BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0280.0010.p (429 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value STM2293 [R] COG1058 Predicted nucleotide-utilizing enzyme relate... 91 4e-18 PA3618 [R] COG1546 Uncharacterized protein (competence- and mito... 90 6e-18 STM2830 [R] COG1546 Uncharacterized protein (competence- and mit... 84 5e-16 ZygaD [R] COG1546 Uncharacterized protein (competence- and mitom... 82 2e-15 ECs3557 [R] COG1546 Uncharacterized protein (competence- and mit... 82 2e-15 >STM2293 [R] COG1058 Predicted nucleotide-utilizing enzyme related to molybdopterin-biosynthesis enzyme MoeA Length = 398 Score = 90.5 bits (223), Expect = 4e-18, Method: Compositional matrix adjust. Identities = 82/289 (28%), Positives = 131/289 (45%), Gaps = 12/289 (4%) Query: 1 MKAEIIAVGTELLLGQIVNSNAQFLSLELAALGIDVFFQTVVGDNSSRLQQAIEIARSRA 60 + E+++ G E+L GQIV++NA +L+ G+ + + VGDN L + A Sbjct: 2 LNVEMLSTGDEVLHGQIVDTNAAWLADFFFHQGVPLSRRNTVGDNLDSLVAILRERSQHA 61 Query: 61 DVILFTGGIGPTEDDLTKDALAASLGRSLHIDQLAMDHVQRFFDERKIVMTENNRKQALI 120 DV++ GG+GPT DDL+ A A + G L + Q + ++R+F +R VM +NRKQA + Sbjct: 62 DVLIVNGGLGPTSDDLSALAAATAKGEGLVLHQAWLTEMERYFQQRGRVMAPSNRKQAEL 121 Query: 121 IDGATPLPNETXXXXXXXXXXXXKYYIVLPGPPREMKPMFTGQAKPWLQQHALTGEMPIY 180 A + N PG P E K M + P L+ E P+ Sbjct: 122 PASAEFINNPLGTACGFALQLNRCLMFFTPGVPSEFKVMVEKEILPRLRARFSLPEPPLC 181 Query: 181 SKMLKFAGIGESLLEDKLIDLIRNQSDPTIAPYAKEGEVTVRISTKAP-SEREAMQKL-- 237 ++ F G ES L +L L P + + + + P ++REAM L Sbjct: 182 LRLTTF-GRSESDLAQRLDSL---PLPPGVTMGYRSSMPIIELKLTGPATQREAMLALWP 237 Query: 238 EVLETTIQEILPEHMYANIDVPLEQLIVDWMADAGLTLSAAESCTGGLL 286 EV Q ++ E ++P + I + + L+L+ +E T GLL Sbjct: 238 EVKRVAGQNLIFE---GTENLPAQ--IARRLQERQLSLTLSEQYTSGLL 281 >PA3618 [R] COG1546 Uncharacterized protein (competence- and mitomycin-induced) Length = 168 Score = 90.1 bits (222), Expect = 6e-18, Method: Compositional matrix adjust. Identities = 57/135 (42%), Positives = 76/135 (56%), Gaps = 5/135 (3%) Query: 272 GLTLSAAESCTGGLLMESITNIPGSASMFLGGIVCYSNEMKKKLLNVPAALLEGPDAPGA 331 G +S AESCTGG + E+IT IPGS+ F G V YSN K L VP +L A GA Sbjct: 23 GEQVSTAESCTGGGIAEAITRIPGSSGWFEAGYVTYSNRQKTLQLEVPESLF---PAVGA 79 Query: 332 VSREVAEVLADQVRMIADSDFGLSVTGVAGP-GYSERKPVGLVFVGLAERGRPTQVYELN 390 VSREV E + + + + F ++V+GVAGP G S KPVG V++ A+ G Sbjct: 80 VSREVVEAMVRGAQRHSGARFAVAVSGVAGPDGGSPEKPVGTVWLAWAD-GERLVSERCQ 138 Query: 391 LKGTRENIRLRTVKS 405 G R+ +R +TV + Sbjct: 139 FNGDRDAVRRQTVAT 153 >STM2830 [R] COG1546 Uncharacterized protein (competence- and mitomycin-induced) Length = 165 Score = 84.0 bits (206), Expect = 