BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0280.0007.p (516 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value BH2378 [R] COG1418 Predicted HD superfamily hydrolase 623 e-178 BS_ymdA [R] COG1418 Predicted HD superfamily hydrolase 597 e-170 lin1436 [R] COG1418 Predicted HD superfamily hydrolase 597 e-170 CAC1816 [R] COG1418 Predicted HD superfamily hydrolase 562 e-160 SA1129 [R] COG1418 Predicted HD superfamily hydrolase 520 e-147 >BH2378 [R] COG1418 Predicted HD superfamily hydrolase Length = 521 Score = 623 bits (1607), Expect = e-178, Method: Compositional matrix adjust. Identities = 319/415 (76%), Positives = 362/415 (87%) Query: 102 DKKMESLERKEEQVANKEKRIDETQQQIDVIYKNQVTELERISNLSIDDARSIILSNVEQ 161 DKK ESL+++EE + K+++I+E +++ I Q ELERIS S ++AR +I + VEQ Sbjct: 107 DKKEESLDKREESLTKKQRQIEEMDSKVEEILNKQQVELERISGFSREEAREVIRAEVEQ 166 Query: 162 EVRHETAQMIKEIEQQAKEEADKKAREIITLAIQRCAADHVAETTVSVVTLPNEEMKXXX 221 E+ HETA +IKE +AKEEADKKAREI++LAIQRC+ADHVAETTVSVV LPN+EMK Sbjct: 167 EMAHETALLIKERVAKAKEEADKKAREILSLAIQRCSADHVAETTVSVVNLPNDEMKGRI 226 Query: 222 XXXXXXXXXALETLTGIDLIIDDTPEAVILSGFDPIRREVARTALEKLVADGRIHPARIE 281 ALETLTGIDLIIDDTPEAVILSGFDPIRRE+ARTALEKLV DGRIHPARIE Sbjct: 227 IGREGRNIRALETLTGIDLIIDDTPEAVILSGFDPIRREIARTALEKLVQDGRIHPARIE 286 Query: 282 EMVEKSRKEVDERIREYGEQATFEVGVHGLHPDLIKILGRLKFRTSYGQNVLKHSMEVAY 341 EMV+K+R+EVDE IREYGEQ TFE+G+HGLHPDLIKILGRLKFRTSYGQNVLKHSMEVAY Sbjct: 287 EMVDKARREVDEAIREYGEQTTFEMGIHGLHPDLIKILGRLKFRTSYGQNVLKHSMEVAY 346 Query: 342 LTGLMAGELGEDIVLAKRAGLLHDIGKALDHEVEGSHVEIGVELAKKYKEHPVVINSIAS 401 L GLMA ELGED+ LA+RAGLLHDIGKA+DHEVEGSHVEIGVELA KYKEHPVVIN+IAS Sbjct: 347 LAGLMAAELGEDVKLARRAGLLHDIGKAIDHEVEGSHVEIGVELATKYKEHPVVINAIAS 406 Query: 402 HHGDCEATSVIAMLVGAADALSAARPGARRETLETYIKRLEKLEEISESFEGVEKSYAIQ 461 HHGD EATS+I+ LV AADALSAARPGARRETLETYI+RLEKLEEISESF+GVEK+YAIQ Sbjct: 407 HHGDTEATSIISSLVAAADALSAARPGARRETLETYIRRLEKLEEISESFDGVEKTYAIQ 466 Query: 462 AGREVRVMVQPEKIDDAEAFRLARDITKMIESELDYPGHIKVTVIRETRAVEYAK 516 AGREVR+MV+P+ +DD A +LARDITK IE ELDYPGHI+VTVIRETRAVEYAK Sbjct: 467 AGREVRIMVKPDIVDDVLAHKLARDITKKIEEELDYPGHIRVTVIRETRAVEYAK 521 >BS_ymdA [R] COG1418 