BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0280.0004.p (316 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value PH1026 [E] COG2355 Zn-dependent dipeptidase, microsomal dipeptid... 130 3e-30 AGc4575 [E] COG2355 Zn-dependent dipeptidase, microsomal dipepti... 120 3e-27 SPAC3A11.10c [E] COG2355 Zn-dependent dipeptidase, microsomal di... 111 2e-24 mll3679 [E] COG2355 Zn-dependent dipeptidase, microsomal dipepti... 105 1e-22 AGl875 [E] COG2355 Zn-dependent dipeptidase, microsomal dipeptid... 102 8e-22 >PH1026 [E] COG2355 Zn-dependent dipeptidase, microsomal dipeptidase homolog Length = 320 Score = 130 bits (327), Expect = 3e-30, Method: Compositional matrix adjust. Identities = 75/206 (36%), Positives = 115/206 (55%), Gaps = 7/206 (3%) Query: 98 LSLEGVDGLEGNLFYAQLCFELGVRFMGVTWNYANWAADGVLEQRNGGFTEQGRKLINWC 157 L +EG + +E +L ++ +G+R + +TW+ N DGV E+ GG T G +++ C Sbjct: 107 LGMEGGEPIE-SLDILEIFHSIGLRVLTLTWSLRNQIGDGVFERTRGGLTNFGAEVVGKC 165 Query: 158 NESGMLLDVSHLAPAGFWELAERSVRPFIASHSNAAAVCGHPRNLSDEQIQALIAMDGRI 217 E G+++D+SH+ GFW++ + + P IASHSNA ++C +PRNL+DEQI+A+ DG I Sbjct: 166 EELGIIIDLSHINEQGFWDVLDITGFPVIASHSNAKSLCDNPRNLTDEQIKAIAERDGVI 225 Query: 218 GVTFVPWFVRDGGEAKAEDVLRHIEHICSLGGSGQIMFGSDF-DGIDCW----VEGLEHP 272 G +P FV D E ++HIE++ L G + G DF + W VEGLE Sbjct: 226 GAVAIPAFV-DKDNPTLEKYVKHIEYMVDLVGYKHVGIGFDFVYYLPGWSGKSVEGLEDE 284 Query: 273 GKYAEFAELLLARYPEDAVKGWLHGN 298 K + + L + E VKG N Sbjct: 285 SKIPDLIKALEENFSEKEVKGITFEN 310 >AGc4575 [E] COG2355 Zn-dependent dipeptidase, microsomal dipeptidase homolog Length = 351 Score = 120 bits (301), Expect = 3e-27, Method: Compositional matrix adjust. Identities = 17/340 (5%), Positives = 52/340 (15%), Gaps = 39/340 (11%) Query: 1 VADFHCDVLSKL----QASPD-IDFRKDSRLDVTLDRLLAGGVELQVFAIYL-------- 47 + + + + + V + Sbjct: 11 MVISFRQMAEWENGMLRFGIISTAKIAQDHVIPAIQDAENCVVSAIASRDHARARAVAER 70 Query: 48 -----STRREKASFERILGQIES-FRNQVAGSGGLQWLCWKEEAAEQINTPTGRG----M 97 + + + A + Sbjct: 71 FSVPHAFGSYEEMLASDVIDAVYIPLPTAQHVEWTI-KA---ANAGKHVLCEKPIALKAE 126 Query: 98 LSLEGVDGLEGNLFYAQLCFELGVRFMGVTWNYANWAADGV------LEQRNGGFTEQGR 151 + + N F + + + Sbjct: 127 EIDTLIAARDRNGVIVSEAFMVTYSPVWAKVKELLASGAIGTLKHVQGAFSYFNRDPGNM 186 Query: 152 KLINWCNESGMLLDVSHLAPAGFWELAERSVRPFIASHSNAAAVCGHPRNLSDEQIQALI 211 + I + + + R + ++ + + Sbjct: 187 RNIPELGGGALPDIGVYPTIVTRFATGAEPRRVQATVERDREFGTDIYSSVRADFGTFEL 246 Query: 212 AMDGRIGVTFVPWFVRDGG----EAKAEDVLRHIEHICSLGGSGQIMFGSDFDGIDCWVE 267 + + V G E K+ + F + Sbjct: 247 SFYLATQLASRQLMVFHGTDGYIEVKSPFNANRWGAEEIELTNQAHNQSQVFRFQ-DSRQ 305 Query: 268 GLEHPGKYAEFAELLL-ARYPEDAVKGWLHGNALSFLAKN 306 +A E++ K L +A+ A+ Sbjct: 306 YKLEAEAFARAVRGEGEVVTLENSRKNQLFIDAIYRAAEK 345 >SPAC3A11.10c [E] COG2355 Zn-dependent dipeptidase, microsomal dipeptidase homolog Length = 409 Score = 111 bits (277), Expect = 2e-24, Method: Compositional matrix adjust. Identities = 66/194 (34%), Positives = 100/194 (51%), Gaps = 4/194 (2%) Query: 96 GMLSLEGVDGLEGNLFYAQLCFELGVRFMGVTWNYANWAADGVLE--QRNGGFTEQGRKL 153 M+ +EG+ + G+ + ++LGVR+ + N N AD ++ + N G + GR + Sbjct: 176 SMMGIEGLHQIAGSPSILRQFYDLGVRYATLAHNCDNVFADAAVDGKRTNKGLSPAGRDI 235 Query: 154 