5e-16, Method: Compositional matrix adjust. Identities = 51/129 (39%), Positives = 71/129 (55%), Gaps = 4/129 (3%) Query: 272 GLTLSAAESCTGGLLMESITNIPGSASMFLGGIVCYSNEMKKKLLNVPAALLEGPDAPGA 331 G T++ AESCTGG L ++IT+I GS++ F G V YSNE K +++ V L GA Sbjct: 21 GATVTTAESCTGGWLAKAITDIAGSSAWFERGFVTYSNEAKAQMIGVREETLA---QHGA 77 Query: 332 VSREVAEVLADQVRMIADSDFGLSVTGVAGP-GYSERKPVGLVFVGLAERGRPTQVYELN 390 VS V +A A +DF ++++G+AGP G SE KPVG V+ A Sbjct: 78 VSEPVVVEMAIGALKAARADFAVAISGIAGPDGGSEEKPVGTVWFAFASVSGEGITRREC 137 Query: 391 LKGTRENIR 399 G RE++R Sbjct: 138 FSGDRESVR 146 >ZygaD [R] COG1546 Uncharacterized protein (competence- and mitomycin-induced) Length = 165 Score = 81.6 bits (200), Expect = 2e-15, Method: Compositional matrix adjust. Identities = 52/130 (40%), Positives = 72/130 (55%), Gaps = 6/130 (4%) Query: 272 GLTLSAAESCTGGLLMESITNIPGSASMFLGGIVCYSNEMKKKLLNVPAALLEGPDAPGA 331 G T++ AESCTGG + + IT+I GS++ F G V YSNE K +++ V L GA Sbjct: 21 GATVTTAESCTGGWVAKVITDIAGSSAWFERGFVTYSNEAKAQMIGVREETLA---QHGA 77 Query: 332 VSREVAEVLADQVRMIADSDFGLSVTGVAGP-GYSERKPVGLVFVGLA-ERGRPTQVYEL 389 VS V +A A +D+ +S++G+AGP G SE KPVG V+ A RG E Sbjct: 78 VSEPVVVEMAIGALKAARADYAVSISGIAGPDGGSEEKPVGTVWFAFATARGEGITRREC 137 Query: 390 NLKGTRENIR 399 G R+ +R Sbjct: 138 -FSGDRDAVR 146 >ECs3557 [R] COG1546 Uncharacterized protein (competence- and mitomycin-induced) Length = 165 Score = 81.6 bits (200), Expect = 2e-15, Method: Compositional matrix adjust. Identities = 52/130 (40%), Positives = 73/130 (56%), Gaps = 6/130 (4%) Query: 272 GLTLSAAESCTGGLLMESITNIPGSASMFLGGIVCYSNEMKKKLLNVPAALLEGPDAPGA 331 G T++ AESCTGG + + IT+I GS++ F G V YSNE K +++ V L GA Sbjct: 21 GATVTTAESCTGGWVAKVITDIAGSSAWFERGFVTYSNEAKAQMIGVREETLA---QHGA 77 Query: 332 VSREVAEVLADQVRMIADSDFGLSVTGVAGP-GYSERKPVGLVFVGLA-ERGRPTQVYEL 389 VS V +A A +D+ +S++G+AGP G SE KPVG V+ A RG + E Sbjct: 78 VSEPVVVEMAIGALKAARADYAVSISGIAGPDGGSEEKPVGTVWFAFATARGEGITLREC 137 Query: 390 NLKGTRENIR 399 G R+ +R Sbjct: 138 -FSGDRDAVR 146 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.316 0.134 0.375 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 28,969,999 Number of extensions: 1201079 Number of successful extensions: 5280 Number of sequences better than 1.0e-05: 16 Number of HSP's gapped: 5267 Number of HSP's successfully gapped: 16 Length of query: 429 Length of database: 38,378,431 Length adjustment: 109 Effective length of query: 320 Effective length of database: 25,807,025 Effective search space: 8258248000 Effective search space used: 8258248000 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 117 (49.7 bits)