Predicted HD superfamily hydrolase Length = 103 Score = 597 bits (1540), Expect = e-170, Method: Compositional matrix adjust. Identities = 0/415 (0%), Positives = 0/415 (0%) Query: 102 DKKMESLERKEEQVANKEKRIDETQQQIDVIYKNQVTELERISNLSIDDARSIILSNVEQ 161 Sbjct: 106 165 Query: 162 EVRHETAQMIKEIEQQAKEEADKKAREIITLAIQRCAADHVAETTVSVVTLPNEEMKXXX 221 Sbjct: 166 225 Query: 222 XXXXXXXXXALETLTGIDLIIDDTPEAVILSGFDPIRREVARTALEKLVADGRIHPARIE 281 Sbjct: 226 285 Query: 282 EMVEKSRKEVDERIREYGEQATFEVGVHGLHPDLIKILGRLKFRTSYGQNVLKHSMEVAY 341 Sbjct: 286 345 Query: 342 LTGLMAGELGEDIVLAKRAGLLHDIGKALDHEVEGSHVEIGVELAKKYKEHPVVINSIAS 401 Sbjct: 346 405 Query: 402 HHGDCEATSVIAMLVGAADALSAARPGARRETLETYIKRLEKLEEISESFEGVEKSYAIQ 461 Sbjct: 406 465 Query: 462 AGREVRVMVQPEKIDDAEAFRLARDITKMIESELDYPGHIKVTVIRETRAVEYAK 516 Sbjct: 466 520 >lin1436 [R] COG1418 Predicted HD superfamily hydrolase Length = 520 Score = 597 bits (1539), Expect = e-170, Method: Compositional matrix adjust. Identities = 307/414 (74%), Positives = 361/414 (87%) Query: 103 KKMESLERKEEQVANKEKRIDETQQQIDVIYKNQVTELERISNLSIDDARSIILSNVEQE 162 K+ +LERKEE ++ ++++I+E + ++ + + + TELERIS LS ++A+SIIL+ VE+E Sbjct: 107 KREATLERKEESISKRQQQIEEKESKLAEMIQAEQTELERISALSKEEAKSIILNQVEEE 166 Query: 163 VRHETAQMIKEIEQQAKEEADKKAREIITLAIQRCAADHVAETTVSVVTLPNEEMKXXXX 222 + H+TA M+KE E +AKEE+DKKA+ I++LAIQRCAADHVAETTVSVVTLPN+EMK Sbjct: 167 LTHDTAIMVKESENRAKEESDKKAKNILSLAIQRCAADHVAETTVSVVTLPNDEMKGRII 226 Query: 223 XXXXXXXXALETLTGIDLIIDDTPEAVILSGFDPIRREVARTALEKLVADGRIHPARIEE 282 LETLTGIDLIIDDTPEAVILSGFDPIRRE+AR ALEKLV DGRIHPARIEE Sbjct: 227 GREGRNIRTLETLTGIDLIIDDTPEAVILSGFDPIRREIARIALEKLVQDGRIHPARIEE 286 Query: 283 MVEKSRKEVDERIREYGEQATFEVGVHGLHPDLIKILGRLKFRTSYGQNVLKHSMEVAYL 342 MV+K+RKEVDE IRE GEQATFEVG+H +HPDLIKILGRL++RTSYGQNVL HS+EV+ L Sbjct: 287 MVDKARKEVDEHIREVGEQATFEVGIHSIHPDLIKILGRLRYRTSYGQNVLNHSLEVSKL 346 Query: 343 TGLMAGELGEDIVLAKRAGLLHDIGKALDHEVEGSHVEIGVELAKKYKEHPVVINSIASH 402 G++AGELGED+ LAKRAGLLHDIGKA+DHE+EGSHVEIGVELA KYKE+ VVINSIASH Sbjct: 347 AGILAGELGEDVTLAKRAGLLHDIGKAIDHEIEGSHVEIGVELATKYKENDVVINSIASH 406 Query: 403 HGDCEATSVIAMLVGAADALSAARPGARRETLETYIKRLEKLEEISESFEGVEKSYAIQA 