INWCNESGMLLDVSHLAPAGFWELAERSVRPFIASHSNAAAVCGHPRNLSDEQIQALIAM 213 + N GM++D+SH P + + SV P SHS+A V HPRN+ D+ + + Sbjct: 236 VREMNRLGMIVDLSHTTPETMHQALDVSVAPAFFSHSSAKGVYDHPRNVPDDVLIRVKET 295 Query: 214 DGRIGVTFVPWFVRDGGE-AKAEDVLRHIEHICSLGGS-GQIMFGSDFDGIDCWVEGLEH 271 DG + V F P F+ E A + V+ HI HI ++ GS I G DFDGID +GLE Sbjct: 296 DGVVMVNFYPAFISPHPENATIDTVVEHIMHIANVTGSYRHIGLGGDFDGIDMVPKGLED 355 Query: 272 PGKYAEFAELLLAR 285 KY + L R Sbjct: 356 VSKYPDLFVKLAER 369 >mll3679 [E] COG2355 Zn-dependent dipeptidase, microsomal dipeptidase homolog Length = 352 Score = 105 bits (261), Expect = 1e-22, Method: Compositional matrix adjust. Identities = 85/301 (28%), Positives = 134/301 (44%), Gaps = 44/301 (14%) Query: 1 VADFHCDVLSKLQASPDIDFRK-----DSRLDVTLDRLLAGGVELQVFAIYLSTRREKAS 55 V D H D L +L S D D K S + L R AGG +FAI+ EKA Sbjct: 9 VFDGHNDTLLRLYQSKDRDVEKLFVEGKSGGHIDLPRAKAGGFAGGMFAIF-PPPVEKAR 67 Query: 56 FERI-------------LGQIESFRNQVAGS--------GGLQWLCWKEEAAEQINTPTG 94 + + + E+ + +A + G +C A + Sbjct: 68 RSAVPLAPSDSEPLPPEVPRAEALNSTMAMASILFRLERAGALAVC---RTAGDVRNAMA 124 Query: 95 RGMLS----LEGVDGLEGNLFYAQLCFELGVRFMGVTWNYANWAADGVL------EQRNG 144 +G ++ +EGV+ ++ L + G+R +G+ W+ N GV Sbjct: 125 KGTIAAVFHIEGVEAIDPELTMLDVLHAAGLRSLGIVWSRPNAFGHGVPFRFPSSPDTGP 184 Query: 145 GFTEQGRKLINWCNESGMLLDVSHLAPAGFWELAERSVRPFIASHSNAAAVCGHPRNLSD 204 G T+ G+ L+ CNE +++D+SHL GF ++A S P +A+HSN A+C H RNL+D Sbjct: 185 GLTDAGKALVKACNELKIMIDLSHLNEKGFRDVAGLSDAPLVATHSNVHALCTHSRNLTD 244 Query: 205 EQIQALIAMDGRIGVTFVPWFVRDGGEAKAEDVL----RHIEHICSLGGSGQIMFGSDFD 260 Q+ A+ G +G+ F F+R+ G A+ L RH++ + G + GSDFD Sbjct: 245 WQLGAIRESGGMVGLNFATGFLREDGRMNADTSLDIMVRHVDSLLQALGEDGVGLGSDFD 304 Query: 261 G 261 G Sbjct: 305 G 305 >AGl875 [E] COG2355 Zn-dependent dipeptidase, microsomal dipeptidase homolog Length = 832 Score = 102 bits (254), Expect = 8e-22, Method: Compositional matrix adjust. Identities = 10/201 (4%), Positives = 34/201 (16%), Gaps = 10/201 (4%) Query: 96 GMLSLEGVDGLEGNLFYAQLCFELGVRFMGVTWNYANWAADGVL------EQRNGGFTEQ 149 + +F EL + + AA + + Sbjct: 150 SAAARILDPAAGAGVFLLAAFRELVAARWRSEGHRPDTAALREILYKQVRGFDINEAALR 209 Query: 150 GRKLINWCNESGMLLDVSHLAPAGFWELAERSVRPFIASHSNAAAVCGHPRNLSDEQIQA 209 L + + + + F +L + + + +L + Sbjct: 210 FCALGLYLLSIELDPNPKPVDKLHFDDLRGVVLHRPVDTTDVDRPEAKQLGSLGSLIGEE 269 Query: 210 LIAMDGRIGVTFVPWFVRDGGEAKAE----DVLRHIEHICSLGGSGQIMFGSDFDGIDCW 265 + + + + D + Sbjct: 270 HDGQYDLVIGNPPWASATGLEDWNLLLTEVHKIARSRLGDGITAPPLPNAVLDLPFVWRA 329 Query: 266 VEGLEHPGKYAEFAELLLARY 286 + + + Sbjct: 330 MRWAKPDAQIIFALHARFLFQ 350 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.321 0.138 0.436 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 24,955,680 Number of extensions: 1091857 Number of successful extensions: 1709 Number of sequences better than 1.0e-05: 14 Number of HSP's gapped: 1694 Number of HSP's successfully gapped: 14 Length of query: 316 Length of database: 38,378,431 Length adjustment: 106 Effective length of query: 210 Effective length of database: 26,153,027 Effective search space: 5492135670 Effective search space used: 5492135670 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 116 (49.3 bits)