI G + Y+ASK GV TK AKELA + VNAVAPG ++T M L Sbjct: 144 GIYGNPGQTNYAASKAGVIGMTKTWAKELAGRNIRVNAVAPGFIETPMTEKLPEKARETA 203 Query: 213 EDEIPAGRLASPEEISSLVYFLALPESGYITGQIISPNGGWI 254 IP GR PEE++ ++ FLA ES Y+TGQ+I +GG + Sbjct: 204 LSRIPLGRFGKPEEVAQVILFLASDESSYVTGQVIGIDGGLV 245 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.317 0.130 0.370 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 16,541,137 Number of extensions: 581215 Number of successful extensions: 2336 Number of sequences better than 1.0e-05: 329 Number of HSP's gapped: 1977 Number of HSP's successfully gapped: 329 Length of query: 255 Length of database: 38,378,431 Length adjustment: 104 Effective length of query: 151 Effective length of database: 26,383,695 Effective search space: 3983937945 Effective search space used: 3983937945 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 114 (48.5 bits)