462 HGD EATSVIA+LV AADALSAARPGAR ETLE YI+RLEKLEEISES++GVEKSYAIQA Sbjct: 407 HGDTEATSVIAVLVAAADALSAARPGARSETLENYIRRLEKLEEISESYDGVEKSYAIQA 466 Query: 463 GREVRVMVQPEKIDDAEAFRLARDITKMIESELDYPGHIKVTVIRETRAVEYAK 516 GREVR++V+P+ IDD ++RLARDI K IE ELDYPGHIKVTVIRETRAVEYAK Sbjct: 467 GREVRIIVEPDTIDDLSSYRLARDIRKRIEEELDYPGHIKVTVIRETRAVEYAK 520 >CAC1816 [R] COG1418 Predicted HD superfamily hydrolase Length = 514 Score = 562 bits (1449), Expect = e-160, Method: Compositional matrix adjust. Identities = 276/415 (66%), Positives = 339/415 (81%) Query: 102 DKKMESLERKEEQVANKEKRIDETQQQIDVIYKNQVTELERISNLSIDDARSIILSNVEQ 161 DKK + LE++E + +E I E Q I+ +YK + +LE++S L+ ++A++++L V + Sbjct: 100 DKKNDLLEKRENGLNERENSIQERQNTIEQLYKEEREKLEQLSGLTSEEAKNLLLEEVNR 159 Query: 162 EVRHETAQMIKEIEQQAKEEADKKAREIITLAIQRCAADHVAETTVSVVTLPNEEMKXXX 221 E++HE + MIKE+EQ+AKEEADKKAREIIT AIQRCAADHVAE+TV VV+LPN+EMK Sbjct: 160 EIKHEKSIMIKEVEQKAKEEADKKAREIITYAIQRCAADHVAESTVYVVSLPNDEMKGRI 219 Query: 222 XXXXXXXXXALETLTGIDLIIDDTPEAVILSGFDPIRREVARTALEKLVADGRIHPARIE 281 A+ETLTG+DLIIDDTPEAVILSGFDP+RREVA+ ALEKL+ DGRIHPARIE Sbjct: 220 IGREGRNIRAIETLTGVDLIIDDTPEAVILSGFDPVRREVAKVALEKLILDGRIHPARIE 279 Query: 282 EMVEKSRKEVDERIREYGEQATFEVGVHGLHPDLIKILGRLKFRTSYGQNVLKHSMEVAY 341 EMVEK++KEV+ I+E GEQATFE GVHGLH +L ++LGRLK+RTSYGQNVLKHS+EV+Y Sbjct: 280 EMVEKAKKEVENNIKEEGEQATFETGVHGLHSELTRLLGRLKYRTSYGQNVLKHSIEVSY 339 Query: 342 LTGLMAGELGEDIVLAKRAGLLHDIGKALDHEVEGSHVEIGVELAKKYKEHPVVINSIAS 401 L G MA ELG D AKRAGLLHDIGKA+DHEVEG H IG E++KKY E P+++N+I + Sbjct: 340 LAGFMAAELGIDPTFAKRAGLLHDIGKAVDHEVEGPHALIGAEVSKKYHELPLIVNAIGA 399 Query: 402 HHGDCEATSVIAMLVGAADALSAARPGARRETLETYIKRLEKLEEISESFEGVEKSYAIQ 461 HHGD E S+ A+LV AADA+SAARPGARRETLE YIKRLEKLEEI+ S+EGVEKSYAIQ Sbjct: 400 HHGDVEPQSLEAILVQAADAISAARPGARRETLEAYIKRLEKLEEIANSYEGVEKSYAIQ 459 Query: 462 AGREVRVMVQPEKIDDAEAFRLARDITKMIESELDYPGHIKVTVIRETRAVEYAK 516 AGRE+R+MV+PE +DDA + +AR I K IE +L+YPG IKV VIRETRAVEYAK Sbjct: 460 AGREIRIMVKPETVDDAGSIEMARGIVKKIEEQLEYPGQIKVNVIRETRAVEYAK 514 >SA1129 [R] COG1418 Predicted HD superfamily hydrolase Length = 519 Score = 520 bits (1340), Expect = e-147, Method: Compositional matrix adjust. Identities = 272/415 (65%), Positives = 336/415 (80%) Query: 102 DKKMESLERKEEQVANKEKRIDETQQQIDVIYKNQVTELERISNLSIDDARSIILSNVEQ 161 DKK E LE+KE ++ K++++D + + + ELERIS L+ ++A + L VE+ Sbjct: 105 DKKDEILEQKESKIEEKQQQVDAKESSVQTLIMKHEQELERISGLTQEEAINEQLQRVEE 164 Query: 162 EVRHETAQMIKEIEQQAKEEADKKAREIITLAIQRCAADHVAETTVSVVTLPNEEMKXXX 221 E+ + A ++KE E++AKE+ DK A+E++ A+QR AADH +E+TVSVV LPN+EMK Sbjct: 165 ELSQDIAVLVKEKEKEAKEKVDKTAKELLATAVQRLAADHTSESTVSVVNLPNDEMKGRI 224 Query: 222 XXXXXXXXXALETLTGIDLIIDDTPEAVILSGFDPIRREVARTALEKLVADGRIHPARIE 281 LETLTGIDLIIDDTPEAVILSGFDPIRRE+ARTAL LV+DGRIHP RIE Sbjct: 225 IGREGRNIRTLETLTGIDLIIDDTPEAVILSGFDPIRREIARTALVNLVSDGRIHPGRIE 284 Query: 282 EMVEKSRKEVDERIREYGEQATFEVGVHGLHPDLIKILGRLKFRTSYGQNVLKHSMEVAY 341 +MVEK+RKEVD+ IRE GEQATFEV H +HPDL+KI+GRL +RTSYGQNVLKHS+EVA+ Sbjct: 285 DMVEKARKEVDDIIREAGEQATFEVNAHNMHPDLVKIVGRLNYRTSYGQNVLKHSIEVAH 344 Query: 342 LTGLMAGELGEDIVLAKRAGLLHDIGKALDHEVEGSHVEIGVELAKKYKEHPVVINSIAS 401 L ++A ELGED LAKRAGLLHD+GKA+DHEVEGSHVEIGVELAKKY E+ VIN+I S Sbjct: 345 LASMLAAELGEDETLAKRAGLLHDVGKAIDHEVEGSHVEIGVELAKKYGENETVINAIHS 404 Query: 402 HHGDCEATSVIAMLVGAADALSAARPGARRETLETYIKRLEKLEEISESFEGVEKSYAIQ 461 HHGD E TS+I++LV AADALSAARPGAR+ETLE YI+RLE+LE +SES++GVEK++AIQ Sbjct: 405 HHGDVEPTSIISILVAAADALSAARPGARKETLENYIRRLERLETLSESYDGVEKAFAIQ 464 Query: 462 AGREVRVMVQPEKIDDAEAFRLARDITKMIESELDYPGHIKVTVIRETRAVEYAK 516 AGRE+RV+V PE+IDD +++RLARDI IE EL YPGHIKVTV+RETRAVEYAK Sbjct: 465 AGREIRVIVSPEEIDDLKSYRLARDIKNQIEDELQYPGHIKVTVVRETRAVEYAK 519 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.316 0.133 0.359 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 29,012,211 Number of extensions: 1091391 Number of successful extensions: 3713 Number of sequences better than 1.0e-05: 8 Number of HSP's gapped: 3709 Number of HSP's successfully gapped: 8 Length of query: 516 Length of database: 38,378,431 Length adjustment: 111 Effective length of query: 405 Effective length of database: 25,576,357 Effective search space: 10358424585 Effective search space used: 10358424585 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 118 (50.1 bits)